• 한국균학회지
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• 제32권1호
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• pp.16-22
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• 2004

Gibberellins(GAs)는 식물의 성장과 발전에 있어서 중요하게 작용한다고 알려져 있다. 따라서 본 연구에서는 농업과 원예분야에 매우 중요한 새로운 GAs 생성미생물을 탐색하고자 실험을 수행하였다. 생강나무(Lindera obtusiloba)와 산앵두나무(Vaccinium koreanum)의 뿌리에 존재하는 사상균을 분리하여 GAs 생산 활성을 측정하였으며, 비색법으로 GA 생산량을 분석하였을 때 이들 중에서 GAs 생산량이 가장 많은 것으로 확인된 두 균주에 대하여 여러 GAs 중에서 식물생장촉진 활성이 높다고 알려진 $GA_{1},\;GA_{3},\;GA_{4}$와 $GA_{7},\;GA_{53}$에 대하여 생산정도를 분석하고, 동정을 수행하였으며, Waito-c(난장이 볍씨)에서 Bioassay를 수행하였다. 생강나무에서는 6종의 균주, 산앵두나무에서는 4종류의 GAs 생산균을 분리하였고, 분리된 균중 GAs를 가장 많이 생산하는 균주들에 대하여 동정한 결과, 생강나무에서 분리한 C03 균주는 P. urticae KNUC03으로 동정되었으며, 산앵두나무에서 분리한 E03 균주는 P. griseofulvum KNUE03으로 동정되었다. 생합성된 GAs를 분석한 결과 P. urticae KNUC03 균주는 배양액 25 ml 중에 $GA_1\;7.08\;ng,\;GA_3\;30.80\;ng,\;GA_{4}\;1.27\;ng,\;GA_{7}\;0.88\;ng$ 및 $GA_{53}\;0.13\;ng$을 생산하였고 P. griseofulvum KNUE03은 $GA_1\;9.79\;ng,\;GA_3\;133.58\;ng,\;GA_4\;2.64\;ng,\;GA_7\;7.80\;ng$ 및 $GA_{53}\;0.73\;ng$을 생산하는 것이 확인되었다. 두 균주 중에서 P. griseofulvum KNUE03이 GAs를 더 많이 생산함을 알 수 있었다.

• 한국균학회소식:학술대회논문집
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• 한국균학회 2016년도 춘계학술대회 및 임시총회
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• pp.11-11
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• 2016

Penicillium species are commonly isolated from various outdoor and indoor environments, including marine environments such as sponges, algae and sand. Penicillium is especially important because numerous bioactive compounds have been isolated. Penicillium was the most common species in intertidal zone in Korea, however the diversity and ecological roles of Penicillium in intertidal zone are not clarified. We explored diversity and ecological roles of marine-derived Penicillium from tidal flat and sea sand in Korea. The diversity of marine-derived Penicillium from Korea was investigated using both culture-dependent and culture-independent approach by ${\beta}$-tubulin sequence. In addition, we evaluated optimal temperature, halo-tolerance, and enzyme activity of Penicillium strains, such as extracellular alginase, endoglucanase, ${\beta}$-glucosidase, and protease. For culture-dependent approach, a total of 182 strains of 62 Penicillium species were isolated, with 53 species being identified. The most common species was Penicillium oxalicum, followed by P. crustosum, P. brasilianum, P. koreense, and P. griseofulvum. Species richness and composition were not significantly different by season, substrates, and seaside. For culture-independent approach using Illumina sequencing, 73 OTUSs were detected. The most frequently observed species was P. antarcticum, followed by P. koreense, P. crustosum, and P. brevicompactum. Diversity of Penicillium was higher during winter season than during summer season and in western sea than in southern sea, respectively. Community structure was significantly different by season and sea side. 52 species were detected by both methods. Unique species were isolated from each of methods - 10 from culture methods and 21 from Illumina sequencing. Furthermore, salinity adaption of the Penicillium varied depending on species. Many Penicillium species showed endoglucanase, ${\beta}$-glucosidase, and protease activity. Some species including P. paneum and P. javanicum degraded the polycyclic aromatic hydrocarbons. Thus, our results demonstrate that intertidal zone in Korea harbors diverse Penicillium community and marine-derived Penicillium play important ecological roles as decomposers of organic material in marine environments.

• 대한약학회:학술대회논문집
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• 대한약학회 2001년도 Proceedings of International Convention of the Pharmaceutical Society of Korea
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• pp.276.1-276.1
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• 2001

• Food Science and Biotechnology
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• 제15권6호
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• pp.896-901
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• 2006

Patulin is a food mycotoxin which induces genotoxicity and acute intestinal disease in infants. Patulin mainly originates from fruit putrefactive moulds, especially in apples, which necessitates the maintenance of strong safety standards against patulin for fresh and processed apples. To investigate the patulin producing moulds in Korean apples, 16 morphological types of fungi were isolated from Korean apples and a patulin producing fungus was identified based on a sequence analysis of the region of internal transcribed spacers (ITS5-5.8S-ITS4 region, 505 base pair) and the 26 rRNA D1/D2 region (527 base pair). Morphological analyses were also performed. The isolated patulin producing fungus was found to a representative species of Penicillium crustosum. The maximal patulin production ability of the isolated fungus (P. crustosum) and the patulin producing standard strain (P. griseofulvum, ATCC 46037) in an SY broth medium were 0.32 and 2.46 mg/L, respectively.