• 한국토양비료학회지
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• 제44권2호
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• pp.200-205
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• 2011

본 실험은 케라틴 단백질인 Pigs hoof를 알칼리 가수분해하였을 때, 그 가수분해물의 아미노산 함량을 알아보고 액상비료로서의 이용성을 고찰하는 한편, 작물에 시비하였을 경우의 영향 등을 평가하여, 식물환경 연구에 관한 기초자료로 활용하고자 수행하였다. 가수분해물의 화학적 특성을 조사한 결과, pH는 6~7, EC는 $10{\sim}15dS\;m^{-1}$, 질소는 1~3% 범위였으며, 기타 성분은 모두 0.2% 미만인 것으로 조사되었다. 가수분해물에 함유된 유리아미노산의 총 함량은 10.18%로 분석되었다. 상추 재배 후 가수분해물을 처리한 모든 처리구의 토양 pH는 초기보다 감소하였으며, 치환성 양이온은 Control보다 가수분해물 처리구에서 증가하는 결과를 보였다. 상추의 엽장과 엽폭은 Control보다 6~16%, 4~15%가 증가되었으며, 특히 Pigs hoof 가수분해물 500배액을 엽면시비한 PHH-2.0 처리구가 가장 높은 결과를 나타내었다. 이러한 결과는 가수분해물의 pH 및 성분함량이 영향을 미쳤기 때문인 것으로 판단된다. 식물체 양분 함량 중 질소는 pigs hoof 가수분해물 500배 희석액을 처리한 PHH-2.0 처리구가 4.19%로 가장 높은 결과를 보였다. 다양한 아미노산을 함유하고 있는 가수분해물을 상추에 처리하였을 때, 상추의 질소 흡수가 촉진되는 것으로 판단된다. 결론적으로, 가수분해 과정을 통해 생성된 pigs hoof 가수분해물에는 작물 생육을 위한 성분과 아미노산이 다량 함유되어 있는 것으로 조사되었다. 또, 가수분해물 엽면시비시 상추의 생육 및 수량 증대효과를 보였으며, pigs hoof 가수분해물 1,000배액 처리가 경제성 및 효과 면에서 가장 적절할 것으로 보인다. 이처럼 케라틴 부산물의 가수분해물은 비료로서의 효과가 인정되었으나, 여러 조건을 통하여 분해율을 상승시킬 수도 있지만, 액상비료로 이용하기 위해서는 경제성, 생산성, 효율성 등을 고려하여 적정 수준을 유지하는 것이 바람직할 것으로 판단된다.

• 한국임상수의학회지
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• 제23권3호
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• pp.272-278
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• 2006

This study was carried out to determine therapeutic the effect of ozonated water therapy on bovine pododermatitis. In addition, bactericidal effect of ozonated water on etiological agent of bovine pododermatitis was examined. The pathohistological examination for the pododermatitis, according to application with ozonated water and ozone ointment was investigated. Thirty healthy cattle were divided two groups(each of 15) : control group(povidone group), treatment group(ozone solution group). Various parameters were evaluated in terms of the lameness score, swelling score, lesion score, WBC count, neutrophil count, pathohistological finding, and antimicrobial action. The decrease of lameness and lesion score were shown in hoof lesions on 14 days after application of ozonated water. Significant decrease of swelling was shown in hoof lesions on 14 days 1Corresponding author after application of ozonated water(p<0.01). In hematological findings, WBC count revealed values within normal range. The number of neutrophils was slightly higher than that of normal, however, this was improved on 14 days after application of ozonated water. In pathohistological findings, recovery was rapid macroscopically and microscopically in the treatment with ozonated water on the hoof lesions and ozonated water was effective. In antimicobial action, bactericidal effect was observed in treatment with ozonated water on the hoof lesions and ozonated water was effective.

• 대한수의학회지
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• 제40권4호
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• pp.719-727
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• 2000

A study was conducted to determine the susceptibility of swine to Korean foot-and-mouth disease virus (FMDV; subtype O, isolated from Chungju province) in April, 2ooo. One holstein cow was inoculated intradermolingually with suspension of homogenized tissue from a Korean native cow naturally infected with Korean FMDY. Infected cow was housed with one susceptible cow and one susceptible pig (contact sentinels). Four additional susceptible pigs were housed in the same room but caged separately (non-contacted sentinels). The contacted pig and cow as well as non-contact pigs developed typical clinical signs after 2, 3, and 7 days post exposure, respectively. We compared neutralizing antibody from the animals to FMDV $O_1$ Lombardy, O Taiwan, $O_1$ Campos, and $O_1$ Manisa after 0, 4, 7, 10, 14, 21, 28 days post challenge and post-exposure. The highest viral neutralization titer could be interpreted that serotype O Korea (Chungju isolate) is antigenically more related to $O_1$ Manisa. In addition, immunohistochemistry was used to further characterize Korean FMDV from tissues of infected pigs. Korean FMDV antigen was observed in the tongue, hoof, esophagus, and tonsil tissues of sentinel pigs. These findings suggest that Korean FMD virus isolated from cattle can be rapidly transmitted to pigs both directly and indirectly contrast field observation in which only cattle were clinically ill.

• 한국임상수의학회지
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• 제23권3호
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• pp.279-285
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• 2006

Thirty dairy cows with pododermatitis were selected and treatment effect of ozone ointment for bovine pododermatitis was investigated. In addition, bactericidal effect of ozone ointment on etiological agent of bovine pododermatitis was evaluated. The pathohistological examination for the pododermatitis, according to application with ozone ointment was investigated. Thirty dairy cows were divided two groups: control group(vaseline group: 15 cows), treatment group(ozone ointment group: 15 cows). Various parameters were evaluated in terms of the lameness score, swelling score, lesion score, WBC, neutrophil, pathohistological finding, and antimicrobial action. As compared with vaseline group, ozone ointment group revealed significant decrease of lameness(p<0.05), swelling(p<0.01) and lesion score(p<0.05) were shown in hoof lesions on 14 days after application. In hematological findings, WBC count revealed slightly high values within normal range before treatment, however, this was 1Corresponding author improved on 14 days after application of ozone ointment. The number of neutrophils was slightly higher than that of normal, however, this was improved on 14 days after application of ozone ointment. In pathohistological findings, normal dermal tissue was found in tissues with pododermatitis on 14 days after application of ozone ointment. In antimicobial action, marked decrease rate of bacteria was observed in feet of all cases treated with ozone ointment. The decreasing rate of bacteria in anaerobic culture was higher than that in aerobic culture.