• Title/Summary/Keyword: Pho

검색결과 86건 처리시간 0.027초

중국 단령명칭에 관한 연구 (A study on the name of Dan-Ryong in China)

  • 문광희
    • 한국의류학회지
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    • 제11권1호
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    • pp.87-96
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    • 1987
  • Dan-Ryong is the traditional clothing that was worn the entire of oriental nations, and it was spreaded from Sun-Bi tribe to China about A.D. 4C. The first, the name of Dan-Ryong was translated with 25-Sa and Ancient-History Book. Among the name of Dan-Ryong, the follows were different between the name and shape. Jang-Bok was the colored Dan-Ryong, So-Bok was the unfigured black Dan-Ryong and removed Hyung-Bae, Ea-Mun-Pho was the Dan-Ryong of embroidered figure poetry, and Ja-Sam was the real short and tight Dan-Ryong. The second, some problems were given by translated Dan-Ryong's name. The results of the problems were as follows; 1. Dan-Ryong was oriented from Won-Wi. 2. At Su-Dynasty, there was going to the persuit of Han-Dynasty Courtesy, but they liked useful custom. Therefore Dan-Ryong was worn in daily life among the population. 3. At Dang-Dynasty, Ho-Bok was devided with three meanings. The first was Go-Sub, the second was Dan-Ryong of Buk-Jo about A.D. 3$\~$5 C and the last meaning was the clothing of Uighur, Turkey, Persia and etc. about A.D. 7 C. 4. The name of neck-line shape was started at Song and the majority arised about A.D. 12 C. and Dan-Ryong was only arised at Myong-Dynasty. 5. Gok-Ryong, except Song-Sa and Sam-Je-He-Bo, was different from Dan-Ryong and it was shape of neck-line inside of Jik-Ryong.

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Rhodospirillum rubrum Plasmid pKY1의 유전정보 분석과 그의 활용에 관한 연구 (Genetic Analysis and its Application of Rhodosprillum rubrum PKY1 Plasmid)

  • 김복환;김정목
    • 미생물학회지
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    • 제40권2호
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    • pp.172-177
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    • 2004
  • 광합성 박테리아 Rhodospirillum rubrum은 광원의 조건에 따라 균주 내의 신진대사 양상이 변화된다고 보고 되어 왔다. 이러한 광조건에 의한 신진대사 변환 과정을 담당하는 유전자에 대한 연구는 광합성 박테리아에 관한 주된 과제 중 하나이다. 근래 이러한 광조건에 의한 균주내 변화 과정이 균주가 지니는 extrachromosomal plasmid와 관련이 있음이 보고되었다. 본 연구는 광합성 박테리아 R. rubrum의 extrachromosomal plamid pKYl 을 분리 정제하고 이를 제한효소 HindIII로 단편화 하여 이들 단편의 일부에 대한 염기서열을 분석하고 이들의 기능을 추론하였다. 본 연구를 통하여 plasmid pKY1에 박테리아의 유전적 재조합 (genetic recombination)에 관여하는 단백질에 대한 정보가 위치하고 있음을 유전자 및 아미노산 상동성 조사를 통하여 알 수 있었다.

효모, Saccharomyces cervisiae의 GAP 유전자를 이용한 발현 벡터계의 개발 (Construction of an Expression Vector System with the GAP Promoter in Saccharomyces cerevisiae)

  • 황요일;서애란;심상국;정동효
    • 한국미생물·생명공학회지
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    • 제19권6호
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    • pp.568-574
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    • 1991
  • 효모에서 대랑의 물질생산계를 구축하기 위하여 먼저 여러 종류의 베터의 이용이 가능한 다양한 영양요구성 marker를 지니며 형질전환율이 향상된 효모숙주를 선별 개량하였다. 벡터의 제작에 사용되는 프로모터로는, 효모의 여러 유전자 중에서 그 활성이 매우 높은 해당계의 효소 GAP-DH의 구조 유전자 GAP를 이용하기로 하여, 효모염색체 DNA중에서 GAP 프로모터를 분리하여 이용하기 쉽게 변형하였다. 분리된 GAT promoter의 기능을 검토하기 위하여, reporter로 APase의 구조유전자 PHO5'를 이용하여 세포내의 copy수가 상이한 발현 벡터를 제작하여 GAP 프로모터에 의한 APase의 활성 및 전사산물을 측정한 결과, 정상적인 전사가 이루어 졌으며, 효소활성도 높게 나타났으며, 벡터의 copy수에 의한 효소활성의 차이도 감지되었다.

