• Korean Journal of Animal Reproduction
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• v.24 no.3
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• pp.255-262
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• 2000

The aim of this work was to study the effects of ascorbic acid (Asc) and/or ferrous sulfate (Fe$^{2+}$) and spernatozoa preincubation on the in vitro fertilization in porcine. Porcine follicular oocytes matured in culture were inseminated with frozen-thawed boar spermatozoa preincubated for 0, 1, 2, 3, 4 and 5h. The penetration rates (37~51%) were not significantly different between durations of spermatozoa preincubation in medium with 0.1 mM Asc. The addition of 1.0 mM Fe$^{2+}$ during spermatozoa preincubation were not significantly affecting the penetration rates (41~56%). When spermatozoa were preincubated with Asc and Fe$^{2+}$, the penetration rates had a tendency to increase with time of spermatozoa preincubation, and were significantly (P<0.05) higher in spermatozoa preincubated with that than without Asc and Fe$^{2+}$ for 5 h. On the other hand, when spermatozoa were preincubated in fertilization medium without Asc and/or Fe$^{2+}$, the penetration rates were significantly (P<0.05) higher in medium with Fe$^{2+}$ than with Asc or Asc and Fe$^{2+}$ for in vitro fertilization. The rate of polyspermy in penetrated oocytes in medium with Asc and Fe$^{2+}$ decreased with the period of spermatozoa preincubation. Despite different culture conditions for spermatozoa preincubation, no differences were observed in polyspermy rates in the presence of Asc and/or Fe$^{2+}$ These results indicate the advantage of preincubating spermatozoa with Asc and Fe$^{2+}$ and an addition of Fe$^{2+}$ during in vitro fertilization with spermatozoa preincubated maintain penetration potential without increased polyspermy rates on in vitro fertilization in porcine oocytes.on in porcine oocytes.

• Asian-Australasian Journal of Animal Sciences
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• v.23 no.11
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• pp.1421-1426
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• 2010

We investigated the effects of various concentrations of ${\beta}$-hydroxybutyrate (BHB, 0, 0.1, 1 and 10 mM), a ketone body, added to chemically-defined maturation medium with or without energy substrates (glucose, pyruvate and lactate) on nuclear maturation rates up to the metaphase stage of the second meiotic division (M-II stage). In addition, we also assessed the influence of BHB on glutathione content, sperm penetration rate and embryonic development up to the blastocyst stage of oocytes matured under the presence of these energy substrates. Nuclear maturation rates up to the M-II stage of oocytes matured with BHB in each concentration group did not show a significant increase compared with the control (0 mM) groups in both the presence and absence of energy substrates. Although glutathione contents were not significantly different in each BHB concentration group, the sperm penetration rate in the 1 mM BHB group was significantly higher (p<0.05) and the embryonic development rate of oocytes up to the blastocyst stage was significantly lower (p<0.05) than the respective values of the control groups. These results suggest that BHB added to a chemically-defined maturation medium may stimulate sperm penetration while inhibiting embryonic development of porcine oocytes.

• Journal of Korean Tunnelling and Underground Space Association
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• v.19 no.4
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• pp.611-634
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• 2017

In rapid urbanization, demand for utility tunnel increases more, and tunnel boring machine (TBM) has been used widely. Prediction of TBM penetration rate is important for proper estimation of construction period and cost. Although there are several methods, such as NTNU model and CSM model that require many input parameters, fundamental understanding on correlations between rock properties and TBM penetration rate is critical. In this study, we explored the brittleness indices of hard rocks according to various definitions, and the correlations between the brittleness indices and the TBM penentration rates.

• Advances in concrete construction
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• v.10 no.6
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• pp.463-478
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• 2020

Multiple mathematical modeling for prediction of slump, compressive strength and depth of water penetration at 28 days were performed using statistical analysis for the concrete containing waste limestone powder as partial replacement of sand obtained from experimental program reported in this research. To extract experimental data, 180 concrete cubic samples with 20 different mix designs were investigated. The twenty non-linear regression models were used to predict each of the concrete properties including slump, compressive strength and water depth penetration of concrete with waste limestone powder. Evaluation of the models using numerical methods showed that the majority of models give acceptable prediction with a high accuracy and trivial error rates. The 15-term regression models for predicting the slump, compressive strength and water depth were found to have the best agreement with the tested concrete specimens.

• Transactions of the Korean Society of Mechanical Engineers B
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• v.23 no.5
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• pp.577-586
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• 1999

Two problems with jet injection through the cylindrical film cooling hole are 1) penetration of jet into mainstream rather than covering the surface at high blowing rates and 2) nonuniformity of the film cooling effectiveness in the lateral direction. Compound angle injection is employed to reduce those two problems. Compound angle injection increases the film cooling effectiveness and spreads more widely. However, there is still lift off at high blowing rates. Shaped film cooling hole is a possible means to reduce those two problems. Film cooling with the shaped hole is investigated in this study experimentally. Film cooling hole used in present study is a shaped hole with conically enlarged exit and Inlet-to-exit area ratio is 2.55. Naphthalene sublimation method has been employed to study the local heat/mass transfer coefficient and film cooling effectiveness for compound injection angles and various blowing rates around the shaped film cooling hole. Enlarged hole exit area reduces the momentum of the jet at the hole exit and prevents the penetration of injected jet into the mainstream effectively. Hence, higher and more uniform film cooling effectiveness values are obtained even at relatively high blowing rates and the film cooling jet spreads more widely with the shaped film cooling hole. And the injected jet protects the surface effectively at low blowing rates and spreads more widely with the compound angle injections than the axial injection.

