• 생명과학회지
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• 제20권4호
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• pp.467-473
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• 2010

갱글리오시드는 포유동물 세포막의 중요한 구성요소로서 세포와 세포 혹은 세포와 단백질간의 상호작용을 포함한 다양한 면역학적 역할을 수행하고 있다. 이 연구는 NIH-미니돼지의 간과 심장을 인간에게 이식할려고 할 때 예측되어지는 거부 반응과 관련된 구성성분들 중 시알산을 함유하고 있는 스핑고당지질인 갱글리오시드에 대해 조사하였다. 얇은막크로마토그래피와 면역조직화학적분석을 실시한 결과 NIH-미니돼지의 간은 갱글리오시드의 발현이 심장보다 높게 나타났다. 갱글리오시드 GD3, GD1a, GD1b, GT1b는 간과 심장의 두 기관에서 발견되었다. 그러나 GQ1b는 간에서만 발견되었고 심장에서는 검출되지 않았다. 이러한 결과는 갱글리오시드의 발현양상은 간과 심장에서 조직특이적이라는 것을 의미한다. 한편, GM3를 포함한 다른 갱글리오 시리즈인 갱글리오시드들은 NIH-미니돼지의 간과 심장에서 검출되어지지 않았다. 이와 같은 연구결과로부터 갱글리오시드는 미니돼지의 장기중 특히, 간과 심장의 이종장기이식과 관련된 면역거부반응에서 어떤 역할을 수행하고 있다고 여겨진다.

• 수면정신생리
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• 제5권2호
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• pp.119-133
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• 1998

Attentional processes facilitate cognitive and behavioral performance in several ways. Attention serves to reduce the amount of information to receive. Attention enables humans to direct themselves to appropriate aspects of external environmental events and internal operations. Attention facilitates the selection of salient information and the allocation of cognitive processing appropriate to that information. Attention is not a unitary process that can be localized to a single neuroanatomical region. Before the cortical registration of sensory information, activation of important subcortical structures occurs, which is called as an orienting response. Once sensory information reaches the sensory cortex, a large number of perceptual processes occur, which provide various levels of perceptual resolution of the critical features of the stimuli. After this preattentional processing, information is integrated within higher cortical(heteromodal) systems in inferior parietal and temporal lobes. At this stage, the processing characteristics can be modified, and the biases of the system have a direct impact on attentional selection. Information flow has been traced through sensory analysis to a processing stage that enables the new information to be focused and modified in relation to preexisting biases. The limbic and paralimbic system play significant roles in modulating attentional response. It is labeled with affective salience and is integrated according to ongoing pressures from the motivational drive system of the hypothalamus. The salience of information greatly influences the allocation of attention. The frontal lobe operate response selection system with a reciprocal interaction with both the attention system of the parietal lobe and the limbic system. In this attentional process, the search with the spatial field is organized and a sequence of attentional responses is generated. Affective, motivational and appectitive impulses from limbic system and hypothalamus trigger response intention, preparation, planning, initiation and control of frontal lobe on this process. The reticular system, which produces ascending activation, catalyzes the overall system and increases attentional capacity. Also additional energetic pressures are created by the hypothalamus. As psychophysiological measurement, skin conductance, pupil diameter, muscle tension, heart rate, alpha wave of EEG can be used. Event related potentials also provide physiological evidence of attention during information process. NI component appears to be an electrophysiological index of selective attention. P3 response is developed during the attention related to stimulus discrimination, evaluation and response.

