• Journal of Veterinary Clinics
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• v.24 no.2
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• pp.82-87
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• 2007

Five dogs with renal failure were referred to the Veterinary Medical Teaching Hospital at Kangwon National University. These dogs had the common history of consumption of Pedigree dry dog food produced in Thailand plant for over 1 month. The dogs showed anorexia, emaciation, vomiting, and polydipsia/polyuria. And in one severely affected dog, bloody diarrhea and hypothermia were seen. The remarkable clinicopathological signs were high value of BUN and creatinine. In some dogs, GGT, phosphorus and lipase were increased. However, no significant changes of complete blood count were found. In urinalysis, hematuria, low specific gravity urine, proteinuria, and calcium oxalate-like crystals were observed. Two severely affected dogs were died. The remained dogs were recovered gradually after change of dog food and supportive therapy. Pathological findings were seen typically in kidneys. Renal atrophy, congestion of the glomerular capillary, and diffuse degeneration, necrosis, dystrophic calcification and regeneration in the tubular epithelium were seen. Yellowish brown fluorolucent laminated materials or particles were quite often found in the lumina of the necrotizing renal tubules of cortex and medulla. Proliferation of fibrous tissue in the interstitium was also seen. By the mycotoxin analysis of the Pedigree dry dog food, ochratoxin A (OTA) and citrinin were detected as much as the concentration of 372.8 ppb and 8.3 ppb, respectively. The final diagnosis of renal failure caused by OTA and citrinin toxicosis was made on the basis of history takings, clinical signs, clinicopathological and pathological findings, and analysis of mycotoxins.

• Korean Journal of Agricultural Science
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• v.41 no.1
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• pp.41-46
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• 2014

The pedigree data of 86,639 heads from six major swine breeding farms were investigated to check levels of inbreeding and effective population size of breeding stocks. The average rate of inbreeding was 1.04%, 0.87%, 3.17%, 1.05%, 3.23% and 3.33% for farms A, B, C, D, E and F, respectively. The average inbreeding rate was highest for F farm and lowest for B farm. In farms D and E, there were quite large numbers of immigrant animals per generation compared to other farms. The effective population sizes calculated from the average rate of inbreeding were distributed between 83.0 and 814.8. Specific values were 282.3, 225.5, 83.0, 814.8, 302.9 and 175.7 for farms A, B, C, D, E and F, respectively. The results showed that there was no cause for concern over the current inbreeding level of major swine breeding farm populations and the inbreeding level was within an acceptable range. In addition, internal sharing rather than importing pig is necessary to strengthen seed sovereignty.

• Journal of Life Science
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• v.15 no.1 s.68
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• pp.45-48
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• 2005

The aim of this study was to construct a correct pedigree of miniature horses (MH). The sex of MH was detected by PCR amplification of the sex determining region of the Y chromosome gene (SRY) prior to parentage testing. Ten random MH samples for parentage testing were genotyped by using 16 micro satellite markers. Since the SRY band (430 bp) was detected in horses No.1, 2, 6, 7, 8, 9, 10, these are male. However, the DNA segment was not identified in horses No.3, 4, and 5, which therefore are female. After genotyping, parentage testing was performed according to Mendelian fashion and International Society for Animal Genetics (ISAG) guideline. Of the 10 MH, 3 were qualified by the compatibility of 16 markers according to Mendelian fashion in the present DNA typing for parentage verification. These results can provide basic information for developing parentage verification and an individual identification system in MH.

• Horticultural Science & Technology
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• v.16 no.1
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• pp.18-20
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• 1998

The present research was conducted to study genetic relationship and cultivar identification in sweet potato (lpomoea batatas) using RAPD method. Thirteen cultivars of sweet potato in Korea were classified by UPGMA clustering method into three groups as follows; group I was corresponded to 'Choongsung100'; group II, 'Eunmi', 'Saengmi', 'Suwon147' and 'Yulmi'; group III, 'Hongmi', 'Jinmi', 'Kwandong95', 'Seonmi', 'Wonmi', 'Shinyulmi', 'Jeungmi', and 'Poongmi'. Identification using RAPD was generally consistent with breeding pedigree of those parents. However, inconsistent results may be caused by clonal variation. The results presented in this study suggest that RAPDs in sweetpotato are likely to be useful for cultivar identification and various procedures in breeding. The use of various DNA marker system assists selection programs for economically important trait, and may facilitate selection in earlier growing stage. This systems may enhance the prospects for improving sweet potato cultivar by accurate marking desirable traits at DNA level.

