• 농업과학연구
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• 제21권1호
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• pp.37-44
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• 1994

특수가금류(오골계, 꿩, 청둥오리)의 산육성(産肉性)을 조사한 결과를 요약하면 다음과 같다. 1. 공시 가금류의 생체중에서 오골계(烏骨鷄)와 청둥오리는 암수가 각각 1,013.0~1,138.8g, 1,304.0~1,311.8g 이었으며, 꿩은 이들보다 적었다. 도체율은 꿩이 70.9~72.2%, 오골계(烏骨鷄) 66.9~68.2%, 청둥오리는 65.4~65.9%이었다. 2. 총가식내장량의 생체중에 대한 비율은 청둥오리가 6.3%로 가장 높았고, 오골계(烏骨鷄)가 4.7~4.8%로 가장 낮았다. 3. 각 부위별 분할량에서 꿩은 경부(6.7~8.3%)와 배부(16.1~18.0%)의 비율이 오골계(烏骨鷄)와 청둥오리의 비율보다 낮았으며, 청둥오리는 경부(11.1~13.3%), 익부(14.6~16.2%) 및 배부(23.6~25.2%)의 비율이 다른 가금류보다 높았다. 4. 흉부와 퇴경부에서 발골한 가식육량의 비율은 흉부에서의 수율이 퇴경부에서보다 공시 가금류 모두 높았으며, 청둥오리에서는 껍질의 비율(17.6~22.9%)이 높은 반면 행과 오골계(烏骨鷄)에서는 뼈의 비율(15.6~20.6%)이 높았다.

• 한국가금학회지
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• 제24권3호
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• pp.107-116
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• 1997

In order to achieve optimal reproductive performance, reliable morphological and physiological basic data on the reproductive organs are desirable. Adult male Korean ring-necked pheasant in inactive(mid of January) and active state (end of April) were used in this study. In addition, five active state pheasants were received a single dose of 60Co-ray 500 rads each to damage the testes. The objective of this study was to investigate the distribution pattern of protein gene product (PGP) 9.5 and ${\alpha}$-tubulin in the pheasant testes of the active, inactive and ${\gamma}$-ray irradiated active states. The results obtained were summarized as follows 1. The seminiferous tubules collected in inactive states( mid of Jan) showed narrow lumen, and the spermatogonia and the Sertoli cell were well preserved. The PGP 9.5 immunoreactivity of these tubules showed a positive reaction in paranucleus area of the spermatogonia, and a positive reaction in a small number of the Leydig cells in the interstitium of the seminiferous tubules. 2. The seminiferous tubules were dilated in active state(end of April) as compared with the inactive state. The PGP 9.5 reactivity in these tubules showed a positive reaction in many Leydig cells in the interstitium of the seminiferous tubules, and the testes of ${\gamma}$-ray irradiated group showed partially weak reaction in the interstitium of the seminiferous tubules. 3. The ${\alpha}$-tubulin reactivity in the seminiferous tubules of the inactive testes was strongly positive in the cytoplasmic process of the Sertoli cell from the basal stem region to the apical ex-tension. From the broad part of the stem region to the luminal space, the active testes showed a strong positive reaction. The ${\gamma}$-ray irradiated groups showed diminished reaction in the basal region.

• 대한수의학회지
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• 제34권1호
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• pp.9-18
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• 1994

The morphological changes of Sertoli cells of the Korean native pheasant were studied in the active and inactive spermatogenic phases. Twenty-four male of the pheasants were studied in the active (April~June) and inactive(August~March) phase. These data are useful in studying the male genital organs of the Korean native pheasant. Light microscopic morphological changes of the Sertoli cells were studied on paraffin-embedded sections stained with hematoxylin-eosin stain. Ultrastructural changes of Sertoli cells were investigated of ultrathin section using electron microscope. Results are summarized as follows: During the active phase, the average diameter of seminiferous tubule was $245.33{\pm}29.93{\mu}m$ and was largely decreased by $94.50{\pm}14.10{\mu}m$, and the thickness of interstitial tissue was comparatively increased during the inactive phase. During the active phase, in the cytoplasmic process of Sertoli cell and lipid droplets appeared disperse. Well-developed smooth Endoplasmic Reticulum and microtuble were observed in the cytoplasmic process. The nuclei of Sertoli cells were adjacent to the basement membrane. The size of nuclei was reduced and nuclei of Sertoli cells were densely packed within the tubule. Few collagen fibers, fibroblast and various sizes of lipid droplets were observed in the interstitial cell of the seminiferous tubule.

