• Journal of Oriental Neuropsychiatry
• /
• v.21 no.2
• /
• pp.157-169
• /
• 2010

Objectives : The aim of this study was to investigate the characteristics of Hwa-Byung(HB) patients, draw the clinical key issue of HB, and provide the data as the basis for development of Clinical Guideline of HB. Methods : The study participants included 151 subjects who thought they have HB in 9 site. For all patients, we used HB epidemiologic study protocol, which include the Korean version of the Structured Clinical Interview for the DSM-IV Diagnosis (SCID-1), diagnostic criteria of HB, symptoms check list, pattern identification tool, psychiastric psychological questionaires, and Framingham coronary risk score(FRS). Results : 1. For all participants, 62 % of subjects were diagnosed with HB. These patients had comorbid DSM-IV diagnoses with depression (65%) and anxiety (27%). But 22% of these patients had only HB. 2. HB patients had various physical symptoms when visiting, were diagnosed as various disease, and undergone medical treatments in the past. Among the diagonsed illness, Gastronitestinal diseases (51.6%), psychiatric disorders(40.9%), endocrine diseases(39.8%) were being the most frequent. The frequent physical symptoms of HB patients reported were chest discomfort, head ache, pallpitation, frequent sigh, amnesia, shoulder pain, dry mouth, eye fatigue etc. 3. HB patients had high scores in psychologic questionares, CES-D, STAI, and STAXI. It means that HB patients might be low-level emotional stability. 4. Participant had negative opinion about the treatment of HB that it would be difficult or impossible (65.7%), but they neglect the need of medical treatment. Conclusions : This result shows that HB is comorbid with various psychiatric disease, but it has different physio-psychological symptoms from others and there were only HB patients. so we identify HB is independent disease. Moreover, there were the wrong perception of HB which blocking treatments. These problems support that the neccesity of development of Clinical Guideline of HB.

• Korean Journal of Ecology and Environment
• /
• v.36 no.3 s.104
• /
• pp.369-374
• /
• 2003

Nonylphenol (NP), an well known aquatic contaminant, has been known to induce abnormalities in various aquatic animals. In an effort to develop proteome in the study of aquatic contamination of NP and its impact on the amphibia, protein changes in liver tissues of Korean red bellied frog, Bombina orientalis was investigated following the NP exposure. NP was administered intraperitoneally to male B. orientalis at 10 mg/kg body weight. At 48 and 96h after the treatment, the frog livers were sampled, and the protein fraction was separated using two dimensional gel electrophoresis (2D/E) and visualized with Coomassie brilluant blue staining. The 2D/E Images of the tissue from the animals treated with NP showed marked changes of protein spots (about 20% of total protein spots). Analysis of the 50-60 separated spots allowed identification of the major protein changes in the overall pattern for the stressor (NP) by time (0,48 and 96 h). At 48h after treatment, 8 spots were increased and 12 spots were reduced. Then, at 96h after treatment, 10 spots were increased and 8 spots were reduced. In total, approximately 29% of liver proteins showed the altered expression following the NP treatment. It is suggested that protein expression was repressed by blocking of certain metabolisms at 48 hand induced by the synthesis of new proteins for adaptation at 96 h following NP exposure. This application for 2D/E analysis may show promise in searching biomarkers for environmental proteomics in amphibians.

• Korean Journal of Ecology and Environment
• /
• v.47 no.2
• /
• pp.127-134
• /
• 2014

Organic carbon and nitrogen stable isotope ratios of particulate organic matter (POM) were determined at 7 stations (6 stations in Han river and Paldang dam outflow station) from May to October 2013, in order to understand the origin of POM at the Han river. ${\delta}^{13}C$ values of POM in four stations showed significant seasonal changes season, but ${\delta}^{15}N$ values were enriched in around Kyeongan stream (K). POC, PN and Chl-a concentration showed a similar seasonal pattern in Kyeongan stream, with an apparent decrease from July to August. POC and PN concentration has a higher correlation with Chl-a concentration in Kyeongan stream (K). ${\delta}^{13}C$ and ${\delta}^{15}N$ values of POM has a lighter value during lower Chl-a concentration peroid, compared to other seasons. Our results revealed that Kyeongan stream (K) seemed to be influenced by substantial amount of organic manure or fertilizer input in 2013, compared to the previous year (2012). These results suggest that the analysis of stable isotope ratios is a simple but useful tool for the identification of organic matter origin in aquatic environments.

