• 대한치과교정학회지
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• 제30권4호
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• pp.467-473
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• 2000

각형 호선이 edgewise 브라켓에 삽입되면 first, second order는 bending에 의해, third order는 torsion(비틀림)에 의해 3차원적인 force system이 발생한다. Bending에 관하여는 분석적 그리고 실험적인 많은 연구가 보고 되어 있는 반면 비틀림에 관해서는 상대적으로 많은 연구가 이루어 지지 않았다. 본 연구의 목적은 각형 와이어의 재료와 단면의 형태가 와이어의 비틀림 모멘트에 어떻게 영향을 주는지를 이론적, 실험적으로 밝혀서 임상적으로 적절한 모멘트를 가할 때 호선의 재료와 굵기를 합리적으로 선택할 수 있도록 하는데 있다. 실험재료로는 third order조절을 위해 가장 많이 사용하는 호선을 사용하였다. 크기별로 0.016x0.022, 0.017x0.025, 0.019x0.025인치 그리고 재료로는 stainless steel (Ormco), TMA(Ormco), NiTi(Ormco), 그리고 braided stainless steel(DentaFlex, Dentauum) 네 가지를 사용하여 총 12개의 조합을 사용하였다. Torsion formula를 이용하여 비틀림 강성 (torque/twist rate)을 계산하였고 torque gauge를 이용하여 비틀림 강성, 항복 비틀림 모멘트 (yield torsional moment), 그리고 최대 비틀림 모멘트 (ultimate torsional moment)를 측정하였다. Torsion formula에 의하면 비틀림 강성 (T/$\theta$)은 재료적인 특성(G)과 호선의 단면의 특성(J)에 비례하고 호선의 길이(L)에 반비례한다. 대부분의 실험치는 이론적인 값과 비슷하게 나타났다.

• 디지털융복합연구
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• 제10권3호
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• pp.13-21
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• 2012

본 연구의 주목적은 전자상거래 환경 하에서 지각된 위험요인이 고객의 지각된 가치와 재구매의도에 미치는 영향을 분석해 보는 것이다. 연구목적을 달성하기 위하여 본 연구에서는 선행문헌 고찰결과를 토대로 전자상거래 이용자의 지각된 위험으로 프라이버시 위험, 시간적 위험, 경제적 위험, 심리적 위험, 상품성능 위험 등 여섯 가지 요인을 설정하였고, 전자상거래 성과를 측정하기 위하여 지각된 가치와 재구매의도를 포함한 연구모형을 구축하였다. 설문조사 결과, 전자상거래 이용자 174명으로부터 자료가 수집되었으며, 공분산 구조모델 분석을 통해 가설검정을 수행하였다. 연구결과는 다음과 같이 요약해 볼 수 있다. 첫째, 지각된 위험 중 프라이버시 위험, 경제적 위험, 심리적 위험, 상품성능 위험 등 네 가지 요인이 지각된 가치에 유의한 영향을 미치는 것으로 나타났다. 둘째, 지각된 가치는 전자상거래 쇼핑몰의 재구매의도에 매우 유의한 영향을 미치는 것으로 밝혀졌다. 연구결과를 토대로 전자상거래 환경에서 고객의 지각된 가치와 재구매의도를 향상시키기 위한 전략에 대한 논의가 이루어졌으며, 연구의 시사점과 향후 연구방향을 제시하였다.

• Molecular & Cellular Toxicology
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• 제4권1호
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• pp.16-21
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• 2008

Cell death of trophoblast, particularly by abnormal release of physiological nitric oxide (NO) has been known to be a causative factor of pre-eclampsia. In the present study, effects of intracellular calcium increase enhancing the activity of NO synthases (neuronal NO synthase, nNOS in this trophoblast cells) on the cell death were examined in a human placental full-term cell line (HT-1). Furthermore, we analyzed the possible mechanisms underlying the augmentation of $Ca^{++}$-mediated NOS activity mediated by protein kinases like PKC, PKA, or CaM-KII. In experiments for cell toxicity, a calcium ionophore (ionomycin $10{\mu}M$) enhanced cell death confirmed by MTT assay, and increased significantly nNOS activity determined with a hemoglobin oxidation assay. This cell death was partially protected by pre-treatment of 7-nitroindazole (7-NI, $10{\mu}M$ and $100{\mu}M$), a nNOS-specific inhibitor. Additionally, $Ca^{++}$-ionophore -induced increase of nNOS activity also was partially normalized by pre-treatment of specific inhibitors of protein kinases, PKC, PKA or CaM-KII. Therefore, we suggest that an increase of calcium influx, leading to the activation of nNOS activity, which in turn may result in the death of trophoblast cells by involvement of signaling mechanisms of protein kinases.

