• International Journal of Oral Biology
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• 제39권4호
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• pp.229-236
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• 2014

Reactive oxygen species (ROS) and nitrogen species (RNS) are implicated in cellular signaling processes and as a cause of oxidative stress. Recent studies indicate that ROS and RNS are important signaling molecules involved in nociceptive transmission. Xanthine oxidase (XO) system is a well-known system for superoxide anions ($O{_2}^{{\cdot}_-}$) generation, and sodium nitroprusside (SNP) is a representative nitric oxide (NO) donor. Patch clamp recording in spinal slices was used to investigate the role of $O{_2}^{{\cdot}_-}$ and NO on substantia gelatinosa (SG) neuronal excitability. Application of xanthine and xanthine oxidase (X/XO) compound induced membrane depolarization. Low concentration SNP ($10{\mu}M$) induced depolarization of the membrane, whereas high concentration SNP (1 mM) evoked membrane hyperpolarization. These responses were significantly decreased by pretreatment with phenyl N-tert-butylnitrone (PBN; nonspecific ROS and RNS scavenger). Addition of thapsigargin to an external calcium free solution for blocking synaptic transmission, led to significantly decreased X/XO-induced responses. Additionally, X/XO and SNP-induced responses were unchanged in the presence of intracellular applied PBN, indicative of the involvement of presynaptic action. Inclusion of GDP-${\beta}$-S or suramin (G protein inhibitors) in the patch pipette decreased SNP-induced responses, whereas it failed to decrease X/XO-induced responses. Pretreatment with n-ethylmaleimide (NEM; thiol-alkylating agent) decreased the effects of SNP, suggesting that these responses were mediated by direct oxidation of channel protein, whereas X/XO-induced responses were unchanged. These data suggested that ROS and RNS play distinct roles in the regulation of the membrane excitability of SG neurons related to the pain transmission.

• 한국정보통신학회논문지
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• 제27권1호
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• pp.8-14
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• 2023

기존의 자기지도 학습 기반의 CutPaste 기법은 정상 이미지에서 특정 패치를 자르고 붙이는 방법으로 합성 데이터를 생성한 뒤 이상탐지를 수행하였다. 그러나 이런 방식으로 생성된 합성데이터는 패치의 경계에 뚜렷한 차이가 나타나는 문제가 발생된다. 이러한 문제를 해결하기 위한 NSA 기법은 Poisson Blending을 통해 자연스러운 합성 데이터를 생성하여 더 높은 이상탐지 성능을 달성하였다. 그러나 NSA 기법은 클래스마다 조정해야하는 하이퍼 파라미터가 많은 단점을 가지고 있다. 본 논문에서는 합성 패치의 크기를 매우 작게 하는 단순한 방법으로 정상과 유사한 합성 데이터를 생성하였다. 이 때 패치가 매우 지역적으로 합성되기 때문에, 지역적인 특징을 학습하는 모델을 사용하면 합성 데이터에 쉽게 과적합 될 수 있다. 따라서 전역적인 특징을 학습하는 gMLP를 사용하여 이상탐지를 수행하였고, 단순한 합성 방법으로도 기존 자기 지도 학습 기법보다 더 높은 성능을 달성할 수 있었다.

• Molecules and Cells
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• 제27권3호
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• pp.307-312
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• 2009

The interstitial cells of Cajal (ICC) are pacemaking cells required for gastrointestinal motility. The possibility of whether DA-9701, a novel prokinetic agent formulated with Pharbitis Semen and Corydalis Tuber, modulates pacemaker activities in the ICC was tested using the whole cell patch clamp technique. DA-9701 produced membrane depolarization and increased tonic inward pacemaker currents in the voltage-clamp mode. The application of flufenamic acid, a non-selective cation channel blocker, but not niflumic acid, abolished the generation of pacemaker currents induced by DA-9701. Pretreatment with a $Ca^{2+}$-free solution and thapsigargin, a $Ca^{2+}$-ATPase inhibitor in the endoplasmic reticulum, abolished the generation of pacemaker currents. In addition, the tonic inward currents were inhibited by U-73122, an active phospholipase C inhibitor, but not by $GDP-{\beta}-S$, which permanently binds G-binding proteins. Furthermore, the protein kinase C inhibitors, chelerythrine and calphostin C, did not block the DA-9701-induced pacemaker currents. These results suggest that DA-9701 might affect gastrointestinal motility by the modulation of pacemaker activity in the ICC, and the activation is associated with the non-selective cationic channels via external $Ca^{2+}$ influx, phospholipase C activation, and $Ca^{2+}$ release from internal storage in a G protein-independent and protein kinase C-independent manner.

