• The Korea Journal of Herbology
• /
• v.34 no.1
• /
• pp.91-98
• /
• 2019

Objectives : While inducing inflammatory response due to LPS it will investigate mechanism associated with anti-inflammatory effects from macrophages and provide basic data for the possible use as anti-inflammatory materials. Methods : We investigated cell viability, NO, $TNF-{\alpha}$ and IL-6 by ELISA and expressions of iNOS, COX-2, MAPKs and $NF-{\kappa}B$ were measured in RAW 264.7 cells induced by LPS. Results : The cell viability of Parthenocissus tricuspidata extracts(PTE) identified in macrophages showed that cell viability rate was more than 99% at the concentration of 8, 40, and $200{\mu}g/mL$. NO generated amounts revealed that it relied on concentration and was significantly reduced compared to the control. The expression of iNOS was restrained by the control at the concentration of 200 and $400{\mu}g/mL$. In addition, the expression of COX-2 was found to be significantly reduced to the untreated control at the concentration of $400{\mu}g/mL$. $TNF-{\alpha}$ relied on concentration and showed a significant decreased compared to the control. In contrast, IL-6 relied on concentration, reduced compared to the control. Phosphorylation of ERK, JNK, and p38 mediated by LPS were restrained by relying on concentration. Phosphorylation and decomposition of $I{\kappa}B{\alpha}$ as well as p65 nuclear transmission of $NF-{\kappa}B$ subunit were restrained. Conclusions : By restraining the activation of $NF-{\kappa}B$, anti-inflammatory effects were revealed by reducing phosphorylative activation of MAPKs, restraining the expression of iNOS and COX-2 and restraining the creation of NO, IL-6, and $TNF-{\alpha}$. Therefore, it can be assumed that they can be used as a variety of anti-inflammatory materials.

• Archives of Pharmacal Research
• /
• v.27 no.3
• /
• pp.300-304
• /
• 2004

Five caffeic acid derivatives; methyl ester of caffeoylglycolic acid (1), dimethyl ester of caffeoyltartaric acid (2), dimethyl ester of caffeoyltartronic acid (3), monomethyl ester of caffeoyltartronic acid (4), methyl ester of caffeic acid (5), and some other secondary metabolites including; quercetin, quercetin 3-O-$\beta$-D-glucuronide methyl ester, kaempferol, 3,5,7,4'-O-tetramethylkaempferol, $\beta$-sitosterol glucoside, 2$\alpha$-hydroxyursolic acid and 2,24-dihydroxyursolic acid, have been isolated and characterized. All the isolated compounds were characterized with the help of NMR spectroscopy and mass spectrometry. Structure of compound 3 was also confirmed by a single X-ray crystallographic technique. Isolates were evaluated for anti-oxidant activities and most of the tested compounds were found to be potent in DPPH free radical scavenging ($IC_{50}{\;}={\;}4.56-14.17{\;}{\mu\textrm{g}}/mL$) and superoxide anion scavenging ($IC_{50}{\;}={\;}0.58-7.39{\;}{\mu\textrm{g}}/mL$) assays.

• Journal of Korean Society of Forest Science
• /
• v.37 no.1
• /
• pp.1-16
• /
• 1978

The study describes on the identification and morphological characteristics of each species, ecological characteristics and propagation techniques, and developing utilization measures of the Parthenocissus plants for environment conservation and revegetation of the babe rock-slopes. The following species and varieties are disscussed in this study; Parthenocissus tricuspidata (S. et Z.) Planch. var. veitchii Rehd. var. lowii Rehd. var. pupurea Hort. Parthenocissus quiquefolia (L.) Planch. var. engelmanni Rehd. var. saint-pauli Rehd. var. hirsuta Planch. Parthenocissus henryana Diels et Gilg. Parthenocissus thomsoni Planch. Parthenocissus heptaphylla Small. Parthenocissus inserta (Kern.) K. Fritsch. Parthenocissus laetevirens Rehd. Parthenocissus himalayana Planch. These are, in general, all vigorous self-clinging climbers that will quickly cover a wall and bare rock surfaces with a dense network of branch growths and beautiful green leaves which change to shades of scarlet and crimson before they fall in Autumn. Parthenocissus tricuspidata out of 8 species in the genus Parthenocissus is the most useful plant for the environment conservation including the bare rock-slope revegetation and for the production of food and shelter for wildlifes. This native of Korea clings by means of small rootlike holdfasts (adhesive discs) and holds (tendrils) to stone work or any other solid support, tenaciously.

