• Journal of Microbiology and Biotechnology
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• v.34 no.8
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• pp.1727-1737
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• 2024

The quality of tobacco is directly affected by macromolecular content, fermentation is an effective method to improve biochemical properties. In this study, we utilized CBHA (cellobiohydrolase A) glycosylase, which was expressed by Pichia pastoris, as an additive for fermentation. The contents of main chemical components of tobacco leaves after fermentation were determined, and the changes of microbial community structure and abundance in tobacco leaves during fermentation were analyzed. The relationship between chemical composition and changes in microbial composition was investigated, and the function of bacteria and fungi in fermentation was predicted to identify possible metabolic pathways. After 48 h of CBHA fermentation, the contents of starch, cellulose and total nitrogen in tobacco leaf decreased by 17.60%, 28.91% and 16.05%, respectively. The microbial community structure changed significantly, with Aspergillus abundance decreasing significantly, while Filobasidum, Cladosporium, Bullera, Komagataella, etc., increased in CBHA treated group. Soluble sugar was most affected by microbial community in tobacco leaves, which was negatively correlated with starch, cellulose and total nitrogen. During the fermentation process, the relative abundance of metabolism-related functional genes increased, and the expressions of cellulase and endopeptidase also increased. The results showed that the changes of bacterial community and dominant microbial community on tobacco leaves affected the content of chemical components in tobacco leaves, and adding CBHA for fermentation had a positive effect on improving the quality of tobacco leaves.

• Korean journal of applied entomology
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• v.60 no.1
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• pp.49-61
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• 2021

Integrated mite management provided a basic direction to early the fruit tree IPM. The early IPM concept was based on incorporation of the biological control for mite pests with the existing chemical control compatibly. Since then, the hypotheses and mechanisms of the interaction between species diversity and pest population dynamics have provided a broader understanding of mite-centered pest management in fruit tree ecosystems. Based on the principle of the ecosystem, biological control and pest management through habitat modification or manipulation are developing to the concept of agro-ecological engineering. In particular, the natural enemy diversity is dynamically changed according to the different cultivation environment in the management of mite pests, and the species composition of phytophagous mites is also changed by the environment for orchard management. This paper reviews the biological control of mites, which was the basis of apple IPM, and also re-examines the topics of species diversity and pest management, predacious mite diversity in relation to mite control and the change of species composition of mite pests in the sight of ecological engineering. Finally, we suggest a strategy for biological control of spider mites in apple orchards in Korea.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.27 no.4
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• pp.361-370
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• 1982

The effects of climatic factors on organisms lire variable and complex, and it, however, can be interpreted in terms of those on the distribution and those on the population densities. The distribution of an organism may largely be determined by the temperatures, except some temporal organisms which are depended on the air mass movements. Population density of an organism is determined by various climatic factors, such as previous winter temperature, temperature of growing season and rainfall. The start of growing season of the rice plants has been shifted to earlier since last decade in Korea. This may mean that the overall climatic condition during the growing season might be considerably different from those in past years, and such a difference in climatic conditions might have close relation with the recent status of the diseases and insect pests through direct effects on the physiology and population dynamics of the organisms, as well as through on the biotic associations of the pest organisms. The white back planthopper and brown planthopper have become the key insect pests in Korea in recent years. They are migratory and have high reproductive pontentials and more generations than average residential insects. The synchronization of the migrants and physiological condition of the rice plants seems to be the important factors in relation to the recent outbreaks of these insects; the high reproductive rate can be obtained with the growth stage of rice being 30-50 days after transplanting. The modication of the microclimate associated with high plant density and some other introduced new cultural techniques also have some relation with the outbreak. The key diseases of the rice are the blast disease, sheath blight and the bacterial leaf blight. For the rice blast, the seedling blast and leaf blast during the early growing season and the neck blast, have become more serious, the former may be related to hotbed nursery and the later may be related to the high humidity in early August, and synchronization of the heading time which has been shifted to early part from middle or late part of August. In general, for the rice diseases, the development of the new races have been the most serious which are largely resulted from the introduction of the new varieties, but it also seems to be related with the prolonged periods of the favorable condition associated with the shifted growing seasons. In general, the diseases and insect pest problems have become much more variable and complex, and control measures should be based on the thorough knowledge of the ecology of the pest organisms, that is, effects of various environmental factors on the disease cycle; spore release, spore deposition, infection, colonization and sporulation of the disease organisms, and those on the development, reproductive potentials, dispersal, age specific responses of the insects. The well organized real-time pest management systems, such as alfalfa weevil management system developed at the Purdue University in U.S., is the prime importance for the implementation of the pest management principles.