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필댐 보수보강후 시간경과에 따른 효과 분석 (Effect analysis by time passage after Repair & Reinforcement of Fill Dams)

  • 김재홍;오병현;임은상;홍원표
    • 한국지반공학회:학술대회논문집
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    • 한국지반공학회 2008년도 춘계 학술발표회 초청강연 및 논문집
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    • pp.697-703
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    • 2008
  • Excessive water leakage phenomenon happens through damage of nation core zone of about 17,000 storage of water facilities or collapse of dam is worried, is being damaged or enforce dilapidated fill dam core zone's repair reinforcement. Example that use grouting method of construction considering construction and economic performance etc. recently by repair reinforcement way about defect of dam is increased. Permeation grouting method repair & reinforcement of fill dam countermeasure is preferred in nation. Do that is economical to decide these repair reinforcement effect and grouting effect estimation method that do not give damage to dam is effective. Therefore, observing electricity resistivity Survey change of dam since grouting reinforcement using Electric resistivity Survey inquiry of seismic survey method in this research, Wished to verify grouting effect whether is possible as Electric resistivity Survey, and study whether integrity of dam through repair reinforcement defined.

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풍화된 변성암 사면의 안정대책 사례 (Case of slope stability in weathered metamorphic rock)

  • 김재홍;박철숙;전제성;홍원표
    • 한국지반공학회:학술대회논문집
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    • 한국지반공학회 2008년도 춘계 학술발표회 초청강연 및 논문집
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    • pp.1400-1405
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    • 2008
  • Execute surface of the earth geological survey for stability analysis of stealing cutting sides and investigated wide area nature of soil structure. Inflected DIPS that measure discontinuity's direction that develop to slope and is slope stability a wide use program for Stereographic Projection Method analysis that utilize geometrical correlation of stealing four sides and discontinuity surface. It is principle that angle of internal friction that is basis element of stability estimation applies direct shear test result on joint side, Examination is impossible by case execution, suppose by 30 angles that apply more conservatively in base rock slope sides usually and achieved analysis. When analyze, consider discontinuity's various adult that develop in research slope, after conduct first each discontinuity different assay falling into fault, joint, executed stability estimation which synthesize whole discontinuity data. When ailment element is recognized as analysis result, wished to present stability countermeasure way of most suitable to take into account of execution, stability, economic performance.

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PBK/TOPK Expression During TPA-Induced HL-60 Leukemic Cell Differentiation

  • Liu, Yu-Hong;Gao, Xue-Mei;Ge, Fan-Mei;Wang, Zhe;Wang, Wen-Qing;Li, Xiao-Yong
    • Asian Pacific Journal of Cancer Prevention
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    • 제13권5호
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    • pp.2145-2148
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    • 2012
  • Objective: This study concerns expression of PBK/TOPK during differentiation of HL-60 leukemic cells induced by tetradecanoyl phorbol acetate (TPA). Methods: Wright-Giemsa staining was performed to observe morphological changes in the HL-60 cells, and flow cytometry was used to assess the cell cycle and CD11b, CD14, CD13, and CD33 expression. PBK/TOPK levels were determined by Western blot analysis. Results: After treating HL60 cells with $5.1{\times}10^{-9}$ mmol/L of TPA for three days, the number of nitroblue-tetrazolium-positive cells and CD11b, CD13, and CD14 expression increased, whereas the PBK/TOPK levels decreased. Conclusions: TPA can inhibit proliferation and induce differentiation of HL60 cells of the granulocytic or monocytic lineage. PBK/TOPK expression was downregulated during this process, whereas the Pho-PBK/TOPK expression was increased.

Characterization of C-P Lyase gene cluster by in vivo $^{31}$ P-NMR spectroscopy

  • Lee, Ki-Sung;Kwak, In-Young
    • Journal of Microbiology
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    • 제33권4호
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    • pp.328-333
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    • 1995
  • $\^$31/ P-NMR experiment was performed to detect phophonates (Pn) utilization and degradation in the several different C-P lyase mutants of E. coli and in E. aerogenes and the recombinants. The relative peak intensity (RPI) for the standard samples of 0.5 mM methylphosphonate (MPn) and 1.0 mM aminoethylphosphonate in glucose-MOPS medium showed 0.5 : 1.0 ratio. In the case of BW14329 (.DELTA.phnC-P, .delta.phoA), RPI did not change significantly after 24 hrs culturing, which means it nearly could not utilize Pn. In vivo $\^$31/ P-NMR spectrum of E. aerogens (BWKL 16627) during 3 hrs starvation showed two intense peaks at 0-2 ppm and at near-10 ppm which indicate intracellular orthophosphate (Pi) and pyrophosphate (PPi), respectively. Both of them might be released by degradation of inorganic polyphosphate pool. When MPn is supplied to the medium as an unique P source, Pi content in the cell has the constant, but PPi seems to be slightly decreased. Recombinants (BWKL 16954) grew slower than E. aerogenes in the glucose-MOPS media with various P sources. In vivo $\^$31/ P-NMR spectrum of recombinant did not show any intense signal in the cell. Surprisingly, under the cultivation adding with MPn, a few intense peaks in the region of Pi AND phospate monoester were detected.

