• 생명과학회지
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• 제19권3호
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• pp.417-421
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• 2009

4, 8, 12, 20 주령의 미성숙 미니돼지에서 방사선과 초음파를 이용하여 복부 주요 장기의 형태학적 특징을 살펴보았다. 방사선 검사는 전반적인 장기의 형태를 확인하는 데 이용되는 가장 기본적이고 단순한 방법이다. 기본 복부방사선 사진에서 이용되는 평가해야 할 항목들은 우선 전반적인 복부세부음영의 정도, 간, 위, 비장, 신장, 방광, 장들의 위치, 크기, 형태, 변연, 후복강 평가이다. 초음파 검사는 이러한 주요 장기들의 내부 실질을 평가하는 데 주로 사용된다. 미니돼지는 개와 고양이와 비교했을 때 유사한 소견을 가진 것을 확인하였다.

• Asian-Australasian Journal of Animal Sciences
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• 제33권2호
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• pp.360-372
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• 2020

Objective: Specific genomic sites can be recognized and permanently modified by genome editing. The discovery of endonucleases has advanced genome editing in pigs, attenuating xenograft rejection and cross-species disease transmission. However, off-target mutagenesis caused by these nucleases is a major barrier to putative clinical applications. Furthermore, off-target mutagenesis by genome editing has not yet been addressed in pigs. Methods: Here, we generated genetically inheritable α-1,3-galactosyltransferase (GGTA1) knockout Yucatan miniature pigs by combining transcription activator-like effector nuclease (TALEN) and nuclear transfer. For precise estimation of genomic mutations induced by TALEN in GGTA1 knockout pigs, we obtained the whole-genome sequence of the donor cells for use as an internal control genome. Results: In-depth whole-genome sequencing analysis demonstrated that TALEN-mediated GGTA1 knockout pigs had a comparable mutation rate to homologous recombination-treated pigs and wild-type strain controls. RNA sequencing analysis associated with genomic mutations revealed that TALEN-induced off-target mutations had no discernable effect on RNA transcript abundance. Conclusion: Therefore, TALEN appears to be a precise and safe tool for generating genomeedited pigs, and the TALEN-mediated GGTA1 knockout Yucatan miniature pigs produced in this study can serve as a safe and effective organ and tissue resource for clinical applications.