• 한국균학회지
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• 제26권4호통권87호
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• pp.466-477
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• 1998

진흙버섯류 22개 균주를 균총의 형태와 PCR 기법을 사용하여 종간의 구분 방법을 찾고자 하였다. PDA등 4가지 배지에서 균사생장 및 배지의 변색여부 등을 기준으로 특성을 구분할 때 목질진흙버섯의 균총 색깔은 진한 황색으로 균사생장이 늦고 배지를 푸르게 변색시켰다. rDNA 분석 결과 $ITSI{\sim}II$ 부위는 목질진흙버섯이 약 800 bp, 말똥진흙버섯은 약 700 bp였고, IGRI 부위는 목질진흙버섯은 약 700 bp, 말똥진흙버섯은 균주에 따라 약 500, 600, 700, 800 bp에서 4가지 각기 다른 밴드를 보였다. $ITSI{\sim}II$와 IGRI부위의 증폭된 DNA를 6개의 제한효소로 절단하여 다형성을 비교해 본 결과 $ITSI{\sim}II$의 HaeIII 절단으로 목질진흙버섯과 말똥진흙버섯을 구분할 수 있었으며 이들 밴드를 이용하여 유연관계를 조사한 결과 목질진흙버섯은 95%의 유사도를 보였으며, 말똥진흙버섯은 89%의 유사도로 complex를 형성하였다. 목질 진흙버섯은 RAPD 분석과 AP-PCR에 의한 밴드양상으로도 확실한 구분이 가능하였으며, $ITSI{\sim}II$ 부위의 HaeIII 제한효소 처리로 나타난 벤드는 이종의 특이적인 marker로 사용할 수 있을 것으로 본다.

• 농업과학연구
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• 제35권2호
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• pp.147-153
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• 2008

본 시험은 상황버섯박의 사료가치를 조사하기 위하여 화학적 성분을 분석하고, 산란계를 이용하여 급여수준(급여사료의 0, 5, 10 및 20%)에 따른 대사율과 혈청 IgG 농도를 조사하였다. 영양적 조성에 있어서는 섬유질성 단미사료 수준 이상의 영양소를 함유하고 있으며, 산란계에 대한 대사율은 대조구에 비하여 상황버섯박 10 및 20% 첨가구가 유의하게 낮은 결과를 보였다(p<0.05). 그리고 혈청 IgG 농도에 있어서는 첨가수준에 따른 유의적인 차이를 발견할 수 없었다. 위 결과로 볼 때 상황버섯박은 섬유질 함량이 높아 단위 동물의 사료로는 제한적 사용이 불가피하며, 버섯의 약리성분은 추출잔류물이라는 특성으로 볼 때 그 작용을 기대하기는 어려울 것으로 판단된다. 또한 상황버섯박은 섬유질 함량이 높은 것으로 보아 단위 동물보다는 반추동물의 사료로 이용하는 것이 유리할 것으로 생각된다.

• Mycobiology
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• 제30권2호
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• pp.82-87
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• 2002

Species of Phellinus were known to harmful fungi causing white pocket rot and severe plant disease such as canker or heartrot in living trees in the West, but some species have been used to traditional medicines in the Orient for a long time. In this study the partial D1-D2 nucleotide sequences of 28S ribosomal DNA from 13 Phellinus strains were determined and compared with the sequences of 21 strains obtained from GenBank database. According to the neighbor-joining(NJ) method comparing the sequence data the phylogenetic tree was constructed. The phylogenetic tree displayed the presence of four groups. Group I includes P. ferreus, P. gilvus and P. johnsonianus, Group II contains P. laevigatus, P. conchatus and P. tremulae, Group III possesses P. linteus, P. weirianus, P. baumii, P. rhabarbarinus and P. igniarius, and Group IV comprises P. pini, P. chrysoloma. P. linteus and P. baumii, which were used mainly in traditional medicine, belong to the same group, but exactly speaking both were split into two different subgroups. To detect P. linteus only, we developed the PCR primer, D12HR. The primer showed the specific amplification of P linteus, which is permitted to medicinal mushroom in the East. The results make a potential to be incorporated in a PCR identification system that could be used for the rapid identification of this species from its related species, P. linteus especially.