• 한국신재생에너지학회:학술대회논문집
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• 한국신재생에너지학회 2007년도 춘계학술대회
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• pp.141-145
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• 2007

Due to their excellent catalytic activity with respect to methanol oxidation on platinum at low temperature, platinum nanosized catalysts have been a topic of great interest for use in direct methanol fuel cells (DMFCs). Since pure platinum is readily poisoned by CO, a by-product of methanol electrooxidation, and is extremely expensive, a number of efforts to design and characterize Pt-based alloy nanosized catalysts or Pt nanophase-support composites have been attempted in order to reduce or relieve the CO poisoning effect. In this review paper, we summarize these efforts based upon our recent research results. The Pt-based nanocatalysts were designed by chemical synthesis and thin-film technology, and were characterized by a variety of analyses. According to bifunctional mechanism, it was concluded that good alloy formation with $2^{nd}$ metal (e.g., Ru) as well as the metallic state and optimum portion of Ru element in the anode catalyst contribute to an enhanced catalytic activity for methanol electrooxidation. In addition, we found that the modified electronic properties of platinum in Pt alloy electrodes as well as the surface and bulk structure of Pt alloys with a proper composition could be attributed to a higher catalytic activity for methanol electooxdation. Proton conducting contribution of nanosized electrocatalysts should also be considered to be excellent in methanol electrooxidation (Spillover effect). Finally, we confirmed the ensemble effect, which combined all above effects, in Pt-based nanocatalsyts especially, such as PtRuRhNi and $PtRuWO_{3}$, contribute to an enhanced catalytic activity.

• 한국수소및신에너지학회논문집
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• 제18권4호
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• pp.356-364
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• 2007

The partial reduction and water splitting properties of metal substituted ferrite mediums for two-step thermochemical hydrogen production, were carried out by TPR/O(Temperature programmed reduction/oxidation) method at a temperature of below 1173 K and under atmospheric pressure. $ZrO_2$ was added to the ferrite as a binder to prevent the sintering. As the results, the reactivity of the metal species added to the ferrite mediums decreased in the order of Cu>Co>Ni>Mn, on the basis of water-splitting temperature. It was also found that the produced hydrogen amounts in the water-splitting step on partial reduced mediums were corresponding to the consumed hydrogen amounts in the previously partial reduction step.

• 대한화학회지
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• 제30권2호
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• pp.216-223
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• 1986

Dansyl 유도화된 아미노산의 광학이성질체를 분리하기 위해 광학활성인 L-arginine과 몇가지 금속(구리, 아연, 카드뮴, 니켈)의 킬레이트를 이동상에 첨가하여 역상 칼럼내에서 분리를 시도했다. 구리 킬레이트 이외의 것은 광학이성질체의 분리가 안되었다. 아미노산의 광학이성질체의 분리 거동은 이동상의 pH 및 유기용매조성, 완충용액의 종류와 농도, 금속의 종류와 킬레이트 농도에 의해 영향을 받음을 알 수 있었다. Valine, metionine, leucine, phenylalanine은 D형이 먼저 용리되고 serine과 alanine은 L형이 먼저 용리되었으며 threonine은 D형과 L형의 분리현상이 나타나지 않았다. 이러한 분리거동은 리간드 교환반응에 의한 (D, L-DNS-AA) (M) (L-Arg) 3종류 착물이 형성될때의 입체 특이성효과로써 설명할 수 있다.

• 대한조선학회논문집
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• 제51권3호
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• pp.246-253
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• 2014

Liquid natural gas is stored and transported inside cargo tank which is made of specially designed cryogenic materials such as 9% Ni steel, Al5083-O alloy and SUS304 and so on. The materials have to keep excellent ductile characteristics under the cryogenic environment, down to -163oC, in order to avoid the catastrophic sudden brittle fracture during the operation condition. High manganese steel is considered to be the promising alternative material that can replace the commonly used materials mentioned above owing to its cost effectiveness. In line with this industrial need, the mechanical properties of the high manganese steel under both room and cryogenic environment were investigated in this study focused on its tensile and fatigue behavior. In terms of the tensile strength, the ultimate tensile strength of the base material of the high manganese steel was comparable to the existing cryogenic materials, but it turned out to be undermatched one when welding is involved in. The fatigue strength of the high manganese steel under room temperature was as good as other cryogenic materials, but under cryogenic environment, slightly less than others though better than Al 5083-O alloy.

