• Asian-Australasian Journal of Animal Sciences
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• v.22 no.7
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• pp.955-961
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• 2009

The aim of this study was to determine the specific polymorphic sites in cattle breeds and inter- and interbreed genetic variation among breeds and to develop a databank of Turkish native cattle mtDNA using sequence analysis. The entire D-loop region was analyzed based on DNA sequences in Turkish Grey, East Anatolian Red, South Anatolian Red, and Anatolian Black native breeds. In total, 68 nucleotide differences were observed at 26 different sites. The variable positions consisted of 22 transitions, two transversions, and two insertions, but no deletions. Haplotype number, haplotype diversity, nucleotide diversity, and mean number of pairwise difference values were found to be 17, 0.993, 0.00478, and 4.275, respectively. In addition, a phylogeny was developed by comparison among cattle populations for which the entire D-loop sequence was available. A high level of genetic variation was observed within and among the native cattle breeds.

• International Journal of Industrial Entomology and Biomaterials
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• v.5 no.1
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• pp.103-108
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• 2002

We describe here the cDNA sequence and mRNA expression of a novel serine pretense from the firefly, Pyrocoelia rufa. The 771 bp cDNA encodes for 257 amino acid residues. The deduced protein of P. rufa serine pretense gene contains the catalytic triad and six-conserved cysteine residues. Alignment of the deduced protein of P. rufa serine pretense gene showed 47.4% protein sequence identity to known coleopteran insect Rhyzopertha dominica midgut trpsin-like enzyme. Northern blot analysis revealed that the P. rufa serine pretense is specifically expressed in the midgut of P. rufa larvae.

• Korean Journal of Veterinary Research
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• v.57 no.4
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• pp.249-252
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• 2017

Rabies virus (RABV) causes a neurological disease in warm-blooded animals that is nearly always fatal. In this study, we analyzed the matrix (M) genes in 10 Korean street RABV strains isolated from two Provinces during 2011-2013. The M genes in these 10 Korean strains were highly conserved during 1999-2013. Phylogenetic analysis revealed they were closely related to the M genes of RABVs isolated in northeastern China. Specific amino acid substitutions were identified in the KRVB1206, KRVF1301, and BV9901PJ strains. However, functional domains, including those involved in virus production and pathogenicity, were conserved in all 10 strains.

• Proceedings of the Korean Society for Applied Microbiology Conference
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• 2000.10a
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• pp.118-124
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• 2000

Zn-resistant Methylosobacterium strains were isolated from several non-polluted soil samples collected in all over Japan. Zn-resistant Methylosobacterium strains were predominantly detected in all soil samples and they were also characterized as a UV-tolerant bacteria. The MIC test revealed that the isolates have high zinc resistance in comparison with that of reference Methylosobacterium strains obtained from culture collections. The 16S rDNA-based phylogenetic analysis showed that all strains were divided into two clusters designated as cluster A and cluster B in the present study. All isolates were distributed only in the cluster A. The phylogenetic clustering also well coincided with the differences in the pattern of carbon source utilization.

• Journal of Species Research
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• v.9 no.3
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• pp.181-190
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• 2020

Monoraphidium subclavatum (FBCC-A409) of this study clustered in the same clade as Messastrum gracile strains in the 18S rDNA phylogeny. Compared to Messastrum gracile, Monoraphidium subclavatum did not form a colony, and the curvature of the cell was slightly curved or slightly crescent-shaped. This result means that the genus Monoraphidium is still based on the morphospecies concept, and was not monophyletic and not distinguishable as a separate genus. Two Stichococcus-like strains of this study (NIBRCL0000114567, NIBRCL0000114571) belong to Deuterostichococcus epilithicus and Pseudostichococcus monallantoides respectively in phylogenetic analysis using 18S rDNA sequences. These two species are consistent with recent research in the morphology and the genetic analyses using 18S and ITS rDNA sequences. We reported M. subclavatum, D. epilithicus, and P. monallantoides as newly recorded species in Korea.

