• Title/Summary/Keyword: Pathogenic Bacteria

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Antibacterial Effect of Eucalyptus Oil, Tea Tree Oil, Grapefruit Seed Extract, Potassium Sorbate, and Lactic Acid for the development of Feminine Cleansers

  • Yuk, Young Sam
    • International Journal of Internet, Broadcasting and Communication
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    • v.13 no.2
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    • pp.82-92
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    • 2021
  • Purpose: It has been reported that the diversity and abundance of microbes in the vagina decrease due to the use of antimicrobial agents, and the high recurrence rate of female vaginitis due to this suggests that a new treatment is needed. Methods: In the experiment, we detected that 10% potassium sorbate solution, 1% eucalyptus oil solution, 1% tea tree oil solution, 400 µL/10 mL grapefruit seed extract solution, 100% lactic acid, 10% acetic acid solution, and 10% lactic acid solution were prepared and used. After adjusting the pH to 4, 5, and 6 with lactic acid and acetic acid in the mixed culture medium, each bacterium was inoculated into the medium and incubated for 72 h at 35℃. Incubate and 0 h each. 24 h. 48 h. The number of bacteria was measured after 72 h. Results: In the mixed culture test between lactic acid bacteria and pathogenic microorganisms, lactic acid bacteria showed good results at pH 5-5.5. Potassium sorbate, which has varying antibacterial activity based on the pH, killed pathogenic bacteria and allowed lactic acid bacteria to survive at pH 5.5. Conclusion: The formulation ratio obtained through this study could be used for the development of a feminine cleanser that can be used as a substitute for antibacterial agents. Further, the findings of this study may be able to solve the problem of antimicrobial resistance in the future.

A New Composition of Nanosized Silica-Silver for Control of Various Plant Diseases

  • Park Hae-Jun;Kim Sung-Ho;Kim Hwa-Jung;Choi Seong-Ho
    • The Plant Pathology Journal
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    • v.22 no.3
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    • pp.295-302
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    • 2006
  • The present study addressed the efficacy of nanosized silica-silver for controlling plant pathogenic microorganisms. The nanosized silica-silver consisted of nano-silver combined with silica molecules and water soluble polymer, prepared by exposing a solution including silver salt, silicate and water soluble polymer to radioactive rays. The nanosized silica-silver showed antifungal activity against the tested phytopathogenic fungi at 3.0 ppm with varied degrees. In contrast, a number of beneficial bacteria or plant pathogenic bacteria were not significantly affected at 10 ppm level but completely inhibited by 100 ppm of nanosized silicasilver. Among the tested plant pathogenic fungi, the new product effectively controlled powdery mildews of pumpkin at 0.3 ppm in both field and greenhouse tests. The pathogens disappeared from the infected leaves 3 days after spray and the plants remained healthy thereafter. Our results suggested that the product developed in this study was effective in controlling various plant fungal diseases.

Antibacterial Activities of B. polyfermenticus SCD Against Pathogenic Bacteria and Effects on Animals and Humans (Bacillus polyfermenticus SCD의 병원성 세균에 대한 항균성과 동물 및 임상에 미치는 영향)

  • 강재선;전경동;김원석;조우성;권주열;문경호
    • YAKHAK HOEJI
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    • v.48 no.1
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    • pp.70-74
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    • 2004
  • Bacillus polyfermenticus SCD which is commonly called as Bisroot (equation omitted) has been appropriately used for the treatment of long-term intestinal disorder's. This strain strongly inhibited against methicillin resistance Staphylococcus aureus (MRSA) and various pathogenic microorganisms. Effects of B. polyfermentius SCD administration on death rates and egg-laying rates in two groups of hens were investigated. This strain decreased the death rates of two groups by 16.26% and 11.72%, respectively. Also this strain increased the egg-laying rates of those 2.74% and 2.66%, respectively Clinical tests of B. polyfermenticus SCD administration to healthy adults showed not adverse effects but decreased glyceride concentration from 154.52 mg/dl to 135.41 mg/dl after two week administrations.

