• BMB Reports
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• 제45권5호
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• pp.287-292
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• 2012

FGF-2 is involved in cell survival, proliferation, apoptosis, and angiogenesis in a wide variety of cells. FRGRs, PI3K and MAP kinases are well known mediators of FGF signaling. Despite its known roles during many developmental processes, including osteogenesis, there are few known targets of FGF-2. In the present study, we identified Bcl2-A1 and Bcl-xL as two prominent targets involved in promoting cell survival. Pretreatment of ATDC5 cells with FGF-2 increased cell survival, while siRNAs specific for Bcl2-A1 and Bcl-xL compromised the anti-apoptotic effect of FGF-2, sensitized the cells to apoptosis triggered by TNF-${\alpha}$. Chemical inhibition of FGFR, NFkB, and PI3K activity by PD173074, pyrrolidine dithiocarbamate, and LY294002 respectively abrogated the FGF-2-mediated induction of Bcl2-A1 and Bcl-xL expression. Taken together, our data demonstrate that a subset of Bcl2 family proteins are the targets of FGF-2 signaling that promotes the survival of ATDC5 cells.

• 대한불안의학회지
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• 제5권2호
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• pp.125-132
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• 2009

Objective : This study examined psychiatric characteristics of wounded persons of Daegu subway fire accident occurring in February 2003 after six years. Methods : 103 wounded persons completed psychometric measures at 2 months and 6 years after the accident. Changes of psychiatric symptoms were compared and factors affecting post-traumatic stress disorder (PTSD) seriousness were examined. Results : In Minnesota Multiphasic Personality Inventory, the scores of Psychopathic Deviate (Pd), Masculinity/ Femininity (Mf), Social Introversion (Si) scales were significantly changed over time. The scores of Symptoms Checklist-90-Revision subscales were mostly decreased over time, however, paranoid ideation (PAR) subsclae scores were not significantly changed over time. In all of the State-Trait Anxiety Inventory (STAI)-State, Beck Depression Inventory, Impact of Event Scale, and PTSD Checklist-Civilian, mean scores at 6 years after the accident were significantly lower than those at 2 months after the accident. However, STAI-Trait score was not significantly changed over time. Total seriousness of PTSD was found to be affected by quality of sleep. Conclusions : Psychiatric symptoms of subjects were largely improved over time, but distrust and doubt of others were continued. In addition, the wounded persons suffered from sleep problems. Therefore, early and continuous interventions of public institutions and public health specialists are needed for the wounded persons with chronic psychiatric disorders.

• Biomolecules & Therapeutics
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• 제25권6호
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• pp.625-633
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• 2017

Sphingosylphosphorylcholine (SPC) is one of the bioactive phospholipids that has many cellular functions such as cell migration, adhesion, proliferation, angiogenesis, and $Ca^{2+}$ signaling. Recent studies have reported that SPC induces invasion of breast cancer cells via matrix metalloproteinase-3 (MMP-3) secretion leading to WNT activation. Thrombospondin-1 (TSP-1) is a matricellular and calcium-binding protein that binds to a wide variety of integrin and non-integrin cell surface receptors. It regulates cell proliferation, migration, and apoptosis in inflammation, angiogenesis and neoplasia. TSP-1 promotes aggressive phenotype via epithelial mesenchymal transition (EMT). The relationship between SPC and TSP-1 is unclear. We found SPC induced EMT leading to mesenchymal morphology, decrease of E-cadherin expression and increases of N-cadherin and vimentin. SPC induced secretion of thrombospondin-1 (TSP-1) during SPC-induced EMT of various breast cancer cells. Gene silencing of TSP-1 suppressed SPC-induced EMT as well as migration and invasion of MCF10A cells. An extracellular signal-regulated kinase inhibitor, PD98059, significantly suppressed the secretion of TSP-1, expressions of N-cadherin and vimentin, and decrease of E-cadherin in MCF10A cells. ERK2 siRNA suppressed TSP-1 secretion and EMT. From online PROGgene V2, relapse free survival is low in patients having high TSP-1 expressed breast cancer. Taken together, we found that SPC induced EMT and TSP-1 secretion via ERK2 signaling pathway. These results suggests that SPC-induced TSP-1 might be a new target for suppression of metastasis of breast cancer cells.

• 한국군사과학기술학회지
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• 제7권2호
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• pp.81-87
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• 2004

