• Journal of Sericultural and Entomological Science
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• v.52 no.2
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• pp.155-158
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• 2014

Biological control of Tetranychus urticae by Phytoseiulus persimilis was carried out on tropical plant (Erythrina caffra, Phytolacca dioica) from April to July. Experiments were conducted at Tropical Plant Resources Research Center of Korea National Arboretum. Phytoseiulus persimilis (200 individual/tree) was released once (12. May) to control Tetranychus urticae. We measured the density of leaf-feeding T. urticae at intervals of approximately 2weeks after introducing P. persimilis. The densities of T. urticae were suppressed 10%, in Erythrina caffra and 49% in Phytolacca dioicas after 2 weeks introducing them.

• BMB Reports
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• v.44 no.1
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• pp.64-69
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• 2011

A peroxidase (PD-cP; 0.47 mg/100 g leaves) was purified from autumn leaves of Phytolacca dioica L. and characterized. PD-cP was obtained by acid precipitation followed by gel-filtration and cation exchange chromatography. Amino acid composition and N-terminal sequence of PD-cP up to residue 15 were similar to that of Spinacia oleracea (N-terminal pairwise comparison showing four amino acid differences). PD-cP showed a molecular mass of approx. 36 kDa by SDS-PAGE, pH and temperature optima at 3.0 and $50.0^{\circ}C$, respectively and seasonal variation. The Michaelis-Menten constant ($K_M$) for $H_2O_2$ was 5.27 mM, and the velocity maximum ($V_{max}$) $1.31\;nmol\;min^{-1}$, while the enzyme turnover was $0.148\;s^{-1}$. Finally, the presence of $Ca^{2+}$ and $Mg^{2+}$ enhanced the PD-cP activity, with $Mg^{2+}$ 1.4-fold more effective than $Ca^{2+}$.