• Title/Summary/Keyword: People Detection

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Detection of Genetically Modified Genes from Soybean Sprout Products (콩나물에서 발견된 유전자 변형 도입 유전자의 비의도적 혼입 조사)

  • 윤성철
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.49 no.3
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    • pp.227-231
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    • 2004
  • A total of 219 polymerase chain reaction tests of genetically modified (GM) DNA sequences in soybean seeds and soybean sprouts were conducted during 2000-2001. No CM gene was found in 96 tests of soybean seeds. However, either a functional CP4EPSPS gene or the 355 promoter gene was found three times in 2000 and eight times in 2001, in between 0.01 and 0.17% of soybean spout products, in 123 tests. Since the amount of GM genes was much less than the threshold limit of 3%, none of the 11 positive soybean-sprout samples needed to be libeled GM crops. Of these, seven sprout samples were from domestic seeds and four were from seeds imported from China. To find the contamination route, the raw materials, seed surface, floor of the storage room, area around the selection machine, surface of the packaging film and corn powder used in the package were tested. The 35S promoter gene was detected in only two samples of the corn powder (0.1%). Although we could not find the cause of the GM contamination, the sprout package film is one possibility. In total,8.9% of the soybean sprout tests were GM positive, but the amounts were much less than the threshold of 3%. This means that there are frequent false-positives and these would threaten the sprout industry if GMO were decided qualitatively. Food companies should make their safety data available to the public and make an effort to address people's concerns about GM food more openly. In addition, there is a need to establish a quantitative test for GM genes in sampled water and a sampling method for raw materials.

Measurement of Nanoaerosol Size Distributions and PAHs Detection After Cooking (삼겹살과 고등어 조리시 발생하는 나노입자의 시간과 거리에 따른 크기분포와 PAH 검출)

  • Hahn, Jung Suk;Woo, Chang Gyu;Noh, Seung Ryul;Bae, Yong Jun;Sung, Hyangki;Choi, Man Soo
    • Particle and aerosol research
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    • v.7 no.3
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    • pp.71-77
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    • 2011
  • Pork belly meat and mackerel are popular meals in Korea. Although a lot of people enjoy cooking these food, there have been some reports that generated organic particles during cooking could be thereas on of lung cancer of nonsmoking housewives. In addition, some experiments show that carcinogens may be included in meat and fishes which we eat usually. For this reason, particle size and concentration in formation during cooking are necessary to figure out the relationship between particles and the diseases. Thus, we identify number concentrations and size distributions of generated nano aerosol in cooking with respect to time, hood operation, and distance between cooking and measurement locations. The maximum concentrations of nano aerosol(diameter sizes are between 10 to 700nm)are decreased after the cooking from $8{\times}10^6{\sharp}/cm^3$ to zeroth order in pork belly meat cooking, and from $3.5{\times}{\times}10^6{\sharp}/cm^3$ to zeroth order in mackerel cooking respectively. When it comes to hood operation during cooking, the detected concentrations of generated aerosols are decreased as in taking flow rate of the hood increases. In cooking pork belly meat, the reduced amount of concentration is about $3{\times}10^6{\sharp}/cm^3$ compared to no hood operation, when hood in taking flow rate is $610m^3/hr$ In mackerel cooking, reduced concentration is $6{\times}10^5{\sharp}/cm^3$ in the same condition. Also, Naphthalene and Fluorene, which are known as polycyclic aromatic hydrocarbons (PAHs), are detected in the generated aerosols during cooking.

A Study of the Application of Earthquake Early Warning System for the Enhancements in Protective Action by Korea National Park (국립공원의 지진 대응 체계 개선을 위한 지진 조기경보 시스템의 적용에 관한 연구)

  • Yang, Eomzi;Ha, Seong Jun;Kim, Won Kyung;Yun, Tae Sup
    • KSCE Journal of Civil and Environmental Engineering Research
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    • v.38 no.3
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    • pp.439-448
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    • 2018
  • Conventional Earthquake Early Warning System (EEWS) detects the propagated P-wave from epicenter which should be achieved within 5 seconds to provide seconds to minutes of warning, allowing people to prepare for protective actions. EEWS in Korea is currently capable of providing a warning within 50 seconds after the primary P-wave detection, however, it is well-known that earthquake warning systems operating around Korean National Parks (KNP) have limited capability to fully monitor earthquake events. This study, therefore, presents a strategy to quantify the potential vulnerability to earthquake hazards by superimposing the distribution of Korea Integrated Seismic System (KISS) and the discretized map of KNP. Total 22 national parks are evaluated, and the results suggest that the improvement of the on-site systems should be necessitated for Gyoengju, Gyeryongsan, Songnisan, Gayasan, and Deogyusan national parks, whereas enhancement of regional systems is required for Bukhansan national park.

