• 대한화장품학회지
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• 제45권1호
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• pp.9-17
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• 2019

본 연구에서는 천연 보습인자의 구성 성분인 L-${\alpha}$-아미노산을 피부에 주기적으로 반복 도포하여 일반적으로 알려진 보습뿐 만 아니라 L-${\alpha}$-아미노산을 피부 톤과 결의 개선제로 활용하였다. L-${\alpha}$-아미노산의 알킬기(R)의 극성과 비극성에 따라 두 그룹으로 나누어 각질케어 효능을 확인하였다. 그 결과, 극성 알킬기를 갖는 L-${\alpha}$-아미노산 콤플렉스(L-${\alpha}$-아미노산-콤플렉스-1)가 비극성 알킬기를 갖는 L-${\alpha}$-아미노산 콤플렉스(L-${\alpha}$-아미노산-콤플렉스-2)에 비해 21% 향상된 효능을 나타내었다. 임상 실험을 위해 20 - 40대 11인의 여성에게 실험군으로서 L-${\alpha}$-아미노산 콤플렉스-1을 포함한 에멀전을 오른쪽 뺨에 8주간 매일 도포하고 대조군으로서 L-${\alpha}$-아미노산 콤플렉스-1를 포함하지 않은 에멀전을 왼쪽 뺨에 같은 방법으로 도포하였다. 피부 톤의 개선은 $JANUS^{(R)}$ 장비를 통해 측정하고 이미지분석 소프트웨어를 이용하여 분석하였으며, 피부 결의 개선은 phaseshift rapid in-vivo measurement of the skin (PRIMOS)장비를 이용하여 기기적으로 측정하고 분석하였다. 그 결과, 피부 톤 11.7%, 피부 결 6.7%의 개선을 확인하였다. 또한 피험자에게 심미적으로 느껴지는 개선에 대해 설문조사를 실시하여 피부개선의 체감도를 바탕으로 기기측정 결과를 통해 나타난 L-${\alpha}$-아미노산 도포의 효능을 평가하였다. 본 연구 결과를 통해, 약산성(pH 6.5)조건에서 피부 자극을 유발하지 않음과 동시에 생리학적인 개선을 통한 피부 톤과 결의 개선을 기대할 수 있으며, 화장품 소재로서 심미적 만족감을 줄 수 있는 물질로 L-${\alpha}$-아미노산이 활용될 수 있을 것이라 생각된다.

• 생명과학회지
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• 제33권12호
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• pp.1002-1014
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• 2023

다양한 질병의 발병 및 진행을 예방하기 위해 오랫동안 사용되어 온 대계는 항산화 활성을 포함하여 광범위한 생리 활성을 갖는 것으로 보고되었으나, 간세포에서의 효능에 대한 연구는 여전히 미비한 실정이다. 본 연구에서는 간세포에서 대계 에탄올 추출물(EECJ)의 항산화 활성 및 관련 기전을 조사하기 위해 인간 간세포암종 HepG2 세포주를 선택하였고, 산화적 스트레스를 모방하기 위해 H₂O₂를 처리하였다. 본 연구 결과에 따르면 EECJ는 H₂O₂가 처리된 HepG2 세포에서 세포 생존율의 감소와 LDH의 방출을 유의적으로 억제하였다. EECJ는 또한 세포 형태학적 변화의 관찰, 유세포 분석 및 LC3의 발현 결과에서 입증된 바와 같이 H₂O₂에 의해 유도된 자가포식을 유의하게 감소시켰으며, H₂O₂에 의해 유도된 세포사멸과 세포주기의 교란을 약화시켰다. 이는 H₂O₂에 노출된 HepG2 세포가 EECJ에 의하여 지속적인 세포 분열과 증식이 유지되고 있음을 의미한다. 또한, EECJ가 강력한 항산화 활성을 가지고 있음을 세포 내 ROS 및 미토콘드리아 슈퍼옥사이드 생산의 차단으로 확인하였으며, 이는 H₂O₂에 의해 감소된 세포 내 GSH 함량의 회복과 MnSOD 및 GPx의 발현 및 활성을 보존과 연관성이 있었다. 비록 EECJ에 함유된 활성 성분의 분석과 in vivo 동물 모델에서의 검증이 요구되지만, 본 연구의 결과는 EECJ가 산화적 스트레스로 인한 간세포의 손상을 예방하고 치료하기 위한 잠재적인 후보로 사용될 수 있음을 의미한다.

