• 제목/요약/키워드: Photoreceptor

검색결과 121건 처리시간 0.023초

Retinal Development and Opsin Gene Expression during the Juvenile Development in Red Spotted Grouper (Epinephelus akaara)

  • Kim, Eun-Su;Lee, Chi-Hoon;Lee, Young-Don
    • 한국발생생물학회지:발생과생식
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    • 제23권2호
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    • pp.171-181
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    • 2019
  • To produce healthy and stable seed production, we need to obtain information and understand vision that affects behavior of red spotted grouper. We examined their expression and retinal development during the juvenile development. Short-wavelength sensitive opsin (SWS2), a cone photoreceptor, began to be expressed from lens and ear vesicle formation stage and its expression increased until 10 days after hatching (dah). In case of middle-wavelength sensitive opsin (MWS), its expression was detected at 3 dah and reached the highest level at 21 dah. The expression of long-wavelength sensitive opsin (LWS) was first observed from 3 dah and their expression decreased thereafter. Rhodopsin, a rod photoreceptor, was found to be expressed from 2 dah and its expression reached the highest level at 50 dah. The outer nuclear layer (ONL), inner nuclear layer (INL) and ganglion cell layer began to differentiate at 2 dah, while choroid first appeared at 4 dah so that the eyes became black. These results indicate that the development of retina mostly completes around 4 dah. It seems that the development of the retina and the expression of the opsin genes are closely related to the behavior such as hunting prey, considering that the timing of the completion of the development of the retina, the timing of gene expression, and the timing of completion of yolk absorption are similar.

돌연변이가 야기된 돼지 로돕신 유전자를 지닌 형질전환동물의 생산 (The Production of Transgenic Mouse Harboring Mutated Pig Rhodopsin Gene)

  • 김도형;김진회;이훈택;정길생
    • 한국가축번식학회지
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    • 제18권3호
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    • pp.191-197
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    • 1994
  • 광수용기에서 특이적 발현을 유도하는 로돕신 유전자에 존재하는 다양한 점 돌연변이 의해서 일반적으로 색소성 망막염을 초래한다. 이 질병의 측징은 광수용기 세포의 퇴화로 인한 광수용기의 기능상실을 초래하고, 결과적으로 시각상실을 유발한다. 로돕신 유전자는 광수용기의 바깥분절에 존재하며, 빛 에너지를 흡수하여, 시각반응을 야기한다. 본 실험에 사용된 로돕신 유전자는 총 12.5kb의 돼지 로돕신 게놈 유전자로서, 망막에서 특이발현을 유도하는 4kb의 프로모터, 돌연변이를 지닌 5.6kb의 유전자, 그리고 poly A site로 구성되어 있다. 호르몬 투여에 의해 과배란이 유기된 C57BL/6J 생쥐 난자의 웅성전핵에 미세주입법에 의해 도입한 후, 위임신한 생쥐의 난관에 이식하였다. 그 결과 태어난 산자로부터 돌연변이 로돕신 유전자를 지닌 6마리의 형질전환동물을 PCR과 Southern blot analysis를 통하여 확인할 수 있었다. 또한, 이 형질전환 동물들은 로돕신 유전자를 그 자손에게 안정적으로 전달하는 것을 확인하였다. 본 연구는 광수용기 퇴화의 원인규명 및 치료방법을 연구하기 위하여 돌연변이를 지닌 질환모델동물을 생산하는데 있다. 이들 형질전환 동물은 로돕신 유전자의 돌연변이에 의해 야기되는 시각상실의 기전에 관한 연구와 사람의 장님을 치료하기 위한 질환모델동물로서 유용하게 이용될 것이다.