• Journal of the Institute of Electronics Engineers of Korea SP
• /
• v.40 no.5
• /
• pp.378-387
• /
• 2003

Digital watermarking is the technique that embeds invisible signalsincluding owner identification information, specific code, or pattern into multimedia data such as image, video and audio. Watermarking techniques can be classified into two groups; robust watermarking and fragile(semi-fragile) watermarking. The main purpose of the robust watermarking is the protection of copyright, whereas fragile(semi-fragile) watermarking prevents image or video data from illegal modifications. To achieve this goal watermark should survive from unintentional modifications such as random noise or compression, but it should be fragile for malicious manipulations. In this paper, an invertible semi-fragile watermarkingalgorithm for authentication and detection of manipulated location in MPEG-2 bit-stream is proposed. The proposed algorithm embeds two kinds of watermarks, which are embedded into quantized DCT coefficients. So it can be applied directly to the compressed bit-stream. The first watermark is used for authentication of video data. The second one is used for detection of malicious manipulations. It can distinguish transcodingin bit-stream domain from malicious manipulation and detect the block-wise locations of manipulations in video data. Also, since the proposed algorithm has an invertible property, recovering original video data is possible if the watermarked video is authentic.

• Journal of the Institute of Electronics Engineers of Korea SP
• /
• v.48 no.4
• /
• pp.107-115
• /
• 2011

In this paper, we proposed fast and efficient algorithm for detecting near-duplication based on content based retrieval in large scale video database. For handling large amounts of video easily, we split the video into small segment using scene change detection. In case of video services and copyright related business models, it is need to technology that detect near-duplicates, that longer matched video than to search video containing short part or a frame of original. To detect near-duplicate video, we proposed motion distribution and frame descriptor in a video segment. The motion distribution descriptor is constructed by obtaining motion vector from macro blocks during the video decoding process. When matching between descriptors, we use the motion distribution descriptor as filtering to improving matching speed. However, motion distribution has low discriminability. To improve discrimination, we decide to identification using frame descriptor extracted from selected representative frames within a scene segmentation. The proposed algorithm shows high success rate and low false alarm rate. In addition, the matching speed of this descriptor is very fast, we confirm this algorithm can be useful to practical application.

• Clinical and Experimental Reproductive Medicine
• /
• v.29 no.2
• /
• pp.117-127
• /
• 2002

Objective: This study was to investigate the generation of the functional neuron derived from human embryonic stem (hES, MB03) cells on in vitro neural cell differentiation system. Methods: For neural progenitor cell formation derived from hES cells, we produced embryoid bodies (EB: for 5 days, without mitogen) from hES cells and then neurospheres (for $7{\sim}10$ days, 20 ng/ml of bFGF added N2 medium) from EB. And then finally for the differentiation into mature neuron, neural progenitor cells were cultured in i) N2 medium only (without bFGF), ii) N2 supplemented with 20 ng/ml platelet derived growth factor-bb (PDGF-bb) or iii) N2 supplemented with 5 ng/ml brain derived neurotrophic factor (BDNF) for 2 weeks. Identification of neural cell differentiation was carried out by immunocytochemistry using $\beta_{III}$-tubulin (1:250), MAP-2 (1:100) and GFAP (1:500). Also, generation of functional neuron was identified using anti-glutamate (Sigma, 1:1000), anti-GABA (Sigma, 1:1000), anti-serotonin (Sigma, 1:1000) and anti-tyrosine hydroxylase (Sigma, 1:1000). Results: In vitro neural cell differentiation, neurotrophic factors (PDGF and BDNF) treated cell groups were high expressed MAP-2 and GFAP than non-treated cell group. The highest expression pattern of MAP-2 and $\beta_{III}$-tubulin was indicated in BDNF treated group. Also, in the presence of PDGF-bb or BDNF, most of the neural cells derived from hES cells were differentiated into glutamate and GABA neuron in vitro. Furthermore, we confirmed that there were a few serotonin and tyrosine hydroxylase positive neuron in the same culture environment. Conclusion: This results suggested that the generation of functional neuron derived from hES cells was increased by addition of neurotrophic factors such as PDGF-bb or BDNF in b-FGF induced neural cell differentiation system and especially glutamate and GABA neurons were mainly produced in the system.