• 해양정책연구
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• 제33권2호
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• pp.83-119
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• 2018

Under the Korean Marine Environment Management Act (hereunder, the KMEMA), the duty to arrange spill clean-up equipments, including spill clean-up vessels, required by the article 67 of the KMEMA is in essence the provision of public goods since it is a precautionary or preventive measure for the efficient performance of oil spill cleaning up. Also the obligation to control marine pollution and the duty to arrange of anti-pollution measures imposed on polluters is the obligation imposed by the public law in accordance with "the Polluter-Pays Principle". Therefore, the execution of such obligations shall be accompanied by the minimum legal and institutional arrangements. On the other hand, judging whether to form the roles of the public and private sectors in providing public goods is basically a matter of policy decision. However, even if the private sector is allowed to participate in the provision of public goods, it is imperative that a minimum requirement be provided to secure the public interest. Although major countries allows polluters to conclude a preliminary contract with a civil anti-pollution management company, these civilian institutions are in principle constituted by the owners of oil storage facilities. Additionally it is worth noting that it operates as a non-profit organization. In particular, if the practitioner performs pollution control for commercial purposes, their profitability may depend on the size of the pollution, the period spent on pollution control, the size of the equipment and manpower mobilized in the pollution control, and so on. Considering the above problems, caution should be taken to allow marine environmental management companies to be un-limitedly entrusted with the responsibility of arranging measures such as pollution control. In order to allow the marine environmental management contractor to be entrusted for the assignment of duty to protect the marine environment, the marine environment management business should be expanded so that the marine pollutant management capacity satisfies the statutory control capacity. For this purpose, it is necessary to manage and supervise the maintenance and improvement of the control capability of the marine environment management business. It is also necessary to discuss the introduction of the grading system for the control ability of the civil control companies alike in major countries.

• 의료법학
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• 제9권1호
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• pp.385-411
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• 2008

The goal of medicine is to contribute to promoting national health by preventing diseases and providing treatment. The scope of modern medicine isn't merely confined to disease testing, treatment and prevention in accordance to that, and making experiments by using the human body is widespread. The advance in modern medicine has made a great contribution to valuing human dignity and actualizing a manly life, but there is a problem that has still nagged modern medicine: treatment and healing for terminal patients including cancer patients. In advanced countries, pain care and hospice medicine are already universal. Offering a helping hand for terminal patients to lead a less painful and more manly life from diverse angles instead of merely focusing on treatment is called the very hospice medicine. That is a comprehensive package of medical services to take care of death-facing terminal patients and their families with affection. That is providing physical, mental and social support for the patients to pass away in peace after living a dignified and decent life, and that is comforting their bereaved families. The National Hospice Organization of the United States provides terminal patients and their families with sustained hospital care and home care in a move to lend assistance to them. In our country, however, tertiary medical institutions simply provide medical care for terminal patients to extend their lives, and there are few institutional efforts to help them. Hospice medicine is offered mostly in our country by non- professionals including doctors, nurses, social workers, pastors or physical therapists. Terminal patients' needs cannot be satisfied in the same manner as those of other patients, and it's needed to take a different approach to their treatment as well. Nevertheless, the focus of medical care is still placed on treatment only, which should be taken seriously. Ministry for Health, Welfare & Family Affairs and Health Insurance Review & Assessment Service held a public hearing on May 21, 2008, on the cost of hospice care, quality control and demonstration project to gather extensive opinions from the academic community, experts and consumer groups to draw up plans about manpower supply, facilities and demonstration project, but the institutions are not going to work on hospice education, securement of facilities and relevant legislation. In 2002, Ministry for Health, Welfare & Family Affairs made an official announcement to introduce a hospice nurse system to nurture nurse specialists in this area. That ministry legislated for the qualifications of advanced nurse practitioner and a hospice nurse system(Article 24 and 2 in Enforcement Regulations for the Medical Law), but few specific plans are under way to carry out the regulations. It's well known that the medical law defines a nurse as a professional health care worker, and there is a move to draw a line between the responsibilities of doctors and those of nurses in association with medical errors. Specifically, the roles of professional hospice are increasingly expected to be accentuated in conjunction with treatment for terminal patients, and it seems that delving into possible problems with the job performance of nurses and coming up with workable countermeasures are what scholars of conscience should do in an effort to contribute to the development of medicine and the realization of a dignified and manly life.

