• Journal of the Korean Institute of Telematics and Electronics B
• /
• v.32B no.5
• /
• pp.77-86
• /
• 1995

In this paper in order to reduce the encoding complexity required in the full search vector quantization(VQ), a new classified vector quantization(CVQ) technique is described employing the minimum-distance classifier. The determination of the optimal subcodebook sizes for each class is an important task in CVQ designs and is not an easy work. Therefore letting the subcodebook sizes be equal. A CVQ technique. Which satisties the optimal CVQ condition approximately, is proposed. The proposed CVQ is a kind of the partial search VQ because it requires a search process within each subcodebook only, and the minimum encoding complexity since the subcodebook sizes are the same in each class. But simulation results reveal while the encoding complexity is only O(N$^{1/2}$) comparing with O(N) of the full-search VQ. A simple systolic array, which has the through-put of k, is also proposed for the implementation of the VQ. Since the operation of the classifier is identical with that of the VQ, the proposed array is applied to both the classifier and the VQ in the proposed CVQ, which shows the usefulness of the proposed CVQ.

• BMB Reports
• /
• v.32 no.5
• /
• pp.502-505
• /
• 1999

cDNA fragments of ovine liver pyridoxal kinase were amplified by PCR using degenerate oligonucleotide primers derived from partial amino acids sequences of the enzyme. Using PCR products as probes, several overlapping cDNA clones were isolated independently from an ovine liver and a human brain cDNA library. The largest cDNA clone for each was selected for sequence analysis. The ovine liver cDNA encodes a polypeptide of 297 amino acid residues with Mr of 32,925, whereas the human clone is comprised of an open reading frame encoding 312 amino acid residues with Mr of 35,102. The deduced sequence of the human brain enzyme is completely identical to that of human testes cDNA recently reported (Hanna et al., 1997). The ovine enzymes have approximately 77% sequence identity with the human enzyme although the two sequences are completely different in the N-terminus comprising 32 residues. This result suggests that pyridoxal kinase is highly homologous in mammalian species.

• Journal of the Institute of Electronics Engineers of Korea SD
• /
• v.42 no.2 s.332
• /
• pp.83-88
• /
• 2005

We propose the design method for the VLSI architecture of FNNPDS combined PDS(partial distance search) and FNNS(fast nearest neighbor search), which are used to fast encoding in vector quantization, and obtain the results that FNNPDS(fast nearest neighbor partial distance search) is faster method than the conventional methods by simulation. In simulations, we investigate timing diagrams described searching time of the nearest codevector for an input vector, and compare the average clock cycles per input vector for Lena and Peppers images. According to the result of simulations, the number of the clock cycle of FNNPDS was reduced to $79.2\%\~11.7\%$ as compared with the number using the conventional techniques.

• Journal of fish pathology
• /
• v.33 no.2
• /
• pp.103-109
• /
• 2020

In order to use nervous necrosis virus (NNV) virus-like particles (VLPs) as a delivery tool for heterologous antigens or plasmids, we attempted to produce red-spotted grouper nervous necrosis virus (RGNNV) VLPs displaying a partial region of viral hemorrhagic septicemia virus (VHSV) glycoprotein at the surface and VLPs that are harboring DNA vaccine plasmids within the VLP. A peptide encoding 105 amino acids of VHSV glycoprotein was genetically inserted in the loop region of NNV capsid gene, and VLPs expressing the partial part of VHSV glycoprotein were successfully produced. However, in the transmission electron microscope analysis, the shape and size of the partial VHSV glycoprotein-expressing NNV VLPs were irregular and variable, respectively, indicating that the normal assembly of capsid proteins was inhibited by the relatively long foreign peptide (105 aa) on the loop region. To encapsulate by simultaneous transformation with both NNV capsid gene expressing plasmids and DNA vaccine plasmids (having an eGFP expressing cassette under the CMV promoter), NNV VLPs containing plasmids were produced. The encapsulation of plasmids in the NNV VLPs was demonstrated by PCR and cells exposed to the VLPs encapsulating DNA vaccine plasmids showed fluorescence. These results suggest that the encapsulation of plasmids in NNV VLPs can be done with a simple one-step process, excluding the process of disassembly-reassembly of VLPs, and NNV VLPs can be used as a delivery tool for DNA vaccine vectors.

• BMB Reports
• /
• v.32 no.3
• /
• pp.306-310
• /
• 1999

The partial cDNA of the MT-c clone encoding snake venom metalloprotease was subcloned and expressed in E. coli. The expressed metalloprotease was purified by affinity chromatography in the presence of urea, and then successfully refolded into its functional form, retaining metalloprotease activity that hydrolyzes fibrinogen. The simple and convenient refolding strategy established in this work was highly efficient in recovering the recombinant enzyme activity. Experimental evidence suggests that the C-terminal amino acid stretch of 16 residues is a critical sequence for proper folding of the metalloprotease domain.