Development of a LC-MS/MS method for simultaneous analysis of 20 antihistamines in dietary supplements (LC-MS/MS를 이용한 건강기능식품 내 항히스타민 20종 동시분석법 개발)

  • Do, Jung-Ah;Kim, Jung Yeon;Choi, Ji Yeon;Lee, Ji Hyun;Kim, Hyung Joo;Noh, Eunyoung;Cho, So-Hyun;Yoon, Chang-Yong;Kim, Woo-Seong
    • Analytical Science and Technology
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    • v.28 no.2
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    • pp.86-97
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    • 2015
  • Recently, the consumption of dietary supplements has increased because of people’s greater interest in health. Unfortunately, the sales of dietary supplements containing unauthorized substances such as drugs have also increased. We developed a rapid, accurate method for the simultaneous determination of antihistamines using liquid chromatography tandem mass spectrometry with a multiple reaction monitoring mode. The limit of detection (LOD) and limit of quantification (LOQ) of the instrument used in this method were in the ranges 0.0003-0.3 and 0.0009-0.6 µg mL−1, respectively. The linearity of the method was > 0.99. The precision levels of the method were 0.1-9.8% (intra-day) and 0.3-9.6% (inter-day), and the levels of accuracy of the method were 82.7-115.0% (intra-day) and 84.3-113.0% (inter-day). The mean of recovery of the method was in the range of 83.9-117.9% and the RSD of the stability was less than 5.9%.

An Investigation on the Airborne Asbestos Concentrations using PCM and TEM in the Public Buildings in Seoul (PCM과 TEM을 이용한 서울지역 일부 공공 건축물의 실내공기 중 석면농도 조사)

  • Chung, Sook-Nye;Nam, Eun-Jung;Hwang, Soon-Yong;Oh, Seok-Ryul;Shin, Jin-Ho;Eom, Seok Won;Chae, Young-Zoo
    • Journal of Korean Society of Occupational and Environmental Hygiene
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    • v.21 no.3
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    • pp.139-145
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    • 2011
  • Objectives: This investigation is purposed to evaluate the airborne asbestos concentrations in the public buildings having asbestos containing materials(ACMs) in Seoul. Methods: The Seoul Metropolitan Government carried out an asbestos survey to the city-owned public buildings to identify the level of risk exposure, classified into low, moderate and high risk. To evaluate the airborne concentration of asbestos, 11 sampling sites in ten buildings based on the survey were selected. The air samples from the eleven sites were analyzed by Phase Contrast Microscopy(PCM) and Transmission Electron Microscopy (TEM), and compared the analytical results from the both. Results: 1. The airborne fiber concentrations by PCM were less than the detection limit($7f/mm^2$) in 9(82%) out of 11 sampling sites. The highest concentration was 0.0043 f/cc, but it was below the guideline value for indoor air quality(0.01 f/cc), proposed by the Ministry of Environment, Korea. 2. In two sampling sites, having moderate risk level, the chrysotile was identified and showed it's concentrations of 0.0102 s/cc and 0.0058 s/cc, less than $5{\mu}m$ lengths. 3. The ACMs identified in the two sampling sites were a packing material(65% of chrysotile) in mechanical area and a thermal system insulation(5% of chrysotile) in a boiler room. Having more possibility of asbestos emission in the mechanical area, it would be required to set up and carry out the asbestos management plan. Conclusions: Based on the result of this study, the airborne asbestos concentrations in the public buildings with ACMs were generally lower than the guideline value for indoor air quality. There are widespread concerns about the possible health risk resulting from the presence of airborne asbestos fibers in the public buildings. Most of the previous studies about airborne asbestos analysis in Korea were performed based on PCM method that asbestos and non-asbestos fibers are counted together. In the public and commercial buildings, having ACMs, it is suggested that the asbestos be analyzed by TEM method to identify asbestos due to concerns about asbestos exposure to workers and unspecified people.

