• Journal of the Korean Applied Science and Technology
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• v.37 no.5
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• pp.1116-1124
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• 2020

In this study, in order to evaluate the possibility of applying apple leaf extract as a cosmetic material, we conducted an experiment by extracting with 70% ethanol. Apple leaf extract is analyzed by GC/MS, and toxicity is evaluated through polyphenol, flavonoid content and DPPH radical scavenging activity, antioxidant, and cell viability (MTT assay) OK, and nitric oxide (NO). It was confirmed that the NO production amount decreased 4.6 times in the group treated with the apology leaf extract through the measurement of the production inhibition of), and the anti-inflammatory effect was investigated. The total polyphenol content was 78.80 ± 0.25 mg /g and the total flavonoids were 65.25 ± 6.62 mg /g. It was confirmed that the DPPH scavenging activity increased sogonun at 79.8 ± 0.99,% at an extract concentration of 0.25%, 88.13 ± 0.89% at 0.5%, and 96.83 ± 2.00% at 1%. The cell viability was evaluated at 91.19 ± 3.49% even at a high concentration of 1000 ppm, confirming a viability of 80% or more. As a result of component analysis of GC-MS, it can be used as an antioxidant cosmetic material such as catechol (5.65%), DL-Gluciol (12.05%), Ascorbic acid (2.41%), Phytol (13.88%), Hexanoic acid (5.47%). Conceivable. The result of this experiment will be used as an important basic material in the development of natural functional cosmetic materials using apple leaf extract.

• Journal of Life Science
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• v.11 no.6
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• pp.603-611
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• 2001

Phytolalexins are produced in plants affected by various environmental factors such as fungal infection treatment with many chemicals and irradiation by ultraviolet light. When pepper and tobacco bel suspension cultures were grown on a basal MS medium supplemented with 2,4-D(1mg/$\ell$, benzyl adenine(0.001 mg/$\ell$) and 100$\mu$ M jasmonic acid, the production of capsidiol was observed. The total of compound found in pepper plant were around seventy and thirty of them were located intissue-specific manner. 1-propanethiol, $\alpha$-D-xylofuranoside, phenol, hexadecanonic acid ethyl tridecanoate, phytol, linoleic acid and capsidiol are those which have change the production level by treatments, such as the inoculation of Phytophthora capsici Leonian, the metalaxyl treatment and the UV-B irradiation, respectively. The content of capsidiol on inoculation of P. capsici with metalxyl suspension in soil were higher than those of P.capsici without metalaxyl. When the soil dernch of metalaxyl treatment (1$\mu\textrm{g}$/${mu}ell$)was delayed after inoculation, the content of capsidiol were higher than that of before. Irrradiated UB-B the production on capsidiol was identified only at leaf, and contents were the highest for 24 hrs incubation after 20 minutes irradiation.

• Journal of Forest and Environmental Science
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• v.13 no.1
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• pp.134-142
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• 1997

Pectin and tannin analysis were carried out to inverstigate any available components from Pinus densiflora, P. koraiensis, P. thunbergii and P. rigida. To analyze terpenoid components, the essential oils were extracted with steam distillation method from four kinds of pine needles. The essential oil was analyzed by GC and GC-MS spectroscopy. The results were summarized as follows: Pectin content was highest in P. koraiensis with 0.40%, and tannin content was highest in P. koraiensis with 1.05. Major components of P. densiflora needles were ${\alpha}$-pinene, ${\beta}$-caryophyllene, ${\Delta}^3$-carene and phytol. ${\alpha}$-Pinene, ${\Delta}^3$-carene, ${\beta}$-caryophyllene, germacrene D, and camphene were found major components in P. koraiensis. Major components of P. thunbergii needles were ${\beta}$-pinene, ${\alpha}$-pinene, ${\beta}$-caryophyllene and germacrene D. ${\beta}$-Pinene, ${\alpha}$-piene, humulene oxide and ${\alpha}$-elemene were major components in P. rigida. Sabinene and citronellol were infrequent components in P. koraiensis, and ${\alpha}$-pinene oxide was present only in P. rigida. ${\alpha}$-Pinene, limonene, and bornylacetate well known as the main components of green air bath were found in P. densiflora and P. koraiensis.

