• Journal of Life Science
• /
• v.13 no.1
• /
• pp.99-104
• /
• 2003

Aromatic compounds are of major concern among environmental pollutants due to their toxicity and persistence. To monitor aromatic compounds in a real time with a better sensitivity, a new method of SPR (surface plasmon resonance) based on DNA chip (Biacore 3000) was developed here. It is thought that CapR regulatory protein as a complex with phenol, could bind to their corresponding promoter, Po. Biotinylated DNA oligomers for the promoter was synthesized by PCR and coupled onto streptoavidin-linked CM5-chip. CapR regulatory proteins were purified after cloning their genes in pET21a (+) vector and expressing proteins. The interaction was assessed by the system where the regulatory proteins flowed with or without phenol through the cells of DNA chip. CapR regulatory protein even in the presence of phenol had no response to its promoter, Po, suggesting that other factor(s) might be required for the activation of Po promoter. The present work reveals a promising possibility of the SPR-based DNA chip in monitoring specific environmental pollutants in a real time.

• The Journal of Korean society of community based occupational therapy
• /
• v.9 no.3
• /
• pp.31-39
• /
• 2019

Objective : The purpose of this study is to identify time management among college students and provide them with a basic resource for planning and educating them on efficient time management methods for improving the quality of life of all clients who receive occupational therapy services, including non-disabled people. Methods : This study is a survey of four-year university students using the questionnaire to know the relationship between structured time usage and quality of life. A total of 142 questions, excluding eight missing questionnaires, was compared and analyzed with using the general characteristics, time structure questionnaires (time structure questionnaires) and World Health Organization (WHOQOL-BREF). Results : The results of this study showed that there was a significant correlation between quality of life and structured time management, and between the sub-items of structured time management; sense of purpose, structured daily life, persistence, past direction and sleep intensity. Also, factors that affect the quality of life were sleep (β=.214), structured daily life (β=.203), a sense of purpose(β=.343), and past direction(β=.244) appeared in order. Conclusion : In the case of university student who is working within structured time, there were difficulties in showing satisfactory quality of life. These factors of poor quality of life were analyzed to affect structured daily life, sense of purpose, past direction, and sleep level.

• Journal of Applied Biological Chemistry
• /
• v.63 no.3
• /
• pp.243-248
• /
• 2020

The long-chained perfluoroalkyl acids (PFAAs), perfluorooctanoic acid (PFOA) and perfluorooctanesulfonic acid (PFOS), are a potential exposure risk in the environment, specifically for humans due to high levels of bioaccumulation, persistence, and toxicity. In the current study, the plant uptake factors (PUFs) of spinach and Welsh onion were investigated on the three different concentration levels of PFOA and PFOS in soil. Spinach and Welsh onion were divided into three residue groups, a control group and two levels of PFOA and PFOS. The PFAAs spiked soils were aged for six months and the extractable residue of PFOS in the aged soil was reduced to 30-59% of the initial spiked concentrations for PFOS, while PFOA showed almost the same initial spiked concentrations. The PUFs for PFOA and PFOS were 0.111-2.821 and 0.047-3.175 for spinach, and 0.203-0.738 and 0.035-0.181 for Welsh onion, respectively. The highest PUF values in both vegetable were displayed when the residual concentration of PFAAs were part-per-billion (ppb) or sub-ppb in soil.

• Asian-Australasian Journal of Animal Sciences
• /
• v.28 no.9
• /
• pp.1259-1265
• /
• 2015