Production of A Monoclonal Antibody (MAb) Against a Thermal Stable-Soluble Protein in Mackerel and Confirmation of the Properties for the MAb (고등어 어육 중 열안정성 단백질에 특이한 단클론성 항체 개발과 특성 확인)

  • Lee, Jeong-Eun;Kim, Jeong-Sook;Chung, Duck-Hwa;Shim, Won-Bo
    • Journal of Food Hygiene and Safety
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    • v.32 no.1
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    • pp.75-81
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    • 2017
  • For people who have a food allergy the only way to manage the allergy is to avoid the food allergen. The mackerel is one of the major food allergens, but no immunoassay for the rapid and simple detection of mackerel has been reported. The objectives of this study are to develop and characterize monoclonal antibodies (MAbs) specific to mackerel using thermal stable-soluble proteins (TSSP) as an immunogen and to characterize the MAbs by indirect enzyme-linked immunosorbent assay (iELISA). The mice immunized with mackerel TSSP and showing high titer were used for cell fusion and cloning. The characterization of MAbs produced from hybridoma cells obtained was confirmed by indirect ELISA and western blot. Four MAbs were confirmed to be specific to mackerel without cross-reaction to other marine products and livestock products in the both methods. The iELISA and western blot based on the MAbs can sensitively detect 1% mackerel protein in other marine products. These results support that immunochemical methods based on the MAb produced could be used as rapid means to detect low levels of mackerel and to identify mackerel adulterated in food.

Usefulness analysis of radial non-cartesian trajectory in the high-resolution MRA (고해상도 MRA 시 방사형 비직각좌표계 k-space 주사방식의 유용성 분석)

  • Lee, Ho-Beom;Choi, Kwan-Woo;Son, Soon-Yong
    • Journal of the Korea Academia-Industrial cooperation Society
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    • v.14 no.12
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    • pp.6284-6289
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    • 2013
  • With the application of k-space trajectory in a different manner and analyzing the influence of noise and its direction, this study was conducted to obtain high-quality images with minimal influence of noise during an MRI examination for cerebrovascular disease, which has a low signal for imaging. To evaluate influence of the noise of different k-space trajectories, a linear Cartesian coordination trajectory and non-Cartesian coordination trajectory were applied to 38 people who had received a high-resolution MRI examination for the early detection of cerebrovascular disease. As a result, the non-Cartesian coordination trajectory showed a 43.32% lower signal of lumens in the internal carotid artery than a linear Cartesian coordination trajectory, and the noise level was also 50.19% lower in a non-Cartesian coordination trajectory. This result shows that noise occurs less in a non-Cartesian coordination trajectory than a linear Cartesian coordination trajectory, and a non-Cartesian coordination trajectory is more effective in low-signal and low-resolution MRI examination. Therefore, when performing high-resolution MRI examination with a low-signal cerebrovascular system, the use of non-Cartesian coordination k-space trajectory will minimize the influence of noise and provide good images.

Recent Developments and Field Application of Foreign Waterworks Automatic Meter Reading (국외 상수도 원격검침시스템의 개발 동향 및 현장 적용 사례 고찰)

  • Joo, Jin Chul;Ahn, Hosang;Ahn, Chang Hyuk;Ko, Kyung-Rok;Oh, Hyun-Je
    • Journal of Korean Society of Environmental Engineers
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    • v.34 no.12
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    • pp.863-870
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    • 2012
  • The market trends of automatic meter reading associated with smart water meters were investigated. Also, recent developments and field applications of key technology for automatic meter reading associated with smart water meters were analyzed. Smart water meters have been manufactured mostly in United States and Europe and have been expanded their business to Asia. Integrated water management system combining with the additional functions such as real-time consumption metering, cost notification, water conservation, leak detection, water quality monitoring, and flow control have been operated in automatic meter reading. Both water quality and quantity data measured from smart water meters and sensors were transferred to data concentration units through neighborhood area network, and then were transferred to integrated server through wide area network. The data transfer methods were determined by comprehensively considering urban scale, density of smart water meters, power supply and network topologies. Common data collection methods such as fixed network to data concentation units, vehicles drive by, people walk by, and drone fly by have been applied. The automatic meter reading associated with smart water meters are spread throughout the world, and both water and energy savings result in saving the money and reducing the greenhouse gases emission.

