• Nutrition Research and Practice
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• 제8권1호
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• pp.33-39
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• 2014

Obesity occurs when a person's calorie intake exceeds the amount of energy burns, which may lead to pathologic growth of adipocytes and the accumulation of fat in the tissues. In this study, the effect and mechanism of pear pomace extracts on 3T3-L1 adipocyte differentiation and apoptosis of mature adipocytes were investigated. The effects of pear pomace extract on cell viability and the anti-adipogenic and proapoptotic effects were investigated via MTT assay, Oil red O staining, western blot analysis and apoptosis assay. 3T3-L1 preadipocytes were stimulated with DMEM containing 10% FBS, 0.5 mM 3-isobutyl-1-methylxanthine (IBMX), $5{\mu}g/ml$ insulin and $1{\mu}M$ dexamethasone for differentiation to adipocytes. 3T3-L1 cells were cultured with PBS or water extract of pear pomace. Water extract of pear pomace effectively inhibited lipid accumulations and expressions of PPAR-${\gamma}$ and $C/EBP{\alpha}$ in 3T3-L1 cells. It also increased expression of p-AMPK and decreased the expression of SREBP-1c and FAS in 3T3-L1 cells. The induction of apoptosis was observed in 3T3-L1 cells treated with pear pomace. These results indicate that pear pomace water extract inhibits adipogenesis and induces apoptosis of adipocytes and thus can be used as a potential therapeutic substance as part of prevention or treatment strategy for obesity.

• 한국식품과학회지
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• 제31권4호
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• pp.971-976
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• 1999

WAIP에 존재하는 총 galacturonic acid의 함량은 34.6%로 측정되었으며, 산처리를 통한 펙틴 추출수율은 약6.2%로 나타났다. EPG는 불용성의 프로토펙틴을 수용화시켜 펙틴을 생산하는 효소로 최적조건시 높은 수율을 나타낼 수 있기 때문에 EPG를 이용하여 펙틴추출 조건을 온도, 시간, pH, 효소 첨가량 등을 조사하였다. 먼저 최적조건을 조사한 결과 $60^{\circ}C$, 36시간, pH 7.8로 그 추출률은 23.2%로 측정되었다. 기질과 효소 반응 최적비를 측정한 결과 10:1(w/w)에서 23.4%로 가장 높은 추출 수율을 나타내었다. 펙틴의 순도를 측정한 결과 산 추출 펙틴은 71.7%이었고, 효소를 이용하여 추출한 펙틴은 34.7%로 나타났다. 또한 methoxyl 함량을 측정한 결과 산 추출 펙틴은 5.0%, 효소 추출 펙틴은 0.7%로 저 methoxyl 펙틴으로 나타났다. 한편, 추출된 펙틴의 평균 분자량은 효소 추출 펙틴의 경우 $2.5{\times}10^{3}$이었으며, 산 추출 펙틴의 경우 $8.4{\times}10^{3}$으로 나타나 분자량이 적은 펙틴이 추출되었다.