• 한국자원식물학회:학술대회논문집
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• 한국자원식물학회 2018년도 추계학술대회
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• pp.132-132
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• 2018

The aim of this study was to enhance the Tartary buckwheat growth and phenolic profile by the application of $SiO_2$ on potsin the glasshouse. The liquid of $SiO_2$ were applied at three times after three weeks of sowing at ten days interval. The doses of the $SiO_2$ was low (2.5 ml/5 L water), high (10 ml in 5 L water) and control experiment was done without $SiO_2$. In this study, it is clearly shown that high dose of $SiO_2$ increased the buckwheat plant growth including, plant height, leaf length and width, stem diameter, fresh weight and number of seed per plant compared to control treatment. In the same way, the total phenolic compound (1421 mg/100 g), total flavonoid (35.1 mg/100g), rutin (3,130 mg/100g) and DPPH (82%) in plant were also increased high dose of $SiO_2$ compared to control (1,175 mg/100g, 31.9mg/100g, 860 mg/100g, 59%, respectively). Moreover, higher phenolic compound (1,421 mg/100g) and DPPH (82%) was observed in plant compared to seed (196 mg/100mg, 72 %, respectively). In the contrary, total flavonoid (36.2 mg/100g) and rutin (1,400 mg/100g) was higher in buckwheat seed compared to plant (35.1 mg/100g, 3,130 mg/100g, respectively). Finally, it is concluded that higher dose of $SiO_2$ enhance buckwheat growth and phenolic profile. Further investigation is needed to evaluate the optimum dose of $SiO_2$ according to soil conditions in the field.

• 한국작물학회:학술대회논문집
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• 한국작물학회 2017년도 9th Asian Crop Science Association conference
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• pp.290-290
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• 2017

Rice (Oryza sativa L.) is one of the most consumed staple food crop which is energy source as carbohydrate and also is considered as the important antioxidant sources including various phenolic compounds. According to the increasing demand of healthy life, the concern to antioxidant also is increasing because of its health-promoting effect. Phenolic compounds are one of the plant secondary metabolites class, which shows various benefits to preventing or treating chronic diseases. In this study, we have measured the total phenol content from total 647 rice samples using the Floin-Ciocalteau method, and then were selected 30 rice genetic resources classified with high, middle, and low group on the basis of total phenol content. The average of the total phenol content of each group was high-group ($6892.9{\pm}488.5{\mu}g\;GAE/g$) > middle-group ($1428.1{\pm}76.0{\mu}g\;GAE/g$) > low-group ($97.6{\pm}11.4{\mu}g\;GAE/g$). The selected rice samples were analyzed with LC-MS/MS to find the composition and concentration of individual phenolic in rice grain. High-group and middle-group contained large amounts of protocatechuic acid and (+)-catechin whereas low-group showed limited amount. Among high-group samples, rice samples with black pericarp color (IT 174089, IT 220079, and IT 259958) had high content of peonidin-3-O-glucoside. Further, these black rice samples were special since polydatin, rarely found stilbenoid in rice grain, was detected. Overall, both the sum of phenolic acid and the sum of flavonoid were high-group > middle-group > low-group. Also, each group exhibited different phenolic compositions; high-group consisted of flavonoid more than phenolic acid, middle-group and low-group was comprised of phenolic acid rather than flavonoid, and non-pigmented rice was composed by fully phenolic acid. The total phenol content had positive relationships with the sum of phenolic compound (r = 0.64), the sum of flavonoid (r = 0.74) at the significance level of p < 0.0001. In addition, protocatechuic acid and quercetin showed positive correlation with above phenolic composition parameters; in order, r = 0.98, 0.65 for protocatechuic acid and r = 0.73, 0.78 for quercetin (p < 0.0001). In conclusion, the total phenol content assay showed the possibility of utilization as a phenolic composition indicator in rice grain. Also, this result was suggested study pigment on other material.

