• Title/Summary/Keyword: Peanut

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Effect of Peanut Seed Orientation on Germination, Seedling Biomass, and Morphology in an Oak Tree Sawdust Cultivation System

  • Ahn, Junsik;Song, Ilchan;Kim, Dongjae;Lee, Joon Chul;Moon, Sungkwon;Myoung, Sooncheol;Ko, Kisung
    • Horticultural Science & Technology
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    • v.35 no.4
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    • pp.402-409
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    • 2017
  • We performed seed germination tests to investigate the effects of seed sowing orientation on germination viability on peanut (Arachis hypogaea L.) sprouts. Specifically, we assessed the influence of seed sowing orientation on germination rate, seedling weight, and seedling length, as well as the seedling vigor index. The seeds were sown in oak tree sawdust at 3.0 cm depth. Four seed orientations were tested: vertical with the hypocotyl end down, vertical with the hypocotyl end up, horizontal with the hypocotyl end down, and horizontal with the hypocotyl end up. The mean seed germination percentages of the four seed orientations were significantly different (p < 0.01) and ranged from 25 to 91.7%. The vertical orientation with hypocotyl-end-down and hypocotyl-end-up orientations showed the highest (91.7%) and lowest (25%) germination rates, respectively. The vertical orientation with the hypocotyl end down produced the heaviest (4.9 g) seedlings and the longest hypocotyls (4.65 cm). This orientation also produced the longest true leaf + epycotyl (2.15 cm) and had the highest seedling vigor index (197.1). The seedlings had a straight growth pattern, whereas seedlings from seeds sown with the hypocotyl up had an awkward plumular hook shape. Taken together, to produce peanut sprouts, we recommend placing the seeds vertically with the hypocotyl end down because this orientation leads to a high germination rate, high biomass production, and high overall seedling quality.

Effects of Organic Materials Application on Growth of Peanut Plant (유기물(有機物) 시용(施用)이 땅콩 생육(生育)에 미치는 영향(影響))

  • Hwang, Nam-Yul;Chae, Jae-Seok;So, Jae-Don
    • Korean Journal of Soil Science and Fertilizer
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    • v.18 no.2
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    • pp.215-220
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    • 1985
  • This experiment was conducted to find out the effects of organic materials application on the growth of peanut, yield and chemical properties in mature field and newly reclaimed upland soil. The result can be summarized as follow. 1. Application of rice straw and rice hull increased the yield of peanut in mature field and compost plot was more yield than any other plot in newly reclaimed upland soil. 2. Rice straw and rice hull plot was heavier the weight of needles per plant than compost plot in newly reclaimed soil. 3. There was high correlation between soil O.M. at flowering stage and yield in newly reclaimed upland soil but was not significant in mature field. 4. Contents of soil O.M. did not change in mature field however this was increased tendency in newly reclaimed upland soil.

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Peanut sprout ethanol extract inhibits the adipocyte proliferation, differentiation, and matrix metalloproteinases activities in mouse fibroblast 3T3-L1 preadipocytes

  • Kim, Woo Kyoung;Kang, Nam E;Kim, Myung Hwan;Ha, Ae Wha
    • Nutrition Research and Practice
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    • v.7 no.3
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    • pp.160-165
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    • 2013
  • 3T3-L1 preadipocyte were differentiated to adipocytes, and then treated with 0, 10, 20, and $40{\mu}g/mL$ of peanut sprout ethanol extract (PSEE). The main component of PSEE is resveratrol which contained 5.55 mg/mL of resveratrol. The MTT assay, Oil-Red O staining, glycerol-3-phosphate dehydrogenase (GPDH) activity, and the triglyceride concentration were determined in 3T3-L1 cells. MMP-2 and MMP-9 activities as well as mRNA expressions of C/EBP ${\beta}$ and C/EBP ${\alpha}$ were also investigated. As the concentration of PSEE in adipocytes increased, the cell proliferation was decreased in a dose-dependent manner from 4 days of incubation (P < 0.05). The GDPH activity (P < 0.05) and the triglyceride concentration (P < 0.05) were decreased as the PSEE treatment concentration increased. The mRNA expression of C/EBP${\beta}$ in 3T3-L1 cells was significantly low in groups of PSEE-treated, compared with control group (P < 0.05). The MMP-9 (P < 0.05) and MMP-2 (P < 0.05) activities were decreased in a dose-dependent manner as the PSEE concentration increased from $20{\mu}g/mL$. In conclusion, it was found that PSEE has an effect on restricting proliferation and differentiation of adipocytes.