• 한국안전학회지
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• 제32권5호
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• pp.168-178
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• 2017

In order to prevent major and chemical accidents, some of the plants which would like to install and operate hazard chemicals handling facilities must submit Off-site Consequence Analysis due to recent arisen leak accidents since 2015. A lot of chemical industrials choose gas detectors as mitigation equipment to early detect gas vapor. The way of placement of gas detectors has two methods; Code-based Design(CBD) and Performance-based Design. The CBD has principles for gas detectors to be installed with consideration for the place that is expected to accumulate gas, and the leak locations according to legal standards and technical guidelines, and has a possibility to be unable to detect by these rules to locate gas detectors by vapor density information. The PBD has two methods; a Geographic Method and Scenario based Method. The Scenario-based Method has been suggested to make up for the Geographic Coverage Method. This Scenario-based Method draw the best optimum placement of gas detectors by considering leak locations, leak speed information, leak directions and etc. However, the domestic placement guidelines just refers to the CBD. Therefore, this study is to compare existing placement location of gas detectors by the domestic CBD with placement locations, coverages and the number of gas detectors in accordance with the Scenario-based Method. Also this study has measures for early detecting interest of Vapor Cloud and suitable placement of gas detectors to prevent chemical accidents. The Phast software was selected to simulate vapor cloud dispersion to predict the consequence. There are two cases; an accident hole size of leak(8 mm) from API which is the highst accident hole size less than 24.5 mm, and a normal leak hole size from KOSHA Guide (1.8 mm). Detect3D was also selected to locate gas detectors efficiently and compare CBD results and PBD results. Currently, domestic methods of gas detectors do not consider any risk, but just depend on domestic code methods which lead to placement of gas detectors not to make personnels recognize tolerable or intolerable risks. The results of the Scenario-based Method, however, analyze the leak estimated range by simulating leak dispersion, and then it is able to tell tolerable risks. Thus it is considered that individuals will be able to place gas detectors reasonably by making objectives and roles flexibly according to situations in a specific plant.

• 디지털융복합연구
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• 제18권3호
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• pp.383-393
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• 2020

본 연구는 2017년부터 2018년까지, 2년간의 중증장애인생산품 생산시설 현황을 조사하고, 이전 선행 연구와 비교하여 중증장애인생산품 우선구매제도의 개선방안을 제시하는 데 목적이 있다. 2018년 6월 기준 544곳 중 267곳을 대상으로 조사하여 결과를 분석하였다. 연구 결과, 첫째, 중증장애근로자는 평균 16.51명이며, 전체 근로자현황에서 2017년보다 2018년에 심사기준상 전체 근로자, 직접생산에 참여하는 장애인근로자, 비장애근로자가 대부분 증가추세를 보였다. 둘째, 직접생산에 참여하는 장애인근로자는 월평균 급여가 2017년 790,977원에서 2018년 829,810원으로 다소 증가추세를 보였다. 셋째, 생산시설 매출현황에서 지정 전후의 매출 변화를 분석한 결과 전반적으로 지정받기 전보다 지정받은 해, 지정받은 후에 증가한 것으로 나타났다. 개선방안으로는 우선구매율 향상, 생산시설과 구매 공공기관에 대한 지원, 업무수행기관의 역할 분담, 중증장애인생산품 우선구매촉진위원회 역할 명확화, 최저임금적용제외 제도에 대한 보완이 필요하다.

• Nutrition Research and Practice
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• 제5권5호
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• pp.396-403
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• 2011

