• Title/Summary/Keyword: Perkinsus sp.

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Parasitism of the protozoan Perkinsus atlanticus in Manila clams, Ruditapes philippinarum, in Gomso Bay (Korea) and Ariake Bay (Japan)

  • Park, Kyung-Il;Choi, Kwang-Sik;Ngo, Thao T.T.;Tsutsumi, Hiro;Hong, Jae-Sang
    • Proceedings of the Korean Aquaculture Society Conference
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    • 2004.05a
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    • pp.513-513
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    • 2004

  • Manila clam, Ruditapes philippinarum, is commercially and ecologically important marine bivalve in Korea and japan. However, clam landings in the two countries have dramatically declined since the 1980-1990's. In the present study, the protozoan parasite, Perkinsus sp., lectin (host's defense-related glycoprotein) and histopathological features were investigated in Manila clams collected from Gomso Bay in Korea and Ariake Bay in japan (one of the largest clam beds in each country) during summer and fall, 2002-2003. DNA sequences of non-transcribe spacer (NTS), internal transcribed space. (ITS) and 5.85 rRNA of Perkinsus sp. were identical to those of P. atlanticus that was reported in Europe and Korea. For diagnosis of Perkinsus, the fluid thioglycollate medium (FTM) and the 2 M NaOH lysis methods were used. Prevalence of the parasite varied from 92.5-98.7% in Gomso Bay and 35.5-37.9% in Ariake Bay. Infection intensity, in terms of the number of Perkinsuscells per gram tissue wet weight, in the clams of Gomso Bay in fall 2002 averaged 1,010,077-470,937 recording approximately100 times higher than that of Ariake Bay, and these were twice higher than those of summer samples in each location. Mean hemagglutination titer of the clams from Gomso Bay was approximately 60-folds higher than that of clams from Ariake Bay in 2002. In histological preparation of the clams from Gomso Bay in 2002, trophozoites of P. atlanticus were in groups and resulted in severe inflammatory response of host clam. Prevalence of the trematod, Cercaria tapes-like in the clams of Gomso Bay and Ariake Bay were 8.8 % and 10.5% respectively. In conclusion, the clams from Gomso Bay showed more severe pathologic symptoms and higher immune response than those of the clams from Ariake Bay.

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경남 해안 바지락에서 출현한 바지락포자충, Perkinsus sp.의 보고

  • 박경일;최광식;최진우;허성범
    • Proceedings of the Korean Society of Fisheries Technology Conference
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    • 2000.05a
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    • pp.503-504
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    • 2000

  • 바지락포자충 Perkinsus는 미국과 유럽 등지에서 굴과 바지락에 기생하며 고수온기 숙주의 대량 폐사를 유발하는 원생동물로 이에 대한 연구가 활발하게 진행중인 패류질병이다. Choi 와 Park (1997)은 1990년대 초반부터 나타난 여름철 바지락 대량 폐사 원인 중의 하나로 바지락포자충을 폐사 원인 생물로 추정한 바 있다. 이 연구는 1997년부터 진행중인 우리 나라 전 해역에 대한 바지락포자충 감염현장 조사의 일환으로, 경남지역에 분포하는 바지락의 바지락포자충 감염에 대한 조사 보고이다. (중략)

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