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UV Actions in Plant Photomorphogenesis -Induction and Amplification of Anthocyanin Synthesis in Broom Sorghum-

  • Shichijo, Chizuko;Yatsuhashi, Hiroko;Hashimoto, Tohru
    • Journal of Photoscience
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    • 제9권2호
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    • pp.338-340
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    • 2002
  • In broom sorghum, Sorghum bicolor Moench, UV causes anthocyanin synthesis having action peaks in UVA and UVB regions. We previously reported that UV induces anthocyanin synthesis through UVB photoreceptor and phytochrome activated by UV. Furthermore, UVA and UVB amplify phytochrome-induced anthocyanin synthesis (PIAS). Our action- spectroscopic research indicated that a UV -receptor for amplification of PIAS is likely to be the same or same type of UVB photoreceptor for induction of anthocyanin synthesis. UVA-amplification of PIAS can be explained by the action of a cryptic red light signal (CRS), an amplification factor for PIAS produced by a distinct phytochrome-species activated by UVA. We suggest that UVA photoreceptors are not involved in anthocyanin synthesis in the broom sorghum.

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${\tau}-H_2P_c$/oxadiazole 유기이층막의 감광특성 연구 (III) (A study on the electrophotographic properties of ${\tau}-H_2P_c$/oxadiazole photoreceptor (III))

  • 오승현;박종국;신태현;김영조;박구범;이덕출
    • 대한전기학회:학술대회논문집
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    • 대한전기학회 1994년도 하계학술대회 논문집 C
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    • pp.1397-1399
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    • 1994
  • The purpose of this study is to measure the electrophotographic characteristics of double-layered organic photoreceptor made by dip-coating method, and to evaluate an application for the laser beam printer drum. From the result, electrophotographic characteristics of the photoreceptor were depend on the thickness of the CTL and the initial surface potential.

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무금속프탈로시아닌 색소계를 이용한 전자사진 감광체의 개발에 관한 연구 (A Study on the Development of Electrophotographic Photoreceptor by Metal free-Phthalocyanine Dye Sensitized System)

  • 정은실;김영순;정평진
    • 한국재료학회지
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    • 제10권1호
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    • pp.7-14
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    • 2000
  • 본 연구는 무금속프탈로시아닌 색소계를 이용한 전자사진 감광체의 개발에 관한 것이다. 전하발생물질로서는 각종 형태의 무금속프탈로시아닌을 사용했고, 결합제로서는 각종 폴리머를 사용했으며, 전하이동물질로서는 히드라존유도체나 아연착화합물을 사용했다. 전하발생물질로서 사용한 ${\alpha}-$, ${\beta}-$, x형의 프탈로시아닌중에서는 x형의 무금속프탈로시아닌($x-H_2Pc$)의 경우가 가장 좋은 감도를 보였다. 전하발생물질로서는 $x-H_2Pc$를, 전하수송물질로서는 히드라존유도체를 사용했을 때, 다른 감광체들과 비교하여 73.1%의 높은 $1.50lux{\cdot}sec$의 좋은 감도를 보였다.

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A Study on Photoreceptor by Using the Effect of Additives

  • 유진;김영순;유국현
    • Bulletin of the Korean Chemical Society
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    • 제22권7호
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    • pp.709-715
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    • 2001
  • We have been studied photosensitization mechanism's additive effect, of perylene 3,4,9,10-tetracarboxyl-diimide and X-phthalocyanine (charge generation materials), using the photochemical and photoelectrochemical approach. It was found that the photoreceptor on the excited state reacts with metal oxide, which creates the charge transfer on the interface of SnO2/electrolyte. In the electrode (X5P1) made of five X-phthalocyanine and single perylene 3,4,9,10-tetracarboxyldiimide layers, the cathodic photocurrent of X-phthalocyanine in the 400-600 nm region was increased by the addition of perylene 3,4,9,10-tetracarboxyldiimide. The maximum wavelength of fluorescence of perylene 3,4,9,10-tetracarboxyldiimide showed no dependence on the temperature. The addition of 4-dibenzylamino-2-methylbenzaldehyde diphenylhydrazone known as charge transport material was represented as decreasing photocurrent for X-phthalocyanine and perylene 3,4,9,10-tetracarboxyldiimide, respectively. In the electrode (X1P1) made of single X-phthalocyanine and single perylene 3,4,9,10-tetracarboxyldiimide layers, an anodic photocurrent of about 10.5 nA was generated by addition of hydroquinone at 550 nm. And the characteristic of photoinduced discharge was shown to decrease by a factor of 5 and the speed of dark decay was increased by a factor of 1.2.