Clinical Study of Tumor Angiogenesis and Perfusion Imaging Using Multi-slice Spiral Computed Tomography for Breast Cancer

  • Xu, Na;Lei, Zhen;Li, Xiao-Long;Zhang, Jun;Li, Chen;Feng, Guo-Quan;Li, Di-Nuo;Liu, Jing-Yi;Wei, Qiang;Bian, Ting-Ting;Zou, Tian-Yu
    • Asian Pacific Journal of Cancer Prevention
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    • v.14 no.1
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    • pp.429-433
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    • 2013
  • Objectives: To explore the correlation between multi-slice spiral CT (MSCT) perfusion parameters and the expression of vascular endothelial growth factor (VEGF) as well as matrix metalloproteinase-2 (MMP-2) in breast cancer. Methods: Forty five breast cancer patients and 16 patients with benign breast tumor, both confirmed by pathology examination, were enrolled. All underwent MSCT perfusion imaging to obtain perfusion maps and data for parameters including blood flow (BF), blood volume (BV) and permeability surface (PS). Cancer patients did not receive treatment prior to surgery. The expression of VEGF and MMP-2 were examined with both immunohistochemistry and Western blotting. Results: The levels of VEGF and MMP-2 by immunohistochemistry were significantly higher in the breast cancer group (P < 0.01) than the benign tumor group. Relative OD values from Western blotting were also higher in cancer cases (P < 0.05). Similarly, the mean MSCT perfusion parameters (BF, BV, PS) were significantly higher in the breast cancer group (P < 0.01), BF and BV positively correlating with VEGF expression (r = 0.878 and 0.809 respectively, P < 0.01); PS and VEGF and MMP-2 expression were also positively correlated (r= 0.860, 0.786 respectively, P < 0.01). Conclusion: There is a correlation between breast cancer MSCT perfusion parameters and VEGF andMMP-2 expression, which might be useful for detection of breast lesions, qualitative diagnosis of breast cancer, and evaluation of breast cancer treatment.

Determinants of Cervical Cancer Screening Uptake in Kurdish Women Living in Western Iran, 2014

  • Aminisani, N;Fattahpour, R;Abedi, L;Shamshirgaran, SM
    • Asian Pacific Journal of Cancer Prevention
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    • v.17 no.8
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    • pp.3763-3767
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    • 2016
  • Background: Cervical cancer is the second most common cancer among females across the world. It is a preventable cancer and early detection is very feasible. This study aimed to identify which women characteristics are potentially associated with and may have an important in uence on the uptake of cervical cancer screening in Kurdish women living in the west of Iran.. Materials and Methods: A cross sectional study was conducted in late 2014. A random sample of women aged 40 years and above without history of cervical cancer and identi ed as Kurdish background were selected and interviewed by two trained interviewers. Information about sociodemographic and reproductive factors, history of diseases, and cervical screening was collected using a questionnaire and women who had undergone a hysterectomy were excluded. Univariate analyses were used to describe the general characteristics of the study population. Multivariable logistic regression models with self-reported screening history were used to estimate odds ratios (ORs) with 95% con dence intervals (CI). Signi cance was considered at the 5% level. Results: A total of 561 women were included in this study (mean age $43.6{\pm}5.17$ years) participation in cervical screening at least once was about 32%. Cervical screening uptake percentage was signi cantly lower among people over 60 years of age (adjusted OR= 0.26, 95% CI: 0.11-0.64), and those who were illiterate (OR= 0.41 95% CI: 0.23-0.73) and post-menopausal (OR= 0.56, 95% CI: 0.35-0.91). Women with ${\leq}1$ child were less likely to report a Pap test (adjusted OR=0.43 95%CI: 0.13-1.37) Cervical screening uptake was higher among women with health insurance (OR= 2.31, 95% CI: 1.50-3.56). Conclusions: Cervical screening participation in this study was low compared to other studies in developed countries. The screening uptake was different based on age, education, parity, insurance coverage and menopausal status. It is recommended to target these groups of women in cervical screening program.