• 제목/요약/키워드: Pin-By-Pin Analysis

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Profile Analysis of Proteins Related with Hydrogen Peroxide Response in Strep-tomyces coelicolor (Muller) (Streptomyces coelicolor (Muller)의 과산화수소 대응 반응에 관련된 단백질 양상의 분석)

  • 정혜정;노정혜
    • Korean Journal of Microbiology
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    • v.31 no.2
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    • pp.166-174
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    • 1993
  • Streptomyces coeUc%r (Muller) cells were treated with $100 \mu$M hydrogen peroxide for I hour and proteins synthesized during hydrogen peroxide stress were labeled with L-[$^{35}S$]-methionine. Total cellular proteins were extracted and analyzed by two-dimensional polyacrylamide gel electrophoresis. In exponential growth phase, synthesis of about 100 proteins was increased by hydrogen peroxide treatment. These proteins were named as Pin (£eroxide-inducib]e) proteins and classified into 4 subgroups according to their induction time after hydrogen peroxide treatment. About 60 of them were found to be induced within 20 minutes and maintained throughout I hour of treatment. In stationary growth phase. synthesis of 62 proteins was increased by hydrogen peroxide and 21 of them were the same Pins found in exponential growth phase. Proteins from the mutants which are resistant to hydrogen peroxide were obtained in exponential growth phase and compared with those from the wild type on two-dimensional gel. The three mutants, N7, N9. and N24, were found to have higher constitutive leve]s of ]5, 17, and 15 Pin proteins respectively, than the wild type. 9 of these Pin proteins (D74.7a, E76.0c, E23.3. F50.7, F47.2a. F25.5, G39.6b, G24.0, H39.6a) increased in two of the three mutants and 3 proteins (F39.7, H6I.7. 120.8) increased in all of the three mutants. These proteins might play important roles in the response of S. coelic%r to hydrogen peroxide.

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Study on the UHF-band Variable Attenuator Using the 3-dB Coupler (UHF대역 3-dB 커플러(Coupler)를 이용한 가변 감쇄기(Attenuator)에 대한 연구)

  • 박경태
    • Journal of the Institute of Convergence Signal Processing
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    • v.2 no.2
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    • pp.68-74
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    • 2001
  • A design and implementation method for the UHF-band(800MHz) variable attenuator using the 3dB coupler is proposed. The 90 degree, 3-dB Coupler is used for the variable attenuator. The principal theory for the 3-dB coupler is introduced. The 3-dB Coupler is designed by the mathematical analysis and a computer simulation tool. A PIN diode is used for the variable resistor at UHF-band. The variable attenuator using the 3-dB coupler and the PIN diode is designed and implemented. The measured results for the variable attenuator by a network analyzer show that the insertion loss is below -l0dB, and the continuous variable attenuation range is about 10dB.

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A Study for Rationalization of Lifting Lug Design of Ship Block (선박블록 탑재용 러그구조의 설계합리화를 위한 연구)

  • 함주혁
    • Journal of Ocean Engineering and Technology
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    • v.11 no.4
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    • pp.249-261
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    • 1997
  • A basic study on the lifting lug design has performed through the rational and systematic process. In order to evaluate the proper design-load distribution around lug eye investigation of contact force between lifting lug and shackle pin is performed using non-linear parametric analysis idealized by gap element models. Gap element modeling and nonlinear analysis procedures are illustrated and discussed based on MSC/NASTRAN. Some analysis and design guides are suggested through the consideration of several important effects such as stress distribution pattern, circumferential contact force distribution along the lug eye face, loading share rate between lug main plate and doubler, effect of loading direction, relation between applied force and deflection and size effect of shackle pin radius. Additionally optimum design studies are performed and general trends according to the variation of design parameters are suggested.

