• The Korean Journal of Physiology and Pharmacology
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• 제20권4호
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• pp.399-406
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• 2016

Early life neuronal exposure to environmental toxicants has been suggested to be an important etiology of neurodegenerative disease development. Perfluorohexanesulfonate (PFHxS), one of the major perfluoroalkyl compounds, is widely distributed environmental contaminants. We have reported that PFHxS induces neuronal apoptosis via ERK-mediated pathway. Imperatorin is a furanocoumarin found in various edible plants and has a wide range of pharmacological effects including neuroprotection. In this study, the effects of imperatorin on PFHxS-induced neuronal apoptosis and the underlying mechanisms are examined using cerebellar granule cells (CGC). CGC were isolated from seven-day old rats and were grown in culture for seven days. Caspase-3 activity and TUNEL staining were used to determine neuronal apoptosis. PFHxS-induced apoptosis of CGC was significantly reduced by imperatorin and PD98059, an ERK pathway inhibitor. PFHxS induced a persistent increase in intracellular calcium, which was significantly blocked by imperatorin, NMDA receptor antagonist, MK801 and the L-type voltage-dependent calcium channel blockers, diltiazem and nifedipine. The activation of caspase-3 by PFHxS was also inhibited by MK801, diltiazem and nifedipine. PFHxS-increased ERK activation was inhibited by imperatorin, MK801, diltiazem and nifedipine. Taken together, imperatorin protects CGC against PFHxS-induced apoptosis via inhibition of NMDA receptor/intracellular calcium-mediated ERK pathway.

• 한국지반환경공학회 논문집
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• 제18권11호
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• pp.5-12
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• 2017

과불화 화합물(perfluorinated compounds, PFCs)은 중요한 환경이슈이다. 하지만 한국의 동 서해안 하구의 해수에서 PFCs 모니터링에 관한 연구는 거의 알려져 있지 않다. 이 연구는 동 서해안 하구의 해수에서 PFCs 모니터링 결과를 보여준다. 이 연구에서 선정된 10종의 PFCs 중 동해안에서 PFOA, PFOS, PFBS, PFHxS의 농도는 각각 9.6-50.7ng/L(평균: $26.14{\pm}12.66ng/L$), 13.79-44.58ng/L($27.95{\pm}11.41ng/L$), LOQ-2.6ng/L($0.96{\pm}1.15ng/L$), 2.95-11.05ng/L($4.25{\pm}2.57ng/L$)이었다. 서해안에서 PFOA, PFOS, PFBS, PFHxS의 농도는 각각 8.97-22.53ng/L($14.47{\pm}4.25ng/L$), 27.66-51.71ng/L($36.27{\pm}7.79ng/L$), LOQ-2.27ng/L($1.63{\pm}0.93ng/L$), 3.0-7.66ng/L($4.27{\pm}1.49ng/L$)이었다. 다른 PFCs 화학종은 LOQ 이하로 나타났다. 이 연구결과는 우리나라 동 서해안 하구지역에서 환경오염을 평가하기 위한 PFCs의 분포 양상을 제공한다.