• 한국수정란이식학회지
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• 제29권4호
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• pp.351-359
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• 2014

Phasianus colchicus is not only a beautiful bird but also a great value in science and under the threat of endanger. Hence, the aim of this study was to isolate the pheasant male germ cells (mGCs) and then induce them into elongated sperm-like cells in vitro. The mGCs were purified and enriched by a two-step plating method based on the different adherence velocities of mGCs and somatic cells. The percentage of the c-kit positive cells and c-kit negative cells examined by flow cytometry analysis (FCA) was 92.87% and 2.57%, respectively. Subsequently, the mGCs were induced for 48h in DMEM/F12 medium supplemented factors such as retinol acid, testosterone and bovine FSH, followed by 5 weeks in culture. We found that some elongated sperm-like cells appeared initially in vitro under inducement of stimulated factors. The elongated sperm-like cells showed in the expression of changed morphology and post-transcriptional marker such as spermatid associated (SPERT), spermatid perinuclear RNA binding protein (STRBP), round spermatid basic protein 1 (RSBN1) and SPER1L. Moreover, in DNA content identified assay, induced cells showed that the 1C DNA population markedly increased in differentiated group but it was not change in undifferentiated group. Successful in vitro differentiation of pheasant testicular germline cells into spermatids appears to offer extremely attractive potential for the conservation of endangered birds and treatment of male infertility.

• 한국가금학회지
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• 제28권3호
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• pp.267-274
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• 2001

부화후 성장하는 꿩 송과샘의 미세구조적 발달을 구명하고자 1일령, 1, 2 및 6개월령을 희생시켜 광학 및 전자현미경을 이용하여 다음과 같은 결과를 얻었다. 광학현미경적 관찰에서 꿩 송과샘의 실질은 피막에 의해 싸여 있고 여기에서 기시하는 결합조직에 의해 불완전한 소엽 (lobule)으로 구분되었다. 송과샘실질은 소엽상과 불완전한 여포살이었으며, 둥근 핵과 염색성이 옅은 송과샘세포와 약간 짙고 길쭉한 핵을 갖는 지주세포로 구성되어 있었다. 전자현미경적 관찰에서 송과샘실질에는 길쭉한 송과샘세포와 지주세포가 소엽의 중심부를 향해 배열되어 있었고 광학현미경에서 소엽의 중심부에는 매우 불규칙한 막성층판복합체이나 포상구조물이 출현하였다. 실질은 비교적 밝은 솔과 샘세포와 짙은 핵을 갖는 지주세포로 구성되어 있었다. 송과샘세포는 잔유성 광수용세포의 형태를 취하였는데, 그 첨단부위의 밝고 팽대된 세포질에는 세포소기관이 빈약하나 섬모, 약간의 유리리보솜과 사립체가 출현하였다. 첨단부위세포질과 핵위부위세포질 사이의 좁아진 경부는 지주세포와 연접복합체를 이루고 목부위세포질내에는 미세소관이 풍부하였다. 핵주위세포질은 풍부하고 다수의 사립체, 잘 발달된 골지장치, 풍부한 과립형질내세망 및 유리 리보솜 등을 함유하고 있었다. 또한 핵아래부위세포질에서 여포의 기저부위로 기저돌기를 내고 있었다. 송과샘세포의 핵아래부위 세포질 및 기저돌기에서 60∼90nm 크기의 치밀소포가 관찰된 것이 특이하였고 짙은 핵을 갖고 긴 독기를 갖는 것이 특징이었다. 이상의 결과는 꿩 송과샘세포가 광수용능은 것의 없고 분비기능을 주로 수행하는 소견이었으며, 성숙 꿩의 송과샘의 미세구조적 연구를 확인하여 번식기와 비번식기에 따른 비교연구가 수행되어야 할 것으로 사료된다.

• 사상체질의학회지
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• 제15권1호
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• pp.50-59
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• 2003

Purpose Five animals sounds which are come under five sounds(Gong, Sang, jiao, zhi, yu) which are compared with the musical scale. It is looking for similarity between five animals' sounds and the musical scale. Methods 녹음 record 1 ig machine 1. Five animals (cattle, horse, pheasant, pig, sheep) sounds has been recording on tape. 2. That was transfer to CSL(computerized speech lab) 3. That was analysed to pitch, formant 1,2,3. energy pitch 4. That analysed result (Pitch, formant 1,2,3. energy ratio) of five animals are calculated and compared with the five musical scale(five sounds) Result The ratio of five animals sounds is not consistent with the musical scale in any five item (pitch, formant 1,2,3. energy). Conclusion 1.The five musical scale has no similarity with the five animals sounds 2.The five sound is supposed to oriented form theoretical back ground of five-going not have no relative with the five animals sounds

• 대한수의학회지
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• 제42권3호
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• pp.299-308
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• 2002

