• 한국전자파학회논문지
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• 제26권3호
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• pp.292-299
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• 2015

본 논문에서는 3-레벨 인코딩 기법을 적용하여 시스템의 효율과 선형성을 개선할 수 있는 새로운 구조의 EER 송신기를 제안하였다. 제안된 송신기는 첨두 전력 대 평균 전력비에 상관없이 동일한 크기의 신호만을 증폭하고, 채널대역 내의 양자화 노이즈를 감소시켜 높은 효율을 얻을 수 있으며, 포락선 신호와 위상 신호 간 시간 부정합 특성을 개선하여 높은 선형성을 가질 수 있도록 하였다. 130 nm CMOS 공정으로 제작된 송신기 칩은 8.5 dB의 첨두 전력 대 평균전력비를 갖는 LTE 20 MHz 신호에 대해 2.13 GHz의 반송주파수에서 3.7 %의 오류 벡터 크기와 37.5 dBc의 인접 채널 누설비 특성을 보인다.

• BMB Reports
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• 제33권2호
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• pp.184-187
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• 2000

Prostaglandin $E_2$ ($PGE_2$) plays an important role in the regulation of various gastric functions, and the growth-inhibitory activities on tumor cells are studied in vitro and in vivo. Although the mechanisms have attracted many researchers in the past decade, the molecular mechanisms of cell cycle arrest, or induction of apoptosis by $PGE_2$, is unclear. We investigated the effects of $PGE_2$ on the growth of the human gastric carcinoma cell line SNU1 and genes that are regulated by $PGE_2$ and isolated them using differential display RT-PCR (DD RT-PCR). FACS analysis suggested that SNU1 cells were arrested at the G1 phase by $PGE_2$ treatment. This growth inhibitory effect was in a time- and dose-dependent manner. Treatment of SNU1 cells with $10\;{\mu}g/ml$ $PGE_2$, followed by DD RT-PCR analysis, revealed differently expressed bands patterns from the control. Among the differently expressed clones, we found an unidentified cDNA clone (HGP-27) overexpressed in $PGE_2$-treated cells. The full-length cDNA of HGP-27 was isolated using RACE, which consisted of a 30-nt 5'-noncoding region, a 891-nt ORF encoding the 296 amino acid protein, and a 738-nt 3'-noncoding region including a poly(a) signal. This gene was localized on the short arm of chromosome number 11. Using the Motif Finder program, a myb-DNA binding repeat signature was detected on the ORF region. The COOH-terminal half was shown to have similarity with the $NH_3$-terminal domain of thioredoxin (Trx). This relation between HGP-27 and Trx implied a potential role for HGP-27 in modulating the DNA binding function of a transcription factor, myb.

• Journal of Microbiology and Biotechnology
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• 제17권6호
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• pp.952-959
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• 2007

His-His-Leu (HHL), a tripeptide derived from a Korean soybean paste, is an angiotensin-I-converting enzyme (ACE) inhibitor. We report here a method of producing this tripeptide efficiently by expressing tandem multimers of the codons encoding the peptide in E. coli and purifying the HHL after hydrolysis of the peptide multimers. The HHL gene, tandemly multimerized to a 40-mer, was ligated with ubiquitin as a fusion gene (UH40). UH40 was inserted into vector pET29b; the UH40 fusion protein was then produced in E. coli BL21. The recombinant UH40 protein was purified by cation-exchange chromatography with a yield of 17.3mg/l and analyzed by matrixassisted laser desorption ionization (MALDI) time-of-flight (TOF) mass spectrometry and protein N-terminal sequencing. Leucine aminopeptidase was used to cleave a 405-Da HHL monomer from the UH40 fusion protein and the peptide was purified using reverse-phase high-performance liquid chromatography (HPLC) on a C18 HPLC column, with a final yield of 6.2mg/l. The resulting peptide was confirmed to be HHL with the aid of MALDI-TOF mass spectrometry, glutamine-TOF mass spectrometry, N-terminal sequencing, and measurement of ACE inhibiting activity. These results suggest that our production method is useful for obtaining a large quantity of recombinant HHL for functional antihypertensive peptide studies.

