• 한국균학회지
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• 제44권1호
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• pp.51-56
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• 2016

본 연구에서는 강원도의 설악산에서 주목(Taxus cuspidata Siebold & Zucc.)과 잣나무(Pinus koraiensis Siebold & Zucc.), 경기도 화악산에서 분비나무(Abies nephrolepis(Traut.) Maxim.), 경북 울릉도에서 주목 등의 침엽을 채취하여 내생균을 분리하였고, 분리된 균주의 형태학적 특징을 관찰하고, ITS rDNA와 LSU rDNA지역의 염기서열 분석하여 균을 동정하였다. 그 결과 Biscogniauxia maritime, Nemania diffusa, Pezicula carpinea, Phomopsis juglandina, Sydowia polyspora 등의 5종의 국내 미기록 내생균을 확인하였고, 이를 보고하고자 한다.

• Mycobiology
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• 제50권5호
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• pp.317-325
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• 2022

Endophytic fungi occupy various ecological niches, which reinforces their diversity. As few studies have investigated the endophytic fungi of alpine conifers, we focused on four species of alpine conifers in this study-Abies nephrolepis, Pinus pumila, Taxus cuspidata var. nana, and Thuja koraiensis-and examined them for endophytic fungi. A total of 108 endophytic fungi were isolated. There were four taxa in A. nephrolepis, 12 in P. pumila, 18 in T. cuspidata var. nana, and 17 in T. koraiensis; these were divided into five classes: Agaricomycetes (3.2%), Dothideomycetes (29.0%), Leotiomycetes (15.0%), Sordariomycetes (41.9%), and Orbiliomycetes (1.6%). The most prevalent fungi were Sydowia polyspora (22.7%) and Xylariaceae sp. (22.7%) in P. pumila, Phomopsis juglandina (16.1%) in T. cuspidate var. nana, and Thuja-endophytes sp. 1 (70.0%) in T. koraiensis. However, there was no dominant species growing in A. nephrolepis. Some host plants were analyzed using next-generation sequencing. We obtained 4618 reads for A. nephrolepis and 2268 reads for T. koraiensis. At the genus level, the top three endophytic fungi were Ophiostomataceae_uc (64.6%), Nectriaceae_uc (15.5%), and unclassified organism (18.0%) in A. nephrolepis and Nectriaceae_uc (41.9%), Ophiostomataceae_uc (41.8%), and Magnaporthaceae_uc (9.2%) in T. koraiensis. Our results show that there are different communities of endophytic fungi among different host plants, even if the host plants are in the same region. Such ecological niches are important in terms of the ecological restoration of alpine conifers.