• Title/Summary/Keyword: Pcb

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Biodegradation Pathways of Polychlorinated Biphenyls by Soil Fungus Aspergillus niger (Polychlorinated Biphenyl의 토양 미생물 Aspergillus niger에 의한 생분해 경로)

  • Kim, Chang-Su;Lim, Do-Hyung;Keum, Young-Soo
    • The Korean Journal of Pesticide Science
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    • v.20 no.1
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    • pp.7-13
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    • 2016
  • As of many organochlorine pesticides, polychlorinated biphenyls are ubiquitous organic contaminants, which can be found in the most environmental matrices. Their toxic effects include endocrinedisrupting activity. Most researches with these toxicants performed with mixtures of congeners, namely Aroclor and related study has been done in complex environmental matrix, rather than single biosystems or pure congeners. 5 congeners were synthesized and their fates in pure microbial culture (Aspergillus niger) were determined in this study. Among biphenyl and synthetic congeners, biphenyl, PCB-1 (2-chlorobiphenyl), and PCB-3 (4-chlorobiphenyl) were rapidly transformed to hydrophilic metabolites, followed by PCB-38 (3,4,5-trichlorobiphenyl), while the degradation of PCB-126 (3,3',4,4',5-pentachlorobiphenyl) was not observed. The amounts of transformation for biphenyl, PCB-1, PCB-3, and PCB-38 were 65, 38, 52, and 2% respectively. The major metabolites of the above congeners were identified as mono- and di-hydroxy biphenyls, which are known to give adverse endocrinological effects.

Effects of 3,3′,4,4′,5-pentachloro biphenyl (PCB126) on the Expression the Tight Junction Genes in Cultured Mouse Neonatal Testis

  • Gye, Myeong-Chan;Fukuzawa, Noriho Honda;Ohsako, Seiichiro
    • Proceedings of the Korean Society of Developmental Biology Conference
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    • 2003.10a
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    • pp.63-63
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    • 2003
  • In an effort to uncover the spermatogenic impairment by the polychlorinated biphenyls (PCBs), the expression of tight junctions (TJs) genes important for the formation of the blood testis barrier (BTB) were examined following the 3,3',4,4',5-pentachloro biphenyl (PCB126) treatment in cultured neonatal testis in mice. At 4 days (D4) after 10 and 100 nM PCB126 treatment the expression of claudin-11 was significantly increased when compared with vehicle control. In contrast no difference in occludin and claudin-1 expression was found among the experimental group. On D8, 100 nM PCB126 significantly increased the expression of claudin-11 but not occludin and claudin-1. 1 uM PCB126 treatment significantly decreased expressions of occludin and ciaudin -1, suggesting the general toxic effect on the Sertoli cell. Because PCB126 does not alter the proliferative activity of spermatogenic cells and Sertoli cells in neonatal testis, it is likely that increase in the expression of claudin-11 by low dose of PCB126 may attribute to the alteration of the Sertoli cells differentiation in testis. It also emphasized that PCB126 might have differentially affected the transcription of TJ genes in Sertoli cells. In conclusion, this result suggests that the structure of TJ may be targeted by PCB126 in neonatal testis in mice and that co-PCB is potentially harmful to spermatogenesis by alteration of the development of BTB.

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A study on Source Stability Design Method by Power Integrity Analysis (전원무결성 해석에 의한 PCB 전원안정화 설계기법 연구)

  • Chung, Ki-Hyun;Jang, Young-Jin;Jung, Chang-Won;Kim, Seong-Kweon
    • The Journal of the Korea institute of electronic communication sciences
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    • v.9 no.7
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    • pp.753-759
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    • 2014
  • This paper introduces the reduction design technique of the resonance phenomenon of the inner PCB based on power integrity from the analysis about the inner power supply line generating RLC resonance. With the technique, the resonant frequency resulted from the structural characteristics of the PCB can be analyzed and allows to predict and the capacitor for resonance phenomenon reduction can be decided as a decoupling capacitor. From the simulation result, it was confirmed that the PCB's resonance phenomenon reduction design technique should have the reduction effect in the inner motherboard of the industrial controller. This research will be contributed to the improvement of the safety of a PDN (Power Delivery Network) structure in the layout design technique of the PCB.