Semiconductor thick film gas sensors based on tin oxide are fabricated and their gas response characteristics are examined for four simulant gases of chemical warfare agent (CWA)s. The sensing materials are prepared in three different sets. 1) The Pt or Pd $(1,\;2,\;3\;wt.\%)$ as catalyst is impregnated in the base material of $SnO_2$ by impregnation method.2) $Al_2O_3\;(0,\;4,\;12,\;20\;wt.\%),\;In_2O_3\;(1,\;2,\;3\;wt.\%),\;WO_3\;(1,\;2,\;3\;wt.\%),\;TiO_2\;(3,\;5,\;10\;wt.\%)$ or $SiO_2\;(3,\;5,\;10\;wt.\%)$ is added to $SnO_2$ by physical ball milling process. 3) ZnO $(1,\;2,\;3,\;4,\;5\;wt.\%)$ or $ZrO_2\;(1,\;3,\;5\;wt.\%)$ is added to $SnO_2$ by co-precipitation method. Surface morphology, particle size, and specific surface area of fabricated sensing films are performed by the SEM, XRD and BET respectively. Response characteristics are examined for simulant gases with temperature in the range 200 to $400^{\circ}C$, with different gas concentrations. These sensors have high sensitivities more than $50\%$ at 500ppb concentration for test gases and also have shown good repetition tests. Four sensing materials are selected with good sensitivity and stability and are fabricated as a sensor array A sensor array Identities among the four simulant gases through the principal component analysis (PCA). High sensitivity is acquired by using the semiconductor thick film gas sensors and four CWA gases are classified by using a sensor array through PCA.

• 대한불안의학회지
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• 제8권1호
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• pp.16-21
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• 2012

Objectives : The current study investigated the personality characteristics of the sexual offender using the Minnesota Multiphasic Personality Inventory (MMPI) test commonly used in clinical medicine. Methods : 30 sexual offenders (30 male) completed the Korean version of the MMPI. 20 of the 30 sexual offenders were patients with paraphilia. The sexual offenders had been admitted to the Institute of Forensic Psychiatry Ministry of Justice, Gongju, for sexual violence. Results : The MMPI scores of the sexual offenders were significantly higher on the psychopathic deviate (Pd), masculinity-feminity (Mf), paranoia (Pa), psychasthenia (Pt), schizophrenia (Sc) than the comparison group. And The MMPI scores of the sexual offenders had higher values for hypochondriasis (Hs), depression (D), hysteria (Hy), hypomania (Ma), social introversion (Si) than the comparison group, but were not significantly higher. Conclusion : Sexual offenders may be antisocial, paranoid, schizoid or psychasthenic. They seem to have psychotic psychopathology rather than neurotic psychopathology. These results suggested that the psychopatholgy of sexual offenders may be different to the control group.

• 대한건축학회논문집:계획계
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• 제33권12호
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• pp.105-112
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• 2017

Through various media, damage of both smoking and second-hand smoking has been recognized, and brought global scale of interest in antismoking. In Korea, government has tightened regulations of smoking in non-smoking zone since December, 1980, and after National Health Promotion Act in 1995, non-smoking zone has been gradually expanded. On the other hand, there were law suits to find those regulation towards smokers are either unconstitutional or not for 4 times. In this current state, people need smoking area to prevent second-hand smoking and to consider smokers in multi-unit dwelling. Main purpose of this research is to plan smoking spaces based on various typology of multi-dwelling plan for protection of both smokers and non-smokers' right. The research group collected and analyzed the smoking behaviors in various multi-unit dwelling types such as flat type, tower type, hybrid type and others. Based on those data, the group found three phenomena. First, there are internal regulations in multi-unit dwelling to make non-smoking zone based on National Health Promotion Act and resident representative meeting decision. Second, main smoking activities are occurring at major traffic line and entrances. Third, smoking inside of multi-unit dwelling complex causes second-hand smoking to residents live in $1^{st}$ floor and when they enter. Therefore, one can achieve both smokers' and non-smokers' protection of right by creating a designated smoking space near main entrances of multi-unit dwelling complex to consider smokers' and prevents second-hand smoking by using shaft space, which is in core space, to ventilate tobacco smoke through roof.

• 대한건축학회논문집:계획계
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• 제34권8호
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• pp.77-86
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• 2018

The purpose of this study is to examine the condition of the architectural location and the history of Yeongbojeong and investigate the motivation which formed the YuSik space and the poetical circles of it. And another purpose of the study is to discover how Yeongbojeong, located in a military camp in Western coast, displayed the poetic scenery so to be the poetic summer house among so many Nujeongs in Joseon. Yeongbojeong is currently located in Soseong-ri, Ocheon-myeon, Boryeong City, Chungnam. Yeongbojeong has the biggest size among all the summer houses in the country with 24 architectural rooms. And Yeongbojeong is not only the summer house in which people could simply see its scenery, but beyond the poetic space of Joseon, it was the unique poetic summer house which enables poets and calligraphers to enjoy imaginary freedom. The time range of the study was from the building of Yeongbojeong in 1504 to its demolish in 1901, and its space range was taken focused on the particular scenery which was seen from around Yeongbojeong. To investigate the architectural aspect of Yeongbojeong, the existing records about the summer houses, "OcheonGoonJi" and the landscape paintings etc. were referred. The travel records and poems in the work collection of the famous poets who explored Yeongbojeong were referred to examine the formation of the Yeongbojeong poetical circles. The result of the study shows the process that Yeongbojeong had become the famous national place with scenery and how it became the most visited YuSik space. And this study figured out the full account of Yeongbojeong poetical circles which had been formed by so many poets of the country for 4 hundred years.

