• 대한소아치과학회지
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• 제43권4호
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• pp.419-426
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• 2016

본 연구는 상악 매복 과잉치를 가지고 전신질환 및 의과 병력이 없는 만 5 - 9세 사이의 남녀 20명으로부터 서면동의를 얻고 상악 전치부에 매복된 과잉치를 발치하고 치수세포를 채취하였다. 채취 후 세포의 1차 배양 동안 오염된 3명(남자 2명, 여자 1명)을 제외하고 총 17명(남자 10명, 여자 7명)을 대상으로 하였다. 이번 연구에서 과잉치 치수조직으로부터 유래한 세포의 계대 배양에 소요된 평균 시간은 $2.91{\pm}0.29$일이었다. 최초 치수조직으로부터 세포를 얻은 후 80% confluency를 얻는데 소요되는 시간이 $4.53{\pm}0.94$일로 가장 많이 걸렸고, 이후 계대부터는 $2.73{\pm}0.32$일이 소요되었다. 남자의 비율은 58.82%, 여자의 비율은 41.18%이었다. 여자의 평균 소요시간은 $2.81{\pm}0.27$일 이었으며, 남자는 $2.98{\pm}0.29$이었다. 과잉치가 역위된 비율은 82.35%, 정위 비율은 17.65%였다. 평균 계대 소요 배양 시간은 역위가 $2.94{\pm}0.30$일, 정위는 $2.80{\pm}0.20$일이었다. 원뿔형(Conical type)은 76.47%였고, 그 외 형태는 23.53%였다. 원뿔형의 평균 소요시간은 $2.92{\pm}0.31$이었고, 그 외 형태의 소요 시간은 $2.88{\pm}0.22$이었다. 매복 방향과 형태에 따른 계대 배향 시간은 통계적으로 유의한 차이를 보이지 않았다. 향후 초기 계대와 후기 계대에서 얻어진 세포의 줄기세포로써의 능력을 평가하는 후속 연구들이 필요하며, 3일 이내의 계대 배양 시간이 소요된 점을 고려할 때, 연구의 효율성과 빠른 배양시간 등은 과잉치 유래세포가 치아유래 줄기세포의 연구에 활용가능성이 충분하리라 판단되었다.

• Asian Pacific Journal of Cancer Prevention
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• 제15권12호
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• pp.4871-4876
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• 2014

Background: We sought to evaluate the role of tumor associated macrophages (TAMs) on the promotion of coal tar pitch extract (CTPE)-induced tumorigenesis of human bronchial epithelial cells (BEAS-2B) and tumor metastasis in nude mice, and related mechanisms. Materials and Methods: BEAS-2B cells were first treated with 2.4 mg/mL CTPE for 72 hours. After removal of CTPE, the cells were continuously cultured and passaged using trypsin-EDTA. THP-1 cells were used as macrophage-like cells. BEAS-2B cells under different conditions (n=6/group) were injected into the back necks of nude mice, and alterations of tumor xenograft growth, indicative of tumorigenicity, and tumor metastasis were determined. Pathological changes (tumor nests and microvascular lesions) of HE-stained tumor tissues were also evaluated. The expression of AP-1(c-Jun) in xenografts and metastatic tumors was determined using immunohistochemistry. Results: Tumor size and weight in nude mice transplanted with the mixture of CTPE-induced passage 30 BEAS-2B and THP-1 cells (2:1) were increased compared to those from the CTPE-treated BEAS-2B cells at passage 30 alone at different observation time points. Tumor metastasis to lymph nodes and liver was only detected after transplantation of a mixture the two kinds of cells. The numbers of tumor nests and microvascular lesions, and the expression levels of AP-1 (c-Jun) in tumors from the mixture of two kinds of cells were increased apparently in contrast to those in tumor from the CTPE-treated BEAS-2B cells of passage 30 alone. In addition, there was positive correlation between AP-1 (c-Jun) expression level and the number of microvascular lesions, or between AP-1 (c-Jun) expression level and tumor metastasis in these two groups. Conclusions: TAMs not only facilitate tumorigenesis transformation of CTPE-induced BEAS-2B cells, but also promote tumor growth, angiogenesis and metastasis in nude mice in vivo, which may be mediated by AP-1.

