• Title/Summary/Keyword: Phylogenetic diversity

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A report of unrecorded bacterial species of Korea isolated in 2016, belonging to the family Deinococcaceae and Planctomycetaceae

  • Kim, Dong-Uk;Kim, Ju-Young;Cha, Chang-Jun;Kim, Wonyong;Kim, Myung Kyum
    • Journal of Species Research
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    • v.7 no.1
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    • pp.9-12
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    • 2018
  • In 2016, as part of a larger effort to discover indigenous prokaryotic species in Korea, we isolated the family Deinococcaceae and Planctomycetaceae as unrecorded bacterial species. From the high 16S rRNA gene sequence similarity (>98.5%) and formation of a robust phylogenetic clade with known species, it was determined that each strain was a distinct bacterial species. There are no official reports that these two species have been described in Korea; therefore, the bacterial strains of Deinococcus and Blastopirellula are described for the first time in Korea. Gram reaction, colony and cell morphology, basic biochemical characteristics, and isolation sources are also described in the species description section.

Phylogenetic Analysis of Bacterial Diversity of Enhanced Biological Phosphorus Removal Activated Sludge by Isolation and Cloning of 16S rDNA

  • Nakamura, Kazunori;Hanada, Satoshi;Kamagata, Yoichi;Kawaharasaki, Mamoru
    • Proceedings of the Korean Society for Applied Microbiology Conference
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    • 2000.10a
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    • pp.109-117
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    • 2000
  • Bacterial community structure composing enhanced biological phosphorus removal (EBPR) activated sludge was analyzed phylogenetically by cloning 165 rDNA after direct DNA extraction. Then, this result was compared with 165 rDNA sequences of randomly isolated bacterial species. The results clearly showed that there are no coincidence between the sequences retrieved directly from activated sludge and those of isolated strains, suggesting that many important bacteria are hidden in activated sludge because of the difficulty in isolation and culture of them.

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Complete Mitogenome of the Russian Sturgeon Acipenser gueldenstaedtii (Acipenseriformes; Acipenseridae)

  • Kim, Keun-Yong;Lee, Sang-Yoon;Song, Ha-Yeun;Park, Chul-Hong;Nam, Yoon-Kwon
    • Fisheries and Aquatic Sciences
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    • v.12 no.1
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    • pp.35-43
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    • 2009
  • Sturgeons and paddlefishes are frequently referred to as 'living fossils' among the actionpterygian lineage. They are increasingly facing threats to their existence because of various anthropogenic pressures. In this study, we present the complete mitogenome sequence of the Russian sturgeon Acipenser gueldenstaedtii (Acipenseriformes; Acipenseridae). The mitogenome showed highly homogeneous molecular features compared to previously known vertebrate mitogenomes. Phylogenetic tree inferred from concatenated protein-coding and tRNA genes unambiguously revealed the monophyly of A. gueldenstaedtii, Acipenser stellatus, and Huso huso. Genetic information of the endangered A. gueldenstaedtii will provide baseline data needed to develop molecular markers for stock identification and assessment of population diversity and also to develop future conservation strategies.

Characterization of a Zygomycete Fungus, Mortierella minutissima from Freshwater of Yeongsan River in Korea

  • Nguyen, Thi Thuong Thuong;Lee, Hyang Burm
    • The Korean Journal of Mycology
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    • v.44 no.4
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    • pp.346-349
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    • 2016
  • During a survey of fungal diversity of the order Mortierellales, a zygomycete strain, EML-YS717-1, was isolated from a freshwater sample collected from the Yeongsan River in Gwangju, Korea. Based on its morphological characteristics and a phylogenetic analysis of the internal transcribed spacer (ITS1 and ITS2) and 5.8S rDNA sequences, the strain was identified as Mortierella minutissima. To the best of our knowledge, M. minutissima, has not previously been authentically reported in Korea.

Isolation and Characterization of Blakeslea trispora Isolated from Gut of Grasshopper and Soldier Fly Larva in Korea

  • Nguyen, Thi Thuong Thuong;Lee, Hyang Burm
    • The Korean Journal of Mycology
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    • v.44 no.4
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    • pp.355-359
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    • 2016
  • During a survey of fungal diversity in insect guts in Korea, two fungal strains, EML-PGH2 and EML-PUKI88, were isolated from the gut of grasshopper and soldier fly larvae inhabiting the bulrush plants at a pond located in the Chonnam National University Arboretum, Gwangju, Korea. Based on their morphological characteristics and a phylogenetic analysis of the internal transcribed spacer (ITS1 and ITS2) and 5.8S rDNA sequences, the strains were identified as Blakeslea trispora. To our knowledge, the zygomycete species B. trispora has not been previously described in Korea.