• Journal of Plant Biology
• /
• v.38 no.4
• /
• pp.359-364
• /
• 1995

As the first step to elucidate the relationship between the structure and function of CTP:phosphocholine cytidylyltransferase (EC 2.7.7.15) in plants, the partial nucleotide sequence of soybean cytidylyltransferase cDNA was determined using a polymerase chain reaction (PCR). Degenerate oligonucleotide primers were synthesized from the conserved region revealed from the rat and yeast cytidylyltransferase DNA sequences. The catalytic domain region showed 78 and 76% homology with the rat and yeast amino acid sequences, respectivly. The hydropathy profile indicated that the C-terminal non-catalytic portion of the protein was very hydrophilic, and in the region between the catalytic domain and the C-terminal region, there was a large amphipathic $\alpha$-helical domain that was believed to bind the membrane surface in the active formation. There are 7 potential sites for phosphorylation by protein kinase C and 4 potential sites for phosphorylation by Ca2+/calmodulin kinase within the determined sequence.

• The Transactions of the Korea Information Processing Society
• /
• v.7 no.11
• /
• pp.3462-3469
• /
• 2000

In this paper,new image vector quantization method for improvemtnt computation cost and compression ratio is proposed. A proposed method could saved the cornputatio cost of codebook eneration and encoding using partial codebook search, partial codevector elements, and interuplion criterion. And to improve cornpression ratio of codegook index lossless coding, codebook rearrangement, and variable length coding scheme are used.

• Journal of the Korean Institute of Telematics and Electronics C
• /
• v.34C no.2
• /
• pp.29-37
• /
• 1997

A new algorithm and parallel architecture for high-speed complex number multiplication is presented, and a prototype chip based on the proposed approach is designed. By employing redundant binary (RB) arithmetic, an N-bit complex number multiplication is simplified to two RB multiplications (i.e., an addition of N RB partial products), which are responsible for real and imaginary parts, respectively. Also, and efficient RB encoding scheme proposed in this paper enables to generate RB partial products without additional hardware and delay overheads compared with binary partial product generation. The proposed approach leads to a highly parallel architecture with regularity and modularity. As a results, it results in much simpler realization and higher performance than the classical method based on real multipliers and adders. As a test vehicle, a prototype 8-b complex number multiplier core has been fabricated using $0.8\mu\textrm{m}$ CMOS technology. It contains 11,500 transistors on the area of about $1.05 \times 1.34 textrm{mm}^2$. The functional and speed test results show that it can safely operate with 200 MHz clock at $V_{DD}=2.5 V$, and consumes about 90mW.

• Journal of the Optical Society of Korea
• /
• v.19 no.3
• /
• pp.241-247
• /
• 2015

The double-random phase encryption (DRPE) algorithm is a robust technique for image encryption, due to its high speed and encoding a primary image to stationary white noise. Recently it was reported that DRPE in the Fresnel domain can achieve a better avalanche effect than that in Fourier domain, which means DRPE in the Fresnel domain is much safer, to some extent. Consequently, a method based on DRPE in the Fresnel domain would be a good choice. In this paper we present an image-authentication method which uses only partial phase information from a double-random-phase-encrypted image in the Fresnel domain. In this method, only part of the phase information of an image encrypted with DRPE in the Fresnel domain needs to be kept, while other information like amplitude values can be eliminated. Then, with the correct phase keys (we do not consider wavelength and distance as keys here) and a nonlinear correlation algorithm, the encrypted image can be authenticated. Experimental results demonstrate that the encrypted images can be successfully authenticated with this partial phase plus nonlinear correlation technique.

• Asian-Australasian Journal of Animal Sciences
• /
• v.12 no.5
• /
• pp.689-694
• /
• 1999

This experiment was carried out to isolate the partial bovine (Bos Taurus coreanae) myogenic factor encoding gene, MyoD, using the rat myogenic factor (MyoD) gene sequence and to compare the gene sequence between another myogenic factor (Myf 5) and MyoD gene of the bovine. To make the probe and isolate the MyoD gene, PCR was performed to amplify rat and bovine MyoD gene including exon I, II and intron I. The homology between mouse and bovine MyoD is high; bovine MyoD gene shows 17 different gene sequence region compared to rat MyoD. Among those, two regions have significant differences; one is the exon I part between 2834 and 2850 bp, the other is intron part between 3274 and 3303 bp of the mouse. At this region homology was 40% in the former and 50% in the latter. Homology between bovine MyoD and Myf5 was 83% in the exon 1. Especially exon I in the Myf5 602-617 bp and 651-683 bp have significant differences. These results suggest that MyoD gene have a similar gene structure in mouse and bovine and MyoD and Myf5 of the bovine, at least in part, have a similar expression and activity.