• Journal of Microbiology
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• 제38권4호
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• pp.230-238
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• 2000

Class III chitin synthases in filamentous fungi are important for hyphal growth and differentiation of several filamentous fungi. A genomic clone containing the full gene encoding Chs4, a class III chitin synthase in Penicillium chrysogenum, was cloned by PCR screening and colony hybridization from the genomic library. Nucleotide sequence analysis and transcript mapping of chs4 revealed an open reading frame (ORF) that consisted of 5 exons and 4 introns and encoded a putative protein of 915 amino acids. Nucleotide sequence analysis of the 5'flanking region of the ORF revealed a potential TATA box and several binding sites for transcription activators. The putative transcription initiation site at -716 position was identified by primer extension and the expression of the chs4 during the vegetative growth was confirmed by Northern blot analysis. Amino acid sequence analysis of the Chs4 revealed at least 5 transmembrane helices and several sites for past-transnational modifications. Comparison of the amino acid sequence of Chs4 with those of other fungi showed a close relationship between P chrysogenum and genus Aspergillus.

• 미생물학회지
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• 제38권4호
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• pp.230-230
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• 2002

Class Ⅲ chitin synthases in filamentous fungi are important for hyphal growth and differentiation of several filamentous fungi. A genomic clone containing the full gene encoding Chs4, a class Ⅲ chitin synthase in Penicillium chrysogenum, was cloned by PCR screening and colony hybridization from the genomic library. Nucleotide sequence analysis and transcript mapping of chs4 revealed an open reading frame (ORF) that consisted of 5 exons and 4 introns and encoded a putative protein of 915 amino acids. Nucleotide sequence analysis of the 5′flanking region of the ORF revealed a potential TATA box and several binding sites for transcription activators. The putative transcription initiation site at -716 position was identified by primer extension and the expression of the chs4 during the vegetative growth was confirmed by Northern blot analysis. Amino acid sequence analysis of the Chs4 revealed at least 5 transmembrane helices and several sites for past-transnational modifications. Comparison of the amino acid sequence of Chs4 with those of other fungi showed a close relationship between P chrysogenum and genus Aspergillus.

• 미생물학회지
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• 제17권1호
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• pp.42-48
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• 1979

Pemicillum chrysogenum의 건전균(PC)과 바이러스감염균(PCV)을 사용하여 cellulase의 존재여부와 그 활성 정도를 알아보기 위해 밀기울에 배양하여 효소액으로 만들어 균의 생장에 따른 배양액의 최적 pH와 최적 배양일 및 Na-CMC 기질액을 이용하여 효소액의 활성에 미치는 최적 pH, 온도, 및 효소농도를 알아보고자 본 실험에 착수하여 다음과 같은 결과를 얻었기에 이에 보고하는 바이다. 1) PC외 PCV균의 생장에 따른 배양액의 최적 pH는 균주 접종전 배지와 작은 pH6.4로 나타났다. 2) 최적 배양일은 최적 pH를 나타낸 배양6일이 가장 높은 활성을 나타냈고, PCV는 PC의 50%의 활성이었다. 3) PC균 배양 효소액이 기질과 반응시 효소활성이 가장 높은 최적 pH는 pH5.0으로 나타났고, PCV 균 배양 효소액에서는 pH 6.0으로 나타났다. 4) PC와 PCV균 배양효소액 모두 기질과 반응시 효소 활성이 가장 높은 최적 온도는 $50^{\circ}C$로나타났다. 이상의 결과에서 cellulase의 활성도는 PC건전균이 PCV감입균보다 높은 활성을 나타낸 것을 알 수 있었다.