• 한국환경복원기술학회지
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• 제16권1호
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• pp.27-53
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• 2013

This study is carried out to offer raw data and establish strategy for conservation and restoration of river ecosystem by objective surveying and analysing the flora and vegetation distributed in Hwang river, Gyeongsangnam-do. The flora identified in this site were 406 taxa including 95 families, 252 genera, 360 species, 3 subspecies, 40 varieties and 3 forms. The rare plants were 3 taxa including Sagittaria sagittifolia subsp. leucopetala, Hydrocharis dubia and Acorus calamus. The endemic plants were 4 taxa including Populus tomentiglandulosa, Salix koriyanagi, Paulownia coreana and Weigela subsessilis. The specific plants by floristic region were 17 taxa including Salix siuzevii, Poncirus trifoliata, Potamogeton maackianus and so forth. The naturalized plants were 39 taxa including Phytolacca americana, Chenopodium glaucum, Lepidium virginicum, Oenothera erythrosepala, Xanthium canadense, Lolium multiflorum and so forth. The invasive alien plants were Ambrosia artemisiifolia and Ambrosia trifida. The whole NI and UI were each 9.6% and 13.9%. The hydrophytes were 27 taxa including Marsilea quadrifolia, Nymphaea tetragona, Ceratophyllum demersum, Trapa japonica and so forth. In the results of growth forms of the hydrophytes, emergent species were 14 taxa, 3 taxa of floating-leaved species, 4 taxa of free-floating species and 6 taxa of submerged species. The dominant vegetations were Salix spp. community, Miscanthus sacchariflorus community, Phragmites japonica community and Phragmites communis community.

• Journal of Microbiology and Biotechnology
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• 제11권5호
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• pp.845-852
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• 2001

A microorganism producing fibrinolytic enzyme was isolated from Korean traditional soybean paste and identified as Bacillus sp. KDO-13. The fibrinolytic enzyme was purified to homogeneity by ammonium sulfate fractionation, ion-exchange chromatography on DEAE-celluose, and gel chromatography on Sephadex G-100 of the culture supernatant of Bacillus sp. KDO-13. The molecular weight of the purified enzyme was estimated to be 44,000 by SDS-PAGE. The optimum pH and temperature for the enzyme activity were pH 8.0 and $50{\circ}C$, respectively. The enzyme activity was relatively stable at pH 7.0-9.0 and temperature below $50{\circ}C$. the activity of the enzyme was inhibited by $AI^{3+}$ and $Hg^{2+}$, but activated by $Co^{2+}$\;and\;Ni^{2+}. In addition, the enzyme activity was potently inhibited by EDTA and 0-phenanthroline. The purified enzyme could completely hydrolyze a fibrin substrate within 6 h in vitro, and had a low $K_m$ value for fibrin hydrolysis. It was concluded that the purified enzyme was a metalloprotease with relatively high specificity for fibrinolysis, and thus, could be applied as an effective thrombolytic agent.

• Journal of Life Science
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• 제9권1호
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• pp.26-34
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• 1999

A thermostable pullulanase has been isolated and purified from Thermus caldophilus GK-24 to a homogeneity by gel-filtration and ion-exchange chromatography. The specific activity of the purified enzyme was 431-fold increase from the crude culture broth with a recovery of 11.4%. The purified enzyme showed $M_{r}$ of 65 kDa on denaturated and natural conditions. The pI of the enzyme was 6.1 and Schiff staining was negative, suggesting that the enzyme is not a glycoprotein. The enzyme was most active at pH 5.5. The activity was maximal at $75^{\cire}C$ and stable up to $95^{\cire}C$ for 30 min at pH 5.5. The enzyme was stable to incubation from pH 3.5 to pH 8.0 at $4^{\cire}C$ for 24hr. The presence of pullulan protected the enzyme from heat inactivation, the extent depending upon the substrate concentration. The activity of the enzyme was simulated by $Mn^{2+}$ ion, }$Ni^{2+}$, $Ca^{2+}$, $Co^{2+}$ ions. The enzyme hydrolyzed the ${\alpha}$-1,6-linkages of amylopectin, glycogens, ${\alpha}$, ${\beta}$-limited dextrin, and pullulan. The enzyme caused the complete hydrolysis of pullulan to maltotriose and the activity was inhibited by $\alpha$, $\beta$, or $\gamma$-cyclodextrins. The $NH_{2}$-terminal amino acid sequence [(Ala-Pro-Gln-(Asp of Tyr)-Asn-Leu-Leu-Xaa-ILe-Gly-Ala(Ser)] was compared with known sequences of various sources and that was compared with known sequences of various sources and that was different from those of bacterial and plant enzymes, suggesting that the enzymes are structurally different.