• 한국균학회지
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• 제41권4호
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• pp.243-247
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• 2013

최근 농가에서 재배하고 있는 상황버섯 종에 비하여 균사생장이 빠르고 자실체 발생과 생육이 빠른 P. linteus HN1009K (HK1009)가 개발되었다. 본 연구는 HK1009의 균사체와 자실체 에탄올 추출물의 항산화 및 항염증효과를 기존에 재배되고 있는 P. linteus (PL, Korea Sangwhang)과 P. baumii (PB, Jangsu Sanghwang)를 대조구로 하여 비교분석 하였다. HK1009는 균사체와 자실체 추출물 모두 PL과 PB에 비하여 항산화 활성이 높았으며 xanthine oxidase분석에서는 PL, HK1009, PB순으로 항산화 활성이 높게 나타났다. RAW264.7세포를 이용한 상황버섯 균사체 추출물 PB(EX), PL(EX), HK1009(EX)시료의 NO assay 결과는 PB, PL, HK1009 모두 농도 의존적으로 원액 $200{\mu}g/ml$ 에서는 $7{\sim}8{\mu}M$ 정도로 염증 억제 효과가 뛰어났다. RAW 264.7 세포에 대한 MTT assay 결과에서 PB(EX), PL(EX), HK1009(EX) 추출물의 원액처리구 에서도 세포의 생존능이 70% 이상을 유지하여 세포 독성이 없는 것으로 판단되었다.

• Mycobiology
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• 제36권2호
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• pp.81-87
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• 2008

The optimal conditions for mycelial growth of Phellinus linteus ATCC 26710 were determined to be a log length of 20 cm, temperature of $30^{\circ}C$ and pH of 6.0. Mycelial growth was excellent on the mushroom complete medium, and was optimal when sucrose, man nose and glucose were supplied as carbon sources. Potassium nitrate and sodium nitrate as nitrogen sources supported good mycelial growth. To evaluate P. linteus mycelial colonization on logs, sterilized short log inoculation, drilling inoculation and log-end sandwich inoculation techniques were used. Only sterilized short log inoculation produced good mycelial colonization. Initial mycelial growth and full mycelial colonization were best on 20 cm logs having 42% moisture content. The initial mycelial growth of P. linteus was accelerated over 12hr of sterilization. Basidiocarp formation was optimal using a burying method of logs after $5{\sim}6$ months, and fruiting body formation was superior in cultivation house conditions of $31{\sim}35^{\circ}C$ and in excess of 96% relative humidity.

• IMMUNE NETWORK
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• 제14권2호
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• pp.107-115
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• 2014

Phellinus linteus has been used as a traditional herbal medicine in Asian countries and is known to have anti-tumor, immunomodulatory, anti-inflammatory, and anti-allergic activities. However, the protective effects of P. linteus against experimental asthma have not been fully investigated. The objective of this study was to determine whether P. linteus ethanol extract (PLE) suppresses inflammatory response in an OVA-induced asthma model. As expected, the oral administration of PLE significantly inhibited eosinophilic airway inflammation and airway hyperresponsiveness in OVA-challenged BALB/c mice. Supporting these data, the augmentation of Th2 cytokines (IL-4, IL-5, and IL-13), eotaxin, and adhesion molecules in lung tissues and bronchoalveolar lavage fluid after OVA inhalation was markedly attenuated by PLE. Furthermore, PLE reduced OVA-induced activation of NF-${\kappa}B$ and p38 MAPK in lung tissues. Therefore, our results suggest the potential of P. linteus as a therapeutic agent for asthma.

• Mycobiology
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• 제30권4호
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• pp.197-201
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• 2002

Specific primer sets based on ribosomal DNA(rDNA) internal transcribed specer(ITS) sequences were designed for rapid detection of Phellinus linteus and P. baumii. Polymerase chain reaction(PCR) with these primers produced unique bands for each Phellinus species. The annealing temperature range is from $40^{\circ}C\;to\;55^{\circ}C$. The length of PCR products(P. linteus and P. baumii) using designed combinative primer sets of PL1F, PL2R, PB1F, PB2R, ITS5F and ITS4R, were from 520 by to 730 bp. Fifteen strains of Phellinus species including P. linteus, P. baumii, P. weirianus, P. johnsonianus, P. rhabarberinus, P. pini, P. gilvus, P. igniarius, P. nigricans and P. laevigatus were examined in this study. Five strains, including two isolated strains of P. linteus(MPNU 7001 and MPNU 7002), and two isolated strains of P. baumii(MPNU 7004 and MPNU 7005) were shown to have about 520 bp (PL1F-PL2R), 700 bp (TTS5F-PL2R) and 600 bp (PB1F-ITS4R) -sized PCR single bands respectively. This molecular genetic technique provided a useful method for rapid detection and identification of P. linteus and P. baumii.

