• Title/Summary/Keyword: Phorbol 12-Mysistrate 13-Acetate

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Expression of the Heat Shock Proteins and Glucose-Regulated Proteins during Phorbol 12-Myristate 13-Acetate-Induced Megakaryocytic Differentiation of K562 Erythroleukemia Cells (K562 백혈구암 세포의 Phorbol 12-Myristate 13-Acetate에 의한 대핵세포로의 분화과정에서 Heat Shock Proteins와 Glucose-Regulated Proteins의 발현)

  • 이창훈;김우진;김종묵;한송이;김정락;한규형;임운기;유미애;강호성
    • The Korean Journal of Zoology
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    • v.39 no.1
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    • pp.47-53
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    • 1996

  • We examined the expression of the heat shock proteins (HSPs) and glucose-regulated proteins (GRPs) during phorbol 1 2-myristate 1 3-acetate (PMA)-induced megakaryocytic differentiation of human er"'throleukemia K562 cells. PMA-treated K562 cells showed a cell growth arrest and alteration in morphology and patterns of gpllIa and c-myc expression, characteristic of megakaryocytic differentiation. During the megakaryocytic differentiation, HSP9OA, HSP9OB, and HSP28 mRNA and protein levels markedly decreased, while GRP78/B and GRP94 mRNA levels were enhanced. On the other hand, HSP7OA and HSP7OB mRNA levels were reduced, but HSP7O protein levels were not changed by PMA treatment. These results suggest specific roles for the HSPs and GRPs in K562 cell proliferation and megakaryocic differentiation.tion.

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