• 한국철도학회논문집
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• 제12권1호
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• pp.9-15
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• 2009

본 연구는 축소실험을 통하여 터널내 화재발생시 구난역 내 설계된 환기 시스템 성능을 검증 하였다. Froude 수 상사법칙을 적용하여 실제 터널 크기의 1/35로 축소된 모형터널을 사용하였다. 모형터널은 두께가 2mm인 철을 사용하여 총 길이 10m, 높이 0.19m, 폭 0.26m으로 제작되었다. Cross-passage는 사고터널과 구난 터널 사이에 연결되고 Cross-passage 중앙에는 방화문이 설치되어 있다. 실험에는 n-heptane, $4cm{\times}4cm$ 풀화재를 이용하였다. 화원의 발열량은 695.97W이고, 화원 위치는 터널 중앙과 가장 위험한 경우로써 터널 입구지점에 각각 설치하였다. 환기조건은 0.015cms이고 화원과 가장 인접한 곳에서만 배기하도록 하였다. 구난역 배기 성능을 검증하기 위해 온도와 CO농도를 측정하여 연기의 유무를 파악하였다. 본 실험의 결과는 다음과 같다. 첫째, 터널 중앙화원인 경우 환기가 없어도 구난 터널에 연기가 검출되지 않았다. 둘째, 구난역 입구 부근에 화원을 설치한 경우 환기 조건을 주지 않으면 연기가 구난 터널로 침투하지만, 설계된 배기 조건시 연기는 구난터널로 전파하지 않았다.

• 한국수정란이식학회지
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• 제28권4호
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• pp.373-379
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• 2013

Spermatogonial stem cells (SSCs) developed into sperms through spermatogenesis have been utilized as a useful tool in the field of regenerative medicine and infertility. However, a small number of highly qualified SSCs are resided in the seminiferous tubule of testis, resulted in developing effective in-vitro culture system of SSCs for solving simultaneously quantitative and qualitative problems. Presently, SSCs can be enriched on testicular stromal cells (TSCs), but there are no systematic researches about TSC culture. Therefore, we tried to optimize culture condition of TSCs derived from mouse with different strains. For these, proliferation and viability were measured and compared by culturing ICR outbred or DBA/2 inbred mouse-derived TSCs at 35 or $37^{\circ}C$. In case of ICR strain, primary TSCs cultured at $37^{\circ}C$ showed significantly higher proliferation and viability than those at $35^{\circ}C$ and significant increase of proliferation and viability in sub-passaged TSCs was detected in the $35^{\circ}C$ culture condition. Moreover, sub-passage of primary TSCs at $35^{\circ}C$ induced no significant effects on proliferation and viability. In contrast, in case of DBA/2 strain, significantly improved proliferation were detected in the primary TSCs cultured at $35^{\circ}C$, which showed no significant difference in the viability, compared to those at $37^{\circ}C$. Furthermore, sub-passaged TSCs cultured at $37^{\circ}C$ showed no significant differences in proliferation and viability, compared to those at $35^{\circ}C$. However, with significant decrease of proliferation induced by sub-passage of primary TSCs at $35^{\circ}C$, no significant effects on proliferation and viability were resulted from sub-passage of primary TSCs at $37^{\circ}C$. From these results, culture temperature of primary TSCs derived from outbred and inbred strain of mouse could be separately optimized in primary culture and subculture.

• 한국도시철도학회논문집
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• 제6권4호
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• pp.249-257
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• 2018

교통사상사고 원인 중 선로근접 및 무단통행으로 인한 사고가 많이 발생하고 있다. 운행선 근접공사 시 열차운행에 지장을 주지 않고, 작업자의 안전확보 및 작업효율을 높이기 위해 철도선로용 안전펜스가 사용되고 있다. 하지만 철도선로에 적용되는 레일저부형 안전펜스는 열차주행 시 안전펜스의 간섭여부를 확인할 필요가 있다. 본 연구에서는 철도선로용 안전펜스의 열차운행 시 지장여부를 확인하기 위하여 국내에서 운행되고 있는 열차들과 안전펜스 설치 시 이격간격을 분석하였다. 또한, 국내 열차종류별 열차 풍압에 대한 횡방향 하중조건을 산정하여 철도선로용 안전펜스의 설치간격에 따른 수치해석을 수행하였다. 본 연구를 통하여 철도선로용 안전펜스의 적정 이격거리는 차량한계로부터 200mm 이상을 확보하고, 열차풍압을 고려한 철도선로용 안전펜스의 적정 설치간격을 산정할 필요가 있음을 확인하였다.

