• Journal of Bio-Environment Control
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• v.16 no.4
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• pp.372-378
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• 2007

This study was carried out to investigate the effect of potassium concentrations in fertigation solution on growth and development of nutrient deficiency symptoms of leaf perilla (Perilla frutesens). The nutrient concentrations in above ground plant tissue, petiole sap and soil solution of root media were also determined. Potassium deficiency symptoms developed in older leaves with marginal necrosis. The brown areas on the lower leaves enlarged rapidly and the margins became scorched. Elevation of K concentrations in the fertigation solution up to 8 mM increased the crop growth in leaf length, stem thickness, and fresh and dry matter production of above ground plant tissue. However, that decreased the chlorophyll contents. The 8.0 mM K treatment which showed the greatest growth had 5.01 g in dry weight and 2.76% in K content of above ground plant tissue, suggesting that maintaining K content higher than 1.7% is necessary for good growth of Perilla frutesens. The K concentrations in petiole sap and soil solution of 8.0 mM treatment were $12,289mg{\cdot}kg^{-1}\;and\;11.65mg{\cdot}L^{-1}$, respectively. These indicated that K fertilization to maintain higher than $8,700mg{\cdot}kg^{-1}$ in petiole sap and $4.5mg{\cdot}L^{-1}$ in soil solution are necessary to ensure good crop growth.

• Journal of Korean Society of Forest Science
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• v.95 no.3
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• pp.261-267
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• 2006

A nursery trial comprising 109 progenies of 5 populations of Cornus kousa was analysed using multivariate analysis of 12 quantitative traits (growth and leaf form). The aim of this study was to examine geographic variation of the species based on the single, existing nursery trial to support a genetic resource conservation plan of Cornus kousa in Korea. Nested Anova showed that there were statistically significant differences among populations as well as among families within populations in all 12 quantitative traits. In 10 of 12 traits, variance components among families within populations were higher than those among populations. Cluster analysis using complete linkage method showed three groups to Euclidean distance 0.8. Among principal components, primary 2 principal components appeared to be major variables because of the loading contribution of 91.9%. The first contribution component was maximum width, vein number, blade length/petiole length and upper 1/3 width lower 1/3 width; the second one was height, diameter at root collar, blade length, upper 1/3 width, petiole length and petiole length/vein number, respectively. But all characters showed no significant difference with the pattern of geographic distribution.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.39 no.5
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• pp.480-488
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• 1994

Low seedling growth rates of white clover (Trifolium repens L.) have been limited its good establishment to pastures. The experiment was done to determine the effect of removal of cotyledon and unifoliolate on the growth and morphological characters of contrasting white clover cultivars for 8 weeks after the treatment. Individual plants of cv. Regal (large leaf), Louisiana S-l (medium-large leaf), Grasslands Huia (medium-small leaf) and Aberystwyth S184 (small leaf) were grown in 10cm plastic pot containing a 2:1:1 soil:sand:peat moss mixture until the cotyledon or unifoliolate stage and then removed one (C1) or two cotyledons (C2) at cotyledon stage, and unifoliolate only (U), unifoliolate and one cotyledon (DC1) or unifoliolate and two cotyledons (DC2) at the unifoliolate stage. To measure the removal effect on biomass and morphological characters (leaf area, petiole and stolon lengths, growing tips and leaves), plants were sampled 4, 6 and 8 weeks after the treatment. Intact plants had greater biomass and morphological characters than removal-treated ones, Removal treatments at cotyledon stage, C1 and C2, were decreased more biomass and morphological characters than removal ones at unifoliolate stage while the severer cotyledon removal, the more reduction. Stolon length per plant and petiole length markedly inclined 6 weeks after the treatments although biomass and the other characters continuously did. Relatively large-leaved cultivar had more biomass, leaf area per plant and longer petiole than the other(s) but the reverse results were true in stolon length, growing tips and no. of leaves per plant. Biomass was linearly increased with increased leaf area but the earlier and severer removal, the less slope. The severer damage of cotyledon and unifoliolate had detrimental effects on the growth and aftermath establishment of white clover

• KOREAN JOURNAL OF CROP SCIENCE
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• v.39 no.3
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• pp.245-255
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• 1994