The present study was performed to study the genetic relationship of productive life with production and type traits of Korean Holsteins at first three lactations. The data for the analysis from 56,054, 28,997, and 11,816 animals of first, second and third parity cows which were born from 2006 to 2011 were collected by Dairy Cattle Improvement Center, National Agricultural Co-operative Federation. Milk, protein and fat yields adjusted for 305 days and average somatic cell score considered as production traits and analyzed type traits were stature, strength, body depth, dairy form, rump angle, rump width, rear leg side view, foot angle, front attachment placement, rear attachment height, rear attachment width, udder cleft, udder depth, front teat placement and front teat length. A multi trait genetic analysis was performed using Wombat program with restricted maximum likelihood animal model composed of fixed effect of birth year, farm and the random effect of animal and random residual effect according to the traits. Heritability estimates of productive life were between 0.06 and 0.13. Genetic and phenotypic correlations between production and productive life traits ranged from 0.35 to 0.04 for milk, 0.16 to 0.05 for protein and 0.18 to 0.02 f 15-0034 (2nd) 150520 or fat. Somatic cells score showed a negative genetic and phenotypic correlation with productive life and also udder type traits, indicating that the selection for higher udder traits will likely to improve resistance to mastitis and persistence in the herd. Among all dairy form type traits, udder characters such as udder cleft showed a significant relationship with productive life. However, a specific change of heritabilities or correlations were not observed with the change of parity. Moreover, further studies are needed to further confirm the significance of the above traits and the effect of parity on above relationships in order to minimize both voluntary and involuntary culling rates while improving herd health and maintaining high yielding dairy cows.

• Journal of The Korean Society of Agricultural Engineers
• /
• v.61 no.1
• /
• pp.145-159
• /
• 2019

A good number of drought indices have been introduced and applied in different regions for monitoring drought conditions, but some of those are region-specific and have limitations for use under other climatic conditions because of the inherently complex characteristics of drought phenomenon. Standardized Precipitation Index (SPI) indices are widely used all over the world, including Bangladesh. Although newly developed, studies have demonstrated The Effective Drought Index (EDI) to perform better compared to SPIs in some areas. This research examined the performance of EDI to the SPI for detecting drought events throughout 35 years (1981 to 2015) in Bangladesh. Rainfall data from 27 meteorological stations across Bangladesh were used to calculate the EDI and SPI values. Results suggest that the EDI can detect historical records of actual events better than SPIs. Moreover, EDI is more efficient in assessing both short and long-term droughts than SPIs. Results also indicate that SPI3 and the EDI indices have a better capability of detecting drought events in Bangladesh compared to other SPIs; however, SPI1 produced erroneous estimates. Therefore, EDI is found to be more responsive to drought conditions and can capture the real essence of the drought situation in Bangladesh. Outcomes from this study bear policy implications on mitigation measures to minimize the loss of agricultural production in drought-prone areas. Information on severity level and persistence of drought conditions will be instrumental for resource managers to allocate scarce resources optimally.

• Korean journal of applied entomology
• /
• v.38 no.1
• /
• pp.47-52
• /
• 1999

Effects of organic matter content in soil on activity. vertical migration. and persistence of two nernaticides. carbofuran and ethoprophos. to root-knot nematode, Mrloitlog!~iei ~lcognita.w ere investigated. As the organic matter content increased. activity of the nernaticides tended to be reduced. Both nematicides exhibited control values of more than 80'k' to M. iix.o,yilit~iln 0-2 cm depth soil layer from the surface. regi~rdless of organic matter content in soil. In 2-4 cm depth soil layer. however. the control value of the neniaticides varied with the organic niatter content in soil. The control value of carbofuran in the soil layer was ranged from I0 to 30'2,. depending on the soil organic niatter content. In contrast. ethoprophos had no control value against M. i/ic.o,ytlitcr in the \oil layer, except that the nematicide had a control value of 30% when the organic matter content was 0.4%. Furthermore. ethoprophos had no effect on controlling M. i/ic.o,gtiitrr in soil layer of below 4cm. whereas control values of carbofuran were approximately from 5 to 20% in all test soils having different organic matter contents. These results indicate that carbofuran has more vertical migration effect than ethoprophos. Persistence of the two neniaticides was also decreased with increasing soil organic matter content. Half life of carbofuran was 2-3 weeks in soil containing 0.4% organic matter, whereas it was found to be I week in soils containning 0.8 ant1 1.6% of organic matter. On the other hand. activity of ethoprophos was reduced to half in 3-3 weeks and in 2-3 weeks in soil containing 0.4 and 0.8%, and 1.6%) of organic matter, respectively. However, no activity of the both nernaticides was found in soil containing 3.2% of organic matter.

