• 한국환경과학회지
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• 제6권6호
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• pp.697-709
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• 1997

Sequencing Batch Reactor(SBR) experiments for organics and nutrients removal have been conducted to find an optimum anaerobic/anoxic/aerobic cycling time and evaluate the applicability of oxidation-reduction potential(ORP) as a process control parameter. In this study, a 61 bench-scale plant was used and fed with night-soil wastewater in K city which contained TCODcr : 10, 680 mg/l, TBm : 6, 893 mg/l, $NH_4^+-N$ : 1, 609 mg/l, $PO_4^{3-}-P$ : 602 mg/l on average. The cycling time In SBRs was adjusted at 12 hours and 24 hours, and then certainly included anaerobic, aerobic and inoxic conditions. Also, for each cycling time, we performed 3 series of experiment simultaneously which was set up 10 days, 20 days and 30 days as SRT From the experimental results, the optimum cycling time for biological nutrient removal with nlght-soil wastewater was respctively 3hrs, 5hrs, 3hrs(anaerobic-aerobic-anoxic), Nitrogen removal efficiency was 77.9%, 77.9%, 81.7% for each SRT, respectively. When external carbon source was fed in the anoxic phase, ORP-bending point indicating nitrate break point appeared clearly and nitrogen removal efficiency increased as 96.5%, 97.1%, 98.9%. Phosphate removal efficiency was 59.8%, 64.571, 68.6% for each SRT. Also, we finded the applicability of ORP as a process control parameter in SBRs.

• 한국수산과학회지
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• 제35권4호
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• pp.348-355
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• 2002

To know characteristics of water quality in Saemankeum area, we were investigated the water quality of surface layer from July of 1999 to June of 2000. The concentrations of COD and chlorophyll a were in the range of $0.64\~6.40$ (mean 1.96)mg/L, $1.95\~51.55$ (mean 11,07)$mg/m^3$, respectively. The annual mean concentrations of DIN, DIP were found to be 21.182 $\mu$g-at/L and 0,655 $\mu$g-at/L respectively, which were exceeding second grade of seawater quality standard. The nitrogen ratio to the phosphorus was lower than 1. Therefore, nitrogen was playing an important role in phytoplankton growth as limiting factor in study area. Mean values of eutrophication index were exceeding 1, which was the eutrophication criteria. Especially Mankyung and Dongjin estuary were shown over 10 as eutrophication index. Therefore, Saemankeum area could be evaluated to possibility area for eutrophication. Released rate for ammonia nitrogen and phosphate phosphorus from sediments were 62.92 ${\mu}g-at/m^3/hr$ and 6.71 ${\mu}g-at/m^3/hr$, respectively.

• 해양환경안전학회지
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• 제22권7호
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• pp.829-835
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• 2016

부영양화를 일으키는 대표적인 영양물질인 질소와 인을 제거하기 위하여 많은 연구들이 진행되어오고 있다. 본 연구에서는 질소와 인을 제거하기 위하여 해수 및 해수염에 존재하는 마그네슘과 칼슘을 사용하여 스트루바이트와 수산화인회석을 만들어 침전을 시켰다. 실험의 목적은 해수와 해수염을 사용하여 pH와 농도의 변화에 따른 영양염의 제거율을 비교평가 하였다. 하수의 실험조건에서 해수를 사용한 결과 인의 제거율은 90 %, 질소의 제거율은 50 %로 나타났다. 또한 pH 9, 질소와 인의 농도 10 mM, ${Mg/PO_4}^{3-}$, ${NH_4}^+$의 비율 2의 조건에서 해수염을 사용하여 실험한 결과 질소의 제거율은 90 %, 인의 제거율은 70 %로 나타났다. 상대적으로 인의 제거율이 높은 이유는 해수를 사용한 경우 질소와 인의 몰 농도의 차이에서 비롯되었으며, 해수염을 사용한 경우 해수에 포함된 칼슘이 인과 반응하여 수산화인회석으로 침전 제거되었다고 할 수 있다. 수중의 질소와 인을 제거를 위하여 해수와 해수염을 사용한 결과 높은 제거율을 나타내었다.

• 한국물환경학회지
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• 제23권2호
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• pp.244-250
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• 2007

This study was carried out to obtain the optimum design factors for an eletrolytric flotation reactor. When the effluent of the leachate treatment facility was treated under the condition of 10 volts, 30 minutes, at the Al-Al electrode system; COD removal efficiency was 45%, and total phosphorus removal efficiency was 98%. The high removal efficiency was caused by the fact that phosphate was removed by leaching $Al^{3+}$ from two electrodes. The leachate containing high ammonium nitrogen concentration was treated by a batch test under the condition of 60 minutes reaction time and added chloride ion; ammonium nitrogen removal efficiency was 89%. This high efficiency was affected by added chloride ion to wastewater. To find the optimum current density and voltage of the leachate containing chloride ion (ratio of $Cl^-/NH_4-N$ is 11) a electrochemical polarization curve was used. These values were found to be $4.5mA/cm^2$ and about 2.1 V, respectively. When C-Al electrode system was used at a batch test, the total nitrogen removal efficiency was increased by 1.8 to 3.3 times, compared to Al-Al electrode system due to high $Cl_2$ gas production.