Empirical Modeling of Fouling Rate of Milk Pasteurization Process : A case study

  • Budiati, Titik;Wahyono, Nanang Dwi;Hefni, Muh.
    • International journal of advanced smart convergence
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    • v.4 no.1
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    • pp.11-17
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    • 2015
  • Fouling in heat exchanger becomes a major problem of dairy industry and it increases the production cost. These are lost productivity, additional energy, additional equipment, chemical, manpower, and environmental impact. Fouling also introduces the risk of food safety due to the improper heating temperature which allow the survival of pathogenic bacteria in milk, introducing biofilm formation of pathogenic bacteria in equipments and spreading the pathogenic bacteria to milk. The aim of this study is to determine the fouling rate during pasteurization process in heat exchanger of pasteurized milk produced by Village Cooperative Society (KUD) "X" in Malang, East Java Indonesia by using empirical modeling. The fouling rate is found as $0.3945^{\circ}C/h$ with the heating process time ranged from 0 to 2 hours and temperature difference (hot water inlet temperature and milk outlet temperature) ranged from 0.654 to $1.636^{\circ}C$. The fouling rate depends on type and characteristics of heat exchangers, time and temperature of process, milk type, age of milk, seasonal variations, the presence of microorganism and more. This results will be used to plan Cleaning In Place (CIP) and to design the control system of pasteurization process in order to maintain the milk outlet temperature as standard of pasteurization.

Pharmacodynamics of florfenicol alone and in combination with amoxicillin or cefuroxime against pathogenic bacteria of fish origin (Florfenicol-amoxicillin 혹은 florfenicol-cefuroxime 병용투여시 어류 병원성 세균에 대한 약역학)

  • Lee, Eun-Mi;Choi, Myung-Jin;Lee, Seung-Jin;Park, Seung-Chun
    • Korean Journal of Veterinary Research
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    • v.50 no.4
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    • pp.279-284
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    • 2010
  • Combination therapy of antibiotics is leading to improved efficacy or safety profiles with decrease emergence of bacterial resistance. Because of this benefit, many of antibacterial combinations have been used in veterinary practice for the past few decades. The purpose of this study was to examine the in vitro activity of an amoxicillin alone and in combination with other antibiotics against pathogenic bacteria of fish origin. Based on the fractional inhibitory concentration (FIC) index (FIC $$\leq_-$$ 0.5), a synergistic interaction was shown in combination of florfenicol with amoxicillin or cefuroxime. The combination of florfenicol and amoxillin showed higher antibacterial activity than that of florfenicol and cefuroxime. Ratio of amoxicillin and florfenicol in combination was 1 : 1, which showed the antibacterial activity against bacterial isolates of fish as compared with other ratios. A synergetic effect of the combination (amoxicillin and florfenicol) was further confirmed in the time-kill curve study. The study showed a better in vitro antibacterial activity of a 1 : 1 combination of amoxicillin and florfenicol than the individual antibacterial against bacterial isolates of fish. In conclusion, the combination of florfenicol and amoxicillin may serve as a potential antibacterial therapy in fishes infected pathogenic bacteria.

Antimicrobial Activities of Alkyl Gallates Alone and in Combination with Antibiotics Against the Fish Pathogenic Bacteria Edwardsiella tarda and Vibrio anguillarum (알킬갈레이트 유도체의 어병세균 Edwardsiella tarda와 Vibrio anguillarum에 대한 항균활성 및 항생제와의 병용효과)

  • Kang, So-Young;Kang, Ji-Young;Kim, Su-Yeon;Kim, Do-Hyung;Oh, Myung-Joo
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.41 no.3
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    • pp.188-192
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    • 2008
  • Methyl gallate isolated from bark of the tree Rhus verniciflua Stokes has significant antimicrobial activity against the fish pathogenic bacteria Edwardsiella tarda and Vibrio anguillarum. To evaluate the antimicrobial activity of gallate derivatives, eight alkyl gallates were tested. Ethyl gallate and propyl gallate had the highest activities, with MICs of $15.6-31.3{\mu}g/mL$ against E. tarda. For V. anguillarum, propyl gallate and butyl gallate were highly effective, with MICs of $7.81-31.3{\mu}g/mL$. When used in combination with antibiotics, methyl gallate exhibited synergistic effects with oxytetracycline against E. tarda and with norfloxacin against V. anguillarum. These results suggest that short-chain alkyl gallates can be used as alternatives to antibiotics against the fish pathogenic bacteria.

