• 한국정밀공학회지
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• 제25권1호
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• pp.145-150
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• 2008

One of the unresolved questions in articular cartilage biomechanics is the magnitude of the dynamic modulus and tissue compressive strains under physiological loading conditions. The objective of this study was to characterize the dynamic modulus and compressive strain magnitudes of bovine articular cartilage at physiological compressive stress level and loading frequency. Four bovine calf shoulder joints (ages 2-4 months) were loaded in Instron testing system under load control, with a load amplitude up to 800 N and loading frequency of 1 Hz, resulting in peak engineering stress amplitude of ${\sim}5.8\;MPa$. The corresponding peak deformation of the articular layer reached ${\sim}27%$ of its thickness. The effective dynamic modulus determined from the slope of stress versus strain curve was ${\sim}23\;MPa$, and the phase angle difference between the applied stress and measured strain which is equivalent to the area of the hystresis loop in the stress-strain response was ${\sim}8.3^{\circ}$. These results are representative of the functional properties of articular cartilage in a physiological loading environment. This study provides novel experimental findings on the physiological strain magnitudes and dynamic modulus achieved in intact articular layers under cyclical loading conditions.

• 대한의용생체공학회:의공학회지
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• 제16권3호
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• pp.325-330
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• 1995

혈압에 의한 안쪽벽의 변형을 집중현상으로 인한 동백 전단부의 변형율 gradient의 심각성은 잔유 변형율에 의해 감소되거나 없을 수 있다는 가정을 실험적으로 시도했다. 잔유변형율과 압력에 의한 변형율을 측정하여 잔유변형율이 원주변형율에 끼치는 영향을 알아 보았다. 결과로서 변형율 gradient를 줄이거나 없을수도 있으나 negative gardient가 평균적으로 나타났다. 즉 전체변형율(원주변형율+잔유변형율)은 안쪽벽이 낮고 바깥쪽이 높은 것으로 나타났다.

• Safety and Health at Work
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• 제10권2호
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• pp.219-223
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• 2019

Background: Ice cooling vests can cause tissue damage and have no flexibility. Therefore, these two undesirable properties of ice cooling vest were optimized, and the present study was aimed to compare the impact of the optimized ice cooling vest and a commercial paraffin cooling vest on physiological and perceptual strain under controlled conditions. Methods: For optimizing, hydrogel was used to increase the flexibility and a layer of the ethylene vinyl acetate foam was placed into the inside layer of packs to prevent tissue damage. Then, 15 men with an optimized ice cooling vest, with a commercial paraffin cooling vest, and without a cooling vest performed tests including exercise on a treadmill (speed of 2.8 km/hr and slope of %0) under hot ($40^{\circ}C$) and dry (40 %) condition for 60 min. The physiological strain index and skin temperature were measured every 5 and 15 minutes, respectively. The heat strain score index and perceptual strain index were also assessed every 15 minutes. Results: The mean values of the physiological and perceptual indices differed significantly between exercise with and without cooling vests (P < 0.05). However, the difference of the mean values of the indices except the value of the skin temperature during the exercises with the commercial paraffin cooling vest and the optimized ice cooling vest was not significant (P > 0.05). Conclusions: The optimized ice cooling vest was as effective as the commercial paraffin cooling vest to control the thermal strain. However, ice has a greater latent heat and less production cost.

• Journal of Microbiology
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• 제44권1호
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• pp.113-120
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• 2006

Candida tropicalis was treated with ultraviolet (UV) rays, and the mutants obtained were screened for xylitol production. One of the mutants, the UV1 produced 0.81g of xylitol per gram of xylose. This was further mutated with N-methyl-N'-nitro-N-nitrosoguanidine (MNNG), and the mutants obtained were screened for xylitol production. One of the mutants (CT-OMV5) produced 0.85g/g of xylitol from xylose. Xylitol production improved to 0.87 g/g of xylose with this strain when the production medium was supplemented with urea. The CT-OMV5 mutant strain differs by 12 tests when compared to the wild-type Candida tropicalis strain. The XR activity was higher in mutant CT-OMV5. The distinct difference between the mutant and wild-type strain is the presence of numerous chlamvdospores in the mutant. In this investigation, we have demonstrated that mutagenesis was successful in generating a superior xylitol-producing strain, CT-OMV5, and uncovered distinctive biochemical and physiological characteristics of the wild-type and mutant strain, CT-OMV5.