• Journal of agriculture & life science
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• v.50 no.2
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• pp.117-124
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• 2016

This study was estimated the effect of environmental factors and each of them on auction price of Hanwoo calves. The auction prices of Hanwoo calves(19,592head) registered pedigree were sampled in Gyeongsangnam-do from 2014 to 2015. The environmental factors were highly(p<0.01) affect to the auction price of Hanwoo calves. With the analysis of contribute factor used the semi-partial correlation, the value of regression equation(Adj R-Square) was 0.701. The semi-partial correlation values of sex, auction body weight, auction age, auction year, auction season, and auction area were 0.11563, 0.20013, 0.02823, 0.10727, 0.00330 and 0.02963, respectively. And the highest contribution factor was body weight, and then, followed sex, auction year, auction area and auction age. In conclusion, it could be suggested that Hanwoo calf farmer should improve calf body weight to increase auction price by not only breeding, but also optimal management. Additionally, it is expecting in the future that competitiveness and income of farmers will be affected deeply by the holding and producing of predominant genes which is coincidence with breeding principles. However, further study should be conducted for the better understand caused by the limit of previous studies which was estimated the correlations of auction price and each factors.

• Journal of Animal Science and Technology
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• v.54 no.5
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• pp.337-340
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• 2012

The genetic connectedness between herds is an essential requirement to make robust across-herd estimation of the breeding values of the animals. In this study, genetic connectedness between herds was evaluated by a connectedness rating method. A total of 24,971 records of days to 90 kg (D90KG) of the pigs on performance testing programs collected from six herds (labeled from 'A' to 'F') of Duroc breed along with pedigree information comprising 456,697 families were used. Results showed that a total of eight boars were used for semen exchange programs among participant farms. Herds 'A' through 'E' were found strongly connected among them. But 'F' herd was genetically connected strongly only with 'A' herd. The highest average connectedness rating was 91.7% between 'A' herd and 'C' herd. The lowest average connectedness rating was 65.1% between 'D' and 'F'. The concept of a single genetic group comprising six Duroc herds studied is meaningful due to high connectedness rates among them. Therefore, with this high genetic ties between participant Duroc farms, the more accurate genetic evaluation would be possible.

• Journal of Animal Science and Technology
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• v.51 no.5
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• pp.345-352
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• 2009

This study utilized 332,625 records of linear type scores consisting for 15 primary traits, 22,175 final score and 84,612 pedigree information of 22,175 Holstein cows from 1993 to 2007 in Korea to estimate genetic parameters for 16 type traits. Genetic and error (co)variances between two traits selected from 16 traits were estimated using bi-trait pairwise analyses with DFREML package. The estimated heritabilities for stature (ST), strength (STR), body depth (BD), dairy form (DF), rump angle (RA), thurl width (TW), rear legs side view (RLSV), foot angle (FA), fore udder attachment (FUA), rear udder height (RUH), rear udder width (RUW), udder cleft (UC), udder depth (UD), front teat placement (FTP), front teat length (FTL) and final score (FS) were 0.31, 0.21, 0.25, 0.10, 0.29, 0.19, 0.09, 0.06, 0.12, 0.13, 0.12, 0.08, 0.26, 0.20, 0.28 and 0.15, respectively. ST had the highest positive genetic correlation with BD (0.90), while RLSV had the highest negative genetic correlation with FA (-0.56). RA had negative genetic correlation with most udder traits (-0.17~-0.02). Especially, RUW had the higher positive genetic correlation with STR (0.60), BD (0.62), and TW (0.49), however, UD had the higher negative genetic correlation with STR (-0.40) and BD (-0.40). FTL had negative genetic correlation with FUA, RUH, RUW, UC and UD. FS had positive genetic correlation with UC, UD and FTP (0.12, 0.18 and 0.20). However, additional research is needed on the use of these parameters in the genetic evaluation because estimated genetic and error variance-covariance matrices were not positive definite.