Weak persistence of white clover (Trifolium repens L.) under continuous grazing management has been limited its availability in the mixture with grasses. The experiment was carried out to determine the effect of defoliation interval on the regrowth and morphological characters of the clover cultivars. Individual plants of Regal (large leaf), Grasslands Huia (medium-small leaf) and Aberystwyth S184 (small leaf) were grown in 15cm plastic pot containing a 1: 2: 1 soil:sand:Promix mixture for 55 days, and then clipped to remove all fully expanded leaves every 7, 14 or 28 days. For the analysis of the cultivar response, plants were sampled on the final harvest date(0), and 1, 3, 7, 14 and 28 days after the final harvest date. Harvested dry weight of all cultivars declined as defoliation interval was increased, and that of Regal was the highest compared to the other cultivars. During the regrowing period, increase of total plant dry weight was due to that of leaf and petiole dry weight, and that of Osceola was greater than the others. Although total leaf area and mean single leaf area were increased during the regrowing period, they were reduced with increased defoliation interval and those of Osceola were the greatest until 14-days regrowth. S184 possessed the most number of leaves and Osceola did the longest petiole since 14-days regrowth although the more defoliation, the less nunber of leaves per plant and the shorter petiole. Stolon length and growing tips of all cultivars increased steeply during regrowth, while they were decreased with increased defoliation interval and those of S184 were the highest. More frequent defoliation had detrimental effects on regrowth of white clover, although larger leaf type was productive but less persistent in a mixture with grasses than smaller leaf type.

• Korean Journal of Plant Resources
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• v.19 no.1
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• pp.150-160
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• 2006

This study was performed to provide basic information of the development and breeding of new cultivars of Hibiscus syriacus L., which have more beautiful and diverse flowers. Morphological characteristics of the leaves and genetic variation of the leaves of two Jeoktanshim-line cultivars, Bulsae and Pyungsung, were crossed each other. The result of the cross between Bulsae and Pyungsung are as follows: Mean leaf length and width were 6.09cm and 3.36cm, respectively. Mean petiole length was 1.08cm, and mean shoulder width and incision depth were 1.50cm and 0.84cm, respectively. Mean leaf base angle was $98.56^{\circ}$. Leaf index and petiole length were the most important components of leaf characteristics. Two groups were clustered in distance level 5.4. The result of the cross between Pyungsung and Bulsae are as follows: Mean leaf length and width were 5.25cm and 3.10cm, respectively. Mean petiole length was 0.87cm, and mean shoulder width and incision depth were 1.50cm and 0.76cm, respectively. Mean leaf base angle was $107.87^{\circ}$, which was the widest among ten cross combinations. Two groups were clustered with seven leaf characteristics in distance level 4.2. Group I included two small groups, and group II included four small groups.

• Korean Journal of Plant Tissue Culture
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• v.26 no.2
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• pp.121-126
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• 1999

Calli were induced from the petiole explants of Pimpinella brachycarpa on MS medium supplemented with 0.5 mg/L 2,4-D and 0.1 mg/L BA after four weeks of culture. Compact clusters of small and dense cells among these calli were selected and suspension-cultured as the source of embryogenic calli. When transferred to MS medium with 0.1 mg/L NAA, the suspension-cultured cells grew to embryogenic callus. Somatic embryos derived from these embryogenic calli developed into plantlets. The cDNA library was constructed in the embryogenic callus and in order to screen the cDNA library, these cDNAs were plated at a density 1.5 $\times$ 10^5 plaques per 15 cm petridish. Among 19 clones showing preferential hybridization with petiole HD-Zip gene, five clones were obtained after second screening. Four clones among them, were highly homologous to P. brachycarpa shoot-tip Phz4 gene, but one clone, Phc5 was about 1.5 kb which has an extra 163 bp to 5' upstream of Phz4. The Phc5 was 1,531 bp containing poly A tails of 18 bases. ATG start codon for Phc5, was located at position 284 with an open reading frame of 906 by which encodes a polypeptide of 302 amino acids. The Phc5 protein revealed that the polypeptides between 135 and 195 contain a homeodomain as the `leucine zipper' motif.