• The Korean Journal of Zoology
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• v.34 no.3
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• pp.265-288
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• 1991

한국산 털보톡토기과(Collembola, Insecta)의 계통유연관계를 알아보기 위해 이 과에 속하는 3개속(Sinella, Entomobrya, Homidia)의 6종과 가시톡토기과의 1종(Tomocerus kinoshitai)에 대하여 모서식과 형태적 조사 외에 전기영동을 이용한 효소분석을 시리시하였다. 이들 모든 조사에서 32개의 형태형질과 33개의 모서식형질 및 80개의 효소유전자 형질을 얻어 분지분석하였는데, 이중 효소유전자에 대해서는 UPGMA방법도 적용하였다. 이러한 분석 결과 다양한 분지도를 얻었으나, Homidia종들은 언제나 두 개의 무리로 분리되었으며, Homidia munda의 두 아종이 가장 유연관계가 높았다. 반면에 Homidia koreana의 두 개체군은 다소 분리되어 같은 종으로 보기 의심스럽다는 소견을 얻었다. 또한 형태형질분석으로 얻은 계통수에서 가시톡토기과의 Tomocerus종이 털보톡토기과의 속들 사이에 끼어들어 나타나는데 이것은 형질에 따라 비중을 달리두는 평가방법이 아니기 때문에 나타난 것으로 추측된다. 본 연구 결과 대체로 외부형질과 모서식, 그리고 효소유전자의 세가지 유형의 형질들을 종합 분석할 때 도출되는 계통수가 자연분류에 가장 가까운 근연성을 보이는 것으로 판단되었다.

• The Korean Journal of Zoology
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• v.37 no.3
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• pp.343-351
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• 1994

The partial nucleotide sequences of 185 ribosomal RNA gene were compared for six cnidarian species (one belongs to class Hvdrozoa: Coryne pusilla. Five belong to class Anthozoa: Beflonella rigida from Octocorallia; Anemonia sulcutu, Anthopfeura kurognne, An thopleura midori from Hexacorallia: Cerianthus filiformis from Ceriantipatharial. The aligned sequence data were used to test the hypothesis on the anthozoan phylosenv by using the distance matrix method and parsimony method. The phvlosenetic inferences resulting from these methods indicate that the anthozoan is a monophvletic group and support the three subclass scheme (Octocorallia, HexBcorallia, Ceriantipatharial within class Anthozoa. The result also indicates that ceriantipatharian is more primitive than the other groups and that family Actiniidae is a monophvletic group within the anthozoan. However, the present analysis does not clearly indicate the phvlogenetic relationships of species among genera.

• Journal of Plant Biology
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• v.31 no.2
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• pp.83-89
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• 1988

The Fucales plants have very homogenous taxonomic characteristics at order level, but show many morphological variatations at species or subspecies levels. This study is one of the serial works to obtain more taxonomic data than morphotaxonomic ones and to clarify the taxonomic ranks and characteristics of Korean Fucales plants through physiological and biochemical analyses. Total lipid compositions of Korean Fucales plants were analyzed by the 3-dimensional TLC method. Major components of phospholipids (PA, PC, PG, PE, DPG, LPE, LPC), neutral fat TG and sterols were commonly contained in each species. Unknown lipids X1, X2 and X3 were contained in genus Sargassum, but Hizikia was lacking X3 and Myagropsis was lacking both X2 and X3. The latter contained unique X8. From the view-point of the phylogeny of lipid metabolism, Sargassum might be more closely related to Hizikia than to Myagropsis. A variant of S. patens from Seongsan showed minute metabolic difference from that of the typical plant; but a variant of S. serratifolium did not show any significant difference from that of the typical plant.

• The Korean Journal of Mycology
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• v.43 no.2
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• pp.88-91
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• 2015

Studies on the diversity of indigenous fungi were conducted in the central districts of South Korea from 2008 to 2013. During the studies, potentially unrecorded agarics were collected. All specimens collected in this study were examined morphologically and phylogenetic analysis was also performed. They were identified as Galerina sideroides and Gymnopus luxurians. These fungi have never been reported in South Korea. We report them here with detailed descriptions and figures.

• Animal cells and systems
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• v.12 no.4
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• pp.261-267
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• 2008

The human chromosomes 2, 7, 12 and 17 show genomic homology around Hox gene clusters, is taken as evidence that these paralogous gene families might have arisen from a ancestral chromosomal segment through genome duplication events. We have examined protein data from vertebrate and invertebrate genomes to analyze the phylogenetic history of multi-gene families with three or more of their representatives linked to human Hox clusters. Topology comparison based upon statistical significance and information of chromosome location for these genes examined have revealed many of linked genes coduplicated with Hox gene clusters. Most linked genes to Hox clusters share the same evolutionary history and are duplicated in concert with each other. We conclude that gene families linked to Hox clusters may be suggestion of ancient genome duplications.