• Proceedings of the Plant Resources Society of Korea Conference
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• 2019.04a
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• pp.100-100
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• 2019

This study attempted to establish a High Performance Liquid Chromatography (HPLC) analysis method for the determination of (-)-epicatechin gallate as a part of the quality control for the development of functional cosmetic materials from Penthorum chinense Pursh extracts. HPLC was performed on a Unison US-C18 column ($4.6{\times}250mm$, $5{\mu}m$) with a gradient elution of 0.05% (v/v) trifluoroacetic acid (TFA) and methyl alcohol at a flow rate of 1.0 mL/min at $30^{\circ}C$. The analyte was detected at 280 nm. The HPLC method was performed in accordance with the International Conference on Harmonization (ICH) guideline (version 4, 2005) of analytical procedures with respect to specificity, precision, accuracy, and linearity. The limits of detection and quantitation were 0.11 and 0.33 mg/mL, respectively. Calibration curves showed good linearity (r2 > 0.9999), and the precision of analysis was satisfied (less than 0.6%). Recoveries of quantified compounds ranged from 99.51 to 101.92%. This result indicates that the established HPLC method is very useful for the determination of marker compound in P. chinense Pursh extracts.

• Journal of the Society of Cosmetic Scientists of Korea
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• v.45 no.1
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• pp.87-93
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• 2019

This study attempted to eatablish a High Performance Liquid Chromatography (HPLC) analysis method for the determination of (-)-epicatechin gallate as a part of the quality control for the development of functional cosmetic materials from Penthorum chinense Pursh. HPLC was performed on a Unison $US-C_{18}$ column ($4.6{\times}250mm$, $5{\mu}m$) with a gradient elution of 0.05% (v/v) trifluoroacetic acid (TFA) and methyl alcohol at a flow rate of 1.0 mL/min at $30^{\circ}C$. The analyte was detected at 280 nm. The HPLC method was performed in accordance with the International Conference on Harmonization (ICH) guideline (version 4, 2005) of analytical procedures with respect to specificity, precision, accuracy, and linearity. The limits of detection and quantitation were 0.11 and 0.33 mg/mL, respectively. Calibration curves showed good linearity ($r^2$ > 0.9999), and the precision of analysis was satisfied (less than 0.6%). Recoveries of quantified compounds ranged from 99.51 to 101.92%. This result indicates that the established HPLC method is very useful for the determination of marker compound in P. chinense Pursh extracts.

• Proceedings of the Plant Resources Society of Korea Conference
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• 2020.08a
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• pp.34-34
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• 2020

희귀식물(LC)인 낙지다리(Penthorum chinense Pursh.)는 '수택란', '차근채'로 불리며 약용으로 사용되어 왔으나, 최근 하천개수 및 습지매립 등으로 자생지가 파괴되어 개체수가 급감하고 있다. 따라서 본 연구는 낙지다리의 종 보존 및 활용을 위하여 종자의 최적발아조건을 확인하고자 수행하였다. 종자는 2017년 국립백두대간수목원에서 수집되어 연구에 사용되기 전까지 건조 상태(15℃, 15% RH)로 시드뱅크(-20℃, 40% RH)에 저장하고 있었다. 종자의 최적발아조건을 확인하기 위하여, 항온과 변온 66조건에서 발아율과 최종발아율의 50%가 발아하는데 소요되는 일 수(T₅₀)를 조사하였으며, 생리적 휴면 타파를 위해서 GA₃(20~1000 mg·L^-1) 처리 후 발아율과 T₅₀을 조사하였다. 그리고 GA₃ 처리에 따른 유묘 생장에 대한 GA₃의 영향을 확인하기 위하여 종자 치상 30일 후 유근·아 생장에 대한 영향을 조사하였다. 낙지다리 종자는 항온보다 변온에서 발아율 증가와 T₅₀ 감소를 보였다. 특히 낮/밤의 온도 차가 11~25℃일 때, 85%이상의 발아율을 보였고, 그 이상의 온도차이가 나면 발아율은 감소하는 경향을 보였다. T₅₀은 온도차가 증가할수록 감소하여, 발아가 균일하며 빨라지는 것을 확인 할 수 있었다. 또한 GA₃ 처리시 생리적 휴면 타파가 진행되어 발아율(79.8%~100%)과 발아속도가 증가(T₅₀ 감소)함을 확인하였으나, GA₃ 100mg·L^-1 이상의 농도에서는 무처리(21.2m)에 비하여 유근의 길이 생장을 0.11~6.88mm로 지연시켰다.

• Korean Journal of Environmental Biology
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• v.30 no.2
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• pp.107-120
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• 2012

This study was carried out to illiminate the diversity of vascular plants by making list of rheophyte and hydorphyte plants at Yugu stream. This study was conducted from Aug., 2009 to Sept. 2010 at 7 points. Based on the voucher specimens, vascular plants from investigated areas were 88 families 229 genera 290 species 3 subspecies 30 varieties 5 forms, totally 328 taxa. Korean endemic plants were 3 species such as Forsythia koreana (Rehder) Nakai (planted) and Paulownia coreana Uyeki (planted), and Weigela subsessilis (Nakai) L.H. Bailey. The rare and endangered plants were designated by Korea Forest Service were 4 taxa, such as Penthorum chinense Pursh, Acorus calamus L., Lilium callosum Siebold & Zucc. and Iris ensata var. spontanea (Makino) Nakai. Floristic special plants were totally 26 taxa, which were grade V of 2 taxa, grade IV of 2 taxa and grade III of 9 taxa. Naturalized plants were 27 taxa, that was 8.3% of whole discovered vascular plants in this area. Even naturalized plants has not influence on the vegetation of Yugu stream. Since village was composed along the stream and the roads and banks were linked, naturalized plants were flowed continuously into this region. So, strategy of the conservation for this region should be designed. Based on flora, 3 types of group were discriminated such as main stream type (4 points), branch stream type (2 points) and reservior type (1 point). Each group has difference in composition of plants.