• Journal of Acupuncture Research
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• 제17권2호
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• pp.187-208
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• 2000

By the activation of ovary hormone, many morphological changes occur in the epithelial cell lines and muscle cells in rat uterus. These two cells in uterus are important to the implantation of embryo, maintaining pregnancy and starting parturition. One important change associated with the morphological change of these two cells in uterus is the change on prostaglandin(PG) metabolism. Its presence and synthesis in endometriurn and myometrium in uterus affects estrous cycle and the start of embryo implantation in uterus. It also performs as an important modulator in parturition. So the abnormally weak expression of PG causes difficulty during labor and over-expression causes pre-term labor. PG biosynthesis starts from either free or liberated arachidonic acids from membrane phospholipid by phospholipase. Such arachidonic acids are converted into PG catalyzed by Cyclooxygenase. Under normal physiological condition, Cyclooxygenase-1(COX-1) having 602 units of amino acids controls the synthesis of PG. It acts as a local hormone regulating vasomodulation of blood flow, flexible muscle movement, increasing the blood permeability and contributing the protective role in preserving integrity of the stomach lining and Cyclooxygenase-2 (COX-2) is induced by the inflammation, pregnancy and increased its expression until parturition. Lipid metabolite like PG is located in uterine and expression of COX-2 increased with pregnancy. Increased expression of COX proteins in epithelial cells and myometrial cells are told to increase the muscle contractility in uterus but decreased right after the labor in rat. It is a good sign indicating that COX proteins are deeply related to the start of labor. Currently, Several studies report the use of PG and COX-2 inhibitor as medication for controlled abortion or to prevent pre-term labor but they entail various side-effects. Our study proposed to suggest use of acupuncture as an another mediator to control abortion or pre-term labor without causing unnecessary side-effects by those medicines. Two acupuncture sites, LI-4 & SP-6 were selected due to their known efficacy. From the immunohistochemical staining of COX-2, normal expression of COX-2 protein in nonpregnant SD rat's uterus revealed that COX-2 protein was primarily detected in the lumina epithelial lining and in the epithelial cell lining contacting the stromal cells. High resolution optical microscopic scanning revealed distinguishable staining in the myometrial mucosa. LI-4 acupuncture administered nonpregnant rat's uterus showed strong expression for COX-2 in endometrium contacted with lumina epithelial lining of rat uterus and in myometrial mucosa. Stromal cells showed more staining than untreated nonpregnant rat's uterus and stronger staining in stromal cells contacting myometrial layer compared to untreated nonpregnant rat's uterus. SP-6 acupuncture administered nonpregnant rat's uterus showed weak expression for COX-2 in myometrial layers and stromal cells but no staining was visible in lumina epitheliai and glandular epithelial cells. Few stromal cells and myometrial mucosa were positively stained for COX-2. Pregnant SD rat's uterus was also immunostained for COX-2 expression after 18 days of pregnancy. Unlike to untreated nonpregnant rat's uterus, luminal epithelial cells were not positively stained for COX-2 but stronger staining for COX-2 was revealed in stromal cells. LI-4 acupunctured SD rat's uterus had very strong expression of COX-2 in luminal epithelial lining. Few stromal cells showed stronger positive COX-2 staining and myometrial layers also showed more expression than untreated pregnant rat. SP-6 acupuncture administered pregnant SD rat's uterus showed positive expression of COX-2 in epithelial cells of luminal mucosa layer but weaker than that of LI-4 acupuncture treatment's case. However, strong positive staining was revealed in stromal mucosa and myometrial layers. Virgin SD rat's uterus motility index during LI-4 acupuncture was 66.52 % (Prob〉T = 0.0197) compared to its motility before the acupuncture treatment but the motility index was slighdy elevated up to 79.58 % (Prob〉T = 0.1175) after the acupuncture. During the SP-6 acupuncture treatment for 30 minutes, uterus motility index was 90.52 % (Prob〉T = 0.1832) showing lesser decrement but consequently reached similar motility index decreasal to 79.95 % (Prob〉T = 0.0215) after the acupuncture treatment as LI-4 showed. LI-4 acupuncture tend to be a quick treatment to reducing the uterus motility in a virgin rat but eventually both two acupuncture administration created very similar reduction of uterus motility seeing the index after the both acupunctures. The uterus movement monitored during the LI-4 acupuncture administered for 30 minutes, Pregnant SD rat showed decreased motility down to 77.90 % (Prob〉 T = 0.0076) compared to uterus motility before the acupuncture and it continuously decreased down to 71.81 %(Prob〉T = 0.0214) after the removal of needle. The statistical analysis using paired t-test showed significance difference for both two motility indexs at =0.05. SP-6 acupuncture administered to pregnant SD rat also had similar pattern of decreasing uterus motility index down to 74.70 % (Prob〉T = 0.1730) during the initial 30 minutes acupuncture administration and it was continuously lowered to 71.52 % (Prob〉T = 0.0155) after the acupuncture. The paired t-test resuit for SP-6 suggest prompt response of uterus motility index to the SP-6 acupuncture treatment but consequently reached same level of inducing the motility reduction as LI-4 at =0.05 level.