Ceramides (Cer) comprise the major constituent of sphingolipids in the epidermis and are known to play diverse roles in the outermost layers of the skin including water retention and provision of a physical barrier. In addition, they can be hydrolyzed into free sphingoid bases such as $C_{18}$ sphingosine (SO) and $C_{18}$ sphinganine (SA) or can be further metabolized to $C_{18}$ So-1-phosphate (S1P) and $C_{18}$ Sa-1-phosphate (Sa1P) in keratinocytes. The significance of ceramide metabolites emerged from studies reporting altered levels of SO and SA in skin disorders and the role of S1P and Sa1P as signaling lipids. However, the overall metabolism of sphingoid bases and their phosphates during keratinocyte differentiation remains not fully understood. Therefore, in this study, we analyzed these Cer metabolites in the process of keratinocyte differentiation. Three distinct keratinocyte differentiation stages were prepared using 0.07 mM calcium (Ca$^{2+}$) (proliferation stage), 1.2 mM Ca$^{2+}$ (early differentiation stage) in serum-free medium, or serum-containing medium with vitamin C (50 ${\mu}L$/mL) (late differentiation stage). Serum-containing medium was also used to determine whether vitamin C increases the concentrations of sphingoid bases and their phosphates. The production of sphingoid bases and their phosphates after hydrolysis by alkaline phosphatase was determined using high-performance liquid chromatography. Compared to cells treated with 0.07 mM Ca$^{2+}$, levels of SO, SA, S1P, and SA1P were not altered after treatment with 1.2 mM Ca$^{2+}$. However, in keratinocytes cultured in serum-containing medium with vitamin C, levels of SO, SA, S1P, and SA1P were dramatically higher than those in 0.07- and l.2-mM Ca$^{2+}$-treated cells; however, compared to serum-containing medium alone, vitamin C did not significantly enhance their production. Taken together, we demonstrate that late differentiation induced by vitamin C and serum was accompanied by dramatic increases in the concentration of sphingoid bases and their phosphates, although vitamin C alone had no effect on their production.

• 기독교교육논총
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• 제61권
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• pp.81-107
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• 2020

이 논문은 관찰학습에서 나타나고 있는 반두라의 대리강화에 대한 이론과 효과들을 살펴보고, 그 개념을 기독교교육에서 행해지고 있는 영적 롤 모델링 전략에 적용한 연구이다. 이를 위해 먼저 반두라가 소개하고 있는 대리강화란 무엇이며, 관찰학습에서 대리강화가 어떻게 발생하는가 그리고 대리강화가 관찰자의 행동 변화에 어떠한 영향을 미치는가에 대하여 살펴보았다. 특별히 반두라는 관찰학습과 모방 혹은 반모방 행동 안에서 일어나는 학습과정에 대하여 개념화하였다. 이를 바탕으로 하여 반두라는 관찰학습에서 일어나는 대리강화의 과정을 4단계(집중, 기억, 행동재생, 그리고 동기화)로 분류하여 설명한다. 그뿐만이 아니라. 대리강화의 효과에 대해 3가지(관찰학습효과, 억제-탈억제효과, 유발효과)를 제시하였다. 이러한 반두라의 대리강화 이론과 그 효과를 바탕으로 하여 기독교교육에서 논의되고 있는 예수님 닮아가기 혹은 제자훈련 안에서의 영적 롤 모델링 전략을 재평가했다. 특별히 성경에서 사용하고 있는 모방에 대한 개념을 반두라의 대리강화 이론과 비교하면서 재해석하였고, 사도 바울이 제시하고 있는 영적 롤 모델의 역할을 대리강화의 효과라는 차원에서 재점검했다. 그 결과 2가지 차원에서의 영적 롤 모델 전략을 발견하였다. 즉 바울의 영적 롤 모델 역할은 긍정적인 차원에서 대리강화로써 신자로 하여금 기대되는 행동을 실행할 수 있도록 도와주는 하나의 본보기가 된다. 반면에 바울의 명령은 신자들에게 명확한 탈억제 효과를 불러일으키는 부정적인 차원에서의 대리강화로 사용되고 있음을 발견하였다. 그리고 마지막으로 관찰학습에 대한 연구들과 성경적 영적 롤 모델링 전략을 서로 연관시킴으로써 반두라의 대리강화가 기독교교육에 미칠 가능성을 함의하였다.