• Journal of Ginseng Research
• /
• v.40 no.2
• /
• pp.97-104
• /
• 2016

Background: Rhizobacteria play an important role in plant defense and could be promising sources of biocontrol agents. This study aimed to screen antagonistic bacteria and develop a biocontrol system for root rot complex of Panax notoginseng. Methods: Pure-culture methods were used to isolate bacteria from the rhizosphere soil of notoginseng plants. The identification of isolates was based on the analysis of 16S ribosomal RNA (rRNA) sequences. Results: A total of 279 bacteria were obtained from rhizosphere soils of healthy and root-rot notoginseng plants, and uncultivated soil. Among all the isolates, 88 showed antagonistic activity to at least one of three phytopathogenic fungi, Fusarium oxysporum, Fusarium solani, and Phoma herbarum mainly causing root rot disease of P. notoginseng. Based on the 16S rRNA sequencing, the antagonistic bacteria were characterized into four clusters, Firmicutes, Proteobacteria, Actinobacteria, and Bacteroidetesi. The genus Bacillus was the most frequently isolated, and Bacillus siamensis (Hs02), Bacillus atrophaeus (Hs09) showed strong antagonistic activity to the three pathogens. The distribution pattern differed in soil types, genera Achromobacter, Acidovorax, Brevibacterium, Brevundimonas, Flavimonas, and Streptomyces were only found in rhizosphere of healthy plants, while Delftia, Leclercia, Brevibacillus, Microbacterium, Pantoea, Rhizobium, and Stenotrophomonas only exist in soil of diseased plant, and Acinetobacter only exist in uncultivated soil. Conclusion: The results suggest that diverse bacteria exist in the P. notoginseng rhizosphere soil, with differences in community in the same field, and antagonistic isolates may be good potential biological control agent for the notoginseng root-rot diseases caused by F. oxysporum, Fusarium solani, and Panax herbarum.

• Science of Emotion and Sensibility
• /
• v.23 no.1
• /
• pp.17-28
• /
• 2020

Lowering observers' view-height may increase the amount of occlusion across objects in a visual scene and prevent the accurate identification of the objects in the scene. Based on this possibility, memory stimuli in relation to their expected views from different heights were displayed in this study. Thereafter, visual short-term memory (VSTM) performance for the stimuli was measured. In Experiment 1, the memory stimuli were presented on a grid-background drawn according to linear perspectives, which varied across observers' three different view-heights (high, middle, and low). This allowed the participants to remember both the color and position of each memory stimulus. The results revealed that testing participants' VSTM performance for the stimuli under a different memory load of two set-sizes (3 vs. 6) demonstrated an evident drop of performance in the lowest view-height condition. In Experiment 2, the performance for six stimuli with or without the grid-background was tested. A similar pattern of performance drop in the lowest condition as in Experiment 1 was found. These results indicated that different view-heights of an observer can change the amount of occlusion across objects in the visual field, and the sensory interference driven by the occlusion may further influence VSTM performance for those objects.

• Journal of Plant Biotechnology
• /
• v.45 no.1
• /
• pp.9-16
• /
• 2018

The advent of available DNA barcoding technology has been extensively adopted to assist in the reference to differentiate the origin of various medicinal plants species. However, this technology is still far behind the curve of technological advances to be applied in a practical manner in the market to authenticate the counterfeit components or detect the contamination in the admixtures of medicinal plant species. Recently, a high resolution melting curve analysis technique was combined with the procedure of DNA barcoding (Bar-HRM) to accomplish this purpose. In this review, we tried to summarize the current development and bottleneck of processing related to the Bar-HRM technology for the practical application of medicinal plant species' differentiation in a viable global market. Although several successful results have been reported, there are still many obstacles to be resolved, such as limited number of DNA barcodes and single nucleotide polymorphisms, in particular, only one DNA barcode, internal transcribed sequence (ITS) of ribosomal DNA has been reported in the available nuclear genome. In addition, too few cases have been reported about the identification of counterfeit or contamination with processed medicinal plant products, in particular specifically the case of technology based infusion, jam and jelly products and components in which it is noted that DNA can be thereby degraded during the processing of these products and components.

• The Journal of Korean Institute of Electromagnetic Engineering and Science
• /
• v.20 no.6
• /
• pp.535-543
• /
• 2009

This paper describes a design for RFID(Radio Frequency Identification) tag antenna using license plate attached the vehicle bumper in 900 MHz band. The proposed tag antenna size which is located on upper center position of a vehicle license plate is 162.5${\times}$40${\times}$1 mm$^3$. A resonant frequency of design antenna and the bandwidth which has return loss of -10 dB below are 900 MHz and about 720 MHz(640${\sim}$1,360 MHz), respectively. The commercial chip impedance considered on design was 16- j131 ${\Omega}$ and the complex conjugate impedance of chip was used as input impedance of tag antenna. The measured return loss and radiation pattern were agreed well with the calculated results. The measured readable range of the proposed tag antenna designed on only the vehicle license plate was 11.5 m. Moreover, its range of the fabricated tag antenna that the license plate and the vehicle bumper were fixed by volt and nut was observed 10.4 m. These measured readable range showed about 5 m above far distance more than the average readable range of commercial tag antenna.