• Title/Summary/Keyword: Pepsin digestibility

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Evaluation on Feed-Nutritional Change of Food Waste According to Different Processing Methods and Trouble-shooting Strategy (음식물쓰레기의 가공처리방법별 사료영양소 함량 변화 평가 및 문제점 개선 방안)

  • Jee, K.S.;Baik, Y.H.;Kwak, W.S.
    • Journal of Animal Science and Technology
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    • v.47 no.4
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    • pp.513-524
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    • 2005
  • This study was conducted to introduce recycling procedures of food waste(FW) as feed according to the dehydration, semi-dehydration fermentation and liquid fermentation methods through the on-site survey of companies related, to trace physico-chemical components and nutritional losses depending upon the processing stage for each method and finally to suggest more desirable methodology for the efficient utilization of FW as animal feed. For the dehydration method, dewatering of FW alone reduced(P<0.05) moisture(approximately 10%) and ether extract contents and increased(P<0.05) fiber contents. Dewatering and subsequent dehydration of FW decreased(P<0.05) contents of ether extract, limiting amino acids such as lysine, methionine and histidine, pepsin digestibility of protein by half, and NaCl content by 40%, increased(P<0.05) contents of fiber, crude ash, Ca and P, and did not alter(P>0.05) pH. The semi-dehydration fermentation method of FW did not affect(P>0.05) the chemical components, pepsin digestibility of protein, pH and NaCl content. For the liquid fermentation method, pasteurization and fermentation of FW decreased(P<0.05) contents of dry matter, ether extract, crude fiber, lysine and NaCl; however, it did not affect(P>0.05) other chemical components, pepsin digestibility of protein and pH. Among the processing methods, nutrient losses were highest for the dehydration method(25% of metabolizable energy loss, 12% of organic matter loss) and little for the semi-dehydration and liquid fermentation methods. The on-site survey of companies related revealed that the existence of foreign materials in FW products were problematic for all the three companies surveyed, thus it was necessary to develop a more efficient screener. Before feeding FW-containing diets to pigs, high quality of protein and energy feedstuffs needed to be fortified for the dehydration method. For the semi-dehydration fermentation method, the scientific diet formulation technology was required at the initial mixing stage. For the liquid fermentation method, possibly most energetic and proteinaceous feeds needed to be supplemented for the normal animal growth.

Applications of Near Infrared Reflectance Spectroscopy(NIRS) in Forage Evaluation (조사료 가치 평가를 위한 근적외선 분광법(NIRS)의 활용)

  • 박형수;이종경;이효원
    • Journal of The Korean Society of Grassland and Forage Science
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    • v.24 no.1
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    • pp.81-90
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    • 2004
  • Farmers need timely information on the nutritional status of their animals and the nutritive value of pastures and supplementary feeds if they are to apply successfully this existing nutritional information. Near infrared reflectance(NIR) spectroscopy has been used over the last forty years to analyse accurately protein, fiber, and other organic components in animal foods. NIR spectroscopy is a rapid, non-destructive, and non-polluting technology. When properly calibrated, NIR spectroscopy is used successfully with both concentrate and forage feeds. NIR methods predict in vitro digestibility accurately and precisely, and can predict in vivo digestibility at least as well as conventional "wet chemistry" methods such as in vivo digestion or the pepsin-cellulase method, and much more rapidly. NIR technology has been applied to the routine monitoring (through analysis of feces samples) of the nutritional status of cattle and other grazing animals. This report reviews the use of near infrared reflectance(NIR) spectroscopy to monitor the nutritive value of animal feeds and the nutritional status of grazing animals.

Nutritive Value and Functional Properties of Protein Concentrate Fractionated from Chrysanthemum Flowers

  • Park, Nan-Young;Park, Yong-Jin;Kwon, Joong-Ho
    • Preventive Nutrition and Food Science
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    • v.3 no.2
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    • pp.175-179
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    • 1998
  • Some functional properties and nutritive value were determined for the protein concentrated fractionated from chrysanthemum flower in orer to renew interest in the flowers as food. Proximate components of chrysanthemum flower protein concentration (FPC) showed 61.2% protein, 2.0% fat and 35.2% carbhydrate on a dry basis. In amino acid composition of FPC, glutamic acid was the highest in the content, follwoed by aspartic acid, leucine and lysine. The ratio of essential/ total amino acids(E/T) was 0.42, showing a higher level of essential amino acids compared to the FAO reference protein. Digestibility of chrysanthemum FPC by pepsin and trypsin was lwoer than that of casein and was negatively correlative to both water and fat absorptions. Similar characteristics were determined between chrysanthemum FPC and milk casein in their emulsifying activity and emulsion stability. This results indicate that flowers or petals of chrysanthemum might be developed as a good source of protein.