• The Korean Journal of Physiology and Pharmacology
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• 제13권3호
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• pp.241-249
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• 2009

Although extracellular $Ca^{2+}$ entry through the voltage-dependent $Ca^{2+}$ channels plays an important role in the spontaneous phasic contractions of the pregnant rat myometrium, the role of the T-type $Ca^{2+}$ channels has yet to be fully identified. The aim of this study was to investigate the role of the T-type $Ca^{2+}$ channel in the spontaneous phasic contractions of the rat myometrium. Spontaneous phasic contractions and $[Ca^{2+}]_i$ were measured simultaneously in the longitudinal strips of female Sprague-Dawley rats late in their pregnancy (on day 18 ${\sim}$ 20 of gestation: term=22 days). The expression of T-type $Ca^{2+}$ channel mRNAs or protein levels was measured. Cumulative addition of low concentrations (< 1 ${\mu}M$) of nifedipine, a L-type $Ca^{2+}$ channel blocker, produced a decrease in the amplitude of the spontaneous $Ca^{2+}$ transients and contractions with no significant change in frequency. The mRNAs and proteins encoding two subunits (${\alpha}$ 1G, ${\alpha}$ 1H) of the T-type $Ca^{2+}$ channels were expressed in longitudinal muscle layer of rat myometrium. Cumulative addition of mibefradil, NNC 55-0396 or nickel induced a concentration-dependent inhibition of the amplitude and frequency of the spontaneous $Ca^{2+}$ transients and contractions. Mibefradil, NNC 55-0396 or nickel also attenuated the slope of rising phase of spontaneous $Ca^{2+}$ transients consistent with the reduction of the frequency. It is concluded that T-type $Ca^{2+}$ channels are expressed in the pregnant rat myometrium and may play a key role for the regulation of the frequency of spontaneous phasic contractions.

• BMB Reports
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• 제32권1호
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• pp.86-91
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• 1999

The sprT gene encoding Streptomyces griseus trypsin (SGT) was introduced into Streptomyces lividans TK24 and Streptomyces lividans 1326 to study which strain would be better to overexpress the extracellular proteinase. Various media with different compositions were also used to maximize the productivity of SGT in heterologous hosts. The SGT productivity was best when the transformants of S. lividans TK24 and 1326 were cultivated in R2YE medium, and their relative trypsin activity of the culture broth measured with an artificial chromogenic substrate, N-${\alpha}$-benzoyl-DL-arginine-${\rho}$-nitroanilide, were 382 units/ml and 221 units/ml, respectively. They produced high levels of SGT in GYE medium but relatively lower than those in R2YE medium, and negligible amount of SGT was produced in Ferm, RASF, LIVID, and NDSK media. Considering non-SGT associated activity in Pronase powder, it was estimated that the transformant of S. lividans TK24 can produce SGT in R2YE 3.5 times more than the amount by S. griseus 10137 from which the sprT gene had been originated. The growth of S. lividans reached the maximum level of cell mass at 5 d of culture, but SGT production started in the stationary phase of cell growth and kept increasing until the ninth day of culture in R2YE medium, but in GYE media the productivity reached at the maximum level at 7 d of cultivation.

• Investigative Magnetic Resonance Imaging
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• 제24권3호
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• pp.95-122
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• 2020

In this article, we evaluated the performance of radiofrequency (RF) coils in terms of the signal-to-noise ratio (S/N) and homogeneity of magnetic resonance images when used for ultrahigh-frequency (UHF) 7T magnetic resonance imaging (MRI). High-quality MRI can be obtained when these two basic requirements are met. However, because of the dielectric effect, 7T magnetic resonance imaging still produces essentially a non-uniform magnetic flux (|B₁|) density distribution. In general, heterogeneous and homogeneous RF coils may be designed using electromagnetic (EM) modeling. Heterogeneous coils, which are surface coils, are used in consideration of scalability in the |B₁| region with a high S/N as multichannel loop coils rather than selecting a single loop. Loop coils are considered state of the art for their simplicity yet effective |B₁|-field distribution and intensity. In addition, combining multiple loop coils allows phase arrays (PA). PA coils have gained great interest for use in receiving signals because of parallel imaging (PI) techniques, such as sensitivity encoding (SENSE) and generalized autocalibrating partial parallel acquisition (GRAPPA), which drastically reduce the acquisition time. With the introduction of a parallel transmit coil (pTx) system, a form of transceiver loop arrays has also been proposed. In this article, we discussed the applications and proposed designs of loop coils. RF homogeneous coils for volume imaging include Alderman-Grant resonators, birdcage coils, saddle coils, traveling wave coils, transmission line arrays, composite right-/left-handed arrays, and fusion coils. In this article, we also discussed the basic operation, design, and applications of these coils.

