• Journal of Plant Biology
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• 제26권4호
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• pp.191-196
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• 1983

The isolatin and culture of protoplasts from hypocotyl originated callus of Phaseolus vulgaris cv. Damyang were carried out. The maximum protoplast yield of 4.6$\times$105 per gram fresh callus, using the 13-day-old callus, was obtained by digeston for 6 hours in the enzyme solution. After 10 day-culture of the isolated callus protoplsts, plating efficiency was 50%. Thereafter, cell cluster medium, and followed by leading to callus formation on an agar medium after 3 weeks of the liquid culture.

• Journal of Plant Biology
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• 제27권3호
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• pp.173-178
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• 1984

Callus culture of Phaseolus vulgaris L. was carried out to examine the ability to grow on cytokinin-free medium. Of the sixteen cultivars of P. vulgaris, eight were classified as completely cytokinin-autonomous phenotype and five were found to be cytokinin-dependent phenotype. Intermediate phenotype was shown in three cultivars. Using cv. Palgong and ca 21 as cytokinin-dependent genotypes, the genotype responses to the cytokinin requirements of callus tissue were studied in detail. The callus tissue of cv. Palgong and ca 21 were never habituated in cytokinin-free medium, regardless tissue origin and cytokinin concentration in previous passages. The result suggests that cytokinin dependency of callus tissue of P. vulgaris cv. Palgong and ca 21 may be due to inactivation of cytokinin biosynthetic pathway.

• Bulletin of the Korean Chemical Society
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• 제25권7호
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• pp.955-956
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• 2004

• Bulletin of the Korean Chemical Society
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• 제30권10호
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• pp.2422-2424
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• 2009

• Bulletin of the Korean Chemical Society
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• 제28권2호
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• pp.175-176
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• 2007

• 한국식품과학회지
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• 제33권1호
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• pp.113-116
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• 2001

고지방과 고콜레스테롤 식이에 검정콩 추출물을 투여하지 않는 군과 검정콩 추출물 농도를 달리하여 투여한 흰쥐의 혈청 지질조성에 미치는 영향을 조사하기 위해 체중 $100{\pm}10\;g$의 S-D(Sprague-Dawley)계 수컷 흰쥐 49마리를 콜레스테롤을 투여하지 않는 표준지방식이군, 각각 콜레스테롤을 투여한 표준지방식이군, 고지방식이군 및 고지방식이군에 검정콩 추출물 농도를 달리하여 투여한 군으로 분류하여 4주 동안 사육하였다. 체중 증가량은 검정콩 추출물을 투여하지 않는 군에 비하여 검정콩 추출물 투여군이 약간 감소하였으나 유의적인 차이는 없었으며 식이섭취량은 각 군에 따른 유의적인 차이는 나타나지 않았다. 혈청 중 중성지질과 총 콜레스테롤 농도는 검정콩 추출물을 투여하지 않는 군에 비하여 검정콩 추출물 투여군에서 낮게 나타났다. HDL-, LDL-cholesterol에 대하여는 고지방식이와 검정콩 추출물의 투여후 혈중 지질 개선 효과가 어느 정도 있는 것으로 나타났다.

• Journal of Forest and Environmental Science
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• 제24권2호
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• pp.61-68
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• 2008

Acrylamide is present as a contaminant in heated food products, predominantly from the precursor asparagine. Nonanchored inter simple sequence repeats (ISSRs) are arbitrary multiloci markers produced by PCR amplification with a microsatellite primer. In order to assess the feasibility of microsatellite primers as markers for DNA damage, the study was conducted on common bean (Phaseolus vulgaris L.) exposed to different concentrations of acrylamide. Polymorphisms were abundant among plant samples treated with acrylamide in comparison to control (untreated one) tested with 4- tri-nucleotide, 2 tetra-nucleotide, and 3- dinucelotide primers. The primer (CCG)4 was the best tested primer to generate polymorphism between the DNA of plants treated or not by acrylamide. Polymorphisms became evident as the presence and absence of DNA fragments in treated samples compared with the untreated one. The highest number of DNA variation on ISSR patterns was observed at the micromollar concentrations of acrylamide. Acrylamide was able to induce DNA damage in non concentration-dependent manner with effectiveness at micromollar concentrations. This study demonstrated that ISSR markers can be highly reliable for identification of DNA damage induced by acrylamide.

• Journal of Plant Biology
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• 제32권4호
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• pp.323-330
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• 1989

This work was carried out to elucidate effects of endogenous and exogenous IAA transport on adventitious root formation in Phaseolus vulgaris hypocotyl cuttings. For inverted or normal incubation in distilled water, the adventitious root is always formed at the morphological base but not at the morphological apex. For inverted incubation, in both distilled water and certain chemical solution, the root formation is retarded more at the first stage (0-24 hr) than at the second stage (24-48 hr). When p-chlorophenoxyisobutyric acid (PCIB) was applied to the cuttings at the first stage, theroot formation was inhibited more than at the second stage. Treatment of 2,3,5-triiodobenzoic aicd (TIBA)markedly inhibited the adventitious root formation in Phaseolus vulgaris hypocotyl cuttings. This inhibition influenced the root according to the applied stage and period. Therefore, the root formation is more related to the stage of root primordium formation than to the stage of root elongation from the primordium. Inhibition of auxin transport oraction by TIBA or PCIB could also be reversed when hypocotyl cuttings are incubated in exogenously applied IAA solution.

• Food Science and Biotechnology
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• 제14권5호
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• pp.672-675
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• 2005

Two anthocyanins were isolated from 1% HCl-20% methanol extracts of KG 97287 black common bean (Phaseolus vulgaris L.) using semipreparative, high-performance liquid chromatography (HPLC). The anthocyanins were identified using a combination of LC/ES-mass spectrometry (MS) and spectroscopic methods of UV-Vis, $^1H-$ and $^{13}C-$ nuclear magnetic resonance (NMR). The chemical structures of these two anthocyanins were elucidated as delphinidin 3-glucoside and petunidin 3-glucoside and their contents in KG 97287 black common bean seed coats were determined to be $2.614{\pm}0.11$ and $0.167{\pm}0.01\;mg/g$, respectively. These contents were lower than reported internationally and we recommend the introduction into Korea of high anthocyanin varieties of black common bean.

• Journal of Plant Biology
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• 제16권1_2호
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• pp.12-16
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• 1973

The inhibitory effect of Inula sesquiterpene lactones on the growth of Phaseolus vulgaris was tested and the abnormality of the stem organization caused by the lactones was also examined. The longitudinal growth of the young stem and the expansion of the young leaf were stopped by the application of the lactones. However, this inhibitory effect was appeared and strictly restricted within the treated area. So the young shoot was observed for possible bending as a result of the unilateral application of the lactones. When the application of the lactones into the medium, the growth of the plant was entirely repressed. However, the growth of shoot and re-initiation of root were started after the plant was transfered to the lactone free medium. And partial reversal of inhibition of the stem growth was achieved by the additions of gibberelline and the lactones.