• Journal of Korean Society of Forest Science
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• v.43 no.1
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• pp.1-5
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• 1979

The arrangement and the number of vascular bundle in petiole were investigated with the hybrid Populus alba${\times}$P. glandulosa and both parents. 1. The variation in number and the arrangement of the vascular bundle in petiole were smaller at middle point than at nearer point to the leaf blade. 2. A small variation was found in the arrangement of the vascular bundle within a tree, same clone and same species. 3. Five shapes of vascular bundle were recognized in the $F_1$ hybrid, 26.7 per cent of the $F_1$ hybrid has the same shape with P. alba, 13.3 percent with P. glandulosa and 53.3 per cent of the $F_1$ hybrid shows the $F_1$ shape caused by hybridization. 4. The hybrid clones which show the same shape with P. alba are 66-20-1, 66-6-8, 65-22-11 and 64-6-44, hybrid clones of 65-95, 66-14-93 have the same shape with P. glandulosa. Hybrid clones of 66-15-3, 67-6-3, 65-22-4, 66-26-55, 68-1-54, 66-14-99, 65-29-19, 66-25-5 have $F_1$ shape.

• Journal of Bio-Environment Control
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• v.25 no.3
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• pp.146-152
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• 2016

Due to complicate canopy structures of Irwin mangoes grown in greenhouses, it is difficult to determine their growth parameters accurately. Leaf area, leaf fresh weight, and leaf dry weight are widely used as indicators to diagnose the tree growth. Therefore, it is necessary to establish models that can non-destructively estimate these growth indicators. The objective of this study was to establish regression models to estimate leaf area, leaf fresh weight, and leaf dry weight of Irwin mangoes (Mangifera indica L. cv. Irwin) by using leaf length, leaf width, petiole length, and SPAD value. The input values of leaf length, leaf width, petiole length, and SPAD value of 6-year old Irwin mangoes were measured, and the corresponding output values of leaf area, leaf fresh weight, and leaf dry weight were also measured. After 14 models were selected among the existing models, coefficients of the models were estimated by regression analysis. Three models with higher $R^2$ and lower RMSE values selected. In validation the $R^2$ values for the selected models were 0.967, 0.743, and 0.567 in the leaf area, leaf fresh weight, and leaf dry weight models, respectively. It is concluded that this models will be helpful to conveniently diagnose the growth of the Irwin mango.

• Journal of Plant Biotechnology
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• v.29 no.1
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• pp.45-49
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• 2002

The leaf and petiole segments of Farfugium japonica were cultured to investigate the influence of growth regulators on their callus induction and plant regeneration. The callus induction and growth showed a good response both leaf and petiole on MS media supplemented with 1∼2 mg/L 2,4-D and 1∼2 mg/L BA. Callus induction and growth were more effective in petiole segments than leaf one. The highest percentage of plant regeneration was obtained from 60-day-old calli on MS medium supplemented with 1 mg/L NAA and 2 mg/L BA. When subcultured to the same medium for about 60 days, multiple shoots were developed from regenerating callus. The shoots produced roots after transferring to rooting medium containing 0.5 mg/L IAA. The plantlets over 50 mm in height were successfully acclimatized in vermiculite, and the survival rate was over 95%.

• Journal of Korean Society of Forest Science
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• v.73 no.1
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• pp.21-32
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• 1986

This study was done to classify 15 species in the genus Acer through tracking of the changing shape of the vascular branch from the base part to the terminal part of petiole by the anatomical method. The basic vascular branches were 3 ea, except A. mandshuricum 3-4 ea. The vascular bundle was separated, moved and connected from the base part to the terminal part of petiol, the number of separated vascular branch was 11-32 ea., the number of connected vascular branch was 0-17 ea., and the ratio of the separated vascular branch to the connected was 0.00-8.00. The maximum number of vascular branch in No, 39 of cross section part was observed in 12 species; A. ginnala and A. buergerianum were No. 11, A. truncatum and A. platanoides were No. 13, A. saccharium was No. 26. The number of the connected vascular branches with the main vascular branch were 0-6, while the number of the separated vascular branch were 0-7. On the ratio of separation to connection of the base vascular branch; "A", "B" and "C", the symmetrical shapes on the basis of "B" were A>BC, AB>C shape. The classical groups by the development of main vascular branch formation were obtained 7 groups of "a" through "g" shape. Especially, A. negundo and A. saccharinum were shown to have central vascular branches with unique changing patterns.