• Mycobiology
• /
• v.50 no.3
• /
• pp.166-171
• /
• 2022

Virginia creeper (or five-leaved ivy; Parthenocissus quinquefolia) is one of the most popular and widely grown climbers worldwide. In September 2021, Virginia creeper leaves with typical rust symptom were found in an arboretum in Korea, with severe damage. Globally, there is no record of a rust disease on Virginia creeper. Using morphological investigation and molecular phylogenetic inferences, the rust agent was identified as Neophysopella vitis, which is a rust pathogen of other Parthenocissus spp. including Boston ivy (P. tricuspidata). Given that the two ivy plants, Virginia creeper and Boston ivy, have common habitats, especially on buildings and walls, throughout Korea, and that N. vitis is a ubiquitous rust species affecting Boston ivy in Korea, it is speculated that the host range of N. vitis may recently have expanded from Boston ivy to Virginia creeper. The present study reports a globally new rust disease on Virginia creeper, which could be a major threat to the ornamental creeper.

• Research in Plant Disease
• /
• v.29 no.3
• /
• pp.299-303
• /
• 2023

Rust symptoms on Meliosma myriantha trees have been noticed during disease surveys in Korea since 2010, with a high disease incidence frequently surpassing 90%. The causal fungus of the rust disease was identified as Neophysopella vitis based on the morphological investigation and molecular sequence analysis of the internal transcribed spacer (ITS) and large subunit (LSU) rDNA regions. This is the first report of rust disease caused by N. vitis on M. myriantha in Korea. A pathogenicity assay proved that M. myriantha serves as the aecial host of N. vitis as spermogonia and aeciospores were produced, which can infect the two uredinial hosts, Boston ivy (Parthenocissus tricuspidata) and Virginia creeper (Parthenocissus quinquefolia).

• Journal of Life Science
• /
• v.23 no.3
• /
• pp.399-405
• /
• 2013

This study was carried out to analyze the effects on antioxidative, antidiabetic, and anti-inflammatory activities of Parthenocissus tricuspidata (PT) stem extracts. The total phenolic contents of hot water and ethanol extracts from PT stems were 61.5 mg TAE/g and 122.1 mg TAE/g, respectively. The antioxidative activities of hot water and ethanol extracts from PT stem were measured by using 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity and superoxide dismutase (SOD) assay. The DPPH radical scavenging activities of ethanol extract and butanol fraction were approximately 95% and 92% at 1 mg/ml, respectively, and the SOD activities of ethanol extract and butanol fraction were about 91% and 97% at 1 mg/ml, respectively. The DPPH radical scavenging and SOD activities of ethanol extract and butanol fraction from PT stem increased remarkably increased in a dose-dependent manner and were higher than in the hot water extracts. Compared to the acarbose, a known anti-diabetic drug, which was used as a positive control, the ${\alpha}$-glucosidase inhibitory capacity of PT stem showed a strong inhibitory rate in ethanol extract and in butanol and hexane fractions. We investigated the effect of hot water extract from PT stem on lipopolysaccharide (LPS)-induced nitric oxide (NO) production in RAW 264.7 cells. Hot water extract from PT stem inhibited LPS-induced NO production up to 40% at a treatment of 1 mg/ml. These results suggest that PT stem extracts have an effect on antioxidative, antidiabetic, and anti-inflammatory activities and thus have great potential as antidiabetic materials and a source for natural health products.

• Korean Journal of Plant Resources
• /
• v.14 no.2
• /
• pp.116-123
• /
• 2001

The non-enzymatic antioxidants and antioxidant enzyme from the extracts of Parthenocissus tricuspidata PLANCH. were examined in order to utilize natural product for cerchemopreventive agents. The antioxidant potential and enzyme activities on plant positions in the extracts of Parthenocisuss tricuspidata PLANCH. showed considerable differences. The antioxidant activity of the leaf extracts by Ethyl acetate fractions of Parthenocisuss tricuspidata PLANCH. was the highest among three positions ($7.57\mu\textrm{g}/m\ell$). The highest activities showed in S-5 (in leaf), S-4 (in stem) and S-3 (in root) fraction by Silicagel column chromatography and the antioxidant activity showed, in purified extract of each positions, $7.06\mu\textrm{g}/m\ell$ (in leaf), $6.99\mu\textrm{g}/m\ell$ (in stem) and $12.39\mu\textrm{g}/m\ell$ (in root) respectively. The activities of DPPH by LH-20 column chromatography revealed much higher than those by silica-gel column chromatography. These were identified as the phenolic compounds known as antioxidant compounds such as Benzoic acid(Gallic acid), 1-methyl-3-(2-phenylethen) benzene, phloroglucinol and 1,2-dihydroxy-4-(1-propyl)benzene by GC/MS. POD activities in the stem and root were higher than in the leaf. SOD activity was highest in the leaf, stem and root activity was comparatively low. Especially, SOD activity in leaf was over 2 times higher than root.