Estimation of Indent Fracture due to the Moving Process of a Pin on PCB Plate (PCB 판에 대한 핀의 이동 공정에 따른 압입파괴 평가)

  • Kim, Young-Choon;Kim, Choon-Sik;Lee, Hee-Sung;Cho, Jae-Ung
    • Journal of the Korea Academia-Industrial cooperation Society
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    • v.15 no.12
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    • pp.6967-6972
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    • 2014
  • Assembly using a bolt and nut, and rivet or pin have been used widely for forming mechanical joints. The indent method is an easier process than other manufacturing techniques and the toughness of the material is excellent. On the other hand, there are many cases in which the cracks occur on the manufacturing process as the indent method. Therefore, two kinds of models, in which a pin goes into and out PCB plate in this study were developed using the CATIA program and finite element methods were performed using the ANSYS program. When a pin was passed through a PCB plate in cases 1 and 2, the maximum loads applied to the PCB plate were 79.708N and 90.277N, respectively. When the PCB plate came out of the pin in cases 1 and 2, the maximum loads were 63.783N and 33.75N, respectively. The damage prevention and durability can be improved by applying the study results to the design of real indentation.

Cloning and Expression of pcbCD Genes in Escherichia coli from Pseudomonas sp. DJ-12 (Pseudomonas sp. DJ-12의 pcbCD 유전자의 클로닝과 Escherichia coli에서의 발현)

  • Kim, Chi-Kyung;Sung, Tae-Kyung;Nam, Jung-Hyun;Kim, Chang-Young;Lee, Jae-Koo
    • Korean Journal of Microbiology
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    • v.32 no.1
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    • pp.40-46
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    • 1994
  • The pcb genes of Pseudomonas sp. DJ-12 coded for the catabolism of polychlorinated biphenyl (PCBs) and biphenyl. The products of the pcbCD genes were 2,3-dihydroxy-4'-chlorobiphenyl dioxygenase and meta-cleavage product (MCP) hydrolase, which acted on degradation of 2,3-dihydroxy-4'-chlorobiphenyl to 4-chlorobenzoate. The pcbCD genes were cloned in E. coli XLl-Blue, and then the pcbD gene was further subcloned. As a metabolite transformed from 2,3-dihydroxybiphenyl by the cloned cell of E coli CU103, benzoate was detected by the resting cell assay. The enzyme activities of 2,3-dihydroxybiphenyl dioxygease and MCP hydrolase produced in the cloned cells E. coli CU103 and CU105 were about 17 and 3 times higher than those of Pseudomonas sp. DJ-12, respectively.

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Viscosity Change of Al2O3-SiO2-CaO Slag System with Used Electronic Scrap (산화처리된 PCB 스크랩을 첨가한 Al2O3-SiO2-CaO 3성분계 슬래그의 점도)

  • Kwon, Eui-Hyuk;Han, Sin-Suk;Ji, Jae-Hong;Han, Jeong-Whan;You, Byung-Don;Kim, Byung-Soo;Lee, Jae-Chun
    • Korean Journal of Materials Research
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    • v.13 no.4
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    • pp.239-245
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    • 2003
  • In order to explore the possibility to extract precious metals from PCB(Printed Circuit Board) scrap by gravity separation, a high temperature melting process was adopted, from the recycling view point, to investigate the influence of viscosity on A1$_2$$O_3$-CaO$-SiO_2$ slag system composed of PCB scrap. For optimizing the pre-treatment process of PCB scrap, an experimental condition for the complete calcination and oxidation of organic materials in PCB scrap was established and a quantitative analysis of oxidized PCB scrap was also carrie out. It was found that 6 hours were enough for the complete oxidation of PCB scrap at 1273 K in an atmosphere condition. A slag, l5wt%$A1_2$$O_3$-45wt%CaO-40wt%SiO$_2$, was chosen as a basic slag composition which is determined based on the quantitative analysis of PCB scrap. Viscosities were measured in slag systems both made from pure fluxes and from PCB scrap with additional fluxes. Slag viscosities composed of pure fluxes were measured to be 5.29 poise and 30.52 poise at temperatures of 1773 and 1573 K, whereas that of PCB scrap with additional fluxes were 3.37 poise and 69.89 poise, respectively.