• Proceedings of the KSR Conference
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• 2011.10a
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• pp.261-267
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• 2011

The test fit has commonly used for the evaluation of the railbed condition, and indirect methods by using the compressional wave are also studied. the direct evaluation method by penetration test has not been studied. For the measurement of in-situ cone tip resistance of the railbed with minimizing the disturbance of the upper railbed. the cone penetrometer with the helical type outer rod(CPH) was developed. The outer rod, which has helical screw, is penetrated through the gravel layer and provides the reaction force for cone penetration testing. the cone tip resistances are measured by the mini cone penetrometer, where diameter is 15mm. For the developing the mini cone, strain gauge installation, circuit configuration, penetration rates and calibration process are considered. For the easy penetration of the screw rod in the field, the reaction force stepping plate and guide column are arranged. The screw rod are penetrated through the gravel layer. And the mini cone was pushed into the subgrade railbed at the penetration rate of 1mm/sec. The penetration test shows that the cone tip resistance increases along the depth. In addition, the subgrade condition is evaluated. This study demonstrates that the CPH may be effectively used for the evaluation of subgrade method any damage of the gravel layer.

• Clinical and Experimental Reproductive Medicine
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• v.16 no.1
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• pp.15-21
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• 1989

An in vitro fertilization assay employing zona-free hamster embryos was used to investigate human spermatozoal fertilitzing ability. Yanaghimarchi et al.(1976) first introduced this cross species fertilzation technique, with its application as a diagnostic tool for male infertility. Human spermatozoa were preincubated for 3 to 4 hrs in B W W medium at concentration of $4{\times}10^6$ sperm/ml prior to the addition to zona-free hamster embryos. After 3 hrs, human sperm was evaluated for fertilizing potential by the presence of swelling or decondencing sperm head in the cytoplasm. The results of penetration rates for sperm were as follow : 1. The average penetration rate of a 7 fertile donor group was $47.8{\pm}27.67%$(Range 14.3-98.0%) 2. The average penetration rate of 12 infertile patients with normal semen analysis was $21.7{\pm}26.9%$(Range 0-38.8%) 3. The average penetration rate of 10 infertile patients with semen abnormalities was $6.1{\pm}8.1%$(Range 0-25%)

• International Journal of Advanced Culture Technology
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• v.7 no.3
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• pp.10-24
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• 2019

This study aimed to investigate the effects and epidermis penetration system with polymer micelle of Red Pinus densiflora extract. In the antioxidant test, the total concentration of polyphenol compounds was determined to be $137.5163{\pm}7.70mg/g$ in ethanol extract, $133.956{\pm}1.57mg/g$ in hydrothermal extract. The DPPH radical scavenging effects were $95.29{\pm}0.15%$ in ethanol extract at 1,000 mg/L. Elastase inhibition rates were $100.00{\pm}2.85%$ in ethanol extract at 2,000 mg/L. The antimicrobial effect of the ethanol extraction was higher than that of hydrothermal extractions. In the epidermal permeability experiment, it was confirmed that the permeation of the polymer micelle containing the Red Pinus densiflora's ethanol extract and cell penetrating peptides was remarkable. Here, we confirmed that ethanol extract of Red Pinus densiflora displayed excellent the effects in antioxidant test and epidermis penetration system with polymer micelle. As a result, Red Pinus densiflora extract has potential to be used as a safe and natural cosmetic material in the future.

• Geomechanics and Engineering
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• v.18 no.3
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• pp.259-266
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• 2019

This paper presents a comparison between experimental measurements and numerical estimations of penetration length of a cement grout injected in discrete joints. In the experiment, a joint was generated by planar acryl plates with a certain separation distance (; aperture) and was designed in such a way to vary the separation distances. Since a cement grout was used, the grout viscosity can be varied by controlling water-cement (W/C) ratios. Throughout these experiments, the influence of joint aperture, cement grout viscosity, and injection rate on a penetration length in a discrete joint was investigated. During the experiments, we also measured the time-dependent variation of grout viscosity due to a hardening process. The time-dependent viscosity was included in our numerical simulations as a function of elapsed time to demonstrate its impact on the estimation of penetration length. In the numerical simulations, Bingham fluid model that has been known to be applicable to a viscous cement material, was employed. We showed that the estimations by the current numerical approach were well comparable to the experimental measurements only in limited conditions of lower injection rates and smaller joint apertures. The difference between two approaches resulted from the facts that material separation (; bleeding) of cement grout, which was noticeable in higher injection rate and there could be a significant surface friction between the grout and joint planes, which are not included in the numerical simulations. Our numerical simulation, meanwhile, could well demonstrate that penetration length can be significantly over-estimated without considering a time-dependency of viscosity in a cement grout.

• Proceedings of the Korean Radioactive Waste Society Conference
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• 2003.11a
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• pp.584-589
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• 2003

The hot cell facility which is designed to permit safe handling of source materials with radioactivity levels up to 1,385 TBq, is planned to be built. To meet this goal, the facility is designed to keep gamma and neutron radiation lower than the recommended dose-rate in normally occupied areas. The calculations performed with QAD-CGGP and MCNP-4C are used to evaluate the proposed engineering design concepts that would provide acceptable dose-rates during a normal operation in hot cell facility. The maximum effective gamma dose-rates on the surfaces of the facility at operation area and at service area calculated by QAD-CGGP are estimated to be $2.10{\times}10^{-3}$, $2.97{\times}10^{-2}$ and $1.01{\times}10^{-1}$ mSv/h, respectively. And those calculated by MCNP-4C are $1.60{\times}10^{-3}$, $2.99{\times}10^{-3}$ and $7.88{\times}10^{-2}$ mSv/h, respectively The dose-rates contributed by neutrons are one order of magnitude less than that of gamma sources, and penetration and toboggan will be partly reinforced by lead shield.