Studies on the Plant Plant Pathogenic Corynebacteria; The Synthesis of B Group Vitamins by Plant Pathogenic Bacteria (Corynebacterium 속 식물병원세균에 관한 연구 -식물병원세균의 Vitamin B군의 합성 -)

  • Kim Jong-wan;Mukoo Hideo
    • Korean journal of applied entomology
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    • v.14 no.3 s.24
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    • pp.155-161
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    • 1975
  • The results of studies on the synthesis of B group vitamins by plant pathogenic bacteria indicate that most bacteria utilize thiamine, nicotinic acid, biotin and P-Aminobenzoic acid as growth factors. Riboflavin (vitamin $B_2$) was produced by most bacterial genera including the Corynebacteria but with the exception of C. rathay and C.fasciant. The results suggest that the ability to produce riboflavin is not a generic characteristic of Corynebacterium, and that the accuracy of the ultra-violet light method (one of the diagnostic tests for potato bacterial ring rot disease caused by Corynebacterium sepedonicum) must he reconsidered.

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Comparison on Inactivation of Enterobacter sakazakii, Salmonella typhimurium, and Bacillus cereus Inoculated on Infant Formula During Storage by Gamma Irradiation

  • Jin, You-Young;Ku, Kyung-Ju;Park, Ji-Yong;Park, Jong-Hyun;Chung, Myong-Soo;Kwon, Ki-Sung;Chung, Kyung-Sook;Won, Mi-Sun;Song, Kyung-Bin
    • Food Science and Biotechnology
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    • v.17 no.4
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    • pp.861-864
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    • 2008
  • Enterobacter sakazakii, Salmonella typhimurium. and Bacillus cereus were evaluated on inoculated infant formula by gamma irradiation treatment as a method to provide microbial safety. The infant formula inoculated with the major pathogenic bacteria was treated at irradiation dose of 0, 3, 5, and 10 kGy, respectively. After treatment, the samples were individually packaged and stored at $20^{\circ}C$. Microbiological data during storage represented that the populations of E. sakazakii, S. typhimurium, and B. cereus were reduced with the increase of irradiation dose by 4 to 5 log reductions. In particular, E. sakazakii, S. typhimurium. and B. cereus were eliminated at 10, 5, and 3 kGy, respectively. E. sakazakii was the most radiation-resistant, while B. cereus was the least. Our results represent that gamma irradiation below 10 kGy should eliminate the growth of the major pathogenic bacteria in infant formula during storage.

In vitro antimicrobial activity of Korean propolis against fish pathogenic bacteria (Propolis의 어류 병원성 세균에 대한 in vitro 항균 효과)

  • Heo, Gang-Joon;Won, Tae-Gyeong;Shin, Gee-Wook
    • Korean Journal of Veterinary Service
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    • v.38 no.2
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    • pp.65-70
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    • 2015
  • The present study was to investigate in vitro antimicrobial effects of propolis against six different fish bacterial pathogens, Aeromonas hydrophila, Edwardsiella tarda, Vibrio vulnificus, V. parahaemolyticus, A. salmonicida subsp. masoucida, A. salmonicida subsp. salmonicida) using minimum inhibitory concentration (MIC) and minimum bacteriocidal concentration (MBC) tests. In the results, propolis exhibited antimicrobial activity against all bacteria used in the present study, but there was no marked difference in bacterial species except Vibrio species. Collectively, propolis was thought to be an usefulness antimicrobial substance for controlling bacterial diseases in the fish industry.

A study on the effect of sterilization of the thermometer c three disinfectant sponges (구강체온계의 각 소독솜 별 소독효과에 관한 실험연구)

  • 강수금
    • Journal of Korean Academy of Nursing
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    • v.10 no.2
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    • pp.13-20
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    • 1980
  • This present study was undertaken to assess the effect of sterilization of the thermometer with three sorts of disinfectant sponges-0.1 % bichloride of mercury sponge 70% alcohol sponge, 0.5% zephiran chloride sponge,- by bacterial culture methods in 10 admitted adult patients in S. Hospital, Mok Po city. The results obtained were as follows ; 1. The thermometer sterilized with 0.1 % bichloride of mercury sponge showed no growth of bacteria organism in 5 cases but showed growth of pathogenic organism in 1 case and non pathogenic organism in 4 cases. 2. The thermomerer sterilized with 70 % alcohol sponge showed no growth of bacteria organism in 8 cases but showed growth of nonpathogenic organism in 2 cases. 3. The themometer sterilized with 0.5 % zephiran chloride sponge showed no growth of bacteria organism in all 10 cases. From these results it could be concluded that 0.5 % zephiran chloride would be most effective in sterilization of thermometer.

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