• Title/Summary/Keyword: Peronospora destructor

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Phylogenetic Analysis of Downy Mildew Caused by Peronospora destructor and a Method of Detection by PCR (양파 노균병균 Peronospora destructor의 분자계통학적 유연관계 분석과 PCR 검출기술 개발)

  • Back, Chang-Gi;Hwang, Sun-Kyung;Park, Mi jeong;Kwon, Young-Seok;Jung, Hee-Young;Park, Jong-Han
    • The Korean Journal of Mycology
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    • v.45 no.4
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    • pp.386-393
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    • 2017
  • Onion downy mildew, caused by Peronospora destructor, is a major disease in onion cultivation areas in Korea. The causal fungi were collected and analyzed based on sequence similarity and molecular phylogenetic relationships of multi-gene sequences, including the internal transcribed spacer (ITS) region. All isolates from Changnyeong-gun, Hamyang-gun, and Hapcheon-gun in Gyeongnam province, and Muan-gun, Haenam-gun, and Sinan-gun in Jeonnam province were identical in the four types of gene sequences, indicating they were genetically the same strains. In this study, a PCR method was developed based on the ITS gene sequences to amplify the specific DNA fragment for P. destructor only. The detection limit of was total genomic DNA of the P. destructor and the plant $0.7ng/{\mu}L$. Therefore, the developed PCR method could be used to detect P. destructor effectively from symptomless onion leaves.

Control of Diseases and Insects for Pesticide-free Cultivation of Leafy Vegetables (엽채류 유기재배의 병해충 관리)

  • Seo, Young-Ho;Cho, Byoung-Ouk;Choi, Jun-Keun;Kang, An-Seok;Jeong, Byung-Chan
    • Korean Journal of Organic Agriculture
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    • v.17 no.2
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    • pp.253-264
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    • 2009
  • This study was conducted to establish agricultural practices to control diseases and insects for chemical pesticide-free cultivation of leafy vegetables. Two diseases, gray mold(Botrytis cinerea) and soft rot(Erwinia carotovora), on lettuce were reduced by controlling temperature and humidity using air-circulation fan. The aphidophagous lady beetle(Harmonia axyridis) and primary parasitoids(Aphidius colemani) showed activity to control aphids density on Chinese cabbage. Co-application of cooking oil and yolk mixture (COY) and BT(Bacillus thuringiensis) decreased diseases including soft rot(Erwinia carotovora), downy mildew(Peronospora brassicae Gaumann), and powdery mildew(Eryslphe polygoni), and insects such as diamondback moth(Plutella xylostella) and beet armyworm(Spodoptera exigua Hubner). Neem extract treatment reduced downy mildew(Peronospora destructor) on Welsh onion.

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Control Efficacy of Several Fungicides against Downy Mildew of Onion at Nursery Seedling Stage (육묘상 양파 노균병에 대한 몇 가지 살균제의 방제 효과)

  • Mo, Chung-Yong;Lee, Jang Hoon;Ko, Suk-Juo;Yang, Kwang-Yeol
    • Research in Plant Disease
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    • v.22 no.3
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    • pp.208-212
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    • 2016
  • During the nursery seedlings stage at Muan in 2013, moisture and temperature conditions were favorable for the occurrence of onion downy mildew. Several fungicides were evaluated for controlling onion downy mildew at the stage of seedling. The disease incidence of the untreated control was 51.1%, which was sufficient to evaluate the fungicide efficacy for the control of onion downy mildew. Incidence at the mancozeb treatment (MAN treatment) was 4.4% and it was the highest control value of 91.4%. Incidence at the various fungicides treatment (MIX treatment) consisted of amectotradin plus dimethomorph, floupicolide plus propamocarb HCl, or dimethomorph plus pyraclostrobin was 10.0%, and the control value of the MIX treatment was 80.4%. Among the tested fungicides, amectotradin plus dimethomorph (AME plus DIM treatment) was selected and treated. Incidence of AME plus DIM treatment was 12.2%. There was statistically significant difference among the three different treatments and the untreated control. These results suggest that the application of fungicides on onion nursery seedlings was effective to control the onion downy mildew.