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Design of A Waveguide Limiter Having an Improved Attenuation and a Broadened Bandwidth by Using Multiple PIN-Diode Posts (다중 PIN-다이오드 포스트를 이용한, 향상된 감쇄량과 대역폭이 늘어난 도파관 리미터의 설계)

  • Kattak, Muhammad Kamran;Yoo, Seon-woong;Kahng, Sungtek;Yoo, Seongryong;Oh, DongChul;Roh, DonSuk;Yun, Songhyun
    • Journal of Satellite, Information and Communications
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    • v.10 no.3
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    • pp.26-31
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    • 2015
  • This paper deals with a size-reduced Ku-band waveguide limiter. Basically, it passes the signal from 16.125 GHz through 16.375 GHz, but when excessively high power is injected to the input port, it should change to a bandstop filter. Furthermore, it is required to change to bring attenuation by more than 20 dB and 50 dB over a narrow band and the entire passband, respectively. Therefore, in order to meet this requirement, a limiting device is implemented with multiple PIN-diode posts that enable the limiter to be the bandpass filter and stopband one at the off and on states of the PIN-diode switch, respectively. So, the design goes through the equivalent circuit modelling and the geometry is realized in the accurate electromagnetic analysis CAD tool. Finally, the result is discussed to shed light on whether it complies with the aforementioned requirement.

Numerical Study on the Effect of Guide Vane Position and Angle on Heat Transfer and Flow Characteristics of a Pin-fin Channel with a Guide Vane (가이드 베인을 부착한 핀-휜 유동에서 가이드 베인 위치와 입사각에 따른 전열 및 유동 특성에 관한 수치적 연구)

  • Lee, Deukho;Oh, Yeongtaek;Bae, Jihwan;Lee, Changhyeong;Kim, Kuisoon
    • Journal of the Korean Society of Propulsion Engineers
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    • v.23 no.3
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    • pp.35-43
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    • 2019
  • In this study, a guide vane with varying positions and incidence angles was adopted to a pin-fin channel to analyze the change in thermal performance and pressure loss characteristics. A numerical analysis was conducted to investigate the effect of incidence angles and positions on heat transfer and flow characteristics at Re =1400. The results of it were compared those of a pin-fin channel without a guide vane. In case 1 when the incidence angle is $0^{\circ}$, the heat transfer performance is maximized and improved by approximately 5% when compared to the pin-fin channel without the guide vane. In case 2 when the incidence angle is $10^{\circ}$, the pressure loss is minimized and decreased by approximately 1.9% when compared to the pin-fin channel without the guide vane.

A Study on the Chucking of Ferrule using Cone-type Centers and Ball-type Centers in Co-Axial Grinding - Chucking Error Analysis (원추형 센터와 볼형 센터를 이용한 페룰 동축 연삭시 척킹에 관한 연구-척킹 오차 해석)

  • 김동길;박성준;김영태;이상조
    • Journal of the Korean Society for Precision Engineering
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    • v.20 no.2
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    • pp.40-49
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    • 2003
  • Ferrule is widely used as fiber optic connecters. In fiber-optic communications. the shape accuracy such as coaxiality and cylindricity of ferrule affects insertion loss. When coaxial grinding of ferrule supported by two pin. pin alignment and chucking accuracy are very important. In this research, the kinematic behavior of the ferrule centers are investigated in the case where the ferrule is chucked with the cone-shaped center pins and bail centers, With homogeneous coordinate transformation and numerical analysis, the obtained results are as follows: In the case of cone-type center, the alignment errors between center pins alone do not affect the rotation accuracy of ferrule. The alignment errors between center holes cause sinusoidal displacement of ferrule. And the maximum displacement of ferrule centers is proportional to the center pin angle. In the case of ball-type center, the displacements of ferrule centers has similar pattern as cone-type center, and the alignment errors art proportional to ball diameters.

Analysis of Sliding Wear Behavior of Mild Steel According to Hardness of Dissimilar Mating Materials (이종 상대재 경도에 따른 철강재료의 미끄럼 마모 특성 해석)

