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Sialoglycoproteins of Mammalian Erythrocyte Membranes: A Comparative Study

  • Sharma, Savita;Gokhale, Sadashiv M.
    • Asian-Australasian Journal of Animal Sciences
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    • v.24 no.12
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    • pp.1666-1673
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    • 2011
  • The presence of sialoglycoproteins (SGPs) in the membranes from goat (Capra aegagrus hircus), buffalo (Bubalus bubalis bubalis) and pig (Sus scrofa domestica) erythrocytes was investigated by partial purification with a chloroform-methanol extraction method followed by Sodium dodecyl sulphate - Polyacrylamide gel electrophoresis in comparison to human (Homo sapiens) erythrocytes. The results show that mammalian erythrocytes possess clear differences in the SGPs numbers and molecular weights although all animals studied in this experiment are from the same class i.e. mammalia. The SGPs number in human, goat, buffalo and pig are four (PAS-1 to PAS-4), ten (PAS-GI to PAS-GX), seven (PAS-BI to PAS-BVII) and four (PAS-PI to PAS-IV) respectively as indicated by staining the polyacrylamide gel with sialoglycoprotein-specific Periodic acid-Schiff's (PAS) stain. The new SGPs could be observed only after the partial purification of membrane fractions named as PAS-HI with molecular weight (Mr) 190 kDa and PAS-HII 150 kDa in human, PAS-BIA in buffalo and PAS-PIA and PAS-PIVA in pig. The gels were also stained with Coomassie brilliant blue (CBB) and Silver stain to check the contamination of other membrane proteins in the purified fractions. The quantitative distribution of SGPs was also determined by densitometry. Present study indicates that there are some basic differences in mammalian erythrocyte membrane SGPs, especially with respect to their number and molecular weights indicating major structural variations.

Neighboring Elemental Image Exemplar Based Inpainting for Computational Integral Imaging Reconstruction with Partial Occlusion

  • Ko, Bumseok;Lee, Byung-Gook;Lee, Sukho
    • Journal of the Optical Society of Korea
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    • v.19 no.4
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    • pp.390-396
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    • 2015
  • We propose a partial occlusion removal method for computational integral imaging reconstruction (CIIR) based on the usage of the exemplar based inpainting technique. The proposed method is an improved version of the original linear inpainting based CIIR (LI-CIIR), which uses the inpainting technique to fill in the data missing region. The LI-CIIR shows good results for images which contain objects with smooth surfaces. However, if the object has a textured surface, the result of the LI-CIIR deteriorates, since the linear inpainting cannot recover the textured data in the data missing region well. In this work, we utilize the exemplar based inpainting to fill in the textured data in the data missing region. We call the proposed method the neighboring elemental image exemplar based inpainting (NEI-exemplar inpainting) method, since it uses sources from neighboring elemental images to fill in the data missing region. Furthermore, we also propose an automatic occluding region extraction method based on the use of the mutual constraint using depth estimation (MC-DE) and the level set based bimodal segmentation. Experimental results show the validity of the proposed system.

A Study on Factor Analytical Methods and Procedures for PLS-SEM (Partial Least Squares Structural Equation Modeling)

  • YIM, Myung-Seong
    • The Journal of Industrial Distribution & Business
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    • v.10 no.5
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    • pp.7-20
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    • 2019
  • Purpose - This study provides appropriate procedures for EFA to help researchers conduct empirical studies by using PLS-SEM. Research design, data, and methodology - This study addresses the absolute and relative sample size criteria, sampling adequacy, factor extraction models, factor rotation methods, the criterion for the number of factors to retain, interpretation of results, and reporting information. Results - The factor analysis procedure for PLS-SEM consists of the following five stages. First, it is important to look at whether both the Bartlett test of sphericity and the KMO MSA meet the qualitative criteria. Second, PAF is a better choice of methodology. Third, an oblique technique is a suitable method for PLS-SEM. Fourth, a combined approach is strongly recommended to factor retention. PA should be used at the onset. Next, it is recommended using the K1 criterion. In addition, it is necessary to extract factors that increase the total variance explanatory power through the PVA-FS. Finally, it is appropriate to select an item with a factor loading into 0.5 or higher and a communality of 0.5. Conclusions - It is expected that the accurate factor analysis processed for PLS-SEM as previously presented will help us extract more precise factors of the structural model.