• Journal of Advanced Marine Engineering and Technology
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• 제33권2호
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• pp.244-251
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• 2009

Porous media have especially large surface area per volume, which contain complex fluid passage. If porous media can be applied to cool a CPU or an electronic device with large heat dissipation, it could result in heat transfer enhancement due to the enlargement of the heat transfer area and the flow disturbance. This study is aimed to identify the heat transfer and pressure drop characteristics of high-porosity metal foams in a horizontal channel. Experiment is performed with the various heat flux, velocity and pore density conditions. Permeabilities, which is deduced from Non-Darcy flow model, become lower with increasing pore density. Nusselt number also decreases with higher pore density. High pore density with same porosity case shows higher pressure loss due to the increase of surface area per unit volume. The fiction factor decreases rapidly with increase of Reynolds number in Darcy flow region. However, it converges to a constant value of the Ergun coefficient in Non-Darcy flow region.

• 대한기계학회논문집B
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• 제27권1호
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• pp.54-60
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• 2003

Compound choking frequently occurs at a minimum area of the flow passage, where two or more streams which have different stagnation properties are merged. This phenomenon is especially important in that the flow choking may not be given by Mach number, M=1 at the nozzle throat. In order to obtain a detailed understanding of the flow characteristics involved in the compound flow choking, the two-dimensional, compressible, Wavier-Stokes equations are solved using a fully implicit finite volume method and the predicted solutions are compared with the results of the one-dimensional theoretical analysis. Stagnation pressure and temperature of each stream are changed to investigate the effects on the compound choking. The results show that stagnation pressures of each stream affect Mach number and static pressure distributions downstream of the exit of the convergent nozzle. However, the flow characteristics of the compound choking are not significantly dependent on the total temperature ratio.

• 대한기계학회:학술대회논문집
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• 대한기계학회 2004년도 추계학술대회
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• pp.1104-1109
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• 2004

The present study has been conducted to investigate convective heat/mass transfer inside the cooling passage with bleed holes. The rotating square channel has 40.0 mm hydraulic diameter and the bleed holes on the leading surface of the channel. The hole diameter of bleed hole is 4.5 mm and its spacing (P/d=4.9) is about five times of hole diameter. Mass flow rate through bleed holes is 10% of the main flow rate and rotation number is changed form 0.0 to 0.4. A naphthalene sublimation technique is employed to determine the detailed local heat transfer coefficients using the heat and mass transfer analogy. The cooling performance is influenced by mass flow rate through bleed holes and Coriolis force of rotating channel for fixed reynolds number. The heat transfer is enhanced around holes on the leading surface because of trapping flow by bleeding. However heat transfer on the leading surface is decreased due to Coriolis force.

• 한국응용과학기술학회지
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• 제22권3호
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• pp.241-249
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• 2005

This experiment extracted the natural green-tea using ethanol and obtained the refined oil component after filterated green-tea extract. This study tested the antimicrobial effect as characteristics experiment, and analyzed refined oil component with pH-meter and GC/MS. In the result of this experiment, it obtained the next conclusions. In the first result of extraction experiment, it could know that extraction ratio of refined oil component appeared about 9.0%. In the second result of characteristics experiment, it could certificate that in case of increasing the refined oil component in concentration of 100ppm and above, and according to passage of cultivation time, the number of S-aureus and E-coli in microbe decreased less and less. But in case of blank test not adding the refined oil component, the number of microbe increased more and more. In these phenomena, it could certificate that refined oil component of green-tea appeared antimicrobial effect against microbe. In the third result of instrumental analysis, refined oil component of green-tea appeared about 7.6 in 1% distilled water solution with pH-meter, and the aromatic components of benzene, bonyl acetate, campene, ${\alpha},{\beta},{\gamma}$-pinnene etcs from refined oil component of green-tea was detected with GC/MS.

• 한국안전학회지
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• 제33권5호
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• pp.78-83
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• 2018

Recently, due to the rapid industrialization and urbanization, a great number of infrastructure and population were concentrated in urban areas. These changes have resulted in unprecedent runoff characteristics in urban basins, and the increase in impermeable areas leads to the growth of the runoff and the peak flow rate. Although many cities have made a lot of efforts to check and expand the stormwater network, the flash flood or the local torrential rain caused a growing number of casualty and property damage. This study analyzed the stormwater passage rate in a target area using SWMM. By incorporating the flow quantity surpassing the storm sewer capacity, a 2D inland flooding analysis model was applied to route the inundated area and velocity.