• Animal Systematics, Evolution and Diversity
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• v.8 no.1
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• pp.1-10
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• 1992

A nereid worm , Perineresi aibuhitensis has been frequently mis-identified in Korea as P.vancaurica tetradentata or Neanthes virens due to the wide variation in the number and arrangement of paragnaths on its proboscis. In this paper we re-examined ca. 50 worms collected in three intertidal mud flats of the western coast of Korea, and identified them to be P.aibuhitensis , a new record in Korea. We also described the hteronereid of this species and its biogeographical range.

• Journal of Aquaculture
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• v.10 no.1
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• pp.17-24
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• 1997

In order to understand the spawning and larval development of Perinereis aibuhitensis for the effective seedling production, the influence of water temperature on spawning induction, egg and larval development of the worm were investigated from October 30, 1989 to October 30, 1990. Main spawning period was from July through September, and average diameter of mature eggs was $220\mu m$. The relationship between the adult weight (Wt) and the number of spawned eggs (NS) was given as follws : NS=48635.589Wt1.3044 (r=0.8572). Adult males and females died immediately after spawning. Trochophore larvae developed 12 hours after fertilization, and hatched out after 56 hours.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.30 no.4
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• pp.634-639
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• 1997

In order to obtain the basic knowledges for the effective seed production of Perinereis aibuhitensis, sediment preference, burrow shape and behavior with in burrow were investigated in the laboratory. The highest value in both sediment preference and survival rate of P. aibuhitensis were shown at fine sand below 0.10 mm in the mean diameter. The worm made various types of burrows, such as J, L, O, Y and I shapes. Generally, only one individual inhabits in a burrow with head-up, but when excreting, it positions up-side down.

• Journal of Microbiology and Biotechnology
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• v.20 no.8
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• pp.1243-1250
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• 2010

A cDNA encoding a cysteine protease inhibitor (CPI) was isolated from the cDNA library of clamworm Perinereis aibuhitensis Grube. The deduced amino acid sequence analysis showed that the protein had 51%, 48%, and 48% identity with Zgc:153129 from Danio rerio, cystatin B from Theromyzon tessulatum, and the ChainA, stefin B tetramer from Homo sapiens, respectively. The gene was cloned into the intracellular expression vector pET-15b and expressed in Escherichia coli. The recombinant CPI (PA-CPI) was purified by affinity chromatography on Ni-charged resin and ion-exchange chromatography on DEAE-Sepharose FF. The relative molecular mass of PA-CPI was 16 kDa as deduced by SDS-PAGE. Activity analysis showed that the recombinant protein could inhibit the proteolytic activity of papain. A constitutive and secretive expression vector was also constructed, and the cDNA encoding CPI was subcloned into the vector for extracellular expression. Western blotting analysis results showed that the PA-CPI was secreted into the medium. Bioassay demonstrated that E. coli DH5${\alpha}$ harboring pUC18ompAcat-CPI showed a significant difference in mortality to the Asian longhorned beetle Anoplophora glabripennis compared with untransformed E. coli DH5${\alpha}$ and control.