Molecular identification of the vaccine strain from the inactivated bovine viral diarrhea virus (BVDV) vaccines

  • Yang, Dong-Kun;Kim, Ha-Hyun;Cho, Soo-Dong;Choi, Sung-Suk;Kim, Jae-Jo;Song, Jae-Young
    • Korean Journal of Veterinary Service
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    • v.36 no.1
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    • pp.1-6
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    • 2013
  • Since the 1980's, several kinds of inactivated bovine viral diarrhea virus (BVDV) vaccines have been used to immunize domestic animals such as cattle and goat in Korea. Immunogenicity of the BVDV vaccines has been checked by the Korean Veterinary Authority using laboratory animals. In this study, we applied a molecular method to investigate the genetic characterization of the BVDV genes in six commercial inactivated BVDV vaccines, and determined the efficiency of two extraction reagents (i.e., sodium citrate or isopropyl myristate) to separate the vaccine antigens from the antigen/adjuvant complexes. Six partial non-coding regions (288 bp) were successfully amplified with specific primer sets, which demonstrated that sodium citrate is more efficient in extracting viral RNA from inactivated gel vaccines than isopropyl myristae. In addition, we identified the virus strains from the vaccines by analyzing the nucleotide sequences of the 5' non-coding region (NCR) of BVDV. The nucleotide similarity of the partial 5' NCR ranged from 95.1 to 100% among BVDV vaccine strains, respectively, indicating that a few manufacturers used different BVDV strains to produce their vaccines.

Signal Classification of Electromagnetic Wave for Contaminated Degree on Polymer Insulator (고분자 애자 오손정도의 전자파 신호 분석)

  • Park, Jae-Jun;Choi, In-Hyuk;Jung, Myeong-Yeon;Lee, Seung-Wook;Seo, Euy-Won;Ka, Pyeong-Hyeon
    • Proceedings of the Korean Institute of Electrical and Electronic Material Engineers Conference
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    • 2004.11a
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    • pp.662-665
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    • 2004
  • Recently, diagnosis techniques have been investigated to detect a partial discharge associated with a dielectric material defect in a high-voltage electrical apparatus However, the properties of detection technique of PD aren't completely understood because the physical process of PD. Therefore, this paper analyzes the process on Surface Discharge of Polymer Insulator using Wavelet transform. Wavelet transform provides a direct quantitative measure of spectral content in the time-frequency domain. As it is important to develop a non-contact method for detecting the Contamination Degree, this paper analyzes the electromagnetic waves emitted from PD using Wavelet transform. This paper experimentally shows the process of PD as a two-dimensional distribution in the time-frequency domain. This method is shown to be useful for detecting prediction of contamination degree.

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Identification System Based on Partial Face Feature Extraction (부분 얼굴 특징 추출에 기반한 신원 확인 시스템)

  • Choi, Sun-Hyung;Cho, Seong-Won;Chung, Sun-Tae
    • Journal of the Korean Institute of Intelligent Systems
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    • v.22 no.2
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    • pp.168-173
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    • 2012
  • This paper presents a new human identification algorithm using partial features of the uncovered portion of face when a person wears a mask. After the face area is detected, the feature is extracted from the eye area above the mask. The identification process is performed by comparing the acquired one with the registered features. For extracting features SIFT(scale invariant feature transform) algorithm is used. The extracted features are independent of brightness and size- and rotation-invariant for the image. The experiment results show the effectiveness of the suggested algorithm.