The purpose of this morphometric study was to obtain detailed quantitative information on all cell types in the testis interstitium of Korean ring-necked pheasants combined with data on changes in the steroidogenic function of the testis during the breeding and nonbreeding seasons. Animals collected during the breeding season, testis weights, sperm production, serum testosterone levels, leuteinizing hormone-stimulated testosterone secretion, and the length of the seminiferous tubules were significantly (p < 0.05) increased as compared to the nonbreeding season. Seminiferous tubules occupied 93.25% of testis volume in the breeding season. Leydig cells constituted 0.82% of the testicular volume. The mean volume of an Leydig cell was $1039{\mu}m^3$, and each testis contained about 24.53 million Leydig cells. Testes of the pheasants during the nonbreeding season displayed a 98% reduction in testis volume that was associated with a decrease in the absolute volume of seminiferous tubules (98% reduction), tubular lumen(100%), interstitium(90%), blood vessels(84%), lymphatic spaces(97%), Leydig cells(79%), mesenchymal cells(51%), and myoid cells(61%). The number of Leydig cells, mesenchymal cells, myoid cells per testis in the breeding season was higher (p < 0.05) than in the nonbreeding season. Although the average volume of a Leydig cell was 74% lower in the nonbreeding season, the average volume of a myoid and mesenchymal cell remained unchanged. These results demonstrate that there are a striking differences in the testicular structure of the Korean ring-necked pheasant in the breeding and nonbreeding seasons. Every structural parameter of the Leydig cell was pasitively correlated with both serum and LH-stimulated secretion concentrations of testosterone. Correlation of changes in hormonal status with morphometric alterations of all Leydig cell suggests that the Korean-ring necked pheasant may be used as a model to study structure-function relations in the avian testis.

• 대한수의학회지
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• 제34권4호
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• pp.665-678
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• 1994

This study was undertaken to investigate histological changes according to the radiosensitivity in the spermatogenic cells in Korean native pheasants. During spermatogenetic period, testes wete collected from male adult Korean native pheasant and they were used as experimental and control birds. The experimental group was divided into a single-dose whole body irradiation group(400, 600, 800 and 1000 rads) and a split-dose whole body irradiation groups(400/2, 600/2, 800/2 and 1000/2 rads). A Henseky's $^{60}Co$ ${\gamma}$-radiotherapy machine was used for this experiment and the dose rate of $^{60}Co$ ${\gamma}$-ray was 104 rads/min. The experimental birds were sacrificed at 24 and 72 hrs after irradiation and the control pheasants were sacrificed at the same time. General histological changes of seminiferous epithelial cells were observed by hematoxylin-eosin stain with light microscope. The results obtained are summarized as follows ; 1. In the single-dose and the split-dose irradiation groups, the average diameter of the seminiferous tubule was decreased compared with control group. 2. Seminiferous epithelial cells were more severely damaged after 72 hrs than after 24 hrs of single-dose irradiation of 400, 600 and 800 rads but the difference of cell injury was almost not observable with the elapsed time in the group of the single-dose irradiation of 1000 rads. 3. The damage of spermatogenic cells were more severe after 24 hrs than after 72 hrs of the split-dose irradiation of 400 rads but the split-dose irradiation of 600, 800 and 1000 rads were more severe after 72 hrs than after 24 hrs.

• 한국가축번식학회지
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• 제23권1호
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• pp.37-43
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• 1999

계절번식 조류인 꿩을 대상으로 번식기와 비번식기에 따른 세정관과 각 정자형성세포들의 직경, 길이와 면적 등의 변화상을 영상분석기를 이용하여 계측하였다. 그 결과는 다음과 같다. 1. 비번식기 곡세정관은 번식기보다도 약 2.56 배(38.94%) 위축되었다. 비번식기 세정관내에는 세르툴리세포와 정조세포만이 출현하였다. 2. 번식기 정조세포 핵과 세포질의 면적비는 28.71%이었으며, 비번식기의 면적비는 29.11%로 유의성은 없었다. 하지만 비번식기의 정조세포핵과 세포질 면적, 직경 및 둘레는 번식기보다는 증가했다. 3. 번식기 정모세포의 발달과정 중에서 태사기 상태에서 세포면적에 대한 핵의 면적비는 37.40%로 각 정자형성세포들 중에서 가장 높았다. 4. 원형에 가까운 정자세포의 세포면적에 대한 핵 면적비는 22.53%이었다.

• 한국가금학회지
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• 제22권1호
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• pp.1-6
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• 1995

A total of 514 birds were used to investigate the influence of body weight and shank length at hatch on the body weights at various ages in growing pheasant. Statistical model included the terms of hatch and sex as fixed effects and the two covariates of body weight and shank length at hatch. In this model, the effects of hatch and sex on the body weights at the age of 4, 8, 12, 16 and 20 wk, and the average daily gains from hatch to 8 wk and from 8 to 16 wk of age were highly significant(P<0.01). All the regression coefficients of body weights and average daily gains on the body weight at hatch were also significant(P<0.01). Their estimates were 3.05.7.21. 13.89, 15.18 and 15.33 for the body weights at 4. 8, 12, 16 and 20 wk of age ; 0.111 and 0.142 for the average daily gains from hatch to 8 wk, and from 8 to 16 wk of age, respectively. On the shank length, only the regression coefficients of the body weights at 4 and 8 wk of age and the average daily gains from hatch to 8 wk of age were significant(P<0.01). Results of this study suggest that body weight at hatch do significantly affect the body weights in the growing periods up to' the 20 wk of age, but the shank length at hatch influences the body weights only at early age.1)