• Journal of Plant Biotechnology
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• 제30권4호
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• pp.329-334
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• 2003

D-type cyclin은 호르몬과 영양분의 영향을 받아 세포주기의 G1기에서 S기로 전환을 조절하는 인자이다. 우리는 감자에서 이 유전자를 분리 해냈고, 염기서열 분석을 통하여 D3 cyclin으로 분류하였다. 그리고 StCycD3;1이라 명명하였다. 다른 D cyclin유전자가 세포 분열이 활발한 조직에서 발현되는 것과 같이 StCycD3;1은 감자의 괴경, 뿌리, 꽃, 잎, 줄기, 뿌리줄기, 복지에서 다양한 발현 양상을 보였고, 영양분의 하나인 sucrose에 의하여 발현이 유도되는 것을 확인하였다.

• 방송공학회논문지
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• 제21권2호
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• pp.180-191
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• 2016

일반 가시광선 영상의 확대를 위한 알고리즘을 선명도가 떨어지는 적외선 영상에 적용시켰을 때 개선효과가 미흡한 문제점을 해결하기 위해 본 논문은 적외선 영상을 위한 영상 확대 알고리즘을 제안한다. 제안 알고리즘은 적외선 영상이 가시광선 영상에 비해 디테일이 적다는 특성과 에지 영역에 대해 사람이 시각적으로 민감하다는 특성을 고려하여 ADRC(Adaptive Dynamic Range Coding)와 같은 단순한 분류기법을 적용시켰으며, 에지 영역에 대해서만 알고리즘을 적용시켜 연산량을 절약한다. 또한 확대 영상의 선명도 개선을 위해 합성과정에서 전처리나 후처리를 추가시키는 방법 대신 학습과정에서 전처리를 추가하여 합성과정에서 연산량 증가 없이 확대 영상의 선명도를 개선하였다. 제안 알고리즘은 크게 학습과정과 합성과정으로 나뉜다. 이와 같은 방법으로 영상 확대 알고리즘을 수행하였을 때 최신의 영상확대 기법인 A+ (Anchored neighborhood regression)기법 대비 JNB(Just Noticeable Blur)수치가 평균 0.0201만큼 높은 결과를 확인할 수 있었다.

• 한국통신학회논문지
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• 제24권7A호
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• pp.1028-1035
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• 1999

burst-mode TDMA 시스템에 사용되는 모뎀에서는, 데이터 전송효율을 증가시키면서 동시에 만족할만한 BER 성능을 얻기 위해서, 반송파 포착(acquisition)성능 및 추적(tracking)성능이 우수하여야 한다. 본 논문에서는 이를 위한 새로운 결정지향 반송파 복원(decision-directed carrier recovery) 알고리즘을 제안하였다. 제안한 방식은 PLL(Phase Locked Loop)을 사용하지 않기 때문에 빠른 반송파 포착이 가능하며, 가우시안 잡음이 부과된 입력신호를 수신단 전단에서 억압하는 사전 필터링(pre-filtering) 방식을 적용함으로 반송파 추적성능이 우수하다. 시뮬레이션을 통하여 제안한 방식의 BER 성능 및 포착 성능을 고찰해본 결과, 수신부에서 비교적 정확한 국부 발진기(local oscillator)를 채용하는 경우에는 기존의 방식에 비해 특히 BER 성능이 우수하였다. 이는 기존의 방식과는 달리 제안한 방식에서는 low SNR 환경에서도 cycle slip이 거의 발생하지 않는 장점이 있기 때문이다.

• 한국통신학회논문지
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• 제40권4호
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• pp.619-627
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• 2015

본 논문은 비음수 행렬 인수분해(NMF)를 이용한 음성향상 기법을 다루고 있다. 음성과 잡음에서 적절한 훈련을 통해 각각의 기저(basis) 행렬을 구하고 이 행렬들을 이용하여 두 음원을 분리 하는 것이다. 그 중에서도 음성향상의 성능은 사용하게 되는 기저 행렬에 따라 크게 달라짐을 보인다. 기존의 독립적으로 구한 음성 기저 행렬에 비해서, 잡음 데이터를 복원하는데 부적합한 방향으로 최적화시킨 음성 기저 행렬을 사용하였을 때 더 높은 음성향상 성능을 보임을 실험으로 확인하였다. 이 때 잡음 데이터의 복원 오차 자체를 크게 해주는 방향과 해당 인코딩 행렬(encoding matrix) 원소의 값을 작게 해주는 두 가지 방법을 적용하여 비교하였다. 좀 더 음성 복원에만 특화된 기저 행렬을 구함으로서 음성 기저 행렬이 잡음 데이터 복원에 사용되는 것을 최소화 하였다. 실험 결과에서는 perceptual evaluation speech quality값과 signal to distortion ratio를 지표로 사용하였고, 기존 기법에서 사용하는 기저 행렬 보다 더 높은 성능을 보임을 확인 하였다.