• 임산에너지
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• 제22권3호
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• pp.11-19
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• 2003

아까시나무에 있어서 알레로파시 효과를 분석하기 위해 아까시나무를 포함하여 13개 수 종에 대한 종자발아 시험을 실시하였다. 아까시나무의 뿌리와 잎의 침출액을 실험에 사용하였다. 침출액에 대한 종자 발아실험 결과 수종에 따라 실험결과가 크게 다르게 나타났다. 아까시나무 잎 침출액에서 아까시나무 종자 발아는 30%가 억제되었다. 실험한 수종가운데 무궁화와 오리나무는 뿌리 침출액에서 억제되었으나 누운측백나무는 잎의 침출액에서 발아가 억제되었다. 아까시나무의 잎과 뿌리 침출액을 HPLC분석 한 결과 알레로파시 효과를 가지고 있는 8종류의 authentic phenolic compounds를 찾아냈다. 잎에서는 전체 페놀물질 중 40%를 차지하고 있는 gentisic acid를 뿌리에서는 syringic acid와 vanillicacid를 발견하였다. 전체적으로 볼 때 뿌리에서.보다 잎에서 7배가 많은 phenolic acids가 추출되었다 조사된 8개 phenolic acid 중에서 뿌리에서는 vanillin만이 잎에서는 coumaricacid 만이 발견 되었다. 결과적으로 13수종 중에 3개 수종에서 잎과 뿌리의 침출액에서 종자 발아가 억제되었다. 종자발아 시험결과 아까시나무의 화학물질의 상호작용은 수종에 따라 차이가 많이 있음을 밝혀냈다. 아까시나무의 phenolic compound 총량은 알레로파시 역할에 큰 영향을 미치지 않았으나 이들 물길의 상승효과가 주요한 역할을 하는 것으로 추정 하였다.

• 대한화장품학회지
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• 제27권1호
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• pp.99-109
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• 2001

Novel glucoconjugates phenolic moiety, 3-(methoxycarbonyl)-4-(hydroxyphenyl)-$\beta$-D-glucopyranoside(4), 3-(methoxyacetyl)-4-(hydroxyphenyl)-$\beta$-D-glucopyranoside(7), 4-(hydroxyphenyl)-$\beta$-D-ribofuranoside(11), were synthesized. In order to investigate their depigmentation effect, inhibitory activity against mushroom tyrosinase and inhibitory activity of melanin synthesis in B16 melanoma cell were evaluated in vitro. Compound 11 showed 92.0$\mu\textrm{g}$/㎖ of tyrosinase inhibitory activity whereas compound 4 and 7 showed very low activity not less than 300$\mu\textrm{g}$/㎖. Inhibitory activities of melanin synthesis in B16 melanoma cell of compound 4, 7, and 11 were 8.7, 15.1, and 36.0%, respectively, at the concentration of 100$\mu\textrm{g}$/㎖. Inhibitory activity of compound 11 was much higher than that of arbutin at the same concentration.

• Natural Product Sciences
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• 제6권2호
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• pp.82-85
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• 2000

The aqueous ethanolic whole plant extract of Rosa arabica was found to contain the new natural dimeric phenolic compound, ellagic acid 3,3'-dimethyl ether $4-O-{\alpha}-rhamnopyranoside$, 9, along with ten known phenolic metabolites (1-8, 10 and 11). Structures of all compounds (1-11) were established by routine methods of analysis and confirmed by FAB-MS, $^1H\;and\;^{13}C$ NMR spectral analysis.

• 한국식물학회:학술대회논문집
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• 한국식물학회 1989년도 심포지움 및 웍샵 식물과 토양 Plant and Soil
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• pp.101-109
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• 1989

• 한국자원식물학회지
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• 제23권5호
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• pp.451-457
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• 2010

참외의 비식용부위인 씨, 꼭지, 줄기 잎 부위의 항산화 기능성을 구명하기 위하여 DPPH, ABTS, FRAP, SOD 등 다양한 항산화 실험법을 이용하여 항산화 활성을 평가하였다. 그 결과 참외 비식용부위의 항산화 활성은 참외 꼭지부위에서 가장 높은 항산화 활성을 보여주었으며, 농도 의존적으로 활성이 증가하였다. 총 페놀 성분 또한 꼭지 생체 100 g당 143.4 mg으로 가장 높게 나타났다. 항산화 활성과 총 페놀간의 상관관계를 조사한 결과 높은 상관관계가 있음을 확인할 수 있었다. 위의 결과를 종합하면 참외의 비식용부위 중 꼭지에서 항산화 활성과 총 페놀 함량이 가장 높게 나타났다. 따라서 예로부터 약용으로 사용되고 있는 참외 꼭지에서 항산화 활성 및 기능성분 함량이 높게 나타남으로써 향후 다양한 생리활성 및 활성성분 규명 등에 대한 지속적인 연구가 필요할 것으로 사료된다.