Design for High-Efficient Passive Optical PCB Interconnection by Using Built-in Lens Structure (렌즈 일체형 광도파로를 이용한 고효율 수동 광 PCB 접속 구조 설계)

  • Kim, Dong-Min;Lee, Tae-Kyoung;Lee, Tae-Ho;Jeong, Myung-Yung
    • Journal of the Microelectronics and Packaging Society
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    • v.19 no.2
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    • pp.47-53
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    • 2012
  • Recently, by the increasing of data transmission rates, PCB is required high-speed data transmission rates and thin packaging. So optical PCB which is the combination of electrical layer and optical layer can be one of the solution to overcome the limitations of conventional electrical PCB. The most important factor in the implementation of optical PCB is optical interconnection. So the research on high-efficiency and passive alignment has been active. In this paper, we suggest built-in lens pluggable waveguide and we simulate its coupling efficiency and structural stability. Optical simulation results show that the proposed structure has higher efficiency than no lens structure about 1.86 times in transmitter and about 1.42 times in receiver. In structure simulation, inner lens has no damage in desorption process. Therefore, we shown that the proposed structure has a high coupling efficiency and structural stability.

Design of 4-Layer PCB Considering EMC for Automotive Bluetooth Speaker (차량용 블루투스 스피커를 위한 EMC를 고려한 4층 PCB 설계)

  • Yoon, Ki-Young;Kim, Boo-Gyoun;Lee, Seongsoo
    • Journal of IKEEE
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    • v.25 no.4
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    • pp.591-597
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    • 2021
  • This paper proposes an EMC-aware PCB design method to reduce electromagnetic emission, where trace length and teturn path of critical signal are shortened by changing chip location and trace layout on the PCB, while additional filters or decoupling capacitors are not required. In the proposed method, signal velocity is calculated for various signals on the PCB. Critical signal with the fastest signal velocity is determined and its return path is shortened as much as possible by placing chip location and trace routing first. Return path of critical signal should be carefully designed not to have discontinuity. Power plane and ground plane should be carefully designed not to be divided, since these planes are the reference of return path. The proposed method was applied to automotive directional Bluetooth speaker which failed to pass CISPR 32 and CISPR 25 EMC tests. Its PCB was redesigned based on the proposed method and it easily passed the EMC tests. The proposed method is useful to EMC-sensitive electronic equipments.

The Analysis of Thermal & Optical Properties in LED Package by the PCB structure and via hole formation (PCB 구조와 via hole 구성에 따른 LED 패키지의 열적 광학적 특성 분석)

  • Lee, Se-Il;Lee, Seung-Min;Yang, Jong-Kyung;Park, Hyung-Jun;Park, Dae-Hee
    • Proceedings of the Korean Institute of Electrical and Electronic Material Engineers Conference
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    • 2009.06a
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    • pp.297-298
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    • 2009
  • 대부분의 반도체 소자의 고장 원인은 85%정도가 열로 인한 것이며, 고출력 LED는 인가된 에너지의 20%정도의 광으로 출력되며 나머지 80%가 열로 전환된다. 본 논문에서는 PMS-50과 KEITHLEY 2430을 이용하여 PCB 구조와 Via hole 구성에 따른 LED 패키지의 열적 광학적 특성을 분석하였다. 0.6mm의 Via hole을 가진 FR4 PCB의 열특성이 가장 우수하였으며, Via hole 0.6mm FR4 PCB의 경우 McPCB에 상응하는 광출력 특성을 보였다.

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Biodegradation of PCBs into Contaminated Dredged Sediments (오염퇴적물에 함유된 PCBs의 생물분해 특성)

  • 안재환;김소정;지재성;배우근;황병기
    • Journal of Soil and Groundwater Environment
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    • v.9 no.2
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    • pp.41-47
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    • 2004
  • This research in the degradation of polychlorinated biphenyls(PCB) has focussed on the use of experimental enrichment cultures to obtain PCB-deading communities and identification of PCB-degrading bacteria accor야ng to pure culture. During 180 days, enrichment culture was performed to obtain PCB-degrading bacteria and initial concentration was injected 1.6 ppm,0.7 ppm, respectively. After 180 days of enrichment culture, PCBs was removed 80-87% and 57-71%. Biodegradation of PCBs was studied according to dominated PCB-degrading bacteria. Biodegraddation of PCBs was 80% in initial concentration of PCBs for 20days, enrichment cultured PCB-degrading bacteria was isolated by pure culture and it was verified to Pseudoxanthomonas sp.