• Proceedings of the National Institute of Ecology of the Republic of Korea
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• v.5 no.1
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• pp.21-27
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• 2024

Harmful pet insects, if not controlled, can negatively affect people, plants and their surrounding environment. In Vietnam, all crops are regularly impacted by pest insects. In serious cases, crops can be totally destroyed by insect pests. Harmful insects that damage crops often grow fast and increase rapidly. Therefore, research on insects is crucial for managing pests, protecting crops, and forecasting pest situation in the following years. This study aimed to collect data regarding changes of pests on rice and corn as two main crops in four provinces in Red River Delta of Vietnam, including Thai Binh, Nam Dinh, Ha Nam, and Hung Yen, from 2018 to 2022. Primary data were collected from reports of government agencies and official statistics. Based on these data, this study evaluated changes of pest insects in five years, discussed reasons for such changes and response methods, and forecasted pest's behavior in the following years. Significant findings of this study include the fact that Vietnam has to face many difficulties to develop its agricultural sector. For insect management, an essential action is to do ground surveys to gather all related data including weather data, pesticide data, crop yield, and product quality. This information is meaningful for finding out causes of changes, understanding relationships between insects and surrounding factors, and predicting the situation in the following years.

• Mycobiology
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• v.43 no.1
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• pp.37-42
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• 2015

A mutant library of Cordyceps militaris was constructed by improved Agrobacterium tumefaciens-mediated transformation and screened for degradation features. Six mutants with altered characters in in vitro and in vivo fruiting body production, and cordycepin formation were found to contain a single copy T-DNA. T-DNA flanking sequences of these mutants were identified by thermal asymmetric interlaced-PCR approach. ATP-dependent helicase, cytochrome oxidase subunit I and ubiquitin-like activating enzyme were involved in in vitro fruiting body production, serine/threonine phosphatase involved in in vivo fruiting body production, while glucose-methanol-choline oxidoreductase and telomerase reverse transcriptase involved in cordycepin formation. These genes were analyzed by bioinformatics methods, and their molecular function and biology process were speculated by Gene Ontology (GO) analysis. The results provided useful information for the control of culture degeneration in commercial production of C. militaris.

• Parasites, Hosts and Diseases
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• v.56 no.4
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• pp.313-324
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• 2018

This article reviews Leptotrombidium deliense, including its discovery and nomenclature, morphological features and identification, life cycle, ecology, relationship with diseases, chromosomes and artificial cultivation. The first record of L. deliense was early in 1922 by Walch. Under the genus Leptotrombidium, there are many sibling species similar to L. deliense, which makes it difficult to differentiate L. deliense from another sibling chigger mites, for example, L. rubellum. The life cycle of the mite (L. deliense) includes 7 stages: egg, deutovum (or prelarva), larva, nymphochrysalis, nymph, imagochrysalis and adult. The mite has a wide geographical distribution with low host specificity, and it often appears in different regions and habitats and on many species of hosts. As a vector species of chigger mite, L. deliense is of great importance in transmitting scrub typhus (tsutsugamushi disease) in many parts of the world, especially in tropical regions of Southeast Asia. The seasonal fluctuation of the mite population varies in different geographical regions. The mite has been successfully cultured in the laboratory, facilitating research on its chromosomes, biochemistry and molecular biology.

