• Journal of the Korean Association of Oral and Maxillofacial Surgeons
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• 제31권1호
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• pp.24-30
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• 2005

타액선에서 교감 신경과 부교감 신경이 나트륨 운반체와 수분 통로의 조절에 어떠한 기능을 하는지 알아 보고자 흰쥐 악하선을 지배하는 교감 신경과 부교감 신경을 절제하고 나서 타액선내 나트륨 운반체와 수분 통로의 발현을 조사하여 다음과 같은 성적을 얻었다. 1. Na,K-ATPase의 ${\alpha}1,\;{\beta}1$ 소단위는 교감 신경 절제에 의해 크게 영향받지 않았으나 부교감 신경 절제에 의해서는 두 소단위의 발현이 모두 감소되었다. 2. ENaC ${\alpha}-,\;{\beta}-,\;{\gamma}-$ 소단위는 그 발현이 교감 신경 절제에 의해 영향 받지 않았으나 부교감 신경 절제에 의해서 도리어 증가하였다. 3. NHE3는 교감 신경 및 부교감 신경 절제에 의해 모두 크게 감소했다. 4. 교감 신경 절제시 AQP1의 발현이 크게 증가했으나, 부교감 신경 절제시 영향 받지 않았다. 5. 교감 신경 절제 및 부교감 신경 절제는 AQP4 발현을 크게 증가시켰다. 6. AQP5는 교감 신경 절제시 영향을 받지 않았으나, 부교감 신경 절제시 크게 감소되었다. 이상의 실험성적을 요약할 때 악하선을 지배하는 교감 신경 및 부교감 신경은 선의 나트륨 운반체 및 수분 통로 발현에 긴장성 조절을 영위함으로써 타액의 전해질 및 수분 조성을 결정하는 데 공헌함을 알 수 있다. 그리고 이 신경의 절제시 보이는 나트륨 운반체와 수분 통로의 변화는 기능적으로 신경 절제 타액선에서의 타액 분비량과 타액의 무기질 조성 변화의 원인이 될 것이라 생각된다.

• Journal of the Korean Association of Oral and Maxillofacial Surgeons
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• 제32권1호
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• pp.1-7
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• 2006

The present study was aimed to explore the role of sympathetic and parasympathetic nerves in the regulation of sodium transporters and water channels in the salivary gland. Rats were denervated of their sympathetic and parasympathetic nerves to the submandibular gland, and the glandular expression of sodium transporters and water channels was determined by Western blot analysis. The expression of either ${\alpha}1$ or ${\beta}1$ subunit of Na, K-ATPase was not significantly affected either by the sympathetic or by the parasympathetic denervation. The expression of subunits of epithelial sodium channels was significantly increased both in the denervated and contralateral glands either by the sympathetic or by the parasympathetic denervation. Neither the sympathetic nor the parasympathetic denervation significantly altered the expression of aquaporin-1 (AQP1). Nor was the expression of AQP4 affected significantly by the parasympathetic or the sympathetic denervation. On the contrary, the expression of AQP5 was significantly increased not only by the parasympathetic but also by the sympathetic denervation. These results suggest that sympathetic and parasympathetic nerves have tonic regulatory effects on the regulation of certain sodium transporters and AQP water channels in the salivary gland.

• The Korean Journal of Physiology and Pharmacology
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• 제7권2호
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• pp.97-101
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• 2003

The salivary glands produce 1.5L of fluid per day. As in other exocrine organs, the general mechanism in the salivary glands is that water movement occurs secondary to osmotic driving forces created by active salt transport. Therefore, high water permeability in the salivary glands is expected to have a variety of aquaporin (AQP), a water channel. Although some AQPs have been known to be present in the salivary glands, roles of parasympathetic nerve in AQP expression have not yet been examined. This study was designed to examine the changes of AQPs and extracellular signal-regulated kinase (ERK) in the submandibular glands after parasympathetic denervation. Right chorda-lingual nerve was cut, and each right (experiment) and left (control) submandibular gland was excised at 1, 3, 7, 14, 30 days after denervation. The denervated right submandibular glands were resulted in weight loss and morphologic changes, including cell loss and atrophy, as the time elapsed after parasympathetic denervation increased, whereas there were no histologic alteration in control side. AQP5 which is known to reside in apical membrane and secretory caraliculi of the submandibular acini were gradually underexpressed according, as the time after denervation increased. Expression of AQP4 in submandibular ductal epithelium was down-regulated after denervation. Besides, AQP3 and 8, which is known to be present in basolateral membrane of the glandular acini, were gradually underexpressed after denervation, similar to the pattern of other types. Expression of ERK, a mitogen-activated protein kinase, was downregulated after parasympathetic denervation in the submandibular gland. These results suggest that parasympathetic nervous system regulates the expression of AQPs in salivary glands, and is in part mediated by ERK pathway.

• The Korean Journal of Physiology and Pharmacology
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• 제10권2호
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• pp.65-69
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• 2006

The present study was undertaken to explore the role of autonomic nerves in the regulation of sodium transporters and water channels in the salivary gland. Rats were denervated of their sympathetic or parasympathetic nerves to the submandibular gland. One week later, the expression of Na,K-ATPase, epithelial sodium channels (ENaC), and aquaporins (AQP) was examined in the denervated and contralateral glands. The sympathetic denervation slightly but significantly decreased the expression of ${\alpha}1$ subunit of Na,K-ATPase, whereas the parasympathetic denervation increased it. The expression of ${\alpha}$-subunit of ENaC was significantly increased in both the denervated and contralateral glands either by the sympathetic or parasympathetic denervation. The sympathetic denervation significantly increased the expression of AQP5 in both the denervated and contralateral glands, whereas the parasympathetic denervation decreased it. It is suggested that the autonomic nerves have a tonic effect on the regulation of sodium transporters and AQP water channels in the salivary gland.

• Journal of Chest Surgery
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• 제42권2호
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• pp.214-219
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• 2009

배경: 일차성 다한증은 교감신경의 과도한 활성을 특징으로 하고 흉강경을 통한 흉부 교감신경차단 술은 효과적, 안정적으로 시행되고 있다. 수술로 인한 교감신경과 부교감 신경의 불균형은 심장, 폐, 그 외 다른 여러 흉부장기에 영향을 준다. 저자들은 수술 후 나타나는 심박수와 심전도 변화를 분석하였다. 대상 및 방법: 우리는 1996년 10월부터 2006년 10월까지 수술 받은 263 명의 환자 중에 130명의 환자를 분석하였고 주된 수술부위에 따라 1, 2, 3군(1군: T2, 2군: T3, 3군: T4)으로 나누었고 수술 전과 후의 심전도를 비교하였다. 결과: 수술후 사망과 큰 합병증이 있는 환자는 없었고 심박수는 $71.6{\pm}10.6$회/분에서 $66.8{\pm}10.2$회/분으로 수술 후 감소하였고 PR 간격은 $148.6{\pm}21.2$ msec에서 $152.8{\pm}20.5$ msec으로 QTc 간격은 $399.2{\pm}15.4$ msec에서 $404.0{\pm}15.1$ msec으로 증가하였다. 흉부교감신경 차단부위에 따라 제1군에서는 심박동수와 QTc 간격이, 제2군에서는 심박동수와 PR간격이, 그리고 제 3군에서는 단지 QTc 간격이 수술전후에 의미 있게 변화하였다. 결론: 수술후 심박수와 심전도에 변화가 있었고 T2, T3, T4의 차단에 의하여 안정시 심장의 전기적 활성에 영향이 있었다.