• Food Science and Preservation
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• v.15 no.3
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• pp.411-420
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• 2008

This study was performed to examine the effect of $\gamma$-irradiation on the volatile organic compounds in Houttuynia cordata Thunb.(H. cordata). 1be volatile compounds of non-irradiated and 10 kGy $\gamma$-irradiated H. cordata were isolated using SDE apparatus and analyzed by GC/MS. For each treatment the number of volatile compounds were detected at 83 and 85, respectively, 1be predominant functional groups of volatile organic compounds from H. cordata were identified as alcohols and ketones. H. cordata was mainly composed of hexahydrofarnesyl acetone (12.81 %), phytol, decanoic acid, dodecanoic acid, octadecanol, caryophyllene oxide, 2-undecanone and menthol. Houttuynum which is characteristic compound of H. cordata was found in all samples and there was no significant difference with irradiation doses. Consequently, $\gamma$-irradiation of H. cordata would be an effective process for sanitation and to increase extraction efficiency.

• Food Science and Preservation
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• v.13 no.1
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• pp.108-114
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• 2006

This study was conducted to determine the volatile flavor compositions of the essential oil and the headspace of Capsella bursa-pastoris Medicus. Essential oil and headspace from the plant were extracted by simultaneous steam distillation extraction (SDE), and solid-phase microextraction(SPME) methods, respectively. Seventy-two compounds including 28 hydrocarbons, 4 aldehydes, 6 ketones, 16 alcohols, 4 esters, 8 acids, and 6 miscellaneous ones were identified in the leaf essential oil extracted by SDE method Sixty-eight compounds including 26 hydrocarbons, 2 aldehydes, 6 ketones, 17 alcohols, 4 esters, 6 acids, and 7 miscellaneous ones were identified in the root essential oil. According to the instrumental analyses the essential oil, phytol ($21.12\%$ in leaves, $20.94\%$ in roots) was the most abundant compound Alcohols, esters, and acids were main groups of the essential oil. On the other hand, thirty-eight compounds including 18 hydrocarbons, 3 aldehydes, 3 ketones, 9 alcohols, 2 esters, 3 miscellaneous ones were identified in the leaf headspace by SPME. In root headspace, thirty-three compounds including 16 hydrocarbons, 2 aldehydes, 1 ketone, 9 alcohols, 3 esten;, and 2 miscellaneous ones were identified. Hydrocarbons($44.02\%$ in leaves, $56.98\%$ in roots) were the main components of the headspace of Capsella bursa-pastoris Medicus.

• Journal of Life Science
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• v.22 no.9
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• pp.1166-1172
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• 2012

To investigate potential medicinal or functional uses of Eriobotrya japonica, this study focused on the isolation and identification of antioxidant compounds from Eriobotrya japonica leaves. Various solvents were extracted from the leaves, and their scavenging effect on 2,2-diphenyl-1-picrylhydrazyl (DPPH) radicals was measured, in addition to their superoxide dismutase-like activity, polyphenol compounds, and flavonoid content. Ethyl acetate extract exhibited the strongest scavenging effect in a 0.2 mM solution of DPPH ($63.24{\pm}2.20%$, $81.83{\pm}2.10%$, and $93.15{\pm}2.31%$ in 0.3, 0.7, and 1.0 mg/ml sample concentrations, respectively). The antioxidant effect of the ethyl acetate extract and methanol extract were generally stronger than that of n-hexane extract. The extracts were further purified by repeated silica gel column chromatography. The antioxidant compounds were identified as phytol, ${\beta}$-sitosterol, and (-)-loliolide using GC/MS.

• Natural Product Sciences
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• v.14 no.2
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• pp.100-106
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• 2008