• The Korean Journal of Physiology and Pharmacology
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• 제18권4호
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• pp.341-346
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• 2014

Lubiprostone is a chloride ($Cl^-$) channel activator derived from prostaglandin $E_1$ and used for managing constipation. In addition, lubiprostone affects the activity of gastrointestinal smooth muscles. Interstitial cells of Cajal (ICCs) are pacemaker cells that generate slow-wave activity in smooth muscles. We studied the effects of lubiprostone on the pacemaker potentials of colonic ICCs. We used the whole-cell patch-clamp technique to determine the pacemaker activity in cultured colonic ICCs obtained from mice. Lubiprostone hyperpolarized the membrane and inhibited the generation of pacemaker potentials. Prostanoid $EP_1$, $EP_2$, $EP_3$, and $EP_4$ antagonists (SC-19220, PF-04418948, 6-methoxypyridine-2-boronc acid N-phenyldiethanolamine ester, and GW627368, respectively) did not block the response to lubiprostone. L-NG-nitroarginine methyl ester (L-NAME, an inhibitor of nitric oxide synthase) and 1H-[1,2,4]oxadiazolo[4,3,-a]quinoxalin-1-one (ODQ, an inhibitor of guanylate cyclase) did not block the response to lubiprostone. In addition, tetraethylammonium (TEA, a voltage-dependent potassium [$K^+$] channel blocker) and apamin (a calcium [$Ca^{2+}$]-dependent $K^+$ channel blocker) did not block the response to lubiprostone. However, glibenclamide (an ATP-sensitive $K^+$ channel blocker) blocked the response to lubiprostone. Similar to lubiprostone, pinacidil (an opener of ATP-sensitive $K^+$ channel) hyperpolarized the membrane and inhibited the generation of pacemaker potentials, and these effects were inhibited by glibenclamide. These results suggest that lubiprostone can modulate the pacemaker potentials of colonic ICCs via activation of ATP-sensitive $K^+$ channel through a prostanoid EP receptor-independent mechanism.

• 한국지리정보학회지
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• 제13권1호
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• pp.114-127
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• 2010

본 연구의 목적은 경상남도 창원시를 사례지역으로 기후생태적 기능평가요인을 활용하여 차고 신선한 공기 생성지역을 규명하는 것이다. 기후생태적 기능평가는 찬공기 생성 및 유입기능지역의 면적, 농지 및 초지비율, 평균경사도, 횡단면유형, 평균경사길이, 계곡기저부 거칠기의 6개 요인들로 이루어졌다. 연구대상지의 평가요인별 분석결과는 찬공기 생성능력에 따라 5개 등급으로 구분하였다. 찬공기 생성기능이 가장 높은 1등급 지역은 전체 연구대상지의 3.51%, 2등급 지역 13.48%, 3등급 지역 31.65%, 4등급 지역 27.28%, 5등급 지역 24.09%를 각각 차지하였다. 찬공기 생성지역의 공간적 분포에 따르면, 정병산과 창원터널, 안민터널 일대 도시외곽 산지의 계곡부 지역들이 높은 평가등급을 가지는 것으로 나타났다. 향후 이러한 도시외곽의 찬공기 생성지역에 대한 장기적인 미기상 모니터링을 토대로 기후생태적 보전지역의 설정과 바람통로 조성 등의 연구가 필요할 것으로 판단된다.

• 대한원격탐사학회지
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• 제33권1호
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• pp.97-109
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• 2017

Green tides that developed along the coast of China in 2015 were detected and tracked using vegetation indices from GOCI and Landsat images. Green tides first appeared near the Jiangsu Province on May 14 before increasing in size and number and moving northward to the Shandong Peninsula in mid-June. Typhoon Cham-hom passed through the Yellow Sea on July 12, significantly decreasing the algal population. An algae patch moved east toward Korea and on June 18 and July 4, several masses were found between the southwestern shores of Korea and Jeju Island. The floating masses found in Korean waters were concentrated at the boundary of the open sea and the Jindo cold pool, a phenomenon also observed at the boundary of coastal and offshore waters in China. Sea surface temperatures, derived from NOAA SST data, were found to play a role in generation of the green tides.

