• Journal of Microbiology
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• 제39권2호
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• pp.121-125
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• 2001

Methanol extract prepared from the fruitbody of Phellinus linteus (EPL) showed anti-tumor and immune-stimulating activities. The invasion activity of Bl6-F10 melanoma cells through a reconstituted basement membrane to the collagen-coated lower surface of the filters was inhibited about 67% by EPL (100 $\mu\textrm{g}$/ml). Also, EPL inhibited the expression of the mRNA for MMP-2 and MMP-9. In vivo treatment of C57BL/6 mice (150 mg/kg) with EPL for 14 days, the pulmonary colonization was found to be inhibited about 75%. Using reverse transcriptionpolymerase chain reaction (RT-PCR) analysis, we found that cytokine IL-12 and INF-${\gamma}$ genes were induced by EPL. Furthermore, EPL stimulated the proliferation of CD4$\^$+/(33.5%) and CD8$\^$+/(17.7%) in mouse splenocytes.

• 한국생명과학회:학술대회논문집
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• 한국생명과학회 2007년도 제48회 학술심포지움 및 추계국제학술대회
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• pp.65.1-65.1
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• 2007

See Full Text

• 한국생명과학회:학술대회논문집
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• 한국생명과학회 2002년도 제35회 학술심포지움 및 춘계학술대회
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• pp.106-106
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• 2002

• 대한약학회:학술대회논문집
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• 대한약학회 2000년도 춘계총회 및 학술대회
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• pp.123.2-124
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• 2000

• 대한약학회:학술대회논문집
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• 대한약학회 2000년도 춘계총회 및 학술대회
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• pp.189.2-189.2
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• 2000

• 대한약학회:학술대회논문집
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• 대한약학회 2001년도 Proceedings of International Convention of the Pharmaceutical Society of Korea
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• pp.275.1-275.1
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• 2001

• Journal of Microbiology and Biotechnology
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• 제15권5호
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• pp.1028-1038
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• 2005

To elucidate phylogenetic relationships of Phellinus and its related genera, nuclear internal transcribed spacer and mitochondrial small subunit ribosomal DNA sequences from 65 strains were determined and compared. The combined dataset of two sequences increased informative characters and led to the production of trees with higher levels of resolution. Phylogenetic analysis of the combined dataset revealed thirteen evolutionary lineages and several unresolved species that were together subdivided into two large clusters consisting of oligonucleate species and binucleate species. These results coincided with previous cytological, morphological, and molecular studies. It is newly recognized that the Phellinus linteus complex forms a sister clade to Inonotus, and that Fulvifomes is somehow related to Inocutis. The Phellinus linteus complex of dimitic perennial taxa made an independent clade from Inonotus and suggested that hyphal miticity and fruitbody permanence had enough phylogenetic significance to keep the complex within the traditional genus Phellinus. Taxa lacking setae were clustered into Fulvifomes, Phylloporia, Inocutis, and Fomitiporia, and the first three were closely related sister groups, but Fomitiporia was a genus distantly related to them. Several taxa with branched setae were shown among distantly related genera. Molecular evidence indicated that the ancestral nuclear type could be a binucleate feature, and that there might be parallel gains of branched setae and parallel losses of setae in the Hymenochaetales.

• 한국식품저장유통학회지
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• 제15권5호
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• pp.736-742
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• 2008

전통 된장의 숙성기간 중 기능적 특성의 변화를 조사하기 위하여 상황버섯 추출물을 이용하여 된장을 담금하여 일정기간 숙성한 후, 된장의 protease 활성, 항돌연변이 활성, isoflavone 등을 측정하였다. Protease의 활성은 상황버섯 추출물을 이용한 실험구가 3.15 unit/mL로 대조구보다 높은 활성을 나타내었으며 tyrosinase의 저해 활성과 ACE 저해 활성은 실험구가 각각 45.78%와 55.18%로 대조구보다 약 20% 이상 높은 활성을 나타내었다. 항돌연변이 활성은 실험구가 S. enterica serovar Typhimurium TA100을 사용한 경우 변이원 MNNG와 NPD에 대한 항돌연변이 활성은 각각 90.42%와 82.57%, S. enterica serovar Typhimurium TA98을 사용한 경우 변이원 NPD와 NQO에 대한 항돌연변이 활성은 각각 60.28%와 50.33%로 대조구보다 높은 활성을 나타내었다. 수소 공여능은 실험구의 활성이 86.65%로 대조구의 61.69%보다 높게 나타났다. 상황버섯 추출물을 이용하여 제조한 된장의 isoflavon 중 glucoside인 daidzin은 실험구가 35.49 mg/kg높게 나타났으며 genistin은 실험구와 대조구 모두에서 검출되지 않았다. Aglycone인 daidzein과 genistein은 각각 263.01 mg/kg와 262.60 mg/kg로 대조구 보다 높은 함량을 나타내었다.

• Journal of Microbiology and Biotechnology
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• 제6권3호
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• pp.213-218
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• 1996

Hot-water extract, Fr. 1, of Phellinus linteus mycelium was fractionated into Fr. 2, 3, 4, and 5 by the difference of solubility in ethanol. The polysaccharide fractions were studied for their immunostimulating activity on in vitro T-independent polyc1onal antibody response to trinitrophenyl-haptened SRBC (sheep red blood cell). The Fr. 4 with the highest immunostimulating activity was subjected to DEAE-cellulose ion exchange chromatography and gave five fractions, 4-I, II, III, IV, and V. The in vitro immunostimulating assay of the five fractions showed that 4-I and 4-III had a similar activity to that of LPS but the other fractions had low activity. By analyses of chemical composition and HPLC, all fractions obtained were found to be heteropolysaccharide-protein complex. The molecular weights ranged from 9, 000 to 15, 000. Sugar analyses showed that glucose, galactose, mannose, arabinose, and xylose were main component. Uronic acid and amino sugar were also detected in the fractions. It should be noted that the molecular weight (15, 000) of 4-III was very small and the structure of 4-III may be different from the known immunostimulating branched $\beta$-(1longrightarrow3)-glucan.

• 한국약용작물학회지
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• 제13권5호
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• pp.213-218
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• 2005

Phellinus linteus (PL), one of the immune-regulatory substances, is recognized to play the role in the metabolic process on inflammation and immunity. It has been traditionally used in the oriental medicine to treat inflammatory related disease. The purpose of this study was to evaluate the effects of water extracts of PL on the mesenteric lymph node lymphocytes immune function in the ICR male mice. Control mice received vehicle only. The PL treated mice were administered the respective extract by oral gavages for 4 weeks. IgE concentrations in serum and MLN lymphocytes were significantly lower in PL treated mice than in control mice. PL increased the proportion of $CD4^+\;and\;CD8^+$ T cells in MLN lymphocytes. PL significantly decreased Th2 cytokine concentrations and mRNA expression levels in cytokine secretions. Therefore, water extracts of PL modulate inflammatory parameters through regulation of immunoglobulin production resulting from decreased Th2 cytokine secretion and mRNA expression levels and reduce pro-inflammatory cytokine secretion and mRNA expression in MLN lymphocytes.