• 한국식물병리학회:학술대회논문집
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• 한국식물병리학회 2003년도 정기총회 및 추계학술발표회
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• pp.87.2-87
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• 2003

Long-term repeated culturing of biocontrol agents on a certain medium often results in reduced biocontrol efficacy and altered physiology. Effect of culturing media on biocontrol ability and physiological state of Burkholderia gladioli strain B543 was investigated. Over 20 times repeated cultivation of B. giadioli strain B543 on Kings B medium or nutrient agar medium showed improved biological control of cucumber damping-off caused by Pythium ultimum, while one time cultivation on KB or NA did not. The repeated cultivation also induced the physiological changes of the biocontrol agent such as antifungal activity and the production of protease and siderophore. Our result indicates that adaptation to proper culturing medium can alter biocontrol ability and must consider in optimizing the use of biocontrol agents.

• 한국미생물·생명공학회지
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• 제23권6호
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• pp.777-780
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• 1995

A strain US50-3 producing water-soluble red pigment was isolated from the pond separating oil from water near the oil storage tanks. The strain US50-3 was identified as a strain of Serratia marcescens considering its morphological and physiological characteristics, and DNA G+C contents. It showed a little difference comparing to the Type strain and was considered to be another biotype strain.

• The Plant Pathology Journal
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• 제23권3호
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• pp.161-165
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• 2007

Cultivation of a biocontrol agent on a certain medium often results in reduced biocontrol efficacy and alters physiological state. In our previous study, Burkholderia gladioli strain B543 with long-term subculture on tryptic soy agar resulted in significantly reduced biocontrol ability against cucumber damping-off caused by P. ultimum. Therefore, we investigated the influence of laboratory culturing media on biocontrol activity and physiological state of Burkholderia gladioli strain B543 by using long-term repeated culture on a certain medium. When isolate B543 were successionally cultured on King's B agar (KBA), tryptic soy agar, nutrient agar (NA), or soil extract agar more than 20 times, the isolate cultured on KBA or NA showed a significantly enhanced biocontrol efficacy and higher population density in the rhizosphere of cucumber compared to that of the others. However, the isolates cultured on KBA more than 20 times showed the lowest production of protease, siderophore, or antifungal substance(s), measured by skim milk agar, Chrome-Azurol-S agar, and potato dextrose agar amended with 10% of the culture filtrate, respectively. Our results suggest that adaptation to proper culturing medium can alter biocontrol ability and physiological state, and we must consider laboratory media in optimizing the use of biocontrol agents.

• 한국축산식품학회지
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• 제42권6호
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• pp.996-1008
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• 2022

Lactic acid bacteria are representative probiotics that have beneficial effects on humans. Nineteen strains among the 167 single strains from kimchi was selected and their physiological features were investigated. The selection of a strain was based on strong enzyme (lipase, α-amylase, and α-glucosidase) inhibitory activities and anti-obesity effects in the adipocytes. For the final selection, the strain Lactiplantibacillus plantarum KC3 was tested for its potential as a starter. To assess its functionality, a freeze-dried culture of L. plantarum KC3 was administered to a diet-induced obese mouse model receiving a high-fat diet. The animal group administered with L. plantarum KC3 showed significant body weight loss during the 12-week feeding period compared to the high-fat control group. This study investigated the physiological characteristics of selected strain and evaluated its potential as an anti-obesity probiotic in mice.

• 한국미생물·생명공학회지
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• 제22권2호
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• pp.164-168
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• 1994

Identification of the Actinomycetes isolate strain No. 1372, a producer of Actinomycin X$_{2}$ was performed by using ISP method.l The strain, designated as No. 1372, was identified as Streptomyces floridae based on its morphological, physiological and biochemical characteristics. The highest production of the antibiotics by the strain was achieved in a fermentation medium containing soluble starch, yeast extract, (NH$_{4}$)SO$_{4}$, K$_{2}$HPO$_{4}$, NaCl$_{2}$, CaCO$_{3}$, and trace element.

• 한국미생물·생명공학회지
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• 제24권2호
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• pp.155-159
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• 1996

The strain which produces 2-phenylethanol (PEA) from styrene oxide was isolated from soil samples near Ulsan PO/SM plant. The isolated strain was identified as Pseudomonas putida through its morphological, physiological characteristics, and DNA G+C contents. Its metabolic pathway from styrene oxide to 2-phenylethanol was studied and it was found that styrene oxide was transformed to phenylacetaldehyde, to 2phenylethanol (PEA), and then to phenylacetic acid by this strain.