Molecular Characterization and Morphology of Two Endophytic Peyronellaea Species from Pinus koraiensis in Korea

  • Deng, Jian Xin;Paul, Narayan Chandra;Li, Mei Jia;Seo, Eun-Young;Sung, Gi-Ho;Yu, Seung-Hun
    • Mycobiology
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    • v.39 no.4
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    • pp.266-271
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    • 2011
  • Species of Phoma and its allies were isolated during a survey on the diversity of endophytic fungi associated with pine trees in Korea. Based on the phylogenetic analyses of internal transcribed spacer and ${\beta}$-tubulin gene sequences, two Phoma-like species from the needles of Pinus koraiensis were identified as Peyronellaea calorpreferens and P. glomerata. They were also morphologically identified based on the previous descriptions. Here, we report P. calorpreferens and P. glomerata being present in Korea as endophytic fungi in Pinus koraiensis.

New Records of Two Agarics: Galerina sideroides and Gymnopus luxurians in South Korea

  • Jang, Seokyoon;Jang, Yeongseon;Kim, Jae-Jin
    • The Korean Journal of Mycology
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    • v.43 no.2
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    • pp.88-91
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    • 2015
  • Studies on the diversity of indigenous fungi were conducted in the central districts of South Korea from 2008 to 2013. During the studies, potentially unrecorded agarics were collected. All specimens collected in this study were examined morphologically and phylogenetic analysis was also performed. They were identified as Galerina sideroides and Gymnopus luxurians. These fungi have never been reported in South Korea. We report them here with detailed descriptions and figures.

A New Record on Umbelopsis vinacea and Mucor hiemalis f. corticola Isolated from Korea

  • Das, Kallol;Ha, Ki-Hun;Suh, Sang-Jae;Lee, Seung-Yeol;Jung, Hee-Young
    • The Korean Journal of Mycology
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    • v.48 no.3
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    • pp.197-207
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    • 2020
  • In the screening of fungal diversity, two strains were collected from the soil of Yeongcheon and dissected guts from the bodies of Chinese rice grasshopper (Oxya chinensis), Chinese grasshopper (Acrida cinerea), and Far eastern devil grasshopper (Oedaleus infernalis) from Daejeon in Korea. They were identified as Umbelopsis vinacea (KNU-YC-1801B) and Mucor hiemalis f. corticola (KNU-20F7, KNU-20F8, KNU-20F9). Multigene phylogenetic analyses of the internal transcribed spacer (ITS) regions, and large subunit (LSU) sequence data confirmed two unreported taxa along with their morphology. The results of molecular phylogeny firmly supported the detailed description and illustration for each taxon. As far as we know, both Umbelopsis vinacea and Mucor hiemalis f. corticola are the first reported taxa in Korea.

The first record of nine bacterial species belonging to the phylum Proteobacteria in Korea

  • Kim, Dong-Uk;Kang, Myung-Suk;Kim, Ju-Young;Kim, Myung Kyum
    • Journal of Species Research
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    • v.6 no.3
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    • pp.214-223
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    • 2017
  • As part of a larger study with the aim to discover indigenous prokaryotic species in Korea, nine bacterial strains were isolated and assigned to the phylum Proteobacteria in 2016. High 16S rRNA gene sequence similarity (>98.5%) and formation of a robust phylogenetic clades with known species indicated that each strain belongs to an independent and predefined bacterial species. This is the first report of these nine species in Korea: two strains of the Methylobacterium, two strains of the Microvirga, one strain of the Pantoea, and four strains of the Psychrobacter, all within the Proteobacteria. Gram reaction, colony and cell morphology, basic biochemical characteristics, and isolation sources are also described in the species description section.

Characterization of Two New Records of Mucoralean Species Isolated from Gut of Soldier Fly Larva in Korea

  • Nguyen, Thi Thuong Thuong;Duong, Tham Thi;Lee, Hyang Burm
    • Mycobiology
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    • v.44 no.4
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    • pp.310-313
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    • 2016
  • While surveying the diversity of fungi of the order Mucorales, two isolates, EML-PUKI12-1 and EML-PUKI06-1, were obtained from the gut of soldier fly larvae inhabiting the bulrush at a pond located in the Chonnam National University Arboretum, Gwangju, Korea. The isolates were confirmed as Mucor irregularis and Mucor fragilis species, respectively, based on the morphological characteristics and phylogenetic analysis of rDNA internal transcribed spacer region. Such mucoralean species belonging to undiscovered taxa has not previously been described in Korea.