Signal analysis of surface discharge and electromagnetic wave for insulator by kaolin contamination (카올린으로 오손된 애자의 표면방전 및 방사전자파의 신호 분석)

  • Park, Jae-Jun
    • The Journal of Information Technology
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    • v.7 no.3
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    • pp.113-118
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    • 2004
  • Recently, diagnosis techniques have been investigated to detect a partial discharge associated with a dielectric material defect in a high voltage electrical apparatus. However, the properties of detection technique of PD aren't completely understood because the physical process of PD. Therefore, this paper analyzes the process on Surface Discharge of Polymer Insulator using Wavelet transform. Wavelet transform provides a direct quantitative measure of spectral content in the time frequency domain. As it is important to develop a non-contact method for detecting the Contamination Degree, this research analyzes the electromagnetic waves emitted from PD using Wavelet transform. This result experimentally shows the process of PD as a two-dimensional distribution in the time-frequency domain. The method is shown to be useful for detecting prediction of contamination degree.

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The Container Pose Measurement Using Computer Vision (컴퓨터 비젼을 이용한 컨테이너 자세 측정)

  • 주기세
    • Journal of the Korea Institute of Information and Communication Engineering
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    • v.8 no.3
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    • pp.702-707
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    • 2004
  • This article is concerned with container pose estimation using CCD a camera and a range sensor. In particular, the issues of characteristic point extraction and image noise reduction are described. The Euler-Lagrange equation for gaussian and random noise reduction is introduced. The alternating direction implicit(ADI) method for solving Euler-Lagrange equation based on partial differential equation(PDE) is applied. The vertex points as characteristic points of a container and a spreader are founded using k order curvature calculation algorithm since the golden and the bisection section algorithm can't solve the local minimum and maximum problems. The proposed algorithm in image preprocess is effective in image denoise. Furthermore, this proposed system using a camera and a range sensor is very low price since the previous system can be used without reconstruction.

Development of an Optimized Feature Extraction Algorithm for Throat Signal Analysis

  • Jung, Young-Giu;Han, Mun-Sung;Lee, Sang-Jo
    • ETRI Journal
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    • v.29 no.3
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    • pp.292-299
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    • 2007
  • In this paper, we present a speech recognition system using a throat microphone. The use of this kind of microphone minimizes the impact of environmental noise. Due to the absence of high frequencies and the partial loss of formant frequencies, previous systems using throat microphones have shown a lower recognition rate than systems which use standard microphones. To develop a high performance automatic speech recognition (ASR) system using only a throat microphone, we propose two methods. First, based on Korean phonological feature theory and a detailed throat signal analysis, we show that it is possible to develop an ASR system using only a throat microphone, and propose conditions of the feature extraction algorithm. Second, we optimize the zero-crossing with peak amplitude (ZCPA) algorithm to guarantee the high performance of the ASR system using only a throat microphone. For ZCPA optimization, we propose an intensification of the formant frequencies and a selection of cochlear filters. Experimental results show that this system yields a performance improvement of about 4% and a reduction in time complexity of 25% when compared to the performance of a standard ZCPA algorithm on throat microphone signals.

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A Study on the Effective Scattering Center Analysis for Radar Cross Section Reduction of Complex Structures (복합구조물의 RCS 저감을 위한 효율적 산란중심 해석에 관한 연구)

  • Kim, Kook-Hyun;Kim, Jin-Hyeong;Cho, Dae-Seung
    • Journal of the Society of Naval Architects of Korea
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    • v.42 no.4 s.142
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    • pp.421-426
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    • 2005
  • Scattering center extraction schemes for radar cross section reduction of large complex targets, like warships, was developed, which are an 1-D radar image method(range profile), and a direct analysis based on an object precision method. The analysis result of partial dihedral model shows that the presented direct analysis method is more efficient than the 1-D radar image method for scattering center extraction of interested targets, in terms of radar cross section reduction design, not signal processing. In order to verify the accuracy of the direct analysis method, a scattering center analysis of an naval weapon system was carried out, and the result was coincident with that of another well-known RCS analysis program. Finally, an analysis result of RCS and its scattering center of an 120m class warship-like model presented that the direct analysis method can be an efficient and powerful tools for radar cross section reduction of large complex targets.