• BMB Reports
• /
• v.44 no.1
• /
• pp.1-10
• /
• 2011

Inter-cellular communication via diffusible small molecules is a defining character not only of multicellular forms of life but also of single-celled organisms. A large number of bacterial genes are regulated by the change of chemical milieu mediated by the local population density of its own species or others. The cell density-dependent "autoinducer" molecules regulate the expression of those genes involved in genetic competence, biofilm formation and persistence, virulence, sporulation, bioluminescence, antibiotic production, and many others. Recent innovations in recombinant DNA technology and micro-/nano-fluidics systems render the genetic circuitry responsible for cell-to-cell communication feasible to and malleable via synthetic biological approaches. Here we review the current understanding of the molecular biology of bacterial intercellular communication and the novel experimental protocols and platforms used to investigate this phenomenon. A particular emphasis is given to the genetic regulatory circuits that provide the standard building blocks which constitute the syntax of the biochemical communication network. Thus, this review gives focus to the engineering principles necessary for rewiring bacterial chemo-communication for various applications, ranging from population-level gene expression control to the study of host-pathogen interactions.

• KIEAE Journal
• /
• v.12 no.6
• /
• pp.55-62
• /
• 2012

Recently, public concerns about renewal of abandoned sites centered in Industrial Heritage are increasing in Korea. For this reason, the purpose of this study was to analyze characteristics of renewal methods of public space using abandoned sites. To achieve the proposed goal, the first part of this study focused on showing public properties involved in the fundamental reasons for the birth and life(persistence) of abandoned sites, which testified that they should be regenerated into public spaces to maximize the potentiality of abandoned sites. Upon preliminary analysis, main values of public space renewal were drawn through the analysis of the selection criteria, type of information about selected works, and classification system in 'The European Prize for Urban Public Space' hosted by the CCCB(Centre of Contemporary Culture of Barcelona). Then, 427 projects participating in this prize from 2000 to 2010 were analyzed to identify characteristics of each level (site-program, process and space). Although this study was limited to the analysis of European projects, it proved that citizens' lives and activities should be involved in projecting-practicing-designing of renewal project using abandoned sites to create a successful public space.

• Clinical and Experimental Pediatrics
• /
• v.62 no.6
• /
• pp.199-205
• /
• 2019

Although Mycoplasma pneumoniae pneumonia (MPP) has been generally susceptible to macrolides, the emergence of macrolide-resistant MPP (MRMP) has made its treatment challenging. MRMP rapidly spread after the 2000s, especially in East Asia. MRMP is more common in children and adolescents than in adults, which is likely related to the frequent use of macrolides for treating M. pneumoniae infections in children. MRMP is unlikely to be related to clinical, laboratory, or radiological severity, although it likely prolongs the persistence of symptoms and the length of hospital stay. Thereby, it causes an increased burden of the disease and poor quality of life for the patient as well as a societal socioeconomic burden. To date, the only alternative treatments for MRMP are secondary antimicrobials such as tetracyclines (TCs) or fluoroquinolones (FQs) or systemic corticosteroids; however, the former are contraindicated in children because of concerns about potential adverse events (i.e., tooth discoloration or tendinopathy). A few guidelines recommended TCs or FQs as the second-line drug of choice for treating MRMP. However, there have been no evidence-based guidelines. Furthermore, safety issues have not yet been resolved. Therefore, this article aimed to review the benefits and risks of therapeutic alternatives for treating MRMP in children and review the recommendations of international or regional guidelines and specific considerations for their practical application.

• Korean Journal of Child Studies
• /
• v.37 no.2
• /
• pp.57-77
• /
• 2016

Objective: This study examined the influences of general characteristics, temperament, social support, and emotional regulation on the positive psychological capital of adolescents. The data were collected from 672 middle school students living in Gwangju. Methods: Data were analyzed using Pearson correlation analyses and hierarchical regression analyses. Results: Temperament best explained positive psychological capital. In terms of individual factors, self-emotion regulation had the greatest effect on positive psychological capital, followed by friend support, persistence temperament, others emotion regulation, academic achievement, harm avoidance temperament, gender, and family support, in that order. Conclusion: The results for the effects of temperament, social support and emotion regulation on adolescent's positive psychological capital highlights the important roles played by the temperament, social support, and emotion regulation in improving an adolescent's positive psychological capital. The study contributes to the literature by proving fundamental insights into an adolescent's psychological strengths and higher quality of life.