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Effects of Oxidant, Reductant Treatment and Its Phosphorylation on Qualities and Functional Properties of Defatted Rice Bran Protein Isolates (산화.환원제처리 및 인산화가 분리탈지미강단백의 품질 및 기능적 성질에 미치는 영향)

  • Park, Wan-Kyu;Kim, Seung-Yeol;Lee, Ka-Sun
    • Korean Journal of Food Science and Technology
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    • v.20 no.1
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    • pp.106-111
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    • 1988
  • Comparative effects of oxidant, reductant treatment and its phosphorylation on qualities and functional properties of defatted rice bran protein isolates were investigated. Effects of oxidant and reductant treatment were that essential amino acid content of protein isolates was high and its color, pepsin digestibility were good. The phosphorylated defatted rice bran protein isolated was taken by incubating sodium trimeta phosphate in aqueous solution at pH 10.5 and $35^{\circ}C$ for 3 hours and its protein score was 55. Functional properties such as solubility, whipping activity and foam stability were much improved. But color, pepsin digestibility, bulk density and fat absorption were not affected by phosphorylation.

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Evaluation of Nutritional Characteristics of Different Sources of Food Residues in Autumn and Comparisons with NRC Nutrient Requirements for Swine (가을철 배출원별 남은 음식물의 사료 영양적 특성 및 돼지 영양소 요구량과의 비교 평가)

  • Kwak, W.S.;Kang, J.S.;Chung, J.D.
    • Journal of Animal Environmental Science
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    • v.8 no.2
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    • pp.87-98
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    • 2002
  • This study was conducted to determine nutritional values of different sources of food residues(FR) released in autumn and to compare them with nutrient requirements on NRC standard feeding system of swine. Hospital or cafeteria FR contained more cooked rice and side dishes residues and less vegetable residues and fruit peel, resulting in higher energy and lower fiber contents, compared to apartment complex FR, which had opposite patterns to these results. Chemical composition between hospital and cafeteria FR was almost similar. Salt(NaCl) content was more than 9 folds of NRC swine requirement, but much lower than the maximum tolerant level. Essenial and non-essential amino acids profile was similar among FR sources. Hospital or cafeteria FR protein had a similar pepsin digestibility to soybean meal protein. Apartment complex FR protein, however, had a much lower pepsin digestibility. When NRC nutrient requirements are considered, FR in swine diets could satisfy requirements of protein and all the essential amino acids, 75${\sim}$111% of digestible or metabolizable energy, and most of the major and minor minerals. All the FR contained extremely low levels of toxic heavy metals, indicating that they are completely safe from these toxic substances. It was concluded that hospital or cafeteria FR could be a nutritionally excellent and balanced feed source for swine.

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Studies on the Utilization of Keratins (경단백질(硬蛋白質)의 이용(利用)에 관(關)한 연구(硏究))

  • Suh, Hong-Kyl
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.5 no.1
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    • pp.75-80
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    • 1976
  • The development of protein utilization with keratin(cattle hair and human hair) have heen investigated. The include a) conditioning of hydrolysis of keratin, b) isolation of crude protein, c) pepsin HCl digestibility of isolated protein, d) the amino acid composition of isolated protein and amino acid concentrate. The results are obtained as follow ; 1. Human hair and cattle hair were softened and hydrolysed with hot dilute alkali(especially 1% NaOH) 2. Crude protein were isolated from hydrolysed keratin by means of different solubility and isoelectric point. 3. The protein isolated was of good quality on the point of color, protein content and pepsin digestibility. 4. The amino acid concentrate obtained by acids processing was of good quality on the point of color, taste and amino acid composition.