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실리콘 웨이퍼위에 증착된 실리케이트 산화막의 CMP 슬러리 오염 특성 (CMP Slurry Induction Properties of Silicate Oxides Deposited on Silicon Wafer)

  • 김상용;서용진;이우선;장의구
    • 한국전기전자재료학회논문지
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    • 제13권2호
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    • pp.131-136
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    • 2000
  • We have investigated the slurry induced metallic contaminations of undoped and doped silicate oxides surface on CMP cleaning process. The metallic contaminations by CMP slurry were evaluated in four different oxide films, such as plasma enhanced tetra-ethyl-orthyo-silicate glass(PE-TEOS), O3 boro-phos-pho-silicate glass(O3-BPSG), PE-BPSG, and phospho-silicate glass(PSG). All films were polished with KOH-based slurry prior to entering the post-CMP cleaner. The Total X-Ray fluorescence(TXRF) measurements showed that all oxide surfaces are heavily contaminated by potassium and calcium during polishing which is due to a CMP slurry. The polished O3-BPSG films presented higher potassium and calcium contaminations compared to PE-TEOS because of a mobile ions gettering ability of phosphorus. For PSG oxides, the slurry induced mobile ion contamination increased with an increase of phosphorus contents. In addition, the polishing removal rate of PSG oxides had a linear relationship as a function of phosphorus contents.

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폴리락트산/폴리카프로락톤 블렌드의 결정화 거동 (Crystallization Behavior of Poly(lactic acid) / Poly($\varepsilon$-caprolactone) Blends)

  • 이종록;천상욱;강호종
    • 폴리머
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    • 제27권4호
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    • pp.285-292
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    • 2003
  • 폴리락트산/폴리카프로락톤 (PLA/PCL) 블렌드의 조성비에 따른 상용성 및 결정화 거동을 살펴보았다. 특히 triphenyl phosphite (TPP)가 반응 상용화제로써 이들 블렌드의 상용성과 결정화 거동에 미치는 영향을 중점적으로 고찰하였다. PLA/PCL 블렌드의 열적 특성을 살펴보아 이들 블렌드가 비상용성 블렌드임을 확인하였으며 블렌드의 조성비에 따라 상용성 정도가 달라졌다. 블렌드에 TPP를 첨가하는 경우 TPP가 반응 상용화제로 작용하여 블렌드의 상용성을 증가시키며 상용화제 함량 증가에 따라 상용성이 우수해졌다. PLA에 PCL을 첨가하는 경우, 블렌드 조성비에 따른 비상용 특성에 의한 계면 형성이 구정 형성에 필요한 기핵 발현 정도를 변화시켜 결정화 속도가 달라졌다. TPP는 이들 블렌드의 상용성의 개선과 동시에 구정 성장을 촉진하여 PLA의 결정화 속도를 증가시킴을 확인하였다.

진핵세포 유전자의 기초대사 발현을 조절하는 trans 작용인자의 기능해석과 새로운 인자의 분리 (Elucidation of Function and Isolation of Trans-acting Factors Regulating the Basal Level Expression of Eukaryotic Genes)

  • 황용일
    • 한국미생물·생명공학회지
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    • 제19권1호
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    • pp.37-44
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    • 1991
  • 진핵세포 유전자의 기초대사발현의 조절계를 밝히기 위한 일환으로, 효모의 histidine생합성계 효소의 구조유전자 HIS5를 이용하였다. HIS5 유전자는 충분한 아미노산 조건하에서는 발현이 억제되어 비교적 높은 기초발현만을 하나, 어떤 아미노산이 결핍되면 탈억제되어 높은 발현량을 보이며 탈억제는 cis의 작용인자인 promoter상의 5'-TGACTC-3' 및 trans 작용인자 GCN4와 GCD17 GCN2등이 관여한다. trans 작용인자들에 의한 HIS5 유전자의 발현량의 변화를 간단하게 측정하기 위하여, HIS5 promoter와 repressible acid phoshates(APase)의 구조유전자중 promoter를 제거한 DNA단편을 연결시켜 HIS5-PHO5 융합유전자를 이용하였다. gcn2 및 gcn4 변이주의 APase 활성은 야생주와 비교하여 3내지 4배 낮았으며, gcn2변이주와 gcn2 gcn4 이중변이주의 APase 활성은 유사하였다.

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