• Biomedical Science Letters
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• v.23 no.2
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• pp.96-103
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• 2017

Nocardia species (spp.) are opportunistic pathogen in immunocompromised hosts. The genus Nocardia contains more than 70 species. Nocardia takedensis has been recently reported as a new species of the genus Nocardia. In this study, we describes the first clinical isolate of N. takedensis from the skin lesion in Busan, Korea. For the identification of clinical isolate to the species level as N. takedensis, classical methods (colony morphology, biochemical characteristics, and antimicrobial susceptibility), molecular method (16S rRNA gene sequencing), and MS (mass spectrometry) analysis were conducted. Clinical isolates grew slowly on the culture media (5% sheep blood agar and chocolate agar) under 5% $CO_2$ condition. Especially, carotene pigmentation was detected well on the media. Using mass spectrometry, Nocardia isolate was not identified to the species level. However, molecular method based on 16S rRNA sequencing confirmed the isolate as N. takedensis correctly. N. takedensis isolate was partial positive for acid-fast bacilli on the Ziehl-Neelsen method. And it was observed to be resistance to amoxicillin/clavulanic acid and ciprofloxacin. Our results provide useful information to develop optimal identification protocol of N. takedensis in clinical diagnostic laboratories.

• Tuberculosis and Respiratory Diseases
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• v.39 no.4
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• pp.343-347
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• 1992

Background: There are several causes that the total destruction of unilateral lung can occur. Pulmonary tuberculosis of these causes may lead to destruction through chronic inflammation and fibrosis. In such circumstances, the left to right ratio is supposed to be different. We performed the study with 224 cases for total destruction of unilateral lung, who were admitted at National Kongju Tuberculosis Hospital for recent 4 years. Method: On admission chest film, radiologic pattern was classified into 4 types. And we analyzed patients' age, sex, duration of illness, sputum AFB smear and culture. Result: 1) The male to female ratio was 2:1 and 74% of the patients were between 31 and 60 years of age. 2) One hundred and ninety eight cases(88%) had the duration of illness over 3 years. Namely, most of patients had long history. 3) Sputum AFB smear and/or culture were positive mostly (80%). 4) As for the radiologic pattern, 181 cases (81%) had the total destroyed lung in left and partial lesion in right lung, 31 cases (14%) total destroyed lung in right and partial lesion in left lung, 11 cases (5%) total destroyed lung in left only, and 1 case total destroyed lung in right only. 5) In the radiologic pattern, there is no significant difference in terms of age, sex, duration of illness and sputum examination. Conclusion: From these results, we found that the total destruction of unilateral lung in pulmonary tuberculosis occurred generally in the left side.

• Journal of Food Hygiene and Safety
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• v.8 no.1
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• pp.37-53
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• 1993

F. moniliforme MRC 826, a common fungal contaminant of com, has been known to produce a group of mycotoxins, the fumonisins. By thin layer chromatography, fumonisin $B_{1}$ was detected in the F. moniliforme MRC 826 com culture material(CM) extracts. This study was performed to compare the toxicity and carcinogenicity of F. moniliforme MRC 826 CM with those of aflatoxin $B_1(AFB_1)$ in rats. The toxicity was tested over a period of 7 days in ten female Sprague-Dawley (SD) rats. Treatment group were fed a 1 : 1 mixture(wt/wt) of ground CM and basal diet in powder form, while other negative control group were given basal diet alone. The principal pathological changes in rats treated with 50% CM were hepatocellular hydropic degeneration and renal tubular necrosis. The cancer-promoting activity of CM was evaluated in the rat liver diethylnitrosamine-two thirds partial hepatectomy(DEN-PH) model for carcinogenesis. 70 male SO rats(ca. 170 g) were randomized into 5 groups. Group I served as the positive controls and received the basal diet containing 2 ppm $AFB_{1}$ group 2 received 5% CM, group 3 received 2.5% CM, group 4 received 5% normal com and group 5 received 2.5% normal com. 5% treated group showed cancer promoting activity in rat liver using DEN as initiator and the induction of glutathione S-transferase placental form positive foci as an end point after 6 weeks of promotion.