• Journal of Pharmacopuncture
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• v.7 no.1 s.12
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• pp.5-14
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• 2004

Acupuncture has fairly good weight-reducing effect in treating simple obesity due to the neuroendocrine regulation. In this study, the antiobesity effects of herbal acupuncture(HA) with Sang-hwang(phellinus linteus) at Fuai(SP16) were investigated in the rat fed on high-fat(HF) diet. Sang-hwang mushroom has been proven to have anti-carcinogenic effects and Sang-hwang extracts are highly effective in treatment and preventive treatment of AIDS, diabetes and high blood-pressure. To determine whether the Sang-hwang herbal acupuncture may have the anti-obesity effect, male Sprague-Dawley(4-wk-old) rats were fed a HF diet for 5 wk, which produced significant weight gain compared to rats were fed a normal diet, and then herbal acupuncture were treated for 3 wk in HF diet group. The body weight, food consumption, food effeciency ratio(FER), body fat mass, plasma nitric oxide(NO) were investigated in rats fed on normal diet, HF diet, and HF diet with HA(HF-diet-HA) groups. NO has been proposed to be involved in the regulation of food intake. In addition, the expression of appetite peptides such as orexigenic peptide neuropeptide Y(NPY) and the anorectic peptide cholecystokinin(CCK) were observed in the hypothalamus. HF-HA group reduced body weight gain, FER, body fat contents and NO concentration compared to HF diet group. The expression of NPY was reduced in arcuate nucleus(ARC), and CCK was increased in the paraventricular nucleus(PVN) after treatment of HA. In conclusion, Sang-hwang HA reduced adipocity, plasma NO and hypothalamic NPY, but increased CCK expression in the HF dietinduced obesity rat, therefore HA may have anti-obesity action through regulating body weight and appetite peptide of the central nervous system.

• Korean Journal of Acupuncture
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• v.41 no.3
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• pp.79-89
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• 2024

Objectives : We aimed to identify the effects of acupuncture treatment on alleviating pain and depression and modulating brain neural activity in the neuropathic pain and depression comorbidity mouse model (PDCM). Methods : We performed partial sciatic nerve ligation on the right hind paw of mice to induce neuropathic pain and injected reserpine (2 mg/kg, intraperitoneal) for 10 days from the day of the surgery to induce pain and depression. Acupuncture treatment was performed for 10 days at the following locations: 1) ST36 and SP6 (Joksamni and Sameumgyo; JS), 2) KI1 and HT7 (Yongcheon and Sinmun; YS), 3) LR1, PC9, KI10, and PC3 (Pericardium tonification; PT), or 4) LR1, HT9, KI10, and HT3 (Heart tonification; HT). Pain-like behavior was measured using the von Frey test and depressive-like behavior was assessed using the open field test. Then, the c-Fos expression was analyzed in the brain regions of neocortex, striatum, hypothalamus, hippocampus, midbrain, and medulla to examine brain neural activity. Results : In PDCM, pain-like behavior was alleviated by acupuncture treatment on the JS, YS, PT, and HT, and depressive-like behavior was improved by acupuncture treatment on the JS and YS. JS and YS were derived as an optimized acupoint combination for improving neuropathic pain and depression comorbidity. Brain neural activities in the neocortex (infralimbic cortex) and hypothalamus (paraventricular hypothalamic nucleus; PVN) were commonly altered by both JS and YS acupuncture treatments. In addition, neural activities in the neocortex (prelimbic cortex; PrL) and midbrain (substantia nigra, lateral part of the dorsal raphe nucleus) were altered exclusively by JS acupuncture treatment, while changes in the area 2 of the anterior cingulate cortex and the cornu ammonis 3 of the hippocampus were specific to YS acupuncture treatment. Brain neural activity in the PrL and PVN regions was significantly correlated with changes in pain behavior. Conclusions : Both JS and YS acupuncture treatments alleviated pain and depressive-like behaviors, which were associated with modulation of neural activities in the neocortex, hypothalamus, hippocampus, and midbrain.

• Korean Journal of Acupuncture
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• v.24 no.4
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• pp.221-231
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• 2007

목 적 : 본 연구의 목적은 시상하부에서 침처치에 대한 nitric oxide synthase (NOS)발현을 nicotinamide adenine dinucleotide phosphate-diaphorase (NADPH-d)를 이용한 조직화학 염색법으로 관찰하였다. 실험방법 : 동물은 Balb/c (wild type) 와 Stat 4 knockout (KO) 생쥐를 사용하였다. 염증유도는 1% carrageenan 용액 (20ul/마리)을 발 뒤꿈치 표피에 주사하였고, 침 처치는 족삼리 (ST36)에 시침하였다. 침 처치 후 5시간까지 부종율을 부종측정기로 측정하였으며, 마지막으로 부종을 측정한 후 동물을 희생하여 뇌를 적출하여 고정하였다. 침에 대한 효과를 확인하기 위하여 NADPH-d 반응의 조직염색을 실시하였다. 염증유도와 그룹간의 유의성 검증은 one-way ANOVA를 사용하였다. 결 과 : 대조군인 Balb/c와 실험군인 stat4 KO 생쥐를 carrageenan으로 염증을 유도시에 대조군은 90%이상 유도된 반면, Stat4 KO 그룹은 50% 정도의 염증만이 유도되었다. 염증을 유도한 생쥐의 족삼리에 침 처치시 대조군은 1시간에서 약 40%정도 감소하였고 (P<0.05), Stat4 KO 실험군은 유의한 염증 감소율을 보이지 않았다. 시상하부의 lateral hypothalamic area (LHA)와 paraventricular nucleus (PVN)부위의 침에 대한 효과를 NADPH-d 에 양성으로 반응하는 세포수로 비교하여 다음과 같은 결과를 얻었다. (1) 대조군에서 염증 유도시 시상하부의 PVN는 NADPH-d 양성세포수가 감소하였고, LHA에서는 증가하였다. (2) 염증을 유도한 대조군에 침을 처치시 PVN은 세포수가 증가하였고, LHA에서는 감소하는 경향을 보였다. (3) 염증을 유도한 Stat4 KO 군에서는 시상하부의 PVN과 LHA부위 모두에서 NADPH-d 양성세포수가 감소하였고, 염증유도그룹에 침을 처치시 PVN과 LHA부위 모두에서 세포수가 증가함을 관찰 할 수 있었다. (4) 대조군과 실험군 모두에 salicylic acid로 비교하였더니 염증유도 효과 및 NADPH-d 세포 수에서 침 처치와 비슷한 결과를 나타내었다. 결 론 : 침은 염증을 유도한 생쥐에서 염증 감소에 유의한 효과가 있다. 염증을 유도한 Balb/c 와 Stat4 KO 생쥐에 침을 처치 시 시상하부의 NADPH-d 발현이 LHA부위와 PVN에서 서로 다르게 나타나는 것으로 나타난다. 이러한 현상은 침 효과가 시상하부의 위치에 대한 작용이 다르기 때문이라고 생각된다.