• Korean Journal of Environmental Agriculture
• /
• v.17 no.4
• /
• pp.358-361
• /
• 1998

This study was carried out to develop a short term hardening method of tree seedlings of Rhus chinensis Mill., Evodia daniellii Hemsley and Parthenocissus tricuspidata(Sieb. et Zucc.) Planck for afforestation on a concave and a crack of cut-rock slope. The seedlings were grown in a cylinder shaped pot made of polyvinyl net with the soil media of peatmoss, vermiculite, clay, compost, fertilizer, and absorbant(40:25:19:15:1:0.1, v:v). They were cultivated in a greenhouse for four months and in field condition for two months. During the last three months of the growing period the seedlings were hardened by periodic desiccation and irrigation in 4 to 10 days interval. The hardened seedlings showed lower leaf water potential, higher leaf osmotic pressure, and lower T/R ratio than those before the hardening. The hardened seedlings survived well on the soil medium in the concave of cut-rock slope.

• Journal of the Korean Institute of Landscape Architecture
• /
• v.49 no.5
• /
• pp.71-78
• /
• 2021

Green areas and the area of available horizontal surfaces are gradually decreasing due to the overcrowding of buildings. It is adversely affecting the urban climate and ecosystem. However, the recognition of the importance of green areas is gradually increasing. As a result, the importance of wall greening using vertical surfaces is growing. However, despite the fact that domestic wall greening guidelines and institutions related to orientations restrict planting. there was no study to determine whether there were actual differences in plant growth due to orientations. Therefore, this study compared and analyzed the plant growth characteristics by orientations to apply actual wall greening to cities. The experiment was conducted from May to September 2020. First of all, three octave walls were constructed to measure the temperature, the illumination, and the length of the plants once a week. The plants included Parthenocissus tricuspidata, Hedera rhombea, and Euonymus radicans cv. Aueonmarinata Rehd plants. As a result of the study, Parthenocissus tricuspidata was prolific in the north, and Hedera rhombea, and Euonymus radicans cv. Aueonmarinata Rehd plants were prolific in the south. All three types of plants were prolific in June-July, and the Parthenocissus tricuspidata was prolific in grass-growing, and in August, all the walls were 100% covered. Hedera rhombea had the lowest rate of herbaceous growth, and the vertical coverate was also lower at an average of 45%, but among the three plants, the sheath of the horizontal surface coverate was the highest. Euonymus radicans cv. Aueonmarinata Rehd was low in the speed of herbaceous growth, and finally, the walls were 100% covered except for the north and northwest directions. It was found that not all plants used for wall greening show the same growth, and the difference in growth varies more depending on plants than the effect of orientations. Therefore, it is better to identify the characteristics of plant growth and plant suitable plants for each directions.

• Journal of the Society of Cosmetic Scientists of Korea
• /
• v.43 no.3
• /
• pp.255-263
• /
• 2017

In this study, the antioxidant activities, cellular protective effects, and inhibitory effects on elastase of non-fermented and fermented extracts of Parthenocissus tricuspidata (P. tricuspidata) stem using Lactobacillus pentosus were investigated. The free radical scavenging activities ($FSC_{50}$) of non-fermented and fermented extracts were 42.3 and $34.5{\mu}g/mL$, respectively, in which the activity after fermentation was approximately 18.4% higher. Reactive oxygen species (ROS) scavenging activities ($OSC_{50}$) in $Fe^{3+}-EDTA/H_2O_2$ system of non-fermented and fermented extracts were 2.6 and $2.5{\mu}g/mL$, respectively. The activity after fermentation was approximately 4.2% higher. In the $^1O_2$-induced cellular damage of erythrocytes, the cellular protective effects (${\tau}_{50}$) of non-fermented and fermented extracts were 126.4 and 173.0 min at $50{\mu}g/mL$, respectively. The activity after fermentation was approximately 34.0% higher. The effect of fermented extract was 3.9 times higher than $(+)-{\alpha}$-tocopherol (${\tau}_{50}=43.4min$), known as a lipophilic antioxidant at $50{\mu}g/mL$. The inhibitory effect of elastase was investigated to predict the anti-wrinkle efficacy using Hs68 human fibroblasts cells. The elastase inhibitory activities ($IC_{50}$) of non-fermented and fermented extracts were 873.6 and $687.8{\mu}g/mL$, respectively, and the activity after fermentation was approximately 21.3% higher. These results indicated that fermented extract of P. tricuspidata stem has potentials as natural cosmetic ingredients with antioxidant and anti-wrinkle effect.