• Korean Journal of Plant Resources
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• v.28 no.4
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• pp.475-486
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• 2015

This survey was conducted in order to investigate the vascular plants of Hak Reservoir Valley in the DMZ Area, Korea. The vascular plants were collected six times (from April 2014 to November 2014) and consisted a total of 238 taxa; 69 families, 172 genera, 206 species, 2 subspecies, 26 varieties and 4 forms. The observed rare plant species as designated by Korea Forest Service were 3 taxa including Carex capricornis Meinsh. ex Maxim., Acorus calamus L., Penthorum chinense Pursh. Endemic plant species were 2 taxa including Salix koriyanagi Kimura, Clematis brachyura Maxim. 17 taxa were listed as specific plant species based on phytogeographical in the investigated area. 25 taxa were recorded as naturalized plants, and their Naturalization Ratio and Urbanization Index were recorded as 10.46%, and 7.78%, respectively. Of the 238 taxa listed; 105 were pasturing plants, 92 edible plants, 76 medicinal plants, 24 ornamental plants, 6 stain plants, 4 timber plants, 2 fiber plants, 2 industrial plants and 55 unknown plants.

• Korean Journal of Environmental Biology
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• v.37 no.4
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• pp.690-706
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• 2019

Paddy fields play an important role in the biodiversity conservation of agroecosystems as man-made wetlands. This study focused on the floristic composition and characteristics of paddy fields in South Korea. The flora surveys were conducted in 33 areas in nine provinces at two times (June and August) in 2016. The results showed that the vascular plants of the paddy fields in South Korea included 389 taxa, composed of 51 families, 219 genera, 350 species, four subspecies, 30 varieties, and five forms. Among the 51 families, Gramineae was the most diverse in species (76 taxa), followed by Compositae (61 taxa), Leguminosae (32 taxa), Cyperaceae (27 taxa), Polygonaceae (15 taxa), and Scrophulariaceae (12 taxa). Based on the occurrence frequency of each species, Digitaria ciliaris (Retz.) Koel. (100%), and Echinochloa crus-galli (L.) P.Beauv. (100%) were the highest, followed by Acalypha australis L. (97.0%), Bidens frondosa L. (97.0%), Alopecurus aequalis Sobol. (97.0%), Rorippa palustris (Leyss.) Besser (93.9%), Ludwigia prostrata Roxb. (93.9%), Lemna perpusilla Torr. (93.9%), Mazus pumilus (Burm. f.) Steenis(90.9%), Rorippa palustris(Leyss.) Besser(87.9%), Eclipta prostrata(L.) L.(87.9%), Commelina communis L.(87.9%), Artemisia princeps Pamp.(84.8%), Erigeron annuus (L.) Pers.(84.8%), Cyperus microiria Steud.(84.8%), and Aneilema keisak(Hassk.) Hand.-Mazz. (81.8%). The biological type of paddy fields in South Korea was determined to be Th-R₅-D₄-t type. Rare plants were found in six taxa: Aristolochina contorta Bunge, Penthorum chinense Pursh, Sagittaria sagittifolia subsp. leucopetala (Miq.) Hartog, Ottelia alismoides (L.) Pers., Pseudoraphis ukishiba Ohwi, and Acorus calamus L. Eighty taxa contained naturalized plants composed of 15 families, 54 genera, 76 species, three varieties, and one form. The urbanization and naturalization indices were 24.8% and 20.6%, respectively.

• Korean Journal of Plant Resources
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• v.26 no.4
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• pp.463-490
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• 2013

This study was carried out to investigate the vascular plants of Namhan River. The period of survey was from March to November on 2012. Vascular plants based on voucher specimen were summarized as 430 taxa including 86 families, 250 genera, 383 species, 2 subspecies, 39 varieties and 6 forms. The rare plant species designated by Korea Forest Service were 13 taxa including Penthorum chinense Pursh, Polygonatum stenophyllum Maxim, and Sparganium erectum L., etc. And the endemic plant species were 8 taxa including Salix koriyanagi Kimura, Clematis trichotoma Nakai, and Carex gifuensis Franch., etc. Furthermore, 56 taxa were listed as specific plant species based on phytogeographical and based on the list of plants which have been approved for overseas delivery, 27 taxa were recorded in the investigated area. The naturalized plants were recorded as 58 taxa, and their Naturalization Ratio and Urbanization Index were recorded as 13.5%, and 18.1%, respectively. 430 taxa listed consists of 174 taxa(40.5%) of pasturing plant, 141 taxa(32.8%) of edible plants, 112 taxa(26.0%) of medicinal plants, 48 taxa(11.2%) of ornamental plants, 11 taxa(2.6%) of stain plants, 7 taxa(1.6%) of fiber plants, 4 taxa(0.9%) of timber plants, 2 taxa(0.5%) of industrial plants and 109 taxa(25.4%) of unknown plants.