  • Lee, Han-Young
    • Tribology and Lubricants
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    • v.32 no.6
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    • pp.195-200
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    • 2016
  • This study examines the wear behavior of mild steel pins mated against alloyed tool steel discs in a pin-on-disc type sliding test machine and provides specific clarification regarding the effects of disc hardness on the wear behavior of a mating mild steel pin. The analysis confirms these effects through the observation of differences in the wear rates of the mild steel pins at low sliding speed ranges. These differences occur even though the hardness of the mating disc does not affect the wear characteristic curve patterns for the sliding speeds, regardless of the wear regime. In the running-in wear regime, increasing the hardness of the mating disc results in a decrease in the wear rates of the mild steel pins at low sliding speed ranges. However, in the steady-state wear region, the wear rate of a pin mated against the 42DISC is greater than the wear rate of a pin mated against the 30DISC, which has a lower hardness value. This means that the tribochemical reactivity of the mating disc, which is based on hardness value, influences the wear behavior of mild steel at low sliding speed ranges. In particular, oxides with higher oxygen contents, such as $Fe_2O_3$ oxides, form predominantly on the worn surface of the 42DISC. On the contrary, the wear behavior of mild steel pins at high sliding speed ranges is nearly unaffected by the hardness of the mating disc.

The Effects of the Geometrically Initial Imperfection on Buckling Characteristics of pin-Jointed Single-Layer Lattice Domes (핀접합 단층래티스돔의 좌굴특성에 관한 형상초기부정의 영향)

  • Jung, Hwan Mok
    • Journal of Korean Society of Steel Construction
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    • v.10 no.4 s.37
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    • pp.769-777
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    • 1998
  • That main purpose of this paper is to clarify the effects of geometrically initial imperfection on the buckling characteristics of the pin-jointed single-layer lattice domes with triangular network. Additionally, this study is to get the data that is to formulate the general buckling-strength equation taking geometrically initial imperfection into consideration. Analysis is undertaken by using the frame analysis method which is based on the finite element method dealing with geometrically nonlinear problem.

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A Study of Design and Analysis on the High-Speed Serial Interface Connector (고속 직렬 인터페이스 커넥터의 설계 및 분석에 대한 연구)

  • Lee, Hosang;Shin, Jaeyoung;Choi, Daeil;Nah, Wansoo
    • The Journal of Korean Institute of Electromagnetic Engineering and Science
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    • v.27 no.12
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    • pp.1084-1096
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    • 2016
  • This paper presents method of design and analysis of a high-speed serial interface connector with a data rate of 12.5 Gbps. A high-speed serial interface connector is composed of various material and complex structures. It is very difficult to match the impedance of each discontinuous portion of connector. Therefore, this paper proposes the structure of a connector line that be simplified a connector. In the structure of proposed connector line, this research presents a method for extracting R, L, C and G parameters, analyzing the differential mode impedance, and minimizing the impedance discontinuity using time domain transmissometry and time domain reflectometry. This paper applies the proposed methods in the connector line to the high-speed serial interface connector. The proposed high-speed serial interface connector, which consists of forty-four pins, is analyzed signal transmission characteristics by changing the width and spacing of the four pins. According to the analysis result, as the width of the ground pin increases, the impedance decreases slightly. And as the distance between the ground pin and the signal pin increases, the impedance increases. In addition, as the width of the signal pin increases, the impedance decreases. And as the distance between the signal pin and the signal pin increases, the impedance decreases. The impedance characteristic of initial connector presents ranges from 96 to $139{\Omega}$. Impedance characteristic after applying the structure of proposed connector is shown as a value between 92.6 to $107.5{\Omega}$. This value satisfies the design objective $100{\Omega}{\pm}10%$.

Velocity and Acceleration Error Analysis of Planar Mechanism Due to Tolerances (기계시스템의 공차에 의한 속도 및 가속도 오차의 해석)

  • 이세정
    • Transactions of the Korean Society of Mechanical Engineers
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    • v.18 no.2
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    • pp.351-358
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    • 1994
  • A probabilistic model and analysis methods to determine the means and variances of the velocity and acceleration in stochastically-defined planar pin jointed kinematic chains are presented. The presented model considers the effect of tolerances on link length and radial clearance and uncertainty of pin location as a net effect on the link's effective length. The determination of the mean values and variances of the output variables requires the calculation of sensitivities of secondary variables with respect to the random variables. It is shown that this computation is straightforward and can be accomplished by a conventional kinematic analysis package with minor modification. Thus, the concepts of tolerance and clearance have been captured by the model and analysis. The only input data are the nominal linkage model and statistical information. The "effective link length" model is shown to be applicable to both analytical solution and Monte Carlo simulation. The results from both methods are compared. This paper Ksolves the higher-order kinematic problems for the probabilistic design analysis of stochastically-defined mechanisms.echanisms.