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Source of the Variation in Meat and Bone Meal Nutritional Quality

  • Hendriks, W.H.;Cottam, Y.H.;Morel, P.C.H.;Thomas, D.V.
    • Asian-Australasian Journal of Animal Sciences
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    • v.17 no.1
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    • pp.94-101
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    • 2004
  • The gross composition, gross amino acid content, apparent ileal amino acid digestibility and apparent ileal digestible amino acid content from 64 commercially produced meat and bone meals were statistically analysed. The samples were produced by 22 plants over a 2.5 year period with eight plants using batch dry rendering and 14 plants using low temperature rendering. A linear model with method and time of year (period) as fixed effects, plant within method as a random effect and sheep percent as a covariate was fitted to the composition data. The majority of the variation in the gross composition, amino acid digestibility and digestible amino acid content was explained by differences between plants using the same method. Neither rendering season nor origin of the raw materials contributed significantly to the observed variation in meat and bone meal protein quality. Rendering method (low temperature or batch rendering) had a significant effect on the variation observed in gross fat content, gross energy content, pepsin nitrogen digestibility, protein solubility and total lanthionine content. The digestibility of a number of amino acids and the apparent digestible content of arginine, cysteine, aspartic acid, proline and hydroxyproline were also significantly affected by rendering method. On average, batch dry and low temperature rendering systems produce meat and bone meals of similar nutritional quality. The variation between plant and within plant, however, is large, indicating that purchasing meat and bone meal from the same plant does not guarantee a consistent quality.

Effect of Lunasin Extracted from Millet (Panicum miliaceum) on the Activity of Histone Acetyltransferases, yGCN5 and p/CAF

  • Park, Jae-Ho;Jeong, Jin-Boo;Lee, Jeong-Rak;Lumen, Ben O. De;Jeong, Hyung-Jin
    • Korean Journal of Plant Resources
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    • v.22 no.3
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    • pp.203-208
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    • 2009
  • Lunasin is a unique 43-amino acid peptide which has shown a chemopreventive in mammalian cells and in a skin cancer mouse model. In search for new sources of lunasin and the role of cereals in cancer prevention, we report here the properties of lunasin purified from millet. Stability of millet lunasin was measured by in vitro digestibility assay using pepsin and pancreatin. Inhibition of HAT (histone acetyltransferase) and nuclear localization in mammalian cells were used to measure lunasin bioactivity as the cancer chemopreventive agent. Lunasin present in millet crude protein was stable to pepsin and pancreatin in in vitro digestion and inhibited the activities of HATs. When added exogenously, lunasin purified from millet internalized in the nuclei of mouse fibroblast cells. On the base of this result, we conclude that lunasin in millet is bioactive and consumption of millet may play an important role on cancer prevention in millet-consuming populations.

Effects of Substitution of Hydrolyzed Leather Meal for Fish Meal in the Broiler Diet (브로일러 사료에서의 피혁분의 어분대치 효과)

  • 강상렬;최진호
    • Korean Journal of Poultry Science
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    • v.10 no.1
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    • pp.30-34
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    • 1983
  • An experiment was carried out to evaluate the feeding value of hydrolyzed leather meal (HLM) replacing fish meal A total of 240 day-old female chicks of Maniker strain was divided into 20 groups of 12 birds ecah. Each groups was assigned to one of the five dietary treatments (0. 1. 2, 4 and 6% HLM). The feeding trial was conducted for six weeks. Results obtained are summarized as follows: 1. The HLM used in this experiment contained 74.41% crude protein. 17.0% crude ash and 2.0% chromium. Pepsin digestibility of the protein was measured to be 85%. Amino acid contents were also determined 2. The inclusion of HLM up to 4% in broiler rations did not depress the growth rate, feed consumption and feed efficiency of experimental birds. However, these performance parameters were significantly (p<0.05) depressed when fed 6% HLM. These results indicate that HLM in place of fish meal can be used up to 4% in tile broiler rations.

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Effects of ${\gamma}-Irradiation$ on Meat Proteins (감마선 조사가 육단백질에 미치는 영향)

  • Yook, Hong-Sun;Kim, Mee-Ree;Kim, Jung-Ok;Lim, Seong-Il;Byun, Myung-Woo
    • Korean Journal of Food Science and Technology
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    • v.30 no.2
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    • pp.407-412
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    • 1998
  • The proteins extracted from beef, pork and chicken meats were irradiated with up to 100 kGy at room temperature. The extracted proteins were evaluated on their in vitro digestibility by incubating successively with pepsin and pancreatin conjugate. Amino acid compositions and SDS-PAGE pattern were also analyzedin for these proteins. Gamma irradiation within the applied dose range (up to 100 kGy) produced negligible in in vitro digestibility and amino acid composition. Analysis of gamma-irradiated proteins by SDS-PAGE revealed radiolysis of ovalbumin to proteins or peptides with lower molecular weight. On the other hand, the proteins directly extracted from irradiated meats containing moisture were also evaluated for their in vitro digestibility, amino acid compositions and SDS-PAGE pattern. However, the results obtained from this experiment were similar to those of irradiated proteins after extraction from the meats.

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