Subcellular Localization of GTP Binding Protein in Stentor coeruleus

  • Park, Phun-Bum;Song, Pill-Soon
    • Journal of Photoscience
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    • 제7권1호
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    • pp.31-34
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    • 2000
  • The heterotrichous ciliate Stentor coeruieus shows a step-up photophobic response to visible light In the previous paper, the existence of GTP-binding proteins was confirmed by using the antisera against the carboxy terminal decapeptide of transducin $\alpha$ subunit. The photoreceptor, stentorin, is localized in the pigment granule. If the immunoreactive G-protein directly interacts with the photoreceptor stentorin, the G-protein expected to be located in the pigment granule rather than plasma membrane. To elucidate the function of the immunoreactive G-protein, the localization of the G-protein in Stentor coeruleus was studied. The results suggest that this G-protein is located in the myoneme involved in the contraction and extension of the cell rather than in the pigment granule.

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비정질 박막에 대한 도핑 조건의 영향 및 미세구조와 I-V 연구 (Effect of Dopping Conditions on a-Se Thin-Films : Microstructural and I-V Study)

  • 박성광;박지군;강상식;공현기;김진섭;남상희
    • 한국전기전자재료학회:학술대회논문집
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    • 한국전기전자재료학회 2001년도 추계학술대회 논문집
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    • pp.492-496
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    • 2001
  • Due to their better photosensitivity in X-ray, the amorphous selenium based photoreceptor is widely used on the X-ray conversion materials. It was possible to control the charge carrier transport of amorphous selenium by suitably alloying a-Se with other elements(e,g. As, Cl). In this paper, We investigated dopants(As, Cl) composition rate to improve dark resistivity and transport properties of charge carrier in amorphous selenium using by direct X-ray conversion material. Alloying a-Se with As inhibits the recrystallization of a-Se but introduces undesirable deep hole traps. then doping with Cl(in the ppm range) compensates for the deep hole traps. We investigated their composition rate in various doping conditions and then obtained optimum dopant composition rate. The result was Se-As 0.3%-Cl 30 ppm and X-ray Sensitivity was 0.57 pc/pixel$.$mR at 137 $\mu\textrm{m}$ x 137 $\mu\textrm{m}$ Pixel area.

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프탈로시아닌계 광전도성 유기박막의 제조에 관한 연구 (A study on the preparation of phthalocyanine optoelectric thin films)

  • 박구범;조기선;이덕출
    • E2M - 전기 전자와 첨단 소재
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    • 제7권5호
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    • pp.409-416
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    • 1994
  • A double layered photoreceptor using phthalocyanine dye was made by dip-coating method. The under cutting layer(UCL) was coated with A1$\_$2/O$\_$3/ or polyamide, and the charge generation layer(CGL) was formed by .tau.-type metal-free phthalocyanine. The oxadiazole was used as a charge transport layer(CTL) and polycarbonate and poly(vinyl butyral) was employed as a host polymer. The .tau.-H$\_$2/Pc had an absorption peak around 780nm, which coincided with the emitting wavelengths of GaAlAs diode lasers. Maximum charge acceptance of CTL that gives thickness of 12.mu.m was -900V by corona charge of -6.0kV. In photo-induced discharge measurements, residual potential was less than -20V and sufficient for ordinary use, and sample films using of poly(vinyl butyral) was showed good charge retention. In printing test, drum that was employed polycarbonate as a host polymer showed the good print quality.

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