• 한국약용작물학회지
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• 제15권2호
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• pp.120-127
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• 2007

The objective of this research was to evaluate the ability of water and ethanol extracts from mulberry fruit (Morus alba L.) to influence the inhibitory activity of angiotensin converting enzyme (ACE) and xanthine oxidase(XOase). The total phenol contents and sixteen phenolic compounds were investigated in water and ethanol extracts. In order to understand the factors responsible for the potent antioxidant and antihypertensive ability of mulberry, it has been evaluated for anti-oxidative activity using Fenton's reagent/ethyl linoleate system and for free radical scavenging activity using the 1,1-diphenyl-2-picryl hydrazyl free radical generating system. The total phenol contents and total of phenolic compounds in ethanol extract showed higher levels than water extract in mulberry fruit six phenolic compounds (chlorogenic acid, narigin, syringic acid, quercetin, naringenin, kampferol) has a higher individual phenolic compound content in the 60% ethanol extraction than 80% ethanol extract. The inhibitory activity on angiotensin converting enzyme (ACE) were highest in 80% ethanol extract (9.0%). Also, activity of xanthine oxidase(XOase) inhibition appeared highest in 80% ethanol extracts and correlated well with the total phenolic content, which was modulated by the concentration of individual phenolic compounds. This result revealed, that strong biological activity was caused by specific phenol compound contents. Utilization of water and ethanol extracts from mulberry fruit are expected to be good candidate for development into source of free radical scavengers and anti-hypertentive activity

• 한방비만학회지
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• 제23권1호
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• pp.10-17
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• 2023

Objectives: The aim of this study was to compare the total phenolic content of Korean Acanthopanax senticosus stems harvested in different seasons and provide basic data for developing functional reinforcement products based on the optimal harvesting time. Methods: Each sample harvested in different seasons was extracted and concentrated twice for 2 hours using 70% ethyl alcohol. Phenolic compounds were analyzed using high-performance liquid chromatography for simultaneous multi-component analysis of 14 compounds, including syringaresinol and so on. Results: The results showed that the stem of Korean Acanthopanax senticosus harvested in winter (November 29th) (EAS-5) had the highest phenolic content of 1.038%. The stem of Korean Acanthopanax senticosus harvested in autumn (October 1st) (EAS-4) showed the second-highest phenolic content of 0.764%, followed by the stem of Korean Acanthopanax senticosus harvested in spring (February 2nd) (EAS-1) with a content of 0.390%. On the other hand, the stem of Korean Acanthopanax senticosus harvested in the summer (June 2nd) (EAS-3) showed the lowest content at 0.342%. In conclusion, the stem of Korean Acanthopanax senticosus harvested in winter (EAS-5) showed the highest phenolic compound content. Conclusions: Considering the extraction yield and the total phenolic content, as well as the concentrations of key functional components such as eleutheroside B, chlorogenic acid, and syringaresinol in the 70% ethyl alcohol extract of Korean Acanthopanax senticosus, it is suggested that the stems of Korean Acanthopanax senticosus harvested during the winter season are suitable for the development of novel materials with enhanced anti-obesity functionality.

• 한국식품과학회지
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• 제52권3호
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• pp.205-211
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• 2020

국내산 포도 품종 'Campbell Early', 'Kyoho', 'Heukbosuk', 'Hongju Seedless (SDS)'와 수입 포도 'Crimson SDS', 'Thomson SDS'의 페놀계 화합물을 비교하기 위해 LC-MS/MS와 HPLC를 이용하여 함량을 비교하였다. 수입포도 'Crimson SDS'와 'Thompson SDS' 품종은 국내산 포도 품종보다 가벼운 과피무게(300-350 mg)와 단단한 경도(5.2-5.6 kg·f)를 가진 것으로 조사되었다. 'Crimson SDS' 품종의 과피에 함유된 페놀계 화합물 함량은 다른 포도 품종에 비해 전반적으로 높았지만, resveratrol (25-29 mg/kg), quercetin (350-380 mg/kg), myricetin (31-32 mg/kg) 함량은 'Hongju SDS' 품종과 유사하였다. Anthocyanin 함량은 포도 품종에 따라 달랐는데, 'Hongju SDS' 품종은 Delphinidin-3-glucoside의 함량이 높았고, 'Crimson SDS' 품종은 Peonidin-3-glucoside의 함량이 높았다. 페놀 화합물의 함량은 포도 부위별 다른 함량을 나타냈고, catechin, epicatechin, procyanidin B1, B2는 포도 종자에서 전형적인 화합물이었다. 과피를 함께 먹을 수 있는 포도품종은 과피에 포함된 유용한 phenolic compounds를 효과적으로 이용할 수 있다.