• 한국전산구조공학회:학술대회논문집
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• 한국전산구조공학회 2003년도 봄 학술발표회 논문집
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• pp.288-295
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• 2003

An adaptive procedure in finite element analysis is presented by p-refinement of meshes in conjunction with a posteriori error estimator that is based on the recovery technique. In case of the recovery technique, the SPR(superconvergent patch recovery) approach has been modified for p-adaptive mesh refinement. The strategy of finding a nearly optimal distribution of polynomial degrees on a fixed finite element mesh is discussed such that a particular element has to be refined automatically to obtain an acceptable level of accuracy by increasing p-levels non-uniformly. To verify the proposed algorithm, the limit value approach is proposed which utilizes the exact strain energy computed from the extrapolation equation. A new pre-processor is developed for the p-version finite element program in which the vector graphic editor is used for the automatic generation of node connection and coordinate by halfedge solid data structure according to uniform or nonuniform p-distribution. The general 2-D algorithm is also developed to generate face modes and internal modes in accordance with different mesh types. The quality of the error estimator is investigated with the help of two mumerical examples. The results show that the sequences of p-distributions obtained by the proposed error indicator closely follow the optimal trajectory.

• The Korean Journal of Physiology and Pharmacology
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• 제1권4호
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• pp.355-366
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• 1997

Although cerebellar Purkinje cells display spontaneous electrical activity in vivo and in slice experiments, the mechanism of the spontaneous activity generation has not been clearly understood. The aim of this study was to investigate whether cerebellar Purkinje cells of postnatal rats generate spontaneous electrical activity without synaptic inputs. Dissociated cerebellar Purkinje cells were used for reducing synaptic inputs in the present study. Cerebellar Purkinje cells with dendrites were dissociated from postnatal rats using enzymatic treatment followed by mechanical trituration. Spontaneous electrical activities were recorded from dissociated cells without any stimulus using whole-cell patch clamp configuration. Two types, spontaneously firing or quiescent, of dissociated Purkinje cells were observed in postnatal rats. Both types of cells were identified as Purkinje cells using immunocytochemical staining technique with anti-calbindin after recording. Spontaneously active cells displayed two patterns of firing, repetitive and burst firings. Two thirds of dissociated Purkinje cells displayed repetitive firing and the rest of them did burst firing under same recording condition. Repetitive firing activities were maintained even after further isolation using either physical or pharmacological techniques. Neither high magnessium solution nor excitatory synaptic blockers, AP-5 and DNQX, block the spontaneous activity. These results demonstrate that spontaneous electrical activity of isolated cerebellar Purkinje cells in postnatal rats is generated by intrinsic membrane properties rather than synaptic inputs.

• The Korean Journal of Physiology and Pharmacology
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• 제25권3호
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• pp.251-258
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• 2021

Flos magnoliae (FM), the dry flower buds of Magnolia officinalis or its related species, is a traditional herbal medicine commonly used in Asia for symptomatic relief of and treating allergic rhinitis, headache, and sinusitis. Although several studies have reported the effects of FM on store-operated calcium entry (SOCE) via the ORAI1 channel, which is essential during intracellular calcium signaling cascade generation for T cell activation and mast cell degranulation, the effects of its isolated constituents on SOCE remain unidentified. Therefore, we investigated which of the five major constituents of 30% ethanoic FM (vanillic acid, tiliroside, eudesmin, magnolin, and fargesin) inhibit SOCE and their physiological effects on immune cells. The conventional whole-cell patch clamp results showed that fargesin, magnolin, and eudesmin significantly inhibited SOCE and thus human primary CD4+ T lymphocyte proliferation, as well as allergen-induced histamine release in mast cells. Among them, fargesin demonstrated the most potent inhibitory effects not only on ORAI1 (IC50 = 12.46 ± 1.300 μM) but also on T-cell proliferation (by 87.74% ± 1.835%) and mast cell degranulation (by 20.11% ± 5.366%) at 100 μM. Our findings suggest that fargesin can be a promising candidate for the development of therapeutic drugs to treat allergic diseases.

• Journal of Genetic Medicine
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• 제18권2호
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• pp.147-151
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• 2021

The Say-Barber-Biesecker-Young-Simpson variant of Ohdo syndrome (SBBYSS) (Online Mendelian Inheritance in Man #603736) is a rare autosomal dominant disorder and clinically features blepharophimosis with ptosis, a mask-like facial appearance, cryptorchidism, congenital heart defect, long thumbs/great toes, and thyroid dysfunction. The etiology of SBBYSS has been shown to be due to heterozygous KAT6B gene mutation. Here we report a case of a neonate with SBBYSS identified a novel mutation in KAT6B gene. The patient showed typical dysmorphic facies, cryptorchidism with micropenis, overriding fingers, and long thumbs and toes at birth. He had also hypothyroidism, large atrial septal defect, and sensorineural hearing loss. The next generation sequencing identified a heterozygous novel variant, c.5206C>T (p.Gln1736Ter) in KAT6B gene. At the 9 months of age, he underwent patch closure for atrial septal defect. Until the 12-month follow-up, he was under-developed.