Column chromatographic separation of the MeOH extract from the aerial parts of Schizonepeta tenuifolia Briquet led to the isolation of twelve terpenes (1 - 11 and 17), four phenolics (13 - 16) and a hexenyl glucoside (12). Their structures were determined by spectroscopic means to be (-)-pulegone (1), piperitenone (2), p-cymene-3,8-diol (3), schizonepetoside A (4), schizonepetoside C (5), (+)-spatulenol (6), ursolic acid (7), $2{\alpha}$,$3{\alpha}$,$24{\alpha}$,-trihydroxyolean-12en-28oic acid (8), $5{\alpha}$,$8{\alpha}$-epidioxyergosta-6,22-diol-$3{\beta}$-ol (9), stigmast-4-en-3-one (10), ${\beta}-sitosterol$ (11), (Z)-3-hexenyl-1-O-${\beta}$-D-glucopyranoside (12), rosmarinic acid (13), apigenin-7-O-${\beta}$-D-glucopyranoside (14), luteolin-7-O-${\beta}$-D-glucuronopyranoside (15), hesperidin (16) and trans-phytol (17). Compounds 2, 3, 8, 9 and 12 were for the first time isolated from S. tenuifolia Briq.

• Korean Journal of Plant Resources
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• v.26 no.3
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• pp.355-361
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• 2013

The present study attempts to evaluate antioxidant activities of extracts from Abeliophyllum distichum. Nakai flower. The samples were collected in Jangyyeon-myeon, Goesan-gun, Korea and extracted with either hot-water or ethyl acetate (EtOAC). In DPPH, hydroxyl radical scavenging activity and $Fe^{2+}$ chelating activity of EtOAC extracts were 93.41%, 98.43%, and 7.38%, while those of hot-water extracts were 86.93%, 41.33% and 47.68% at 200 ${\mu}g/ml$, respectively. In ${\varphi}X$-174 RF I plasmid DNA cleavage assay, the protective effects of EtOAC and hot-water extracts against oxidative DNA damage were 82% and 17% at 200 ${\mu}g/ml$, respectively. Both extracts showed the protective effect of DNA migration by oxidative stress in intracellular DNA migration assay. Both extracts had no cytotoxity in NIH3T3 cells. Several polyphenolic compounds were identified such as 2-methoxy-benzoic acid, vanillic acid, phytol and pulegone by GC/MS. These results indicated that extracts of Abeliophyllum distichum Nakai flower showed antioxidant activities and protective activities against oxidative DNA damage and showed the possibility to be used as an effective natural antioxidants.

• Korean Journal of Plant Resources
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• v.26 no.6
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• pp.718-725
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• 2013

Components extracted from 7 species and 18 populations of Chrysosplenium in Korea were compared and analyzed using GC-MS analysis. 57 components (${\geq}80%$ quality) were identified, of which neophytadiene, palmitic acid and phytol were found at all the taxa. Percentage composition of isolated extracts showed a clear difference in components type and GC-MS profile. On the basis of that result, data matrix was made and cluster analysis using UPGMA was conducted. From the result of cluster analysis, two groups were recombined; one with alternate leaves comprised C. japonicum in Ser. Alternifolia and C. flagelliferum in Ser. Flagellifera and the other with opposite leaves gradationally comprised C. psuedofauriei in Ser. Sinica, C. ramosum in Ser. Oppositifolia and C. sphaerospermum, C. valdepilosum, C. flaviflorum in Ser. Pilosa. These chemotaxonomic results agreed in general with those of existing studies on external morphology and molecular. In conclusion, chemical composition can be an useful characters in understanding the relation analysis among interspecific and intraspecific complex with the help of cluster analysis of 7 species and 18 populations of Chrysosplenium in Korea.

• Natural Product Sciences
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• v.10 no.6
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• pp.335-340
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• 2004

The chromatographic separation of the MeOH extract of the aerial parts from Aster hispidus (Compositae) led to the isolation of eight compounds. Their structures were established by spectroscopic methods to be ${\beta}-amyrin$ (1), oleanolic acid (2), (2R)-1, 2-O-(9Z, 12Z, $15Z-dioctadecatrienoyl)-3-O-{\beta}-D-galactopyranosyl\;glycerol$ (3), trans-phytol (4), 9, 12, 15-octadecatrienoic acid (5), kaempferol (6), 3,5-dicaffeoyl quinic acid (7), 3,4-dicaffeoyl quinic acid (8) and kaempferol-3-O-rutinoside (9). Compounds 1, $3{\sim}6$ and 9 showed non-specific moderate cytotoxicity against five human tumor cell lines $(5.44{\sim}23.51\;{\mu}g/ml)$. The other compounds were of marginal activity against tested five human cancer cell lines $(9.05{\sim}>30.0\;{\mu}g/ml)$.