• 대한건축학회논문집:계획계
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• 제35권2호
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• pp.105-114
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• 2019

Yeongbojeong was a famous pavilion of Joseon, which was located in Chungcheong-Suyeong, Ocheon-myeon, Boryeong-si. It was an open place for poets and calligraphers to freely visit during 400 years. Until today, it is widely said that Yeongbojeong was newly built by LeeRyang in 1504, who was Sugunjeoldosa(naval forces officer). The researcher investigated Gyujanggak to search Lee Maenghyu's "Asulrok-Book", and it was determined that Yeongbojeong was created by Gang Soon, 36 years earlier than LeeRyang. Accordingly, this research aimed at determining the appropriateness of the creation of Yeongbojeong. "Asulrok-Book", which said, "Yeongbojeong was Wonjeong(園亭) of Gang Soon during Yejong's reign", however this research could settle imperfection of Geungeo Gimun which was not clearly explained. Yeongbojeong was created due to Gang Soon's Wonjeong in 1468, however he was also a traitor and was executed, so that nobody wanted to mention about it. Therefore, it was determined that no phrase related to the record of creation of Yeongbojeong by Gang Soon out of lots of Yongbojeong Jeyoungsi and Gimun. Lee Maenghyu was a brave scholar who recorded 'Yeongbojeong was Gang Soon's Wonjeong' confidently, breaking such taboo. This research intended to clearly investigate the year of foundation of Yeongbojeong and establish the history of Chungcheong-Suyeong by thoroughly analyzing newly discovered "Asulrok-Book", existing "Ocheong GoonJi", and "Chungcheongsaryejib" and considering the fact relevance. As the result of this research, it was determined that Yeongbojeong was Gang Soon's Wonjeong through "Asulrok-Book". The point that Yeongbojeong was forfeited reverted to neighboring Chungcheong-Suyeong after Gang Soon was executed due to his participation in a conspiracy also was a newly discovered fact. LeeRyang, Sugunjeoldosa, can be said a brave commander, since he put up the original Yeongbojeong instead of abandoning the name Yeongbojeong of Gang soon. So far, Yeongbojeong is widely accepted as 'new construction of Yeongbojeong by LeeRyang in 1504'. However, since the record of Gang Soon's Wonjeong was discovered, and as the result of diversified analysis and examination, it is proper to correct the foundation year of Yeongbojeong as 1468.

• 대한건축학회논문집:계획계
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• 제35권1호
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• pp.139-147
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• 2019

The purpose of this paper is to analyze the linkage between Si-Jeong-Chon (municipalities of Japan) catastrophic disaster recovery-regeneration plan (DRRP) and regional comprehensive plan (RCP), each of which was made after the Great East Japan Earthquake, and to make implications on establishment of a disaster recovery plan of Korea. In order to explore the relationship between the two plans, the DRRPs and RCPs of 30 municipalities are collected and categorized according to their characteristics. The results show that DRRPs of the municipalities are not always in a consistent form and contents because regulations and guidelines on DRRP are not definite and specific. The relationship between DRRP and RCP in terms of its form and contents can be divided into 3 categories; Reflective Type (RT), Complementary Type (CT), and Substitutive Type (ST). The 22 RT plans as the majority of those DRRPs appear to reflect the development strategies of municipalities' RCPs. Both 3 CT plans and 5 ST plans seem to be integrated with RCPs in some way. The plan in CT of a municipality supplements its RCP by adding some new strategies to its recovery section and the plan in ST of a municipality appears to replace its RCP. Finally the influential factors which are considered to determine linkage type are identified as remaining time which is legally required to re-establish its RCP, the extent of damage, and socioeconomic condition changes.

• The Korean Journal of Physiology and Pharmacology
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• 제26권5호
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• pp.389-396
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• 2022

The increased expression of receptors for advanced glycation end-product (RAGE) is known as a key player in the progression of vascular remodeling. However, the precise signal pathways regulating RAGE expression in vascular smooth muscle cells (VSMCs) in the injured vasculatures are unclear. Given the importance of mitogen-activated protein kinase (MAPK) signaling in cell proliferation, we investigated the importance of MAPK signaling in high-mobility group box 1 (HMGB1)-induced RAGE expression in VSMCs. In HMGB1 (100 ng/ml)-stimulated human VSMCs, the expression of RAGE mRNA and protein was increased in association with an increase in AGE-induced VSMC proliferation. The HMGB1-induced RAGE expression was attenuated in cells pretreated with inhibitors for ERK (PD98059, 10 μM) and p38 MAPK (SB203580, 10 μM) as well as in cells deficient in ERK and p38 MAPK using siRNAs, but not in cells deficient of JNK signaling. In cells stimulated with HMGB1, the phosphorylation of ERK, JNK, and p38 MAPK was increased. This increase in ERK and p38 MAPK phosphorylation was inhibited by p38 MAPK and ERK inhibitors, respectively, but not by JNK inhibitor. Moreover, AGE-induced VSMC proliferation in HMGB1-stimulated cells was attenuated in cells treated with ERK and p38 MAPK inhibitors. Taken together, our results indicate that ERK and p38 MAPK signaling are involved in RAGE expression in HMGB1-stimulated VSMCs. Thus, the ERK/p38 MAPK-RAGE signaling axis in VSMCs was suggested as a potential therapeutic target for vascular remodeling in the injured vasculatures.