• Journal of The Korean Society of Grassland and Forage Science
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• v.33 no.2
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• pp.81-86
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• 2013

'Mogwoo', a new high yield and whole crop silage rice (Oryza sativa L.) cultivar, was developed by the rice breeding team of the National Institute of Crop Science, RDA, Suwon, Korea, from 1999 to 2009, and was released in 2010. It was derived in 1999 from a cross between Dasanbyeo, having a high yield, and Suweon431/IR71190-45-2-1. A promising line, SR25848-C99-1-2-1, selected by the pedigree breeding method, was designated the name of 'Suweon 519' in 2007. This cultivar has about 155 days of growth period from seeding to heading, and is tolerance to lodging, with erect pubescent leaves as well as a long and thick culm. This cultivar has the same number of tillers per hill and higher spikelet numbers per panicle compared to Nokyang. 'Mogwoo' has longer leaves compared with other Tongil-type varieties. This new variety is resistant to grain shattering, leaf blast, bacterial leaf blight, and small brown planthopper. The biomass yield of 'Mogwoo' was 1,956 kg/10a in a regional test over three years. The result shows that 'Mogwoo' is adaptable to central and south-east plain areas of Korea.

• Journal of Animal Science and Technology
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• v.55 no.3
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• pp.165-171
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• 2013

Genomic relationship matrix (GRM) was constructed using whole genome SNP markers of swine and genomic breeding value was estimated by substitution of the numerator relationship matrix (NRM) based on pedigree information to GRM. Genotypes of 40,706 SNP markers from 448 pure Landrace pigs were used in this study and five kinds of GRM construction methods, G05, GMF, GOF, $GOF^*$ and GN, were compared with each other and with NRM. Coefficients of GOF considering each of observed allele frequencies showed the lowest deviation with coefficients of NRM and as coefficients of GMF considering the average minor allele frequency showed huge deviation from coefficients of NRM, movement of mean was expected by methods of allele frequency consideration. All GRM construction methods, except for $GOF^*$, showed normally distributed Mendelian sampling. As the result of breeding value (BV) estimation for days to 90 kg (D90KG) and average back-fat thickness (ABF) using NRM and GRM, correlation between BV of NRM and GRM was the highest by GOF and as genetic variance was overestimated by $GOF^*$, it was confirmed that scale of GRM is closely related with estimation of genetic variance. With the same amount of phenotype information, accuracy of BV based on genomic information was higher than BV based on pedigree information and these symptoms were more obvious for ABF then D90KG. Genetic evaluation of animal using relationship matrix by genomic information could be useful when there is lack of phenotype or relationship and prediction of BV for young animals without phenotype.

• Horticultural Science & Technology
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• v.34 no.3
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• pp.488-494
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• 2016

To develop appropriate potato clones as functional food materials, we collected 35 potato breeding clones to analyze the contents of the total polyphenol, anthocyanin, and vitamin C with the 'Superior', 'Dasom valley', and 'Gogu valley' cultivars as controls. Based on our analysis great differences were observed in different potato clones. KPG16 had the highest content of total polyphenol at $105.08mg{\cdot}100g^{-1}\;FW$; KPG13 had the highest content of anthocyanin at ${\cdot}4.78mg100g^{-1}\;FW$; KPG20 had the highest content of vitamin C at $22.16mg{\cdot}100g^{-1}\;FW$. Some clones had higher contents of the total polyphenol but lower levels of anthocyanin. Ultimately, potato clones showing relatively high indexes for all three compounds, could be considered as good functional food material. By equilibrium analysis of the contents of total polyphenol, anthocyanin, and vitamin C, KPG5 showed relatively higher contents, with values of 103.95, 3.15, $12.12mg{\cdot}100g^{-1}\;FW$, respectively. Therefore, KPG5 was considered to be the best potato breeding clone in view of a functional potato breeding system.