• 트랜스-
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• 제2권
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• pp.161-190
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• 2017

시대가 발전함에 따라 예술의 창조적 변화는 다양해지는 요구들을 수용하려는 시도들로 이루어지는데, 이러한 시도들은 예술의 장르적 벽을 허물고 다양한 요소들이 무용 작품 안에서 활용된 오브제의 형태로 표현되고 오늘날 오브제 특성이 표현되지 않은 작품은 찾아보기 힘들다. 본 연구는 오브제를 총체적인 관점에서 하나의 체계로 이해하고자 하며 오브제의 미학적 논의 과정을 고찰함으로써 오브제의 표현과 특성이 어떻게 무용 작품 안에서 어떠한 영향을 미치고 어떠한 역할을 히는지 분석해보고자 한다. 또한 오브제들이 무용 작품 안에서 작품을 어떻게 자극하고 활성화시키는지 표현 특성을 연구함으로써, 본 연구가 무용예술 작품이 발전할 수 있는 중요한 역할을 할 것이라고 본다. 윌리엄 포사이드(William Forsythe) 작품 중 오브제의 표현 특성이 명백하게 드러나는 작품 (2006), (2009), (2009)를 선정하였다. 작품에 나타난 오브제 표현 특성은 다양한 매체의 활용과 신체 행위, 비물질적 요소까지 활용하면서도 무용의 본질은 잃지 않고 자신만의 독창성을 극대화하였으며 무용 공연 표현의 확장을 가져왔다. 그 결과 기존의 한계를 극복할 수 있는 다양한 방법과 표현의 확장을 가져다주는 이론을 제공하고 관객과의 거리를 좁히며 관객과의 새로운 소통 방법을 제시하고 있다.

• 한국진공학회:학술대회논문집
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• 한국진공학회 2013년도 제44회 동계 정기학술대회 초록집
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• pp.108-109
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• 2013

Mitochondria play key roles in the production of cell's energy. Their dominant function is the synthesis of adenosine 5'-triphosphate (ATP) from adenosine diphosphate (ADP) and phosphate (Pi) through the oxidative phosphorylation. Evaluation of drug-induced mitochondrial toxicity has become increasingly important since mitochondrial dysfunction has recently been implicated in numerous diseases including cancer and diabetes mellitus. Mitochondrial functions have been monitored via oxygen consumption, mitochondrial membrane potential, and more importantly via ATP synthesis since ATP synthesis is the most essential function of mitochondria. Various analytical methods have been employed to investigate ATP synthesis in mitochondria, including high performance liquid chromatography (HPLC), bioluminescence technique, and pH measurement. However, most of these methods are based on destructive analysis or indirect monitoring through the enzymatic reaction. Infrared absorption spectroscopy (IRAS) is one of the useful techniques for real-time, label-free, and direct monitoring of biological reactions [1,2]. However, the strong water absorption requires very short path length in the order of several micrometers. Transmission measurements with thin path length are not suitable for mitochondrial assays because solution handlings necessary for evaluating mitochondrial toxicity, such as rapid mixing of drugs and oxygen supply, are difficult in such a narrow space. On the other hand, IRAS in the multiple internal reflection (MIR) geometry provides an ideal optical configuration to combine solution handling and aqueous-phase measurement. We have recently reportedon a real-time monitoring of drug-induced necrotic and apoptotic cell death using MIR-IRAS [3,4]. Clear discrimination between viable and damaged cells has been demonstrated, showing a promise as a label-free and real-time detection for cell-based assays. In the present study, we have applied our MIR-IRAS system to mitochondria-based assays by monitoring ATP synthesis in isolated mitochondria from rat livers. Mitochondrial ATP synthesis and hydrolysis were in situ monitored with MIR-IRAS, while dissolved oxygen level and solution pH were simultaneously monitored with O2 and pH electrodes, respectively. It is demonstrated that ATP synthesis and hydrolysis can be monitored by the IR spectral changes in phosphate groups in adenine nucleotides and MIR-IRAS is useful for evaluating time-dependent drug effects of mitochondrial toxicants.