• 한국환경과학회지
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• 제10권3호
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• pp.179-185
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• 2001

To investigate daily variations of water mass distribution in the west and the east parts of Jeju Island, we implemented observations from June 20 to 22, 1997. (The results are as follows : Temperatures in the east and the west were similar in the surface, but $15.02^{\circ}C$ and $13.8^{\circ}C$ in the bottom, respectively.) In the east and west, the surface and the deep salinities are 33.81, 33.34 and 34.33, 34.11 respectively. The east shows higher temperature and salinity than the west. The distributions of nitrate in the east and the west were very similar in the surface regions.(In the deep regions, however, the east shows higher than the west.) The east shows higher phosphates than the west both in surface and deep regions. The silicate had similar distributions to nitrate. Chlorophyll-a of the west was twice higher than that of the east. The daily variations of temperature and salinity were largest at 30m depth, and pH was not much changed in the depth and the pH in day was higher than that at night. The variations of nutrients were much changed in the depth, and nitrate and phosphate at night were higher than those in day. Daily variations of silicate, however, was not much changed in the observation times. Ratio of N/P in the east and the west were 13 and 16, respectively, so that the west was higher than the east. However the ratios were two times higher both in the east and the west in day, when planktons are more active than at night. Si/P ratio showed little different between day and night and between surface and deep regions. The correlation of nitrogen and phosphorus was high (plus relation), and the correlation of AOU, nitrogen and phosphorus was high(plus relation) in the east, however, not high in the west.

• ALGAE
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• 제17권2호
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• pp.95-104
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• 2002

Population dynamics of Anabaena and the anatoxin-a concentration were monitored with physicochemical parameters at 3 sites in the lower Naktong River from May to September in 2000. Total 4 species of Anabaena (A. flosaquae, A. smithii, A. ucrainica and A. mucosa) were identified with morphological characterisitcs. Anabaena flos-aquae was most abundant among the populations. The standing crop of Anabaena ranged from 10 to 11,220 cells · $ml^{-1}$ and biomass of Anabaena more 1,000 cells · $ml^{-1}$ was obseved once at St. Mulgeum and St. Seonam, twice at St. Hagueon out of total 9 samplings. There were not significant correlations between the standing crop of Anabaena and other physicochemical parameters such as temperature, nitrate, total nitrogen, phosphate, total phophorus and N/P ratios. The frequency of trichomes with akinetes was low and ranged from 0 to 4% in the total Anabaena population and A. smithii showed highest frequency of 2.8% among all species. The population at St. Seonam showed highest frequency of 1.4% among all sampling sties. The population in September showed the highest frequency of 3.0% among all sampling period. The frequency of trichomes with heterocysts was low and ranged from 1 to 87% inthe total Anabaena population and A. smithii showed highest frequency of 55.1% among all species. The population at St. Mulgeum showed highest frequency of 17.6% among all sampling sites. The population in August showed the highest frequency of 21.4% among all sampling period. The frequency of trichomes with akinetes and/or heterocysts was not related to all the physicochemical parameters of temperature, nitrate, total nitrogen, phosphate, total phosphorus and N/P ratios. The anatoxin-a concentations were determined in algal materials dominated by Microcystis and Anabaena from June though August by derivatization using 7-fluoro-4-nitro-2, 1,3-benzoxadiazole (NBD-F) and HPLC analysis with fluorimetric detection. All the concentrations were below the detection limit of 0.1 ㎍ · $l^{-1}$ in the present study.

• Journal of Microbiology and Biotechnology
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• 제23권1호
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• pp.92-98
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• 2013

Microalgal cultivation using wastewater is now regarded as essential for biodiesel production, as two goals can be achieved simultaneously; that is, nutrient removal efficiency and biomass production. Therefore, this study examined the effects of carbon sources, the N:P ratio, and the hydraulic retention time (HRT) to identify the optimal conditions for nutrient removal efficiency and biomass production. The effluent from a 2nd lagoon was used to cultivate microalgae. Whereas the algal species diversity and lipid content increased with a longer HRT, the algal biomass productivity decreased. Different carbon sources also affected the algal species composition. Diatoms were dominant with an increased pH when bicarbonate was supplied. However, 2% $CO_2$ gas led to a lower pH and the dominance of filamentous green algae with a much lower biomass productivity. Among the experiments, the highest chlorophyll-a concentration and lipid productivity were obtained with the addition of phosphate up to 0.5 mg/l P, since phosphorus was in short supply compared with nitrogen. The N and P removal efficiencies were also higher with a balanced N:P ratio, based on the addition of phosphate. Thus, optimizing the N:P ratio for the dominant algae could be critical in attaining higher algal growth, lipid productivity, and nutrient removal efficiency.