Isolation and Biological Activity of $Resveratrol-3-O-{\beta}-D-Glucoside$ in Transgenic Rehmannia glutinosa L. Transformed by Peanut Resveratrol Synthase Gene (RS3)

  • Lim, Jung-Dae;Yang, Deok-Chun;Yun, Song-Joong;Chung, Ill-Min;Sung, Eun-Soo;Kim, Myong-Jo;Heo, Kweon;Yu, Chang-Yeon
    • Korean Journal of Medicinal Crop Science
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    • v.12 no.5
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    • pp.406-414
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    • 2004
  • Resveratrol, which is both a phytoalexin with antifungal activity and a phytochemical associated with reduced cancer risk and reduced cardiovascular disease, is synthesized in a limited number of plant species including peanut. Resveratrol synthesis is catalyzed by the enzyme stilbene synthase including resveratrol synthase (RS). Resveratrol synthase gene (RS3) obtained from peanut, Arachis hypogaea, Fabaceae has been transferred into chinese foxglove, Rehmannia glutinosa by using Agrobacterium mediated transformation. RS t-DNA introduced to chinese foxglove (R. glutinosa L) by transformation and its reaction product, $resveratrol-3-O-{\beta}-D-glucoside$ was isolated and characterized using HPLC. Also its biological effects was tested in inhibition of the lipid peroxidation of mouse LDL by glycosylated stilbenes derivatives obtained from transgenic plants. $Resveratrol-3-O-{\beta}-D-glucoside$ isolated from transgenic R. glutinosa L. showed antimicrobial activity of the growth inhibition zone against Escherichia coli and Salmonella typhimurium. Therefore, this compound can be contributed to be useful as a phytoalexin for plant health as well as a phytochemical for human health.

Characterization of Peanut stunt virus Isolated from Black Locust Tree (Robinia pseudo-acacia L.)

  • Bang, Ju-Hee;Choi, Jang-Kyung;Lee, Sang-Yong
    • The Plant Pathology Journal
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    • v.22 no.2
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    • pp.125-130
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    • 2006
  • An isolate of Peanut stunt virus (PSV) isolated from black locust tree (Robinia pseudo-acacia L.) showing severe mosaic and malformation symptoms, was designated as PSV-Rp. PSV-Rp was characterized by the tests of host range, physical properties, RNA and coat protein composition and RT-PCR analysis. Nucleotide sequences of the cucumoviruses CP genes were also used for identification and differentiation of PSV-Rp. Six plant species were used in the host range test of PSV-Rp. PSV-Rp could be differentiated from each Cucumovirus strain used as a control by symptoms of the plants. The physical properties of PSV-Rp virus were TIP $65^{\circ}C$, DEP $10^{-3}$, and LIP $2{\sim}3$ days. In dsRNA analysis, PSV-Rp consisted of four dsRNAs, but satellite RNA was not detected. Analysis of the coat proteins by SDS-PAGE showed one major protein band of about 31 kDa. RT-PCR using a part of Cucumovirus RNA3 specific primer amplified ${\sim}950bp$ DNA fragments from the crude sap of virus-infected black locust leaves. RFLP analysis of the RT-PCR product could differential PSV-RP from CMV The nucleotide sequence identity between the PSV-Rp CP and the TAV-P CP genes and the PS-V-RP CP and CMV-Y CP genes were 61.6% and 40.5%, respectively. On the other hand, the nucleotide sequence identity of the PSV-Rp CP gene was $70.9%{\sim}73.4%$ in comparison with those of PSV subgroup I (PSV-ER and PSV-J) and 67.3% with that of PSV subgroup II(PSV-W). Especially, the nucleotide sequence identity of PSV-Rp CP gene and that of PSV-Mi that was proposed recently as the type member of a novel PSV subgroup III was 92.4%.

Study on the Cause of Injury by Continuous Cropping of Peanut (땅콩의 연작(連作) 장해(障害) 원인(原因) 조사(調査))

  • Ryu, Jeong;Na, Jong-Seong;Hwang, Nam-Yul
    • Korean Journal of Soil Science and Fertilizer
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    • v.25 no.3
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    • pp.270-274
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    • 1992
  • This study was observed to find out the relationship of soil microorgaism physico-chemical properties and yields on the continuous cultivation of peanuts soil in the difference 20 farmes field located in Gochang, Chonbuk area. The results obtained was summarized as follows : 1. Soil hardness and volume of soild and liquid of peanut continuous cultivation soil were higher than those of one year cultuvation. 2. The exceeding optimum level of phosphorus and potassium appeared factors affecting and reduction of peanut continuous cultivation soil. 3. Number of microorgaisms in continuous cultivation soil was high but the ratio of bacteria/fungi (B/F) and actinomycetes/fungi (A/F) ratio were low. 4. The number of soil nematodes in continuous cultivation soil were higher than that of alternative cultivation soil.

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