• Parasites, Hosts and Diseases
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• v.52 no.1
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• pp.63-67
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• 2014

This paper describes a new species of chigger mite (Acari: Trombiculidae), Gahrliepia cangshanensis n. sp., from rodents in southwest China. The specimens were collected from Yunnan red-backed voles, Eothenomys miletus (Thomas, 1914), and a Chinese white-bellied rat, Niviventer confucianus (Milne-Edwards, 1871) in Yunnan Province. The new species is unique mainly in its number of dorsal setae (n=21), and it has the following features: fT (formula of palpotarsus)=4B (B=branched), fp (formula of palpal seta)=B/N/N/N/B (N=naked), a broad tongue-shaped scutum with an almost straight posterior margin, and 17 PPLs (posterior posterolateral seta) with a length of 36-43 ${\mu}m$. This chigger mite may also infect other rodent hosts and may be distributed in other localities.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.20 no.1
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• pp.440-446
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• 2019

Pine wilt disease occurred for the first time in Busan in 1988 and the damage has since been increasing. In 2005, a special law was enacted for pine wilt disease by Korea Forest Service. Incidences relating to the forest pest had been frequent and chemical control as well as physical control techniques had been applied to control it. Therefore, there is a need to reduce the damage caused by the pine wilt disease through intensive management such as continuous monitoring, control, and monitoring based on active control as well as management measures. In this study, the UAV-based monitoring method was proposed as an economical way of monitoring the forest pest. The efficiency of the existing method and UAV method had been analyzed, and as a result the study suggested that UAV can be used for forest pest monitoring and indeed improve efficiency. The UAV-based forest pest monitoring method has a cost reduction of about 50% compared with the conventional method and will also help to reduce the area where the survey was omitted.

• Journal of the Korean Society for information Management
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• v.38 no.2
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• pp.251-276
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• 2021

In 2021, the Korean National Police Agency is facing a critical time as it meets a period of drastic changes such as the introduction of autonomous police system, the reform of its criminal investigation system, and its great transformation to a truly responsible investigative agency in keeping with the inauguration of the National Office of Investigation. This study carries out PEST analysis, which is a macro environmental analysis technique, and examines the current situation of operating the libraries of metropolitan-city and provincial police agencies and affiliated institutions, in order to suggest the role and function of the Korean National Police Agency's libraries in the fourth industrial revolution era. Based on the findings, this study classifies the essential components of a library into facilities, books, and librarians, i.e. spatial composition, book composition, and human resources, in addition to library operational governance establishment and future-oriented library services; and proposes the future-oriented roles of the National Police Agency's libraries in the fourth industrial revolution era.

• International Journal of Industrial Entomology and Biomaterials
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• v.33 no.1
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• pp.15-23
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• 2016

One major constraint in the development and implementation of a successful and cost-effective area-wide integrated pest management (AW-IPM) programme with a SIT component for Bactrocera oleae (Diptera: Tephritidae) is the ability to produce a large number of high quality mass-reared individuals. The aim of this study was to develop a more efficient and practical egg collection system in an attempt to improve the mass-rearing of this species. The following basic parameters were examined: egg production per female, egg hatch, pupal recovery, pupal weight, adult emergence and percentage of fliers. Three different strains (Israel wild-type, France wild-type, and Greece laboratory) were tested and each strain was evaluated for six generations. Female flies of the Israel strain produced significantly more eggs per female than the other two strains, but egg hatch was significantly lower. Egg hatch of the France wild type and the Greece laboratory strain was similar. For all other parameters, there was no significant difference between strains; however, there was a significant generational effect for all parameters observed. As a result of this study, a protocol was developed for the mass-rearing of this species that included the use of large adult holding cages that could house up to 96,000 flies per cage. The newly developed method of egg collection using a flat wax panel as one of the sides of an adult holding cage proved to be cost-effective, efficient, making colony growth easier for industrial mass-rearing.