• 한국버섯학회지
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• 제14권3호
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• pp.75-80
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• 2016

국내에서 분리한 신규 P linteus KACC93057P의 자실체의 형태적 특성을 육안적 으로 관찰한 결과 대가없이 기주부착형으로 자실체표면은 단단한 목질형태 (hardy woody) 로 동심원으로 윤층대 (zonate)와 홈을 형성하고 담갈색을 띠고 있었다. 자실체 뒷면 테두리는 진황색, 내부는 담황갈색을 띠며 현미경 미세구조를 관찰한 결과 관공은 원형또는 각진형이며, 관공구는 5-7 per mm크기로 나타났다. hyphal system은 2분지형이며 포자는 $4.5-6{\times}4-5{\mu}m$크기로 난형또는 유구형 담갈색을 띠어 P linteus와 유사한 특성을 보였다. YGMA배지상에서 KACC93057P는 $25-30^{\circ}C$가 최적온도로 균사체 성장률이 장수상황과 고려상황에 비하여 10-25% 높았으며 균사밀도가 다른 균주보다 현저히 높게 나타났다. ITS-rDNA염기서열비교결과 96% 이상의 상동성으로 I. lonicericola, I. linteus, I. baumii와 근연관계를 형성 하였다.

• 한국잠사곤충학회지
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• 제44권2호
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• pp.74-81
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• 2002

The optimal conditions for mycelial growth of P. linteus ASI 26011 were 25-30$^{\circ}C$ and pH 6.0, respectively. The mycelial growth of P. linteus was excellent on MCM medium. In case of carbon sources, the mycelial growth of P. linteus was best on the culture media that were contained with sucrose, mannose and glucose. Potassium nitrate and sodium nitrate were good for the mycelial growth of P. linteus as a nitrogen source. For comparison of the mycelial colonization of P. linteus on logs, several techniques of inoculation were tested; the sterilized short log inoculation, drilling inoculation and log-end sandwich inoculation. The mycelial colonization of P. linteus on logs was good in the treatment of sterilized short log inoculation, but poor in the traditional methods such as drilling inoculation and log-end sandwich. The initial mycelial growth and the full mycelial colonization of P. linteus were the best on 20 cm logs under the condition of 42% of moisture content in log. Also the initial mycelial growth of P. linteus was accelerated over 12 hours of sterilization. Burying method of logs after 5-6 months of incubation was the best for formation of basidiocarp of P. linteus. The formation of fruiting body of P. linteus was quite good in the cultivation house at the 31-35$^{\circ}C$ and over 96% of relative humidity.

• 대한본초학회지
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• 제23권1호
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• pp.103-108
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• 2008

Objective: Atopic dermatitis is the most common chronic inflammatory skin disease in childhood. Phellinus linteus, an orange color mushroom, has been used as a traditional herb medicine for years. Recent preliminary animal experiments suggest that Phellinus linteus grown on germinated brown rice is effective in chronic inflammatory disease. The aim of this study was to evaluate the efficacy of Phellinus linteus grown on germinated brown rice in the treatment of atopic dermatitis(AD). Methods: 35 patients, 2 to 14 years of age, with mild to moderate AD were treated with Phellinus linteus grown on germinated brown rice powder per oral, $6{\sim}12g$/day adjusted by the body weight, divided three for up to 12 weeks without other treatment. The mean age of patients was 7.3 years. The disease activity has been monitored by objective SCORAD index. The symptom score of the pruritus was measured by using a traditional visual analogue scale (VAS). In addition, the global assessments of clinical response were also monitored by patients or their parents. Results: After 12 weeks of the study, significant reductions in mean severity scores and mean symptom scores were observed. The mean objective SCORAD indices of 35 patients decreased from 25.31${\pm}$8.96 to 18.73${\pm}$13.89(p<0.05). And the mean symptom score of pruritus assessment decreased from 7.37${\pm}$1.33 to 4.6${\pm}$2.3(p<0.05). The patient's global assessment of clinical response were also improved through the period of treatment(p<0.05). Conclusions: Phellinus linteusgrown on germinated brown rice may play a role in the treatment of mild and moderate AD in Children.