• 한국환경농학회지
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• 제41권3호
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• pp.158-166
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• 2022

BACKGROUND: Kiwifruit is a fruit tree with relatively small cultivation area in Korea and researches on its soil and physiology are very limited compared to those on cultivar development. Therefore, there are limited information for farmers to cope with the reduction in productivity due to various physiological disorders and premature aging. This study was conducted to investigate the soil and leaf chemical properties, and fruit characteristics, which will be used as basic data for stable kiwifruit orchard soil management. METHODS AND RESULTS: The soil and leaf chemical properties, and fruit characteristics were investigated for two years in 16 kiwifruit orchards growing 'Hayward' (Actinidia deliciosa) in Jeollanam-do and Gyeongsangnam-do. Soil and leaf samples were collected in July and fruit quality was investigated by harvesting fruits about 170 days after full bloom. The average soil chemical properties of kiwi orchards were generally higher than the recommended level, except for pH, and especially, the exchangeable potassium reached about 300% of the recommended level. The proportions of orchards that exceeded the recommended level of soil chemical properties were 63, 31, 100, 69, 94, 88 and 69% for pH, EC, organic content, available phosphate, and exchangeable potassium, calcium and magnesium, respectively. Thirty-three percent of orchards had more than 100 mg/kg of nitrate nitrogen in soil. Available phosphate in soil showed a significantly positive correlation with leaf nitrogen, phosphoric acid and calcium content, but showed a significantly negative correlation with leaf potassium content. The magnesium content in the leaves was significantly correlated with soil pH. The highest fruit weight was observed in about 25 g/kg of leaf nitrogen content which could be attained when plants were grown on the soil containing about 100 mg/kg of nitrate nitrogen content. The average soluble solids content among 16 orchards was 9.58 °Brix at harvest and 13.9 °Brix after ripening, which increased about 45%, and the average fruit weight was about 110 g. CONCLUSION(S): For fruit quality, fruit soluble solids (sugar compounds) content was significantly correlated with leaf potassium content, fruit hardiness with leaf total nitrate, calcium and magnesium, and fruit titratable acidity with leaf magnesium; however, leaf calcium and magnesium negatively affect the soluble solids contents in fruits.

• The Plant Pathology Journal
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• 제34권3호
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• pp.208-217
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• 2018

In this study, samples were collected from the leaves and stems of healthy wild Pistachio trees (Pistacia atlantica L.) from various locations of Baneh and Marivan regions, Iran. In total, 61 endophytic bacteria were isolated and grouped according to phenotypic properties. Ten selected isolates from each group were further identified by partial sequencing of the 16S rRNA gene. Based on the results, isolates were identified as bacteria belonging to Pseudomonas, Stenotrophomonas, Bacillus, Pantoea and Serratia genus. The ability of these isolates was evaluated to phytohormone production such as auxin and gibberellin, siderophore production, phosphate solubilization, atmospheric nitrogen fixation, protease and hydrogen cyanide production. All strains were able to produce the plant growth hormone auxin and gibberellin in different amounts. The majority of strains were able to solubilize phosphate. The results of atmospheric nitrogen fixation ability, protease and siderophore production were varied among strains. Only Ba66 could produce a low amount of hydrogen cyanide. The results of biocontrol assay showed that Pb78 and Sp15 strains had the highest and lowest inhibition effects on bacterial plant pathogens, Pseudomonas syringae pv. syringae Pss20 and Pseudomonas tolaasii Pt18 under in vitro condition. Pb3, Pb24 and Pb71 strains significantly promote root formation on carrot slices. To our knowledge this is the first report of the isolation of endophytic bacterial strains belonging to Pantoea, Bacillus, Pseudomonas, Serratia and Stenotrophomonas genus from wild pistachio trees with plant growth promoting potential and biocontrol activity.

• 한국약용작물학회지
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• 제22권4호
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• pp.306-312
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• 2014

Endophytes are microorganisms that live in the internal tissues of plants without harming the host plants. In this symbiotic relationship, the host plants provide nutrients and shelter to the endophytes, in turn, endophytes can promote the growth of host plants and act as a biological control agents against plant pathogens. Plant-microbe interactions like this are noted for natural methods for sustainable agriculture and environmental conservation. However, in spite of the infinite potential, there are only a few reports on the endophytes present in ginseng. In this study, we isolated and identified the endophytes from Panax ginseng seeds and evaluated the biological activities (IAA production ability, nitrogen fixation ability, phosphate solubilization capacity, siderophore production ability, and antifungal activities) of the endophyte isolates. Eight different endophytes were identified by 16S rRNA sequencing. Most of the endophytes have antibiotic and plant growth promoting (PGP) activities. Particularly, PgSEB5-37E have the highest antibiotic activity, both PgSEB5-37B and PgSEB5-37H have high PGP traits such as an abilities to produce IAA, solubilize phosphate and fix nitrogen. These results indicated that the endophytes from P. ginseng seeds may have applicable value to many industries. In order to use the isolated endophytes, quantitative analysis and field tests are needed to be performed.